Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P38645

- BGLB_THEBI

UniProt

P38645 - BGLB_THEBI

Protein

Thermostable beta-glucosidase B

Gene

bglB

Organism
Thermobispora bispora (Microbispora bispora)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Temperature dependencei

    Thermostable, retains about 70% of its activity at 60 degrees Celsius.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei196 – 1961Proton donorSequence Analysis
    Active sitei378 – 3781NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thermostable beta-glucosidase B (EC:3.2.1.21)
    Alternative name(s):
    Amygdalase
    Beta-D-glucoside glucohydrolase
    Cellobiase
    Gentiobiase
    Gene namesi
    Name:bglB
    OrganismiThermobispora bispora (Microbispora bispora)
    Taxonomic identifieri2006 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeThermobispora

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Thermostable beta-glucosidase BPRO_0000063878Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP38645.
    SMRiP38645. Positions 40-467.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR017736. Glyco_hydro_1_beta-glucosidase.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR03356. BGL. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38645-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTESAMTSRA GRGRGADLVA AVVQGHAAAS DAAGDLSFPD GFIWGAATAA    50
    YQIEGAWRED GRGLWDVFSH TPGKVASGHT GDIACDHYHR YADDVRLMAG 100
    LGDRVYRFSV AWPRIVPDGS GPVNPAGLDF YDRLVDELLG HGITPYPTLY 150
    HWDLPQTLED RGGWAARDTA YRFAEYALAV HRRLGDRVRC WITLNEPWVA 200
    AFLATHRGAP GAADVPRFRA VHHLLLGHGL GLRLRSAGAG QLGLTLSLSP 250
    VIEARPGVRG GGRRVDALAN RQFLDPALRG RYPEEVLKIM AGHARLGHPG 300
    RDLETIHQPV DLLGVNYYSH VRLAAEGEPA NRLPGSEGIR FERPTAVTAW 350
    PGDRPDGLRT LLLRLSRDYP GVGLIITENG AAFDDRADGD RVHDPERIRY 400
    LTATLRAVHD AIMAGADLRG YFVWSVLDNF EWAYGYHKRG IVYVDYTTMR 450
    RIPRESALWY RDVVRRNGLR NGE 473
    Length:473
    Mass (Da):52,228
    Last modified:October 1, 1994 - v1
    Checksum:i8F2188F1C93007CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97265 Genomic DNA. Translation: AAA25311.1.
    PIRiA48949.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97265 Genomic DNA. Translation: AAA25311.1 .
    PIRi A48949.

    3D structure databases

    ProteinModelPortali P38645.
    SMRi P38645. Positions 40-467.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR017736. Glyco_hydro_1_beta-glucosidase.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    TIGRFAMsi TIGR03356. BGL. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization, and nucleotide sequence of a gene encoding Microbispora bispora BglB, a thermostable beta-glucosidase expressed in Escherichia coli."
      Wright R.M., Yablonsky M.D., Shalita Z.P., Goyal A.K., Eveleigh D.E.
      Appl. Environ. Microbiol. 58:3455-3465(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NRRL 15568.

    Entry informationi

    Entry nameiBGLB_THEBI
    AccessioniPrimary (citable) accession number: P38645
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Shows greater activity against cellobiose than against aryl-beta-D-glucosides.

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3