Reviewed, UniProtKB/Swiss-Prot P38660 (PDIA6_MESAU)

Names and origin

Protein names

Recommended name:
    Protein disulfide-isomerase A6
    EC=5.3.4.1
Alternative name(s):
    Protein disulfide isomerase P5

Gene names

Name:

PDIA6

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity. Melanosome By similarity.
Tissue specificity	Expressed most abundantly in lung and kidney, followed by heart, liver and brain.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.1
	Chain	20 – 439	420	Protein disulfide-isomerase A6		PRO_0000034237
Regions
	Domain	20 – 133	114	Thioredoxin 1
	Domain	151 – 287	137	Thioredoxin 2
	Motif	436 – 439	4	Prevents secretion from ER By similarity
	Compositional bias	421 – 435	15	Asp/Glu-rich (acidic)
Sites
	Active site	55	1	Nucleophile By similarity
	Active site	58	1	Nucleophile By similarity
	Active site	190	1	Nucleophile By similarity
	Active site	193	1	Nucleophile By similarity
	Site	56	1	Contributes to redox potential value By similarity
	Site	57	1	Contributes to redox potential value By similarity
	Site	118	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	191	1	Contributes to redox potential value By similarity
	Site	192	1	Contributes to redox potential value By similarity
	Site	256	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Modified residue	427	1	Phosphoserine By similarity
	Disulfide bond	55 ↔ 58		Redox-active By similarity
	Disulfide bond	190 ↔ 193		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38660-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: E24CAECF5FFF5F8D	FASTA	439	48,161
	10 20 30 40 50 60 MARLGFGLVS CTFFLAASGL YSSSDDVIEL TPSNFNREVI QSNSLWLVEF YAPWCGHCQR 70 80 90 100 110 120 LTPEWKKAAT ALKDVVKVGA VDADKHQSLG GQYGVQGFPT IKIFGANKNK PEDYQGGRTG 130 140 150 160 170 180 EAIVDAALSA LRQLVKDRLS GRSGGYSSGK QGRGDSSSKK DVIELTDDTF DKNVLDSDDV 190 200 210 220 230 240 WMVEFYAPWC GHCKNLEPEW ATAATEVKEQ TKGKVKLAAV DATVNQVLAN RYGIRGFPTI 250 260 270 280 290 300 KIFQKGEAPV DYDGGRTRSD IVSRALDLFS DNAPPPELLE IINEDVAKKM CEEHQLCVVA 310 320 330 340 350 360 VLPHILDTGA ARNSYLEILL KLADKYKKKM WGWLWTEAGA QSELENALGI GGFGYPAMAR 370 380 390 400 410 420 INARKMKFAL LKGSFSEQGI NEFLRELSFG RASTAPVGGG SFPAITAREP WDGRDGELPV 430 EDDIDLSDVE LDDLEKDEL

References

[1]

"The gene for a novel protein, a member of the protein disulphide isomerase/form I phosphoinositide-specific phospholipase C family, is amplified in hydroxyurea-resistant cells." Chaudhuri M.M., Tonin P.N., Lewis W.H., Srinivasan P.R. Biochem. J. 281:645-650(1992) [PubMed: 1311171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-43.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62678 mRNA. Translation: CAA44550.1.
PIR	S19656.
3D structure databases
HSSP	HSSP built from PDB template 1MEK based on UniProtKB P07237.
SMR	P38660. Positions 20-132, 154-280.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P38660.
Enzyme and pathway databases
BRENDA	5.3.4.1. 824.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR000886. ER_target_seq_motif. IPR017936. Thioredoxin-like. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR01126. pdi_dom. 2 hits.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDIA6_MESAU

Accession

Primary (citable) accession number: P38660

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 22, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)