E3 SUMO-protein ligase MMS21 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38632 (NSE2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
E3 SUMO-protein ligase MMS21
EC=6.3.2.-

Alternative name(s):
Methyl methanesulfonate-sensitivity protein 21
Non-structural maintenance of chromosome element 2
Short name=Non-SMC element 2

Gene names

Name:	MMS21
Synonyms:	NSE2
Ordered Locus Names:	YEL019C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as an E3 ligase mediating SUMO/Smt3 attachment to SMC5 and YKU70. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. Ref.7
Pathway	Protein modification; protein sumoylation.
Subunit structure	Component of the Smc5-Smc6 complex which consists of KRE29, NSE1, NSE2/MMS21, NSE3, NSE4, NSE5, SMC5 and SMC6. Ref.7
Subcellular location	Nucleus. Cytoplasm Ref.5 Ref.7.
Miscellaneous	Present with 2800 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the NSE2 family. Contains 1 SP-RING-type zinc finger.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	Smc5-Smc6 complex Inferred from direct assay Ref.7. Source: SGD cytoplasm Inferred from direct assay Ref.5. Source: SGD nuclear envelope Inferred from direct assay Ref.7. Source: SGD
Molecular function	SUMO ligase activity Inferred from mutant phenotype. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 267

267

E3 SUMO-protein ligase MMS21

PRO_0000218991

Regions

Zinc finger

169 – 246

SP-RING-type

Experimental info

Sequence conflict

124

E → D in AAA20471. Ref.1

Secondary structure

267

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P38632 [UniParc].

Last modified September 27, 2004. Version 2.
Checksum: F200378289B1CEE4
Show »

FASTA

267

30,354

        10         20         30         40         50         60 
MALNDNPIPK SVPLHPKSGK YFHNLHARDL SNIYQQCYKQ IDETINQLVD STSPSTIGIE 

        70         80         90        100        110        120 
EQVADITSTY KLLSTYESES NSFDEHIKDL KKNFKQSSDA CPQIDLSTWD KYRTGELTAP 

       130        140        150        160        170        180 
KLSELYLNMP TPEPATMVNN TDTLKILKVL PYIWNDPTCV IPDLQNPADE DDLQIEGGKI 

       190        200        210        220        230        240 
ELTCPITCKP YEAPLISRKC NHVFDRDGIQ NYLQGYTTRD CPQAACSQVV SMRDFVRDPI 

       250        260 
MELRCKIAKM KESQEQDKRS SQAIDVL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of the MMS21 gene of yeast." Williams T.L., Montelone B.A. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D7.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization." Zhao X., Blobel G. Proc. Natl. Acad. Sci. U.S.A. 102:4777-4782(2005) [PubMed: 15738391] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U12311 Genomic DNA. Translation: AAA20471.1.
U18530 Genomic DNA. Translation: AAB64496.1.
AY558441 Genomic DNA. Translation: AAS56767.1.
BK006939 Genomic DNA. Translation: DAA07634.1.

PIR

S50440.

RefSeq

NP_010896.1. NM_001178834.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HTK	X-ray	2.31	C	1-267	[»]

ProteinModelPortal

P38632.

SMR

P38632. Positions 5-258.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4100N.

IntAct

P38632. 3 interactions.

MINT

MINT-535631.

STRING

P38632.

Proteomic databases

PeptideAtlas

P38632.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YEL019C; YEL019C; YEL019C.

GeneID

856695.

KEGG

sce:YEL019C.

NMPDR

fig|4932.3.peg.1951.

Organism-specific databases

CYGD

YEL019c.

SGD

S000000745. MMS21.

Phylogenomic databases

eggNOG

fuNOG13380.

OMA

CPITCKP.

OrthoDB

EOG4G7G83.

Gene expression databases

ArrayExpress

P38632.

Genevestigator

P38632.

GermOnline

YEL019C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR004181. Znf_MIZ.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]

Gene3D

G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.

PROSITE

PS51044. ZF_SP_RING. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

982748.

Entry information

Entry name

NSE2_YEAST

Accession

Primary (citable) accession number: P38632
Secondary accession number(s): D3DLN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	September 27, 2004
Last modified:	December 14, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents