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Protein

Histidinol-phosphatase

Gene

HIS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-histidinol phosphate + H2O = L-histidinol + phosphate.

Pathwayi: L-histidine biosynthesis

This protein is involved in step 8 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (HIS1)
  2. Histidine biosynthesis trifunctional protein (HIS4)
  3. Histidine biosynthesis trifunctional protein (HIS4)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (HIS6)
  5. Imidazole glycerol phosphate synthase hisHF (HIS7)
  6. Imidazoleglycerol-phosphate dehydratase (HIS3)
  7. Histidinol-phosphate aminotransferase (HIS5)
  8. Histidinol-phosphatase (HIS2)
  9. Histidine biosynthesis trifunctional protein (HIS4)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • histidinol-phosphatase activity Source: SGD

GO - Biological processi

  • histidine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YFR025C-MONOMER.
UniPathwayiUPA00031; UER00013.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidinol-phosphatase (EC:3.1.3.15)
Short name:
HolPase
Gene namesi
Name:HIS2
Ordered Locus Names:YFR025C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR025C.
SGDiS000001921. HIS2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Histidinol-phosphatasePRO_0000122322Add
BLAST

Proteomic databases

MaxQBiP38635.

Interactioni

Protein-protein interaction databases

BioGridi31178. 6 interactions.
IntActiP38635. 2 interactions.
MINTiMINT-2780001.

Structurei

3D structure databases

ProteinModelPortaliP38635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PHP hydrolase family. HisK subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000170774.
InParanoidiP38635.
KOiK04486.
OMAiDDSHGPH.
OrthoDBiEOG092C3X96.

Family and domain databases

InterProiIPR010140. Histidinol_P_phosphatase_HisJ.
IPR004013. PHP_dom.
IPR016195. Pol/histidinol_Pase-like.
[Graphical view]
PANTHERiPTHR21039. PTHR21039. 2 hits.
PfamiPF02811. PHP. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 3 hits.
TIGRFAMsiTIGR01856. hisJ_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

P38635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSHHSHSGD YSAHGTDPLD SVVDQVVNLN FHTYCLTEHI PRIEAKFIYP
60 70 80 90 100
EEQSLGKNPE EVITKLETSF KNFMSHAQEI KTRYADRPDV RTKFIIGMEI
110 120 130 140 150
ESCDMAHIEY AKRLMKENND ILKFCVGSVH HVNGIPIDFD QQQWYNSLHS
160 170 180 190 200
FNDNLKHFLL SYFQSQYEML INIKPLVVGH FDLYKLFLPN DMLVNQKSGN
210 220 230 240 250
CNEETGVPVA SLDVISEWPE IYDAVVRNLQ FIDSYGGAIE INTSALRKRL
260 270 280 290 300
EEPYPSKTLC NLVKKHCGSR FVLSDDAHGV AQVGVCYDKV KKYIVDVLQL
310 320 330
EYICYLEESQ SPENLLTVKR LPISQFVNDP FWANI
Length:335
Mass (Da):38,582
Last modified:October 5, 2010 - v2
Checksum:i2F99522F26339DCB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301N → H in AAA18399 (PubMed:8056323).Curated
Sequence conflicti191 – 1911D → H in AAA18399 (PubMed:8056323).Curated
Sequence conflicti195 – 1951N → H in AAA18399 (PubMed:8056323).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09479 Unassigned DNA. Translation: AAA18399.1.
D50617 Genomic DNA. Translation: BAA09264.1.
BK006940 Genomic DNA. Translation: DAA12465.1.
PIRiS56280.
RefSeqiNP_116681.1. NM_001179990.1.

Genome annotation databases

EnsemblFungiiBAA09264; BAA09264; BAA09264.
YFR025C; YFR025C; YFR025C.
GeneIDi850581.
KEGGisce:YFR025C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09479 Unassigned DNA. Translation: AAA18399.1.
D50617 Genomic DNA. Translation: BAA09264.1.
BK006940 Genomic DNA. Translation: DAA12465.1.
PIRiS56280.
RefSeqiNP_116681.1. NM_001179990.1.

3D structure databases

ProteinModelPortaliP38635.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31178. 6 interactions.
IntActiP38635. 2 interactions.
MINTiMINT-2780001.

Proteomic databases

MaxQBiP38635.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09264; BAA09264; BAA09264.
YFR025C; YFR025C; YFR025C.
GeneIDi850581.
KEGGisce:YFR025C.

Organism-specific databases

EuPathDBiFungiDB:YFR025C.
SGDiS000001921. HIS2.

Phylogenomic databases

HOGENOMiHOG000170774.
InParanoidiP38635.
KOiK04486.
OMAiDDSHGPH.
OrthoDBiEOG092C3X96.

Enzyme and pathway databases

UniPathwayiUPA00031; UER00013.
BioCyciYEAST:YFR025C-MONOMER.

Miscellaneous databases

PROiP38635.

Family and domain databases

InterProiIPR010140. Histidinol_P_phosphatase_HisJ.
IPR004013. PHP_dom.
IPR016195. Pol/histidinol_Pase-like.
[Graphical view]
PANTHERiPTHR21039. PTHR21039. 2 hits.
PfamiPF02811. PHP. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 3 hits.
TIGRFAMsiTIGR01856. hisJ_fam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHIS9_YEAST
AccessioniPrimary (citable) accession number: P38635
Secondary accession number(s): D6VTQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 5, 2010
Last modified: September 7, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.