P38647 (GRP75_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Stress-70 protein, mitochondrial

Alternative name(s):
75 kDa glucose-regulated protein
Short name=GRP-75
Heat shock 70 kDa protein 9
Mortalin
Peptide-binding protein 74
Short name=PBP74
p66 MOT

Gene names

Name:	Hspa9
Synonyms:	Grp75, Hsp74, Hspa9a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	679 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone.
Subunit structure	Interacts with FXN. Interacts with HSCB By similarity. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 By similarity.
Subcellular location	Mitochondrion. Nucleus › nucleolus By similarity Ref.13.
Tissue specificity	Found in all the cell types examined.
Induction	Not induced by heat shock, instead protein level is decreased.
Polymorphism	Two forms of the protein have been found, MOT-1, found in mortal cells and MOT-2, found in immortal cells. The sequence of MOT-2 is shown here.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Cellular component	Mitochondrion Nucleus
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein export from nucleus Inferred from direct assay PubMed 11900485. Source: MGI protein folding Inferred from electronic annotation. Source: InterPro
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara mitochondrial nucleoid Inferred from electronic annotation. Source: Compara mitochondrion Inferred from direct assay PubMed 12931191PubMed 14651853PubMed 14993262PubMed 18614015. Source: MGI nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 46	46	Mitochondrion Ref.11
	Chain	47 – 679	633	Stress-70 protein, mitochondrial		PRO_0000013564
Amino acid modifications
	Modified residue	135	1	N6-acetyllysine By similarity
	Modified residue	138	1	N6-acetyllysine By similarity
	Modified residue	143	1	N6-acetyllysine By similarity
	Modified residue	206	1	N6-malonyllysine By similarity
	Modified residue	234	1	N6-acetyllysine By similarity
	Modified residue	288	1	N6-acetyllysine Ref.14
	Modified residue	300	1	N6-acetyllysine Ref.14
	Modified residue	360	1	N6-acetyllysine Ref.14
	Modified residue	567	1	N6-acetyllysine By similarity
	Modified residue	646	1	N6-acetyllysine By similarity
Natural variations
	Natural variant	618	1	M → V in MOT-1.
	Natural variant	624	1	G → R in MOT-1.
Experimental info
	Sequence conflict	5	1	S → T AA sequence Ref.4
	Sequence conflict	78	1	L → Q in BAE21690. Ref.7
	Sequence conflict	106	1	K → R AA sequence Ref.4
	Sequence conflict	150	1	G → S in BAE35373. Ref.7
	Sequence conflict	522	1	F → S AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P38647 [UniParc]. Last modified October 3, 2012. Version 3. Checksum: DF1C997775627928	FASTA	679	73,461
	10 20 30 40 50 60 MISASRAAAA RLVGTAASRS PAAARPQDGW NGLSHEAFRF VSRRDYASEA IKGAVVGIDL 70 80 90 100 110 120 GTTNSCVAVM EGKQAKVLEN AEGARTTPSV VAFTADGERL VGMPAKRQAV TNPNNTFYAT 130 140 150 160 170 180 KRLIGRRYDD PEVQKDTKNV PFKIVRASNG DAWVEAHGKL YSPSQIGAFV LMKMKETAEN 190 200 210 220 230 240 YLGHTAKNAV ITVPAYFNDS QRQATKDAGQ ISGLNVLRVI NEPTAAALAY GLDKSEDKVI 250 260 270 280 290 300 AVYDLGGGTF DISILEIQKG VFEVKSTNGD TFLGGEDFDQ ALLRHIVKEF KRETGVDLTK 310 320 330 340 350 360 DNMALQRVRE AAEKAKCELS SSVQTDINLP YLTMDASGPK HLNMKLTRAQ FEGIVTDLIK 370 380 390 400 410 420 RTIAPCQKAM QDAEVSKSDI GEVILVGGMT RMPKVQQTVQ DLFGRAPSKA VNPDEAVAIG 430 440 450 460 470 480 AAIQGGVLAG DVTDVLLLDV TPLSLGIETL GGVFTKLINR NTTIPTKKSQ VFSTAADGQT 490 500 510 520 530 540 QVEIKVCQGE REMAGDNKLL GQFTLIGIPP APRGVPQIEV TFDIDANGIV HVSAKDKGTG 550 560 570 580 590 600 REQQIVIQSS GGLSKDDIEN MVKNAEKYAE EDRRKKERVE AVNMAEGIIH DTETKMEEFK 610 620 630 640 650 660 DQLPADECNK LKEEISKMRA LLAGKDSETG ENIRQAASSL QQASLKLFEM AYKKMASERE 670 GSGSSGTGEQ KEDQKEEKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel member of mouse hsp70 family. Its association with cellular mortal phenotype." Wadhwa R., Kaul S.C., Ikawa Y., Sugimoto Y. J. Biol. Chem. 268:6615-6621(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1-ICR. Tissue: Embryonic fibroblast.
[2]	Wadhwa R. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 123.
