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P38649

- BGAT_MOUSE

UniProt

P38649 - BGAT_MOUSE

Protein

Histo-blood group ABO system transferase

Gene

Abo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (16 Dec 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.
    UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei105 – 1051UDP-N-acetyl-galactosamineBy similarity
    Metal bindingi190 – 1901ManganeseBy similarity
    Metal bindingi192 – 1921ManganeseBy similarity
    Binding sitei212 – 2121Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei224 – 2241Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei282 – 2821Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei305 – 3051Glycoprotein fucosyl-galactosyl groupBy similarity

    GO - Molecular functioni

    1. fucosylgalactoside 3-alpha-galactosyltransferase activity Source: MGI
    2. glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Source: MGI
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT6. Glycosyltransferase Family 6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histo-blood group ABO system transferase
    Alternative name(s):
    Cis-AB transferase
    Fucosylglycoprotein 3-alpha-galactosyltransferase
    Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
    Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40)
    Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37)
    Histo-blood group A transferase
    Short name:
    A transferase
    Histo-blood group B transferase
    Short name:
    B transferase
    NAGAT
    Gene namesi
    Name:Abo
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2135738. Abo.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted
    Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. Golgi cisterna membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332Histo-blood group ABO system transferasePRO_0000157293Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP38649.
    PRIDEiP38649.

    PTM databases

    PhosphoSiteiP38649.

    Expressioni

    Tissue specificityi

    Submaxillary glands (at protein level).1 Publication

    Gene expression databases

    BgeeiP38649.
    GenevestigatoriP38649.

    Structurei

    3D structure databases

    ProteinModelPortaliP38649.
    SMRiP38649. Positions 52-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1313CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini35 – 332298LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei14 – 3421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni100 – 1023UDP-N-acetyl-galactosamine bindingBy similarity
    Regioni190 – 1923UDP-N-acetyl-galactosamine bindingBy similarity

    Domaini

    The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

    Sequence similaritiesi

    Belongs to the glycosyltransferase 6 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43612.
    GeneTreeiENSGT00400000022032.
    HOGENOMiHOG000234339.
    HOVERGENiHBG003563.
    InParanoidiQ9EQW3.
    KOiK00709.
    OrthoDBiEOG7BZVSQ.
    PhylomeDBiP38649.
    TreeFamiTF330991.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005076. Glyco_trans_6.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10462. PTHR10462. 1 hit.
    PfamiPF03414. Glyco_transf_6. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38649-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLRGRPKCN FLHLGILPFA VFVLVFFGYL FLSFRSQNLG HPGAVTRNAY    50
    LQPRVLKPTR KDVLVLTPWL APIIWEGTFN IDILNEQFRI RNTTIGLTVF 100
    AIKKYVVFLK LFLETAEQHF MVGHKVIYYV FTDRPADVPQ VILGAGRQLV 150
    VLTVRNYTRW QDVSMHRMEM ISHFSERRFL REVDYLVCAD ADMKFSDHVG 200
    VEILSTFFGT LHPGFYSSSR EAFTYERRPQ SQAYIPWDRG DFYYGGAFFG 250
    GSVLEVYHLT KACHEAMMED KANGIEPVWH DESYLNKYLL YHKPTKVLSP 300
    EYLWDQQLLG WPSIMKKLRY VAVPKDHQAI RN 332
    Length:332
    Mass (Da):38,777
    Last modified:December 16, 2008 - v2
    Checksum:iC48DB1090EA0D3E4
    GO

    Sequence cautioni

    The sequence CAM23695.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti77 – 771G → R in BAC29005. (PubMed:16141072)Curated
    Sequence conflicti109 – 1091L → I in BAC29005. (PubMed:16141072)Curated
    Sequence conflicti160 – 1601W → C in BAC28473. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB041038 Genomic DNA. Translation: BAB20559.1.
    AB041039 mRNA. Translation: BAB20560.1.
    AK033786 mRNA. Translation: BAC28473.1.
    AK035261 mRNA. Translation: BAC29005.1.
    AL773563 Genomic DNA. Translation: CAM23695.1. Sequence problems.
    AL773563 Genomic DNA. Translation: CAM23696.1.
    CH466542 Genomic DNA. Translation: EDL08332.1.
    BC116759 mRNA. Translation: AAI16760.1.
    BC116761 mRNA. Translation: AAI16762.1.
    CCDSiCCDS15811.1.
    RefSeqiNP_001277373.1. NM_001290444.1.
    NP_109643.3. NM_030718.5.
    UniGeneiMm.160386.

    Genome annotation databases

    EnsembliENSMUST00000102900; ENSMUSP00000099964; ENSMUSG00000015787.
    GeneIDi80908.
    KEGGimmu:80908.
    UCSCiuc008iwb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB041038 Genomic DNA. Translation: BAB20559.1 .
    AB041039 mRNA. Translation: BAB20560.1 .
    AK033786 mRNA. Translation: BAC28473.1 .
    AK035261 mRNA. Translation: BAC29005.1 .
    AL773563 Genomic DNA. Translation: CAM23695.1 . Sequence problems.
    AL773563 Genomic DNA. Translation: CAM23696.1 .
    CH466542 Genomic DNA. Translation: EDL08332.1 .
    BC116759 mRNA. Translation: AAI16760.1 .
    BC116761 mRNA. Translation: AAI16762.1 .
    CCDSi CCDS15811.1.
    RefSeqi NP_001277373.1. NM_001290444.1.
    NP_109643.3. NM_030718.5.
    UniGenei Mm.160386.

    3D structure databases

    ProteinModelPortali P38649.
    SMRi P38649. Positions 52-324.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT6. Glycosyltransferase Family 6.

    PTM databases

    PhosphoSitei P38649.

    Proteomic databases

    PaxDbi P38649.
    PRIDEi P38649.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102900 ; ENSMUSP00000099964 ; ENSMUSG00000015787 .
    GeneIDi 80908.
    KEGGi mmu:80908.
    UCSCi uc008iwb.1. mouse.

    Organism-specific databases

    CTDi 28.
    MGIi MGI:2135738. Abo.

    Phylogenomic databases

    eggNOGi NOG43612.
    GeneTreei ENSGT00400000022032.
    HOGENOMi HOG000234339.
    HOVERGENi HBG003563.
    InParanoidi Q9EQW3.
    KOi K00709.
    OrthoDBi EOG7BZVSQ.
    PhylomeDBi P38649.
    TreeFami TF330991.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 350270.
    PROi P38649.
    SOURCEi Search...

    Gene expression databases

    Bgeei P38649.
    Genevestigatori P38649.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005076. Glyco_trans_6.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10462. PTHR10462. 1 hit.
    Pfami PF03414. Glyco_transf_6. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Murine equivalent of the human histo-blood group ABO gene is a cis-AB gene and encodes a glycosyltransferase with both A and B transferase activity."
      Yamamoto M., Lin X.-H., Kominato Y., Hata Y., Noda R., Saitou N., Yamamoto F.
      J. Biol. Chem. 276:13701-13708(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Epididymis and Urinary bladder.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    6. "Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis."
      Merrick B.A., Patterson R.M., Wichter L.L., He C., Selkirk J.K.
      Electrophoresis 15:735-745(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY PARTIAL PROTEIN SEQUENCE.
      Tissue: Fibroblast.

    Entry informationi

    Entry nameiBGAT_MOUSE
    AccessioniPrimary (citable) accession number: P38649
    Secondary accession number(s): A2AL98
    , Q8BZH3, Q8BZQ6, Q9EQW2, Q9EQW3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: December 16, 2008
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3