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P38628

- AGM1_YEAST

UniProt

P38628 - AGM1_YEAST

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Protein

Phosphoacetylglucosamine mutase

Gene

PCM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Interconverts GlcNAc-6-P and GlcNAc-1-P.

Catalytic activityi

N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate.

Cofactori

Binds 1 magnesium ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Phosphoserine intermediate
Metal bindingi67 – 671Magnesium; via phosphate groupBy similarity
Metal bindingi298 – 2981MagnesiumBy similarity
Metal bindingi300 – 3001MagnesiumBy similarity
Metal bindingi302 – 3021MagnesiumBy similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. phosphoacetylglucosamine mutase activity Source: SGD

GO - Biological processi

  1. fungal-type cell wall chitin biosynthetic process Source: SGD
  2. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13178.
YEAST:YEL058W-MONOMER.
ReactomeiREACT_189213. Synthesis of UDP-N-acetyl-glucosamine.
UniPathwayiUPA00113; UER00530.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoacetylglucosamine mutase (EC:5.4.2.3)
Short name:
PAGM
Alternative name(s):
Acetylglucosamine phosphomutase
N-acetylglucosamine-phosphate mutase
Gene namesi
Name:PCM1
Synonyms:AGM1
Ordered Locus Names:YEL058W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

CYGDiYEL058w.
SGDiS000000784. PCM1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Phosphoacetylglucosamine mutasePRO_0000148019Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine1 Publication

Post-translational modificationi

Phosphorylation at Ser-67 is involved in the reaction mechanism.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38628.
PaxDbiP38628.
PeptideAtlasiP38628.

Expressioni

Gene expression databases

GenevestigatoriP38628.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MGS1P401512EBI-2351,EBI-29152

Protein-protein interaction databases

BioGridi36671. 15 interactions.
DIPiDIP-6760N.
IntActiP38628. 1 interaction.
MINTiMINT-637715.
STRINGi4932.YEL058W.

Structurei

3D structure databases

ProteinModelPortaliP38628.
SMRiP38628. Positions 17-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphohexose mutase family.Curated

Phylogenomic databases

eggNOGiCOG1109.
GeneTreeiENSGT00390000000509.
HOGENOMiHOG000210027.
InParanoidiP38628.
KOiK01836.
OMAiDIVRVYA.
OrthoDBiEOG7V1G0D.

Family and domain databases

Gene3Di3.30.310.50. 1 hit.
3.40.120.10. 2 hits.
InterProiIPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
IPR016657. PAGM.
[Graphical view]
PfamiPF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view]
PIRSFiPIRSF016408. PAGM. 1 hit.
SUPFAMiSSF53738. SSF53738. 3 hits.
SSF55957. SSF55957. 1 hit.
PROSITEiPS00710. PGM_PMM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38628-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVDYEQLCK LYDDTCRTKN VQFSYGTAGF RTLAKNLDTV MFSTGILAVL
60 70 80 90 100
RSLKLQGQYV GVMITASHNP YQDNGVKIVE PDGSMLLATW EPYAMQLANA
110 120 130 140 150
ASFATNFEEF RVELAKLIEH EKIDLNTTVV PHIVVGRDSR ESSPYLLRCL
160 170 180 190 200
TSSMASVFHA QVLDLGCVTT PQLHYITDLS NRRKLEGDTA PVATEQDYYS
210 220 230 240 250
FFIGAFNELF ATYQLEKRLS VPKLFIDTAN GIGGPQLKKL LASEDWDVPA
260 270 280 290 300
EQVEVINDRS DVPELLNFEC GADYVKTNQR LPKGLSPSSF DSLYCSFDGD
310 320 330 340 350
ADRVVFYYVD SGSKFHLLDG DKISTLFAKF LSKQLELAHL EHSLKIGVVQ
360 370 380 390 400
TAYANGSSTA YIKNTLHCPV SCTKTGVKHL HHEAATQYDI GIYFEANGHG
410 420 430 440 450
TIIFSEKFHR TIKSELSKSK LNGDTLALRT LKCFSELINQ TVGDAISDML
460 470 480 490 500
AVLATLAILK MSPMDWDEEY TDLPNKLVKC IVPDRSIFQT TDQERKLLNP
510 520 530 540 550
VGLQDKIDLV VAKYPMGRSF VRASGTEDAV RVYAECKDSS KLGQFCDEVV

EHVKASA
Length:557
Mass (Da):62,067
Last modified:February 1, 1995 - v2
Checksum:i76F17D47D07C920A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151T → M in CAA53452. (PubMed:8119301)Curated
Sequence conflicti196 – 1961Q → R in CAA53452. (PubMed:8119301)Curated
Sequence conflicti406 – 4061E → G in CAA53452. (PubMed:8119301)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75816 Genomic DNA. Translation: CAA53452.1.
U18795 Genomic DNA. Translation: AAB65029.1.
BK006939 Genomic DNA. Translation: DAA07596.1.
PIRiS50531.
RefSeqiNP_010856.1. NM_001178873.1.

Genome annotation databases

EnsemblFungiiYEL058W; YEL058W; YEL058W.
GeneIDi856652.
KEGGisce:YEL058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X75816 Genomic DNA. Translation: CAA53452.1 .
U18795 Genomic DNA. Translation: AAB65029.1 .
BK006939 Genomic DNA. Translation: DAA07596.1 .
PIRi S50531.
RefSeqi NP_010856.1. NM_001178873.1.

3D structure databases

ProteinModelPortali P38628.
SMRi P38628. Positions 17-554.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36671. 15 interactions.
DIPi DIP-6760N.
IntActi P38628. 1 interaction.
MINTi MINT-637715.
STRINGi 4932.YEL058W.

Proteomic databases

MaxQBi P38628.
PaxDbi P38628.
PeptideAtlasi P38628.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YEL058W ; YEL058W ; YEL058W .
GeneIDi 856652.
KEGGi sce:YEL058W.

Organism-specific databases

CYGDi YEL058w.
SGDi S000000784. PCM1.

Phylogenomic databases

eggNOGi COG1109.
GeneTreei ENSGT00390000000509.
HOGENOMi HOG000210027.
InParanoidi P38628.
KOi K01836.
OMAi DIVRVYA.
OrthoDBi EOG7V1G0D.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00530 .
BioCyci MetaCyc:MONOMER-13178.
YEAST:YEL058W-MONOMER.
Reactomei REACT_189213. Synthesis of UDP-N-acetyl-glucosamine.

Miscellaneous databases

NextBioi 982631.
PROi P38628.

Gene expression databases

Genevestigatori P38628.

Family and domain databases

Gene3Di 3.30.310.50. 1 hit.
3.40.120.10. 2 hits.
InterProi IPR005844. A-D-PHexomutase_a/b/a-I.
IPR016055. A-D-PHexomutase_a/b/a-I/II/III.
IPR005845. A-D-PHexomutase_a/b/a-II.
IPR005843. A-D-PHexomutase_C.
IPR016066. A-D-PHexomutase_CS.
IPR016657. PAGM.
[Graphical view ]
Pfami PF02878. PGM_PMM_I. 1 hit.
PF02879. PGM_PMM_II. 1 hit.
PF00408. PGM_PMM_IV. 1 hit.
[Graphical view ]
PIRSFi PIRSF016408. PAGM. 1 hit.
SUPFAMi SSF53738. SSF53738. 3 hits.
SSF55957. SSF55957. 1 hit.
PROSITEi PS00710. PGM_PMM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A family of hexosephosphate mutases in Saccharomyces cerevisiae."
    Boles E., Liebetrau W., Hofmann M., Zimmermann F.K.
    Eur. J. Biochem. 220:83-96(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: EBY21-8.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase."
    Hofmann M., Boles E., Zimmermann F.K.
    Eur. J. Biochem. 221:741-747(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: EBY21-8.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAGM1_YEAST
AccessioniPrimary (citable) accession number: P38628
Secondary accession number(s): D3DLJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 14700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3