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P38634 (SIC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein SIC1
Alternative name(s):
CDK inhibitor p40
Gene names
Name:SIC1
Synonyms:SDB25
Ordered Locus Names:YLR079W
ORF Names:L9449.8
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length284 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate and inhibitor of the cyclin-dependent protein kinase CDC28. Its activity could be important for faithful segregation of chromosomes to daughter cells. It acts in response to a signal from a post-start checkpoint. Ref.8

Subunit structure

Interacts with HOG1. Ref.8

Subcellular location

Cytoplasm. Nucleus Ref.6.

Post-translational modification

Phosphorylated by cyclin-dependent kinases CDC28 and PHO85 in association with G1-cyclins, promoting degradation of SIC1 and exit form G1. Ref.5 Ref.8

May contain a covalently attached chromophore.

The N-terminus is blocked.

Miscellaneous

Present with 768 molecules/cell in log phase SD medium.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CDC14Q006842EBI-17127,EBI-4192
HOG1P324854EBI-17127,EBI-8437

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 284284Protein SIC1
PRO_0000097751

Regions

Compositional bias124 – 13512Asp/Glu-rich (acidic)
Compositional bias200 – 2067Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue51Phosphothreonine; by PHO85 Ref.5
Modified residue331Phosphothreonine Ref.5
Modified residue761Phosphoserine Ref.5
Modified residue1731Phosphothreonine Ref.8 Ref.10
Modified residue1981Phosphoserine Ref.9 Ref.12
Modified residue2011Phosphoserine Ref.10 Ref.12
Modified residue2681Lysine derivative
Modified residue2721Lysine derivative
Modified residue2741Lysine derivative

Experimental info

Mutagenesis1731T → A: Impairs the ability to arrest the cell cycle. Ref.8
Sequence conflict1671I → T in CAA55118. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P38634 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 7913636340678F78

FASTA28432,223
        10         20         30         40         50         60 
MTPSTPPRSR GTRYLAQPSG NTSSSALMQG QKTPQKPSQN LVPVTPSTTK SFKNAPLLAP 

        70         80         90        100        110        120 
PNSNMGMTSP FNGLTSPQRS PFPKSSVKRT LFQFESHDNG TVREEQEPLG RVNRILFPTQ 

       130        140        150        160        170        180 
QNVDIDAAEE EEEGEVLLPP SRPTSARQLH LSLERDEFDQ THRKKIIKDV PGTPSDKVIT 

       190        200        210        220        230        240 
FELAKNWNNN SPKNDARSQE SEDEEDIIIN PVRVGKNPFA SDELVTQEIR NERKRAMLRE 

       250        260        270        280 
NPDIEDVITY VNKKGEVVEK RRLTDEEKRR FKPKALFQSR DQEH 

« Hide

References

« Hide 'large scale' references
[1]"An inhibitor of yeast cyclin-dependent protein kinase plays an important role in ensuring the genomic integrity of daughter cells."
Nugroho T.T., Mendenhall M.D.
Mol. Cell. Biol. 14:3320-3328(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 182-188; 238-250 AND 260-283.
Strain: ATCC 204508 / S288c.
[2]"P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae."
Donovan J.D., Toyn J.H., Johnson A.L., Johnston L.H.
Genes Dev. 8:1640-1653(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Phosphorylation of Sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation."
Nishizawa M., Kawasumi M., Fujino M., Toh-e A.
Mol. Biol. Cell 9:2393-2405(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-5; THR-33 AND SER-76.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1."
Escote X., Zapater M., Clotet J., Posas F.
Nat. Cell Biol. 6:997-1002(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT THR-173, MUTAGENESIS OF THR-173, INTERACTION WITH HOG1.
[9]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-173 AND SER-201, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-198 AND SER-201, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U01300 Genomic DNA. Translation: AAA20052.1.
X78309 Genomic DNA. Translation: CAA55118.1.
Z73251 Genomic DNA. Translation: CAA97638.1.
U53880 Genomic DNA. Translation: AAB67583.1.
BK006945 Genomic DNA. Translation: DAA09395.1.
PIRS47921.
RefSeqNP_013180.1. NM_001181966.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3V7DX-ray2.31E67-85[»]
DisProtDP00631.
ProteinModelPortalP38634.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31352. 314 interactions.
DIPDIP-6817N.
IntActP38634. 20 interactions.
MINTMINT-648606.
STRING4932.YLR079W.

Proteomic databases

MaxQBP38634.
PaxDbP38634.
PRIDEP38634.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR079W; YLR079W; YLR079W.
GeneID850768.
KEGGsce:YLR079W.

Organism-specific databases

CYGDYLR079w.
SGDS000004069. SIC1.

Phylogenomic databases

eggNOGNOG42466.
HOGENOMHOG000141931.
KOK03085.
OMAINDEYAV.
OrthoDBEOG79GTHN.

Enzyme and pathway databases

BioCycYEAST:G3O-32230-MONOMER.

Gene expression databases

GenevestigatorP38634.

Family and domain databases

ProtoNetSearch...

Other

NextBio966930.

Entry information

Entry nameSIC1_YEAST
AccessionPrimary (citable) accession number: P38634
Secondary accession number(s): D6VY79
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references