Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38649 (BGAT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histo-blood group ABO system transferase
Alternative name(s):
Cis-AB transferase
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
EC=2.4.1.40
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
EC=2.4.1.37
Histo-blood group A transferase
Short name=A transferase
Histo-blood group B transferase
Short name=B transferase
NAGAT
Gene names
Name:Abo
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.

UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

Cofactor

Binds 1 manganese ion per subunit By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.

Tissue specificity

Submaxillary glands (at protein level). Ref.1

Domain

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similarities

Belongs to the glycosyltransferase 6 family.

Sequence caution

The sequence CAM23695.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Histo-blood group ABO system transferase
PRO_0000157293

Regions

Topological domain1 – 1313Cytoplasmic Potential
Transmembrane14 – 3421Helical; Signal-anchor for type II membrane protein; Potential
Topological domain35 – 332298Lumenal Potential
Region100 – 1023UDP-N-acetyl-galactosamine binding By similarity
Region190 – 1923UDP-N-acetyl-galactosamine binding By similarity

Sites

Metal binding1901Manganese By similarity
Metal binding1921Manganese By similarity
Binding site1051UDP-N-acetyl-galactosamine By similarity
Binding site2121Glycoprotein fucosyl-galactosyl group By similarity
Binding site2241Glycoprotein fucosyl-galactosyl group By similarity
Binding site2821Glycoprotein fucosyl-galactosyl group By similarity
Binding site3051Glycoprotein fucosyl-galactosyl group By similarity

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict771G → R in BAC29005. Ref.2
Sequence conflict1091L → I in BAC29005. Ref.2
Sequence conflict1601W → C in BAC28473. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P38649 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: C48DB1090EA0D3E4

FASTA33238,777
        10         20         30         40         50         60 
MNLRGRPKCN FLHLGILPFA VFVLVFFGYL FLSFRSQNLG HPGAVTRNAY LQPRVLKPTR 

        70         80         90        100        110        120 
KDVLVLTPWL APIIWEGTFN IDILNEQFRI RNTTIGLTVF AIKKYVVFLK LFLETAEQHF 

       130        140        150        160        170        180 
MVGHKVIYYV FTDRPADVPQ VILGAGRQLV VLTVRNYTRW QDVSMHRMEM ISHFSERRFL 

       190        200        210        220        230        240 
REVDYLVCAD ADMKFSDHVG VEILSTFFGT LHPGFYSSSR EAFTYERRPQ SQAYIPWDRG 

       250        260        270        280        290        300 
DFYYGGAFFG GSVLEVYHLT KACHEAMMED KANGIEPVWH DESYLNKYLL YHKPTKVLSP 

       310        320        330 
EYLWDQQLLG WPSIMKKLRY VAVPKDHQAI RN 

« Hide

References

« Hide 'large scale' references
[1]"Murine equivalent of the human histo-blood group ABO gene is a cis-AB gene and encodes a glycosyltransferase with both A and B transferase activity."
Yamamoto M., Lin X.-H., Kominato Y., Hata Y., Noda R., Saitou N., Yamamoto F.
J. Biol. Chem. 276:13701-13708(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Epididymis and Urinary bladder.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[6]"Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis."
Merrick B.A., Patterson R.M., Wichter L.L., He C., Selkirk J.K.
Electrophoresis 15:735-745(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY PARTIAL PROTEIN SEQUENCE.
Tissue: Fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB041038 Genomic DNA. Translation: BAB20559.1.
AB041039 mRNA. Translation: BAB20560.1.
AK033786 mRNA. Translation: BAC28473.1.
AK035261 mRNA. Translation: BAC29005.1.
AL773563 Genomic DNA. Translation: CAM23695.1. Sequence problems.
AL773563 Genomic DNA. Translation: CAM23696.1.
CH466542 Genomic DNA. Translation: EDL08332.1.
BC116759 mRNA. Translation: AAI16760.1.
BC116761 mRNA. Translation: AAI16762.1.
RefSeqNP_109643.3. NM_030718.4.
UniGeneMm.160386.

3D structure databases

ProteinModelPortalP38649.
SMRP38649. Positions 44-325.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT6. Glycosyltransferase Family 6.

PTM databases

PhosphoSiteP38649.

Proteomic databases

PaxDbP38649.
PRIDEP38649.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102900; ENSMUSP00000099964; ENSMUSG00000015787.
GeneID80908.
KEGGmmu:80908.
UCSCuc008iwb.1. mouse.

Organism-specific databases

CTD28.
MGIMGI:2135738. Abo.

Phylogenomic databases

eggNOGNOG43612.
GeneTreeENSGT00400000022032.
HOGENOMHOG000234339.
HOVERGENHBG003563.
InParanoidQ9EQW3.
KOK00709.
OrthoDBEOG7BZVSQ.
PhylomeDBP38649.
TreeFamTF330991.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeP38649.
GenevestigatorP38649.

Family and domain databases

InterProIPR005076. Glyco_trans_6.
[Graphical view]
PANTHERPTHR10462. PTHR10462. 1 hit.
PfamPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio350270.
PROP38649.
SOURCESearch...

Entry information

Entry nameBGAT_MOUSE
AccessionPrimary (citable) accession number: P38649
Secondary accession number(s): A2AL98 expand/collapse secondary AC list , Q8BZH3, Q8BZQ6, Q9EQW2, Q9EQW3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 16, 2008
Last modified: April 16, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot