Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P38625

- GUAA_YEAST

UniProt

P38625 - GUAA_YEAST

Protein

GMP synthase [glutamine-hydrolyzing]

Gene

GUA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891For GATase activityBy similarity
    Active sitei176 – 1761For GATase activityBy similarity
    Active sitei178 – 1781For GATase activityBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi231 – 2377ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. GMP synthase (glutamine-hydrolyzing) activity Source: SGD
    3. pyrophosphatase activity Source: InterPro

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. GMP biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YMR217W-MONOMER.
    YEAST:YMR217W-MONOMER.
    UniPathwayiUPA00189; UER00296.

    Protein family/group databases

    MEROPSiC26.957.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
    Alternative name(s):
    GMP synthetase
    Glutamine amidotransferase
    Gene namesi
    Name:GUA1
    Ordered Locus Names:YMR217W
    ORF Names:YM8261.11
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004830. GUA1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 525524GMP synthase [glutamine-hydrolyzing]PRO_0000140258Add
    BLAST

    Proteomic databases

    MaxQBiP38625.
    PaxDbiP38625.
    PeptideAtlasiP38625.

    Expressioni

    Gene expression databases

    GenevestigatoriP38625.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi35395. 12 interactions.
    IntActiP38625. 5 interactions.
    STRINGi4932.YMR217W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38625.
    SMRiP38625. Positions 14-525.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 202190Glutamine amidotransferase type-1Add
    BLAST
    Domaini203 – 400198GMPS ATP-PPaseAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0519.
    GeneTreeiENSGT00390000006591.
    HOGENOMiHOG000223964.
    KOiK01951.
    OMAiPYNKFPH.
    OrthoDBiEOG7J70QG.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.40.50.880. 1 hit.
    HAMAPiMF_00344. GMP_synthase.
    InterProiIPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR001674. GMP_synth_C.
    IPR004739. GMP_synth_N.
    IPR022955. GMP_synthase.
    IPR025777. GMPS_ATP_PPase_dom.
    IPR022310. NAD/GMP_synthase.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    PF00958. GMP_synt_C. 1 hit.
    PF02540. NAD_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
    TIGR00888. guaA_Nterm. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    PS51553. GMPS_ATP_PPASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38625-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAGEQVSNM FDTILVLDFG SQYSHLITRR LREFNIYAEM LPCTQKISEL    50
    GWTPKGVILS GGPYSVYAED APHVDHAIFD LNVPILGICY GMQELAWING 100
    KQVGRGDKRE YGPATLKVID DSNSLFKGMN DSTVWMSHGD KLHGLPTGYK 150
    TIATSDNSPY CGIVHETKPI YGIQFHPEVT HSTQGKTLLK NFAVDLCHAK 200
    QNWTMENFID TEINRIRKLV GPTAEVIGAV SGGVDSTVAS KLMTEAIGDR 250
    FHAILVDNGV LRLNEAANVK KTLVEGLGIN LMVVDASEEF LSKLKGVTDP 300
    EKKRKIIGNT FIHVFEREAE KIKPKDGKEI QFLLQGTLYP DVIESISFKG 350
    PSQTIKTHHN VGGLLENMKL KLIEPLRELF KDEVRHLGEL LGIPHDLVWR 400
    HPFPGPGIAI RVLGEVTKEQ VEIARKADNI YIEEIKKAGL YNQISQAFAC 450
    LLPVKSVGVM GDQRTYDQVI ALRAIETTDF MTADWFPFEH SFLKKVASRI 500
    VNEVDGVARV TYDITSKPPA TVEWE 525
    Length:525
    Mass (Da):58,482
    Last modified:January 23, 2007 - v4
    Checksum:i56509DE34A9830FA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti325 – 3251K → R in CAA49847. (PubMed:8112582)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70397 Genomic DNA. Translation: CAA49847.1.
    Z49809 Genomic DNA. Translation: CAA89932.1.
    BK006946 Genomic DNA. Translation: DAA10116.1.
    PIRiS55099.
    RefSeqiNP_013944.1. NM_001182724.1.

    Genome annotation databases

    EnsemblFungiiYMR217W; YMR217W; YMR217W.
    GeneIDi855257.
    KEGGisce:YMR217W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X70397 Genomic DNA. Translation: CAA49847.1 .
    Z49809 Genomic DNA. Translation: CAA89932.1 .
    BK006946 Genomic DNA. Translation: DAA10116.1 .
    PIRi S55099.
    RefSeqi NP_013944.1. NM_001182724.1.

    3D structure databases

    ProteinModelPortali P38625.
    SMRi P38625. Positions 14-525.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35395. 12 interactions.
    IntActi P38625. 5 interactions.
    STRINGi 4932.YMR217W.

    Protein family/group databases

    MEROPSi C26.957.

    Proteomic databases

    MaxQBi P38625.
    PaxDbi P38625.
    PeptideAtlasi P38625.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR217W ; YMR217W ; YMR217W .
    GeneIDi 855257.
    KEGGi sce:YMR217W.

    Organism-specific databases

    SGDi S000004830. GUA1.

    Phylogenomic databases

    eggNOGi COG0519.
    GeneTreei ENSGT00390000006591.
    HOGENOMi HOG000223964.
    KOi K01951.
    OMAi PYNKFPH.
    OrthoDBi EOG7J70QG.

    Enzyme and pathway databases

    UniPathwayi UPA00189 ; UER00296 .
    BioCyci MetaCyc:YMR217W-MONOMER.
    YEAST:YMR217W-MONOMER.

    Miscellaneous databases

    NextBioi 978843.
    PROi P38625.

    Gene expression databases

    Genevestigatori P38625.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.40.50.880. 1 hit.
    HAMAPi MF_00344. GMP_synthase.
    InterProi IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR001674. GMP_synth_C.
    IPR004739. GMP_synth_N.
    IPR022955. GMP_synthase.
    IPR025777. GMPS_ATP_PPase_dom.
    IPR022310. NAD/GMP_synthase.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00117. GATase. 1 hit.
    PF00958. GMP_synt_C. 1 hit.
    PF02540. NAD_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR00884. guaA_Cterm. 1 hit.
    TIGR00888. guaA_Nterm. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    PS51553. GMPS_ATP_PPASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the GMP synthetase-encoding gene of Saccharomyces cerevisiae."
      Dujardin G., Kermorgant M., Slonimski P.P., Boucherie H.
      Gene 139:127-132(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 44827 / SKQ2N.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index."
      Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M.
      Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-20.
      Strain: ATCC 204508 / S288c.
    5. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGUAA_YEAST
    AccessioniPrimary (citable) accession number: P38625
    Secondary accession number(s): D6W042
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3