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P38625 (GUAA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GMP synthase [glutamine-hydrolyzing]

EC=6.3.5.2
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene names
Name:GUA1
Ordered Locus Names:YMR217W
ORF Names:YM8261.11
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP-Rule MF_00344

Pathway

Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP-Rule MF_00344

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00344

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00344.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 525524GMP synthase [glutamine-hydrolyzing] HAMAP-Rule MF_00344
PRO_0000140258

Regions

Domain13 – 202190Glutamine amidotransferase type-1
Domain203 – 400198GMPS ATP-PPase
Nucleotide binding231 – 2377ATP By similarity

Sites

Active site891For GATase activity By similarity
Active site1761For GATase activity By similarity
Active site1781For GATase activity By similarity

Experimental info

Sequence conflict3251K → R in CAA49847. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P38625 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 56509DE34A9830FA

FASTA52558,482
        10         20         30         40         50         60 
MAAGEQVSNM FDTILVLDFG SQYSHLITRR LREFNIYAEM LPCTQKISEL GWTPKGVILS 

        70         80         90        100        110        120 
GGPYSVYAED APHVDHAIFD LNVPILGICY GMQELAWING KQVGRGDKRE YGPATLKVID 

       130        140        150        160        170        180 
DSNSLFKGMN DSTVWMSHGD KLHGLPTGYK TIATSDNSPY CGIVHETKPI YGIQFHPEVT 

       190        200        210        220        230        240 
HSTQGKTLLK NFAVDLCHAK QNWTMENFID TEINRIRKLV GPTAEVIGAV SGGVDSTVAS 

       250        260        270        280        290        300 
KLMTEAIGDR FHAILVDNGV LRLNEAANVK KTLVEGLGIN LMVVDASEEF LSKLKGVTDP 

       310        320        330        340        350        360 
EKKRKIIGNT FIHVFEREAE KIKPKDGKEI QFLLQGTLYP DVIESISFKG PSQTIKTHHN 

       370        380        390        400        410        420 
VGGLLENMKL KLIEPLRELF KDEVRHLGEL LGIPHDLVWR HPFPGPGIAI RVLGEVTKEQ 

       430        440        450        460        470        480 
VEIARKADNI YIEEIKKAGL YNQISQAFAC LLPVKSVGVM GDQRTYDQVI ALRAIETTDF 

       490        500        510        520 
MTADWFPFEH SFLKKVASRI VNEVDGVARV TYDITSKPPA TVEWE 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of the GMP synthetase-encoding gene of Saccharomyces cerevisiae."
Dujardin G., Kermorgant M., Slonimski P.P., Boucherie H.
Gene 139:127-132(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 44827 / SKQ2N.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index."
Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M.
Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-20.
Strain: ATCC 204508 / S288c.
[5]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X70397 Genomic DNA. Translation: CAA49847.1.
Z49809 Genomic DNA. Translation: CAA89932.1.
BK006946 Genomic DNA. Translation: DAA10116.1.
PIRS55099.
RefSeqNP_013944.1. NM_001182724.1.

3D structure databases

ProteinModelPortalP38625.
SMRP38625. Positions 14-525.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35395. 12 interactions.
IntActP38625. 5 interactions.
STRING4932.YMR217W.

Protein family/group databases

MEROPSC26.957.

Proteomic databases

PaxDbP38625.
PeptideAtlasP38625.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR217W; YMR217W; YMR217W.
GeneID855257.
KEGGsce:YMR217W.

Organism-specific databases

SGDS000004830. GUA1.

Phylogenomic databases

eggNOGCOG0519.
GeneTreeENSGT00390000006591.
HOGENOMHOG000223964.
KOK01951.
OMAEIKKEYC.
OrthoDBEOG7J70QG.

Enzyme and pathway databases

BioCycMetaCyc:YMR217W-MONOMER.
YEAST:YMR217W-MONOMER.
UniPathwayUPA00189; UER00296.

Gene expression databases

GenevestigatorP38625.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00344. GMP_synthase.
InterProIPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978843.
PROP38625.

Entry information

Entry nameGUAA_YEAST
AccessionPrimary (citable) accession number: P38625
Secondary accession number(s): D6W042
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways