Protein SIC1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38634 (SIC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein SIC1

Alternative name(s):
CDK inhibitor p40

Gene names

Name:	SIC1
Synonyms:	SDB25
Ordered Locus Names:	YLR079W
ORF Names:	L9449.8

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Substrate and inhibitor of the cyclin-dependent protein kinase CDC28. Its activity could be important for faithful segregation of chromosomes to daughter cells. It acts in response to a signal from a post-start checkpoint. Ref.8
Subunit structure	Interacts with HOG1. Ref.8
Subcellular location	Cytoplasm. Nucleus Ref.6.
Post-translational modification	Phosphorylated by cyclin-dependent kinases CDC28 and PHO85 in association with G1-cyclins, promoting degradation of SIC1 and exit form G1. Ref.5 Ref.8 May contain a covalently attached chromophore. The N-terminus is blocked.
Miscellaneous	Present with 768 molecules/cell in log phase SD medium.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	G1/S transition of mitotic cell cycle Inferred from genetic interaction PubMed 7954792. Source: SGD negative regulation of macroautophagy Inferred from mutant phenotype PubMed 20417603. Source: SGD regulation of cyclin-dependent protein serine/threonine kinase activity Inferred from mutant phenotype PubMed 7954792. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11792824. Source: SGD nucleus Inferred from direct assay PubMed 11080155 PubMed 11792824. Source: SGD
Molecular_function	cyclin-dependent protein serine/threonine kinase inhibitor activity Inferred from direct assay PubMed 7954792 PubMed 8421781. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	2	EBI-17127,EBI-4192
HOG1	P32485	4	EBI-17127,EBI-8437

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 284

284

Protein SIC1

PRO_0000097751

Regions

Compositional bias

124 – 135

Asp/Glu-rich (acidic)

Compositional bias

200 – 206

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

Phosphothreonine; by PHO85 Ref.5 Ref.11

Modified residue

Phosphothreonine Ref.5

Modified residue

Phosphoserine Ref.5

Modified residue

173

Phosphothreonine Ref.8 Ref.9 Ref.10 Ref.11

Modified residue

191

Phosphoserine Ref.11

Modified residue

198

Phosphoserine Ref.9

Modified residue

201

Phosphoserine Ref.10 Ref.11

Modified residue

268

Lysine derivative

Modified residue

272

Lysine derivative

Modified residue

274

Lysine derivative

Experimental info

Mutagenesis

173

T → A: Impairs the ability to arrest the cell cycle. Ref.8

Sequence conflict

167

I → T in CAA55118. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P38634 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 7913636340678F78
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FASTA

284

32,223

        10         20         30         40         50         60 
MTPSTPPRSR GTRYLAQPSG NTSSSALMQG QKTPQKPSQN LVPVTPSTTK SFKNAPLLAP 

        70         80         90        100        110        120 
PNSNMGMTSP FNGLTSPQRS PFPKSSVKRT LFQFESHDNG TVREEQEPLG RVNRILFPTQ 

       130        140        150        160        170        180 
QNVDIDAAEE EEEGEVLLPP SRPTSARQLH LSLERDEFDQ THRKKIIKDV PGTPSDKVIT 

       190        200        210        220        230        240 
FELAKNWNNN SPKNDARSQE SEDEEDIIIN PVRVGKNPFA SDELVTQEIR NERKRAMLRE 

       250        260        270        280 
NPDIEDVITY VNKKGEVVEK RRLTDEEKRR FKPKALFQSR DQEH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An inhibitor of yeast cyclin-dependent protein kinase plays an important role in ensuring the genomic integrity of daughter cells." Nugroho T.T., Mendenhall M.D. Mol. Cell. Biol. 14:3320-3328(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 182-188; 238-250 AND 260-283. Strain: ATCC 204508 / S288c.
[2]	"P40SDB25, a putative CDK inhibitor, has a role in the M/G1 transition in Saccharomyces cerevisiae." Donovan J.D., Toyn J.H., Johnson A.L., Johnston L.H. Genes Dev. 8:1640-1653(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Phosphorylation of Sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation." Nishizawa M., Kawasumi M., Fujino M., Toh-e A. Mol. Biol. Cell 9:2393-2405(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-5; THR-33 AND SER-76.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Hog1 mediates cell-cycle arrest in G1 phase by the dual targeting of Sic1." Escote X., Zapater M., Clotet J., Posas F. Nat. Cell Biol. 6:997-1002(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT THR-173, MUTAGENESIS OF THR-173, INTERACTION WITH HOG1.
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-173 AND SER-198, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-173 AND SER-201, MASS SPECTROMETRY. Strain: ADR376.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-5; THR-173; SER-191 AND SER-201, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U01300 Genomic DNA. Translation: AAA20052.1.
X78309 Genomic DNA. Translation: CAA55118.1.
Z73251 Genomic DNA. Translation: CAA97638.1.
U53880 Genomic DNA. Translation: AAB67583.1.
BK006945 Genomic DNA. Translation: DAA09395.1.

PIR

S47921.

RefSeq

NP_013180.1. NM_001181966.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3V7D	X-ray	2.31	E	67-85	[»]

ProteinModelPortal

P38634.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6817N.

IntAct

P38634. 20 interactions.

MINT

MINT-648606.

STRING

4932.YLR079W.

Proteomic databases

PaxDb

P38634.

PRIDE

P38634.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YLR079W; YLR079W; YLR079W.

GeneID

850768.

KEGG

sce:YLR079W.

Organism-specific databases

CYGD

YLR079w.

SGD

S000004069. SIC1.

Phylogenomic databases

eggNOG

NOG42466.

HOGENOM

HOG000141931.

K03085.

OMA

NPELEST.

OrthoDB

EOG47Q1GN.

Gene expression databases

Genevestigator

P38634.

GermOnline

YLR079W. Saccharomyces cerevisiae.

Family and domain databases

ProtoNet

Search...

Other

NextBio

966930.

Entry information

Entry name

SIC1_YEAST

Accession

Primary (citable) accession number: P38634
Secondary accession number(s): D6VY79

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents