P38636 (ATX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metal homeostasis factor ATX1

Gene names

Name:	ATX1
Ordered Locus Names:	YNL259C
ORF Names:	N0840

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	73 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Shuttles copper to the transport ATPase CCC2. Protects against oxygen toxicity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the ATX1 family. Contains 1 HMA domain.

Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Cellular component	Cytoplasm
Ligand	Copper Metal-binding
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular iron ion homeostasis Inferred from mutant phenotype PubMed 9083054. Source: SGD cellular response to oxidative stress Inferred from mutant phenotype Ref.1. Source: SGD copper ion transport Inferred from mutant phenotype PubMed 9083054. Source: SGD
Cellular_component	cytosol Inferred from direct assay PubMed 9083054. Source: SGD
Molecular_function	copper chaperone activity Inferred from direct assay PubMed 17961510PubMed 9346482. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PRO3	P32263	1	EBI-2046026,EBI-13885
PSA1	P41940	1	EBI-2046026,EBI-11191
RNR2	P09938	1	EBI-2046026,EBI-15240

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 73

73

Metal homeostasis factor ATX1

PRO_0000212535

Regions

Domain

5 – 69

65

HMA

Sites

Metal binding

15

1

Copper

Metal binding

18

1

Copper

Experimental info

Sequence conflict

8

1

Q → H in AAS56693. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

73

Helix Strand Turn

Details...

Beta strand

5 – 11

7

Helix

16 – 27

12

Turn

28 – 31

4

Beta strand

32 – 39

8

Turn

40 – 43

4

Beta strand

44 – 51

8

Helix

53 – 61

9

Turn

62 – 64

3

Beta strand

67 – 73

7

Sequences

	Sequence		Length	Mass (Da)
	P38636 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 9BDF0B542F80EA83	FASTA	73	8,221
	10 20 30 40 50 60 MAEIKHYQFN VVMTCSGCSG AVNKVLTKLE PDVSKIDISL EKQLVDVYTT LPYDFILEKI 70 KKTGKEVRSG KQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ATX1 gene of Saccharomyces cerevisiae encodes a small metal homeostasis factor that protects cells against reactive oxygen toxicity." Lin S.-J., Culotta V.L. Proc. Natl. Acad. Sci. U.S.A. 92:3784-3788(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae." Sen-Gupta M., Gueldener U., Beinhauer J.D., Fiedler T.A., Hegemann J.H. Yeast 13:849-860(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Crystal structure of the Atx1 metallochaperone protein at 1.02 A resolution." Rosenzweig A.C., Huffman D.L., Hou M.Y., Wernimont A.K., Pufahl R.A., O'Halloran T.V. Structure 7:605-617(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.02 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L35270 Genomic DNA. Translation: AAC37428.1.
X96722 Genomic DNA. Translation: CAA65485.1.
Z71535 Genomic DNA. Translation: CAA96166.1.
AY558367 Genomic DNA. Translation: AAS56693.1.
BK006947 Genomic DNA. Translation: DAA10300.1.

PIR

S47930.

RefSeq

NP_014140.1. NM_001183097.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CC7	X-ray	1.20	A	1-73	[»]
1CC8	X-ray	1.02	A	1-73	[»]
1FD8	NMR	-	A	1-73	[»]
1FES	NMR	-	A	1-73	[»]
1UV1	model	-	A	1-73	[»]
1UV2	model	-	A	1-73	[»]
2GGP	NMR	-	A	1-73	[»]
3K7R	X-ray	2.28	A/B/C/D/E/F/G/H/I/J/K/L	1-73	[»]

ProteinModelPortal

P38636.

SMR

P38636. Positions 2-73.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-813N.

IntAct

P38636. 2 interactions.

MINT

MINT-367601.

STRING

4932.YNL259C.

Proteomic databases

PaxDb

P38636.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YNL259C; YNL259C; YNL259C.

GeneID

855462.

KEGG

sce:YNL259C.

Organism-specific databases

CYGD

YNL259c.

SGD

S000005203. ATX1.

Phylogenomic databases

eggNOG

COG2608.

GeneTree

ENSGT00600000085316.

HOGENOM

HOG000038877.

KO

K07213.

OMA

QTVDVYT.

OrthoDB

EOG418FZ8.

Gene expression databases

Genevestigator

P38636.

GermOnline

YNL259C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe-assoc_HMA.
[Graphical view]

Pfam

PF00403. HMA. 1 hit.
[Graphical view]

SUPFAM

SSF55008. HeavyMe_transpt. 1 hit.

PROSITE

PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P38636.

NextBio

979391.

Entry information

Entry name

ATX1_YEAST

Accession

Primary (citable) accession number: P38636
Secondary accession number(s): D6W0T4, E9P8W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program