Replication factor C subunit 3 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P38629 (RFC3_YEAST)

Last modified December 15, 2009. Version 83. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Replication factor C subunit 3
      Short name=Replication factor C3
Alternative name(s):
    Activator 1 40 kDa subunit

Gene names

Name:	RFC3
Ordered Locus Names:	YNL290W
ORF Names:	N0533

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair. RFC3 supplies a catalytic site to the ATP site of RFC4. Ref.7 Ref.8 Ref.11 Ref.12
Subunit structure	Replication factor C (RFC) is a heteropentamer of subunits RFC1, RFC2, RFC3, RFC4 and RFC5 and forms a complex with POL30/PCNA in the presence of ATP. Component of the RAD24-RFC complex which consists of RAD14, RFC2, RFC3, RFC4 and RFC5 and associates with the checkpoint clamp DDC1:MEC3:RAD17 complex. Component of the ELG1-RFC complex which consists of ELG1, RFC2, RFC3, RFC4 and RFC5. Component of the CTF18-RFC complex, which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. RFC3 interacts with ECO1 and POL30/PCNA. Ref.7 Ref.8 Ref.5 Ref.9
Subcellular location	Nucleus Probable.
Miscellaneous	Present with 3140 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Belongs to the activator 1 small subunits family.

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Ontologies

Keywords
Biological process	Cell cycle DNA replication
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	leading strand elongation Inferred from direct assay. Source: SGD mismatch repair Traceable author statement. Source: SGD sister chromatid cohesion Ref.6 Inferred from physical interaction. Source: SGD
Cellular component	Ctf18 RFC-like complex Ref.6 Inferred from physical interaction. Source: SGD DNA replication factor C complex Ref.2 Inferred from direct assay. Source: SGD Elg1 RFC-like complex Inferred from physical interaction. Source: SGD Rad17 RFC-like complex Ref.11 Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA clamp loader activity Ref.11 Inferred from electronic annotation. Source: InterPro protein binding Ref.6 Ref.7 Ref.9 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
CTF18	P49956	1	EBI-15000,EBI-4560
CTF8	P38877	1	EBI-15000,EBI-5216
ECO1	P43605	1	EBI-15000,EBI-22988
ELG1	Q12050	1	EBI-15000,EBI-32195
RFC2	P40348	1	EBI-15000,EBI-14992
RFC4	P40339	1	EBI-15000,EBI-15009

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 340

340

Replication factor C subunit 3

PRO_0000121756

Regions

Nucleotide binding

16 – 19

ATP

Nucleotide binding

53 – 61

ATP

Sites

Binding site

ATP

Binding site

ATP; via amide nitrogen and carbonyl oxygen

Binding site

148

ATP

Binding site

206

ATP

Secondary structure

340

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P38629-1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 0170EDABAB5CEA52
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FASTA

340

38,204

        10         20         30         40         50         60 
MSTSTEKRSK ENLPWVEKYR PETLDEVYGQ NEVITTVRKF VDEGKLPHLL FYGPPGTGKT 

        70         80         90        100        110        120 
STIVALAREI YGKNYSNMVL ELNASDDRGI DVVRNQIKDF ASTRQIFSKG FKLIILDEAD 

       130        140        150        160        170        180 
AMTNAAQNAL RRVIERYTKN TRFCVLANYA HKLTPALLSR CTRFRFQPLP QEAIERRIAN 

       190        200        210        220        230        240 
VLVHEKLKLS PNAEKALIEL SNGDMRRVLN VLQSCKATLD NPDEDEISDD VIYECCGAPR 

       250        260        270        280        290        300 
PSDLKAVLKS ILEDDWGTAH YTLNKVRSAK GLALIDLIEG IVKILEDYEL QNEETRVHLL 

