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P38660

- PDIA6_MESAU

UniProt

P38660 - PDIA6_MESAU

Protein

Protein disulfide-isomerase A6

Gene

PDIA6

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin By similarity.By similarity

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei55 – 551NucleophileBy similarity
    Sitei56 – 561Contributes to redox potential valueBy similarity
    Sitei57 – 571Contributes to redox potential valueBy similarity
    Active sitei58 – 581NucleophileBy similarity
    Sitei118 – 1181Lowers pKa of C-terminal Cys of first active siteBy similarity
    Active sitei190 – 1901NucleophileBy similarity
    Sitei191 – 1911Contributes to redox potential valueBy similarity
    Sitei192 – 1921Contributes to redox potential valueBy similarity
    Active sitei193 – 1931NucleophileBy similarity
    Sitei256 – 2561Lowers pKa of C-terminal Cys of second active siteBy similarity

    GO - Molecular functioni

    1. protein disulfide isomerase activity Source: UniProtKB

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. platelet activation Source: UniProtKB
    3. platelet aggregation Source: UniProtKB
    4. protein folding Source: GOC

    Keywords - Molecular functioni

    Chaperone, Isomerase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide-isomerase A6 (EC:5.3.4.1)
    Alternative name(s):
    Protein disulfide isomerase P5
    Gene namesi
    Name:PDIA6
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation. Cell membrane By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. melanosome Source: UniProtKB
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Chaini20 – 439420Protein disulfide-isomerase A6PRO_0000034237Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi55 ↔ 58Redox-activePROSITE-ProRule annotation
    Disulfide bondi190 ↔ 193Redox-activePROSITE-ProRule annotation
    Modified residuei427 – 4271PhosphoserineBy similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    PRIDEiP38660.

    Expressioni

    Tissue specificityi

    Expressed most abundantly in lung and kidney, followed by heart, liver and brain.

    Interactioni

    Subunit structurei

    Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with ITGB3 following platelet stimulation By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP38660.
    SMRiP38660. Positions 20-132, 154-280.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 133114Thioredoxin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini151 – 287137Thioredoxin 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi436 – 4394Prevents secretion from ERPROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi421 – 43515Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Curated
    Contains 2 thioredoxin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Repeat, Signal

    Phylogenomic databases

    HOVERGENiHBG053548.

    Family and domain databases

    Gene3Di3.40.30.10. 2 hits.
    InterProiIPR005788. Disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 2 hits.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 3 hits.
    TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38660-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARLGFGLVS CTFFLAASGL YSSSDDVIEL TPSNFNREVI QSNSLWLVEF    50
    YAPWCGHCQR LTPEWKKAAT ALKDVVKVGA VDADKHQSLG GQYGVQGFPT 100
    IKIFGANKNK PEDYQGGRTG EAIVDAALSA LRQLVKDRLS GRSGGYSSGK 150
    QGRGDSSSKK DVIELTDDTF DKNVLDSDDV WMVEFYAPWC GHCKNLEPEW 200
    ATAATEVKEQ TKGKVKLAAV DATVNQVLAN RYGIRGFPTI KIFQKGEAPV 250
    DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDVAKKM CEEHQLCVVA 300
    VLPHILDTGA ARNSYLEILL KLADKYKKKM WGWLWTEAGA QSELENALGI 350
    GGFGYPAMAR INARKMKFAL LKGSFSEQGI NEFLRELSFG RASTAPVGGG 400
    SFPAITAREP WDGRDGELPV EDDIDLSDVE LDDLEKDEL 439
    Length:439
    Mass (Da):48,161
    Last modified:February 1, 1995 - v1
    Checksum:iE24CAECF5FFF5F8D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62678 mRNA. Translation: CAA44550.1.
    PIRiS19656.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62678 mRNA. Translation: CAA44550.1 .
    PIRi S19656.

    3D structure databases

    ProteinModelPortali P38660.
    SMRi P38660. Positions 20-132, 154-280.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P38660.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG053548.

    Family and domain databases

    Gene3Di 3.40.30.10. 2 hits.
    InterProi IPR005788. Disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 3 hits.
    TIGRFAMsi TIGR01126. pdi_dom. 2 hits.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The gene for a novel protein, a member of the protein disulphide isomerase/form I phosphoinositide-specific phospholipase C family, is amplified in hydroxyurea-resistant cells."
      Chaudhuri M.M., Tonin P.N., Lewis W.H., Srinivasan P.R.
      Biochem. J. 281:645-650(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-43.
    2. "Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa."
      Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S.
      Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPDIA6_MESAU
    AccessioniPrimary (citable) accession number: P38660
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3