P38625 (GUAA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	GUA1
Ordered Locus Names:	YMR217W
ORF Names:	YM8261.11

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	GMP biosynthetic process Inferred from mutant phenotype PubMed 20980241. Source: SGD glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from mutant phenotype Ref.1. Source: SGD pyrophosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 525	524	GMP synthase [glutamine-hydrolyzing]		PRO_0000140258
Regions
	Domain	13 – 202	190	Glutamine amidotransferase type-1
	Domain	203 – 400	198	GMPS ATP-PPase
	Nucleotide binding	231 – 237	7	ATP By similarity
Sites
	Active site	89	1	For GATase activity By similarity
	Active site	176	1	For GATase activity By similarity
	Active site	178	1	For GATase activity By similarity
Amino acid modifications
	Modified residue	124	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	325	1	K → R in CAA49847. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38625 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 56509DE34A9830FA	FASTA	525	58,482
	10 20 30 40 50 60 MAAGEQVSNM FDTILVLDFG SQYSHLITRR LREFNIYAEM LPCTQKISEL GWTPKGVILS 70 80 90 100 110 120 GGPYSVYAED APHVDHAIFD LNVPILGICY GMQELAWING KQVGRGDKRE YGPATLKVID 130 140 150 160 170 180 DSNSLFKGMN DSTVWMSHGD KLHGLPTGYK TIATSDNSPY CGIVHETKPI YGIQFHPEVT 190 200 210 220 230 240 HSTQGKTLLK NFAVDLCHAK QNWTMENFID TEINRIRKLV GPTAEVIGAV SGGVDSTVAS 250 260 270 280 290 300 KLMTEAIGDR FHAILVDNGV LRLNEAANVK KTLVEGLGIN LMVVDASEEF LSKLKGVTDP 310 320 330 340 350 360 EKKRKIIGNT FIHVFEREAE KIKPKDGKEI QFLLQGTLYP DVIESISFKG PSQTIKTHHN 370 380 390 400 410 420 VGGLLENMKL KLIEPLRELF KDEVRHLGEL LGIPHDLVWR HPFPGPGIAI RVLGEVTKEQ 430 440 450 460 470 480 VEIARKADNI YIEEIKKAGL YNQISQAFAC LLPVKSVGVM GDQRTYDQVI ALRAIETTDF 490 500 510 520 MTADWFPFEH SFLKKVASRI VNEVDGVARV TYDITSKPPA TVEWE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the GMP synthetase-encoding gene of Saccharomyces cerevisiae." Dujardin G., Kermorgant M., Slonimski P.P., Boucherie H. Gene 139:127-132(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 44827 / SKQ2N.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index." Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M. Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20. Strain: ATCC 204508 / S288c.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-124, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70397 Genomic DNA. Translation: CAA49847.1. Z49809 Genomic DNA. Translation: CAA89932.1. BK006946 Genomic DNA. Translation: DAA10116.1.
PIR	S55099.
RefSeq	NP_013944.1. NM_001182724.1.
3D structure databases
ProteinModelPortal	P38625.
SMR	P38625. Positions 14-525.
ModBase	Search...
Protein-protein interaction databases
IntAct	P38625. 3 interactions.
STRING	4932.YMR217W.
Protein family/group databases
MEROPS	C26.957.
Proteomic databases
PaxDb	P38625.
PeptideAtlas	P38625.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR217W; YMR217W; YMR217W.
GeneID	855257.
KEGG	sce:YMR217W.
Organism-specific databases
SGD	S000004830. GUA1.
Phylogenomic databases
eggNOG	COG0519.
GeneTree	ENSGT00390000006591.
HOGENOM	HOG000223964.
KO	K01951.
OMA	YDYVVAL.
OrthoDB	EOG4WWVST.
Enzyme and pathway databases
BioCyc	MetaCyc:YMR217W-MONOMER. YEAST:YMR217W-MONOMER.
UniPathway	UPA00189; UER00296.
Gene expression databases
Genevestigator	P38625.
GermOnline	YMR217W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR017926. GATASE. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR025777. GMPS_ATP_PPase_dom. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit. TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978843.

Entry information

Entry name

GUAA_YEAST

Accession

Primary (citable) accession number: P38625
Secondary accession number(s): D6W042

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 126 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program