[3]	"Induction of cellular senescence by transfection of cytosolic mortalin cDNA in NIH 3T3 cells." Wadhwa R., Kaul S.C., Sugimoto Y., Mitsui Y. J. Biol. Chem. 268:22239-22242(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: CD-1-ICR. Tissue: Embryonic fibroblast.
[4]	"Cloning of the gene encoding peptide-binding protein 74 shows that it is a new member of the heat shock protein 70 family." Domanico S.Z., Denagel D.C., Dahlseid J.N., Green J.M., Pierce S.K. Mol. Cell. Biol. 13:3598-3610(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: B-cell.
[5]	"Structure and organization of the gene encoding a mouse mitochondrial stress-70 protein." Michikawa Y., Baba T., Arai Y., Sakakura T., Kusakabe M. FEBS Lett. 336:27-33(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Liver.
[6]	"Antigenic protein specific for C3H strain mouse is a mitochondrial stress-70 protein." Michikawa Y., Baba T., Arai Y., Sakakura T., Tanaka M., Kusakabe M. Biochem. Biophys. Res. Commun. 196:223-232(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H/HeN. Tissue: Kidney.
[7]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and DBA/2. Tissue: Embryo, Kidney and Liver.
[8]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[9]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[11]	"Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis." Merrick B.A., Patterson R.M., Wichter L.L., He C., Selkirk J.K. Electrophoresis 15:735-745(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 47-70. Tissue: Fibroblast.
[12]	Lubec G., Klug S., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 188-202; 266-284; 349-360; 395-405 AND 499-513, MASS SPECTROMETRY. Tissue: Brain and Hippocampus.
[13]	"PBP74, a new member of the mammalian 70-kDa heat shock protein family, is a mitochondrial protein." Dahlseid J.N., Lill R., Green J.M., Xu X., Qiu Y., Pierce S.K. Mol. Biol. Cell 5:1265-1275(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[14]	"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-288; LYS-300 AND LYS-360, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11089 mRNA. Translation: BAA01862.2. L06896 mRNA. No translation available. D17666 Genomic DNA. Translation: BAA04548.1. D17556 mRNA. Translation: BAA04493.1. AK004946 mRNA. Translation: BAB23690.1. AK002634 mRNA. Translation: BAB22248.1. AK133501 mRNA. Translation: BAE21690.1. AK137109 mRNA. Translation: BAE23238.1. AK145965 mRNA. Translation: BAE26790.1. AK159791 mRNA. Translation: BAE35373.1. AK165958 mRNA. Translation: BAE38486.1. AK167856 mRNA. Translation: BAE39874.1. AC114820 Genomic DNA. No translation available. AC131675 Genomic DNA. No translation available. CH466557 Genomic DNA. Translation: EDK97122.1. BC052727 mRNA. Translation: AAH52727.1. BC057343 mRNA. Translation: AAH57343.1.
IPI	IPI00133903. IPI00880839.
PIR	A48127. S39839.
RefSeq	NP_034611.2. NM_010481.2.
UniGene	Mm.209419.
3D structure databases
ProteinModelPortal	P38647.
SMR	P38647. Positions 55-651.
ModBase	Search...
Protein-protein interaction databases
IntAct	P38647. 13 interactions.
STRING	10090.ENSMUSP00000025217.
PTM databases
PhosphoSite	P38647.
2D gel databases
COMPLUYEAST-2DPAGE	P38647.
REPRODUCTION-2DPAGE	IPI00133903. P38647.
SWISS-2DPAGE	P38647.
Proteomic databases
PaxDb	P38647.
PRIDE	P38647.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025217; ENSMUSP00000025217; ENSMUSG00000024359.
GeneID	15526.
KEGG	mmu:15526.
Organism-specific databases
CTD	3313.
MGI	MGI:96245. Hspa9.
Phylogenomic databases
eggNOG	COG0443.
GeneTree	ENSGT00550000074813.
HOGENOM	HOG000228136.
HOVERGEN	HBG051845.
InParanoid	P38647.
KO	K04043.
OMA	PYKNINP.
OrthoDB	EOG49KFQ6.
Gene expression databases
CleanEx	MM_HSPA9.
Genevestigator	P38647.
GermOnline	ENSMUSG00000024359. Mus musculus.
Family and domain databases
InterPro	IPR012725. Chaperone_DnaK. IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
TIGRFAMs	TIGR02350. prok_dnaK. 1 hit.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HSPA9. mouse.
NextBio	288444.
SOURCE	Search...

Entry information

Entry name

GRP75_MOUSE

Accession

Primary (citable) accession number: P38647
Secondary accession number(s): Q3TW93 , Q3UVN1, Q3V015, Q7TSZ0, Q9CQ05

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 127 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program