SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P38625

- GUAA_YEAST

UniProt

P38625 - GUAA_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

GMP synthase [glutamine-hydrolyzing]

Gene
GUA1, YMR217W, YM8261.11
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891For GATase activity By similarity
Active sitei176 – 1761For GATase activity By similarity
Active sitei178 – 1781For GATase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi231 – 2377ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GMP synthase (glutamine-hydrolyzing) activity Source: SGD
  3. pyrophosphatase activity Source: InterPro

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. GMP biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR217W-MONOMER.
YEAST:YMR217W-MONOMER.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.957.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:GUA1
Ordered Locus Names:YMR217W
ORF Names:YM8261.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004830. GUA1.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 525524GMP synthase [glutamine-hydrolyzing]UniRule annotationPRO_0000140258Add
BLAST

Proteomic databases

MaxQBiP38625.
PaxDbiP38625.
PeptideAtlasiP38625.

Expressioni

Gene expression databases

GenevestigatoriP38625.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

BioGridi35395. 12 interactions.
IntActiP38625. 5 interactions.
STRINGi4932.YMR217W.

Structurei

3D structure databases

ProteinModelPortaliP38625.
SMRiP38625. Positions 14-525.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 202190Glutamine amidotransferase type-1Add
BLAST
Domaini203 – 400198GMPS ATP-PPaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0519.
GeneTreeiENSGT00390000006591.
HOGENOMiHOG000223964.
KOiK01951.
OMAiPYNKFPH.
OrthoDBiEOG7J70QG.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38625-1 [UniParc]FASTAAdd to Basket

« Hide

MAAGEQVSNM FDTILVLDFG SQYSHLITRR LREFNIYAEM LPCTQKISEL    50
GWTPKGVILS GGPYSVYAED APHVDHAIFD LNVPILGICY GMQELAWING 100
KQVGRGDKRE YGPATLKVID DSNSLFKGMN DSTVWMSHGD KLHGLPTGYK 150
TIATSDNSPY CGIVHETKPI YGIQFHPEVT HSTQGKTLLK NFAVDLCHAK 200
QNWTMENFID TEINRIRKLV GPTAEVIGAV SGGVDSTVAS KLMTEAIGDR 250
FHAILVDNGV LRLNEAANVK KTLVEGLGIN LMVVDASEEF LSKLKGVTDP 300
EKKRKIIGNT FIHVFEREAE KIKPKDGKEI QFLLQGTLYP DVIESISFKG 350
PSQTIKTHHN VGGLLENMKL KLIEPLRELF KDEVRHLGEL LGIPHDLVWR 400
HPFPGPGIAI RVLGEVTKEQ VEIARKADNI YIEEIKKAGL YNQISQAFAC 450
LLPVKSVGVM GDQRTYDQVI ALRAIETTDF MTADWFPFEH SFLKKVASRI 500
VNEVDGVARV TYDITSKPPA TVEWE 525
Length:525
Mass (Da):58,482
Last modified:January 23, 2007 - v4
Checksum:i56509DE34A9830FA
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti325 – 3251K → R in CAA49847. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70397 Genomic DNA. Translation: CAA49847.1.
Z49809 Genomic DNA. Translation: CAA89932.1.
BK006946 Genomic DNA. Translation: DAA10116.1.
PIRiS55099.
RefSeqiNP_013944.1. NM_001182724.1.

Genome annotation databases

EnsemblFungiiYMR217W; YMR217W; YMR217W.
GeneIDi855257.
KEGGisce:YMR217W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70397 Genomic DNA. Translation: CAA49847.1 .
Z49809 Genomic DNA. Translation: CAA89932.1 .
BK006946 Genomic DNA. Translation: DAA10116.1 .
PIRi S55099.
RefSeqi NP_013944.1. NM_001182724.1.

3D structure databases

ProteinModelPortali P38625.
SMRi P38625. Positions 14-525.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35395. 12 interactions.
IntActi P38625. 5 interactions.
STRINGi 4932.YMR217W.

Protein family/group databases

MEROPSi C26.957.

Proteomic databases

MaxQBi P38625.
PaxDbi P38625.
PeptideAtlasi P38625.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR217W ; YMR217W ; YMR217W .
GeneIDi 855257.
KEGGi sce:YMR217W.

Organism-specific databases

SGDi S000004830. GUA1.

Phylogenomic databases

eggNOGi COG0519.
GeneTreei ENSGT00390000006591.
HOGENOMi HOG000223964.
KOi K01951.
OMAi PYNKFPH.
OrthoDBi EOG7J70QG.

Enzyme and pathway databases

UniPathwayi UPA00189 ; UER00296 .
BioCyci MetaCyc:YMR217W-MONOMER.
YEAST:YMR217W-MONOMER.

Miscellaneous databases

NextBioi 978843.
PROi P38625.

Gene expression databases

Genevestigatori P38625.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPi MF_00344. GMP_synthase.
InterProi IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the GMP synthetase-encoding gene of Saccharomyces cerevisiae."
    Dujardin G., Kermorgant M., Slonimski P.P., Boucherie H.
    Gene 139:127-132(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 44827 / SKQ2N.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index."
    Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M.
    Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20.
    Strain: ATCC 204508 / S288c.
  5. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGUAA_YEAST
AccessioniPrimary (citable) accession number: P38625
Secondary accession number(s): D6W042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 133 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi