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P38634

- SIC1_YEAST

UniProt

P38634 - SIC1_YEAST

Protein

Protein SIC1

Gene

SIC1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Substrate and inhibitor of the cyclin-dependent protein kinase CDC28. Its activity could be important for faithful segregation of chromosomes to daughter cells. It acts in response to a signal from a post-start checkpoint.1 Publication

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. G1/S transition of mitotic cell cycle Source: SGD
    2. negative regulation of macroautophagy Source: SGD
    3. negative regulation of protein serine/threonine kinase activity Source: GOC
    4. regulation of cyclin-dependent protein serine/threonine kinase activity Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32230-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein SIC1
    Alternative name(s):
    CDK inhibitor p40
    Gene namesi
    Name:SIC1
    Synonyms:SDB25
    Ordered Locus Names:YLR079W
    ORF Names:L9449.8
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR079w.
    SGDiS000004069. SIC1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi173 – 1731T → A: Impairs the ability to arrest the cell cycle. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284Protein SIC1PRO_0000097751Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei5 – 51Phosphothreonine; by PHO851 Publication
    Modified residuei33 – 331Phosphothreonine1 Publication
    Modified residuei76 – 761Phosphoserine1 Publication
    Modified residuei173 – 1731Phosphothreonine2 Publications
    Modified residuei198 – 1981Phosphoserine2 Publications
    Modified residuei201 – 2011Phosphoserine2 Publications
    Modified residuei268 – 2681Lysine derivative
    Modified residuei272 – 2721Lysine derivative
    Modified residuei274 – 2741Lysine derivative

    Post-translational modificationi

    Phosphorylated by cyclin-dependent kinases CDC28 and PHO85 in association with G1-cyclins, promoting degradation of SIC1 and exit form G1.5 Publications
    May contain a covalently attached chromophore.
    The N-terminus is blocked.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38634.
    PaxDbiP38634.
    PRIDEiP38634.

    Expressioni

    Gene expression databases

    GenevestigatoriP38634.

    Interactioni

    Subunit structurei

    Interacts with HOG1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CDC14Q006842EBI-17127,EBI-4192
    HOG1P324854EBI-17127,EBI-8437

    Protein-protein interaction databases

    BioGridi31352. 314 interactions.
    DIPiDIP-6817N.
    IntActiP38634. 20 interactions.
    MINTiMINT-648606.
    STRINGi4932.YLR079W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3V7DX-ray2.31E67-85[»]
    DisProtiDP00631.
    ProteinModelPortaliP38634.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi124 – 13512Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi200 – 2067Asp/Glu-rich (acidic)

    Phylogenomic databases

    eggNOGiNOG42466.
    HOGENOMiHOG000141931.
    KOiK03085.
    OMAiINDEYAV.
    OrthoDBiEOG79GTHN.

    Sequencei

    Sequence statusi: Complete.

    P38634-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPSTPPRSR GTRYLAQPSG NTSSSALMQG QKTPQKPSQN LVPVTPSTTK    50
    SFKNAPLLAP PNSNMGMTSP FNGLTSPQRS PFPKSSVKRT LFQFESHDNG 100
    TVREEQEPLG RVNRILFPTQ QNVDIDAAEE EEEGEVLLPP SRPTSARQLH 150
    LSLERDEFDQ THRKKIIKDV PGTPSDKVIT FELAKNWNNN SPKNDARSQE 200
    SEDEEDIIIN PVRVGKNPFA SDELVTQEIR NERKRAMLRE NPDIEDVITY 250
    VNKKGEVVEK RRLTDEEKRR FKPKALFQSR DQEH 284
    Length:284
    Mass (Da):32,223
    Last modified:October 1, 1994 - v1
    Checksum:i7913636340678F78
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti167 – 1671I → T in CAA55118. (PubMed:7958845)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01300 Genomic DNA. Translation: AAA20052.1.
    X78309 Genomic DNA. Translation: CAA55118.1.
    Z73251 Genomic DNA. Translation: CAA97638.1.
    U53880 Genomic DNA. Translation: AAB67583.1.
    BK006945 Genomic DNA. Translation: DAA09395.1.
    PIRiS47921.
    RefSeqiNP_013180.1. NM_001181966.1.

    Genome annotation databases

    EnsemblFungiiYLR079W; YLR079W; YLR079W.
    GeneIDi850768.
    KEGGisce:YLR079W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01300 Genomic DNA. Translation: AAA20052.1 .
    X78309 Genomic DNA. Translation: CAA55118.1 .
    Z73251 Genomic DNA. Translation: CAA97638.1 .
    U53880 Genomic DNA. Translation: AAB67583.1 .
    BK006945 Genomic DNA. Translation: DAA09395.1 .
    PIRi S47921.
    RefSeqi NP_013180.1. NM_001181966.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3V7D X-ray 2.31 E 67-85 [» ]
    DisProti DP00631.
    ProteinModelPortali P38634.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31352. 314 interactions.
    DIPi DIP-6817N.
    IntActi P38634. 20 interactions.
    MINTi MINT-648606.
    STRINGi 4932.YLR079W.

    Proteomic databases

    MaxQBi P38634.
    PaxDbi P38634.
    PRIDEi P38634.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR079W ; YLR079W ; YLR079W .
    GeneIDi 850768.
    KEGGi sce:YLR079W.

    Organism-specific databases

    CYGDi YLR079w.
    SGDi S000004069. SIC1.

    Phylogenomic databases

    eggNOGi NOG42466.
    HOGENOMi HOG000141931.
    KOi K03085.
    OMAi INDEYAV.
    OrthoDBi EOG79GTHN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32230-MONOMER.

    Miscellaneous databases

    NextBioi 966930.

    Gene expression databases

    Genevestigatori P38634.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "An inhibitor of yeast cyclin-dependent protein kinase plays an important role in ensuring the genomic integrity of daughter cells."
      Nugroho T.T., Mendenhall M.D.
      Mol. Cell. Biol. 14:3320-3328(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 182-188; 238-250 AND 260-283.
      Strain: ATCC 204508 / S288c.
    2. "P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae."
      Donovan J.D., Toyn J.H., Johnson A.L., Johnston L.H.
      Genes Dev. 8:1640-1653(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Phosphorylation of Sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation."
      Nishizawa M., Kawasumi M., Fujino M., Toh-e A.
      Mol. Biol. Cell 9:2393-2405(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-5; THR-33 AND SER-76.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1."
      Escote X., Zapater M., Clotet J., Posas F.
      Nat. Cell Biol. 6:997-1002(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT THR-173, MUTAGENESIS OF THR-173, INTERACTION WITH HOG1.
    9. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-173 AND SER-201, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198 AND SER-201, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSIC1_YEAST
    AccessioniPrimary (citable) accession number: P38634
    Secondary accession number(s): D6VY79
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 768 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3