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Protein

Thermostable beta-glucosidase B

Gene

bglB

Organism
Thermobispora bispora (Microbispora bispora)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Temperature dependencei

Thermostable, retains about 70% of its activity at 60 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei196 – 1961Proton donorSequence Analysis
Active sitei378 – 3781NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermostable beta-glucosidase B (EC:3.2.1.21)
Alternative name(s):
Amygdalase
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase
Gene namesi
Name:bglB
OrganismiThermobispora bispora (Microbispora bispora)
Taxonomic identifieri2006 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeThermobispora

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Thermostable beta-glucosidase BPRO_0000063878Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP38645.
SMRiP38645. Positions 40-467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR03356. BGL. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESAMTSRA GRGRGADLVA AVVQGHAAAS DAAGDLSFPD GFIWGAATAA
60 70 80 90 100
YQIEGAWRED GRGLWDVFSH TPGKVASGHT GDIACDHYHR YADDVRLMAG
110 120 130 140 150
LGDRVYRFSV AWPRIVPDGS GPVNPAGLDF YDRLVDELLG HGITPYPTLY
160 170 180 190 200
HWDLPQTLED RGGWAARDTA YRFAEYALAV HRRLGDRVRC WITLNEPWVA
210 220 230 240 250
AFLATHRGAP GAADVPRFRA VHHLLLGHGL GLRLRSAGAG QLGLTLSLSP
260 270 280 290 300
VIEARPGVRG GGRRVDALAN RQFLDPALRG RYPEEVLKIM AGHARLGHPG
310 320 330 340 350
RDLETIHQPV DLLGVNYYSH VRLAAEGEPA NRLPGSEGIR FERPTAVTAW
360 370 380 390 400
PGDRPDGLRT LLLRLSRDYP GVGLIITENG AAFDDRADGD RVHDPERIRY
410 420 430 440 450
LTATLRAVHD AIMAGADLRG YFVWSVLDNF EWAYGYHKRG IVYVDYTTMR
460 470
RIPRESALWY RDVVRRNGLR NGE
Length:473
Mass (Da):52,228
Last modified:October 1, 1994 - v1
Checksum:i8F2188F1C93007CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97265 Genomic DNA. Translation: AAA25311.1.
PIRiA48949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97265 Genomic DNA. Translation: AAA25311.1.
PIRiA48949.

3D structure databases

ProteinModelPortaliP38645.
SMRiP38645. Positions 40-467.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR03356. BGL. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, characterization, and nucleotide sequence of a gene encoding Microbispora bispora BglB, a thermostable beta-glucosidase expressed in Escherichia coli."
    Wright R.M., Yablonsky M.D., Shalita Z.P., Goyal A.K., Eveleigh D.E.
    Appl. Environ. Microbiol. 58:3455-3465(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NRRL 15568.

Entry informationi

Entry nameiBGLB_THEBI
AccessioniPrimary (citable) accession number: P38645
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Shows greater activity against cellobiose than against aryl-beta-D-glucosides.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.