Reviewed, UniProtKB/Swiss-Prot P38658 (ERP60_SCHMA)

Names and origin

Protein names	Recommended name:     Probable protein disulfide-isomerase ER-60     EC=5.3.4.1 Alternative name(s):     ERP60
Organism	Schistosoma mansoni (Blood fluke)
Taxonomic identifier	6183 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 2 thioredoxin domains.
Caution	Was originally thought to be a phosphatidylinositol-4,5-bisphosphate phosphodiesterase type I (phospholipase C-alpha).

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Repeat Signal
Molecular function	Isomerase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein disulfide isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 14	14	Potential
	Chain	15 – 484	470	Probable protein disulfide-isomerase ER-60		PRO_0000034228
Regions
	Domain	15 – 125	111	Thioredoxin 1
	Domain	338 – 467	130	Thioredoxin 2
	Motif	481 – 484	4	Prevents secretion from ER By similarity
Sites
	Active site	46	1	Nucleophile By similarity
	Active site	49	1	Nucleophile By similarity
	Active site	388	1	Nucleophile By similarity
	Active site	391	1	Nucleophile By similarity
	Site	47	1	Contributes to redox potential value By similarity
	Site	48	1	Contributes to redox potential value By similarity
	Site	111	1	Lowers pKa of C-terminal Cys of first active site By similarity
	Site	389	1	Contributes to redox potential value By similarity
	Site	390	1	Contributes to redox potential value By similarity
	Site	453	1	Lowers pKa of C-terminal Cys of second active site By similarity
Amino acid modifications
	Disulfide bond	46 ↔ 49		Redox-active By similarity
	Disulfide bond	388 ↔ 391		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38658-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D85606CFC5C269B9	FASTA	484	54,306
	10 20 30 40 50 60 MRWLLSCLFL VAFASCSKVL ELTKDNFHSE LKSIPVALVK FYAPWCGHCK KLAPEFTSAA 70 80 90 100 110 120 QIISGKTNDV KLVKVDCTTQ ESICSEFGVS GYPTLKIFRN GDLDGEYNGP RNANGIANYM 130 140 150 160 170 180 ISRAGPVSKE VSTVSDVENV LSDDKPTVFA FVKSSSDPLI KTFMALAKSM VDDAVFCHSH 190 200 210 220 230 240 NNLFVTPSDN ELRVYLPKRL RTKFEDDFAV YKGELESNNI KDWIRKHGQG LVGYRSPSNT 250 260 270 280 290 300 FYFENSDLVV LYNNQSIDSY PSGVKYLRNR VLKTLKDNPQ KFKNLVFAYS FADDFSYEIS 310 320 330 340 350 360 DYGIEADKLP AVVIQSKDKK YKLEKFSLDA FSDFLNKFED GLLTPHVKSE PLPTDDSSAV 370 380 390 400 410 420 KKLVALNFDE IVNNEEKDVM VVFHAGWCGH CKNLMPKYEE AASKVKNEPN LVLAAMDATA 430 440 450 460 470 480 NDVPSPYQVR GFPTIYFVPK GKKSSPVSYE GGRDTNDIIK YLAREATEEL IGYDRSGNPK KSEL

References

[1]

"Characterization of the complete protein disulfide isomerase gene of Schistosoma mansoni and identification of the tissues of its expression." Finken M., Sobek A., Symmons P., Kunz W. Mol. Biochem. Parasitol. 64:135-144(1994) [PubMed: 8078516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Liberian.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z22934 Genomic DNA. Translation: CAA80521.1.
3D structure databases
HSSP	HSSP built from PDB template 1MEK based on UniProtKB P07237.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.3.4.1. 1460.
Family and domain databases
InterPro	IPR005788. Disulphide_isomerase. IPR005792. Prot_disulphide_isomerase. IPR017936. Thioredoxin-like. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00085. Thioredoxin. 2 hits. [Graphical view]
PRINTS	PR00421. THIOREDOXIN.
TIGRFAMs	TIGR01130. ER_PDI_fam. 1 hit. TIGR01126. pdi_dom. 2 hits.
PROSITE	PS00194. THIOREDOXIN_1. 2 hits. PS51352. THIOREDOXIN_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERP60_SCHMA

Accession

Primary (citable) accession number: P38658

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 22, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)