Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P38645

- BGLB_THEBI

UniProt

P38645 - BGLB_THEBI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Thermostable beta-glucosidase B

Gene

bglB

Organism
Thermobispora bispora (Microbispora bispora)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Temperature dependencei

Thermostable, retains about 70% of its activity at 60 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei196 – 1961Proton donorSequence Analysis
Active sitei378 – 3781NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermostable beta-glucosidase B (EC:3.2.1.21)
Alternative name(s):
Amygdalase
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase
Gene namesi
Name:bglB
OrganismiThermobispora bispora (Microbispora bispora)
Taxonomic identifieri2006 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeThermobispora

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Thermostable beta-glucosidase BPRO_0000063878Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP38645.
SMRiP38645. Positions 40-467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR03356. BGL. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38645-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTESAMTSRA GRGRGADLVA AVVQGHAAAS DAAGDLSFPD GFIWGAATAA
60 70 80 90 100
YQIEGAWRED GRGLWDVFSH TPGKVASGHT GDIACDHYHR YADDVRLMAG
110 120 130 140 150
LGDRVYRFSV AWPRIVPDGS GPVNPAGLDF YDRLVDELLG HGITPYPTLY
160 170 180 190 200
HWDLPQTLED RGGWAARDTA YRFAEYALAV HRRLGDRVRC WITLNEPWVA
210 220 230 240 250
AFLATHRGAP GAADVPRFRA VHHLLLGHGL GLRLRSAGAG QLGLTLSLSP
260 270 280 290 300
VIEARPGVRG GGRRVDALAN RQFLDPALRG RYPEEVLKIM AGHARLGHPG
310 320 330 340 350
RDLETIHQPV DLLGVNYYSH VRLAAEGEPA NRLPGSEGIR FERPTAVTAW
360 370 380 390 400
PGDRPDGLRT LLLRLSRDYP GVGLIITENG AAFDDRADGD RVHDPERIRY
410 420 430 440 450
LTATLRAVHD AIMAGADLRG YFVWSVLDNF EWAYGYHKRG IVYVDYTTMR
460 470
RIPRESALWY RDVVRRNGLR NGE
Length:473
Mass (Da):52,228
Last modified:October 1, 1994 - v1
Checksum:i8F2188F1C93007CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97265 Genomic DNA. Translation: AAA25311.1.
PIRiA48949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97265 Genomic DNA. Translation: AAA25311.1 .
PIRi A48949.

3D structure databases

ProteinModelPortali P38645.
SMRi P38645. Positions 40-467.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
TIGRFAMsi TIGR03356. BGL. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, characterization, and nucleotide sequence of a gene encoding Microbispora bispora BglB, a thermostable beta-glucosidase expressed in Escherichia coli."
    Wright R.M., Yablonsky M.D., Shalita Z.P., Goyal A.K., Eveleigh D.E.
    Appl. Environ. Microbiol. 58:3455-3465(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NRRL 15568.

Entry informationi

Entry nameiBGLB_THEBI
AccessioniPrimary (citable) accession number: P38645
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Shows greater activity against cellobiose than against aryl-beta-D-glucosides.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3