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P38649

- BGAT_MOUSE

UniProt

P38649 - BGAT_MOUSE

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Protein
Histo-blood group ABO system transferase
Gene
Abo
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.
UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

Cofactori

Binds 1 manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051UDP-N-acetyl-galactosamine By similarity
Metal bindingi190 – 1901Manganese By similarity
Metal bindingi192 – 1921Manganese By similarity
Binding sitei212 – 2121Glycoprotein fucosyl-galactosyl group By similarity
Binding sitei224 – 2241Glycoprotein fucosyl-galactosyl group By similarity
Binding sitei282 – 2821Glycoprotein fucosyl-galactosyl group By similarity
Binding sitei305 – 3051Glycoprotein fucosyl-galactosyl group By similarity

GO - Molecular functioni

  1. fucosylgalactoside 3-alpha-galactosyltransferase activity Source: MGI
  2. glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Source: MGI
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Histo-blood group ABO system transferase
Alternative name(s):
Cis-AB transferase
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40)
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37)
Histo-blood group A transferase
Short name:
A transferase
Histo-blood group B transferase
Short name:
B transferase
NAGAT
Gene namesi
Name:Abo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2135738. Abo.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted
Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei14 – 3421Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini35 – 332298Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Histo-blood group ABO system transferase
PRO_0000157293Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP38649.
PRIDEiP38649.

PTM databases

PhosphoSiteiP38649.

Expressioni

Tissue specificityi

Submaxillary glands (at protein level).1 Publication

Gene expression databases

BgeeiP38649.
GenevestigatoriP38649.

Structurei

3D structure databases

ProteinModelPortaliP38649.
SMRiP38649. Positions 52-324.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni100 – 1023UDP-N-acetyl-galactosamine binding By similarity
Regioni190 – 1923UDP-N-acetyl-galactosamine binding By similarity

Domaini

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG43612.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ9EQW3.
KOiK00709.
OrthoDBiEOG7BZVSQ.
PhylomeDBiP38649.
TreeFamiTF330991.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P38649-1 [UniParc]FASTAAdd to Basket

« Hide

MNLRGRPKCN FLHLGILPFA VFVLVFFGYL FLSFRSQNLG HPGAVTRNAY    50
LQPRVLKPTR KDVLVLTPWL APIIWEGTFN IDILNEQFRI RNTTIGLTVF 100
AIKKYVVFLK LFLETAEQHF MVGHKVIYYV FTDRPADVPQ VILGAGRQLV 150
VLTVRNYTRW QDVSMHRMEM ISHFSERRFL REVDYLVCAD ADMKFSDHVG 200
VEILSTFFGT LHPGFYSSSR EAFTYERRPQ SQAYIPWDRG DFYYGGAFFG 250
GSVLEVYHLT KACHEAMMED KANGIEPVWH DESYLNKYLL YHKPTKVLSP 300
EYLWDQQLLG WPSIMKKLRY VAVPKDHQAI RN 332
Length:332
Mass (Da):38,777
Last modified:December 16, 2008 - v2
Checksum:iC48DB1090EA0D3E4
GO

Sequence cautioni

The sequence CAM23695.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771G → R in BAC29005. 1 Publication
Sequence conflicti109 – 1091L → I in BAC29005. 1 Publication
Sequence conflicti160 – 1601W → C in BAC28473. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB041038 Genomic DNA. Translation: BAB20559.1.
AB041039 mRNA. Translation: BAB20560.1.
AK033786 mRNA. Translation: BAC28473.1.
AK035261 mRNA. Translation: BAC29005.1.
AL773563 Genomic DNA. Translation: CAM23695.1. Sequence problems.
AL773563 Genomic DNA. Translation: CAM23696.1.
CH466542 Genomic DNA. Translation: EDL08332.1.
BC116759 mRNA. Translation: AAI16760.1.
BC116761 mRNA. Translation: AAI16762.1.
CCDSiCCDS15811.1.
RefSeqiNP_001277373.1. NM_001290444.1.
NP_109643.3. NM_030718.5.
UniGeneiMm.160386.

Genome annotation databases

EnsembliENSMUST00000102900; ENSMUSP00000099964; ENSMUSG00000015787.
GeneIDi80908.
KEGGimmu:80908.
UCSCiuc008iwb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB041038 Genomic DNA. Translation: BAB20559.1 .
AB041039 mRNA. Translation: BAB20560.1 .
AK033786 mRNA. Translation: BAC28473.1 .
AK035261 mRNA. Translation: BAC29005.1 .
AL773563 Genomic DNA. Translation: CAM23695.1 . Sequence problems.
AL773563 Genomic DNA. Translation: CAM23696.1 .
CH466542 Genomic DNA. Translation: EDL08332.1 .
BC116759 mRNA. Translation: AAI16760.1 .
BC116761 mRNA. Translation: AAI16762.1 .
CCDSi CCDS15811.1.
RefSeqi NP_001277373.1. NM_001290444.1.
NP_109643.3. NM_030718.5.
UniGenei Mm.160386.

3D structure databases

ProteinModelPortali P38649.
SMRi P38649. Positions 52-324.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT6. Glycosyltransferase Family 6.

PTM databases

PhosphoSitei P38649.

Proteomic databases

PaxDbi P38649.
PRIDEi P38649.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102900 ; ENSMUSP00000099964 ; ENSMUSG00000015787 .
GeneIDi 80908.
KEGGi mmu:80908.
UCSCi uc008iwb.1. mouse.

Organism-specific databases

CTDi 28.
MGIi MGI:2135738. Abo.

Phylogenomic databases

eggNOGi NOG43612.
GeneTreei ENSGT00400000022032.
HOGENOMi HOG000234339.
HOVERGENi HBG003563.
InParanoidi Q9EQW3.
KOi K00709.
OrthoDBi EOG7BZVSQ.
PhylomeDBi P38649.
TreeFami TF330991.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 350270.
PROi P38649.
SOURCEi Search...

Gene expression databases

Bgeei P38649.
Genevestigatori P38649.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10462. PTHR10462. 1 hit.
Pfami PF03414. Glyco_transf_6. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Murine equivalent of the human histo-blood group ABO gene is a cis-AB gene and encodes a glycosyltransferase with both A and B transferase activity."
    Yamamoto M., Lin X.-H., Kominato Y., Hata Y., Noda R., Saitou N., Yamamoto F.
    J. Biol. Chem. 276:13701-13708(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Epididymis and Urinary bladder.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  6. "Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis."
    Merrick B.A., Patterson R.M., Wichter L.L., He C., Selkirk J.K.
    Electrophoresis 15:735-745(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY PARTIAL PROTEIN SEQUENCE.
    Tissue: Fibroblast.

Entry informationi

Entry nameiBGAT_MOUSE
AccessioniPrimary (citable) accession number: P38649
Secondary accession number(s): A2AL98
, Q8BZH3, Q8BZQ6, Q9EQW2, Q9EQW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 16, 2008
Last modified: July 9, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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