       310        320        330        340 
TKLADIEYSI SKGGNDQIQG SAVIGAIKAS FENETVKANV

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of the Saccharomyces cerevisiae RFC3 gene, an essential component of replication factor C." Li X., Burgers P.M.J. Proc. Natl. Acad. Sci. U.S.A. 91:868-872(1994) [PubMed: 8302859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of the five replication factor C genes of Saccharomyces cerevisiae." Cullmann G., Fien K., Kobayashi R., Stillman B. Mol. Cell. Biol. 15:4661-4671(1995) [PubMed: 7651383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION IN THE RFC COMPLEX. Strain: ATCC 204508 / S288c.
[3]	"Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV carrying a ribosomal protein gene cluster, the genes encoding a plasma membrane protein and a subunit of replication factor C, and a novel putative serine/threonine protein kinase gene." Maurer K.C.T., Urbanus J.H.M., Planta R.J. Yeast 11:1303-1310(1995) [PubMed: 8553702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"A novel Rad24 checkpoint protein complex closely related to replication factor C." Green C.M., Erdjument-Bromage H., Tempst P., Lowndes N.F. Curr. Biol. 10:39-42(2000) [PubMed: 10660302] [Abstract] Cited for: INTERACTION WITH RAD24, IDENTIFICATION IN THE RAD24-RFC COMPLEX.
[6]	"Identification of RFC(Ctf18p, Ctf8p, Dcc1p): an alternative RFC complex required for sister chromatid cohesion in S. cerevisiae." Mayer M.L., Gygi S.P., Aebersold R., Hieter P. Mol. Cell 7:959-970(2001) [PubMed: 11389843] [Abstract] Cited for: IDENTIFICATION IN THE CTF18-RFC COMPLEX.
[7]	"Chl12 (Ctf18) forms a novel replication factor C-related complex and functions redundantly with Rad24 in the DNA replication checkpoint pathway." Naiki T., Kondo T., Nakada D., Matsumoto K., Sugimoto K. Mol. Cell. Biol. 21:5838-5845(2001) [PubMed: 11486023] [Abstract] Cited for: FUNCTION, INTERACTION WITH CTF18.
[8]	"Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen." Yao N., Coryell L., Zhang D., Georgescu R.E., Finkelstein J., Coman M.M., Hingorani M.M., O'Donnell M. J. Biol. Chem. 278:50744-50753(2003) [PubMed: 14530260] [Abstract] Cited for: FUNCTION, INTERACTION WITH POL30.
[9]	"Mechanical link between cohesion establishment and DNA replication: Ctf7p/Eco1p, a cohesion establishment factor, associates with three different replication factor C complexes." Kenna M.A., Skibbens R.V. Mol. Cell. Biol. 23:2999-3007(2003) [PubMed: 12665596] [Abstract] Cited for: INTERACTION WITH ECO1.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Yeast Rad17/Mec3/Ddc1: a sliding clamp for the DNA damage checkpoint." Majka J., Burgers P.M.J. Proc. Natl. Acad. Sci. U.S.A. 100:2249-2254(2003) [PubMed: 12604797] [Abstract] Cited for: IDENTIFICATION IN THE RAD24-RFC COMPLEX, FUNCTION OF THE RAD24-RFC COMPLEX.
[12]	"Replication protein A-directed unloading of PCNA by the Ctf18 cohesion establishment complex." Bylund G.O., Burgers P.M. Mol. Cell. Biol. 25:5445-5455(2005) [PubMed: 15964801] [Abstract] Cited for: IDENTIFICATION IN THE RFC COMPLEX, IDENTIFICATION IN THE RAD24-RFC COMPLEX, IDENTIFICATION IN THE ELG1-RFC COMPLEX, IDENTIFICATION IN THE CTF18-RFC COMPLEX, FUNCTION OF THE CTF18-RFC COMPLEX.
[13]	"Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex." Bowman G.D., O'Donnell M., Kuriyan J. Nature 429:724-730(2004) [PubMed: 15201901] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) IN COMPLEX WITH AN ATP ANALOG; RCF1; RCF2; RCF4; RCF5 AND PCNA.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

L18755 Genomic DNA. Translation: AAA34969.1.
U26029 Genomic DNA. Translation: AAC49062.1.
U23084 Genomic DNA. Translation: AAC49110.1.
Z71566 Genomic DNA. Translation: CAA96207.1.

PIR

A36988.

RefSeq

NP_014109.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SXJ	X-ray	2.85	C	1-340	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2529N.

IntAct

P38629. 22 interactions.

STRING

P38629.

Proteomic databases

PeptideAtlas

P38629.

PRIDE

P38629.

Genome annotation databases

Ensembl

YNL290W; YNL290W; YNL290W; Saccharomyces cerevisiae. [Genome view]

GeneID

855426.

KEGG

sce:YNL290W.

NMPDR

fig|4932.3.peg.5171.

Organism-specific databases

CYGD

YNL290w.

SGD

S000005234. RFC3.

Phylogenomic databases

HOGENOM

HBG541599.

OMA

ENLSTGC.

OrthoDB

EOG9T4FC6.

Gene expression databases

ArrayExpress

P38629.

Genevestigator

P38629.

GermOnline

YNL290W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR013748. Replication_factor_C.
[Graphical view]

Pfam

PF00004. AAA. 1 hit.
PF08542. Rep_fac_C. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

979295.

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Entry information

Entry name

RFC3_YEAST

Accession

Primary (citable) accession number: P38629

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 15, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents