Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GMP synthase [glutamine-hydrolyzing]

Gene

GUA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891For GATase activityBy similarity
Active sitei176 – 1761For GATase activityBy similarity
Active sitei178 – 1781For GATase activityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi231 – 2377ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GMP synthase (glutamine-hydrolyzing) activity Source: SGD
  3. pyrophosphatase activity Source: InterPro

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. GMP biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YMR217W-MONOMER.
YEAST:YMR217W-MONOMER.
ReactomeiREACT_301060. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.957.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Glutamine amidotransferase
Gene namesi
Name:GUA1
Ordered Locus Names:YMR217W
ORF Names:YM8261.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR217W.
SGDiS000004830. GUA1.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 525524GMP synthase [glutamine-hydrolyzing]PRO_0000140258Add
BLAST

Proteomic databases

MaxQBiP38625.
PaxDbiP38625.
PeptideAtlasiP38625.

Expressioni

Gene expression databases

GenevestigatoriP38625.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi35395. 13 interactions.
IntActiP38625. 5 interactions.
STRINGi4932.YMR217W.

Structurei

3D structure databases

ProteinModelPortaliP38625.
SMRiP38625. Positions 14-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 202190Glutamine amidotransferase type-1Add
BLAST
Domaini203 – 400198GMPS ATP-PPaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0519.
GeneTreeiENSGT00390000006591.
HOGENOMiHOG000223964.
InParanoidiP38625.
KOiK01951.
OMAiKNAKWLA.
OrthoDBiEOG7J70QG.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38625-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGEQVSNM FDTILVLDFG SQYSHLITRR LREFNIYAEM LPCTQKISEL
60 70 80 90 100
GWTPKGVILS GGPYSVYAED APHVDHAIFD LNVPILGICY GMQELAWING
110 120 130 140 150
KQVGRGDKRE YGPATLKVID DSNSLFKGMN DSTVWMSHGD KLHGLPTGYK
160 170 180 190 200
TIATSDNSPY CGIVHETKPI YGIQFHPEVT HSTQGKTLLK NFAVDLCHAK
210 220 230 240 250
QNWTMENFID TEINRIRKLV GPTAEVIGAV SGGVDSTVAS KLMTEAIGDR
260 270 280 290 300
FHAILVDNGV LRLNEAANVK KTLVEGLGIN LMVVDASEEF LSKLKGVTDP
310 320 330 340 350
EKKRKIIGNT FIHVFEREAE KIKPKDGKEI QFLLQGTLYP DVIESISFKG
360 370 380 390 400
PSQTIKTHHN VGGLLENMKL KLIEPLRELF KDEVRHLGEL LGIPHDLVWR
410 420 430 440 450
HPFPGPGIAI RVLGEVTKEQ VEIARKADNI YIEEIKKAGL YNQISQAFAC
460 470 480 490 500
LLPVKSVGVM GDQRTYDQVI ALRAIETTDF MTADWFPFEH SFLKKVASRI
510 520
VNEVDGVARV TYDITSKPPA TVEWE
Length:525
Mass (Da):58,482
Last modified:January 23, 2007 - v4
Checksum:i56509DE34A9830FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti325 – 3251K → R in CAA49847 (PubMed:8112582).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70397 Genomic DNA. Translation: CAA49847.1.
Z49809 Genomic DNA. Translation: CAA89932.1.
BK006946 Genomic DNA. Translation: DAA10116.1.
PIRiS55099.
RefSeqiNP_013944.1. NM_001182724.1.

Genome annotation databases

EnsemblFungiiYMR217W; YMR217W; YMR217W.
GeneIDi855257.
KEGGisce:YMR217W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70397 Genomic DNA. Translation: CAA49847.1.
Z49809 Genomic DNA. Translation: CAA89932.1.
BK006946 Genomic DNA. Translation: DAA10116.1.
PIRiS55099.
RefSeqiNP_013944.1. NM_001182724.1.

3D structure databases

ProteinModelPortaliP38625.
SMRiP38625. Positions 14-525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35395. 13 interactions.
IntActiP38625. 5 interactions.
STRINGi4932.YMR217W.

Protein family/group databases

MEROPSiC26.957.

Proteomic databases

MaxQBiP38625.
PaxDbiP38625.
PeptideAtlasiP38625.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR217W; YMR217W; YMR217W.
GeneIDi855257.
KEGGisce:YMR217W.

Organism-specific databases

EuPathDBiFungiDB:YMR217W.
SGDiS000004830. GUA1.

Phylogenomic databases

eggNOGiCOG0519.
GeneTreeiENSGT00390000006591.
HOGENOMiHOG000223964.
InParanoidiP38625.
KOiK01951.
OMAiKNAKWLA.
OrthoDBiEOG7J70QG.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.
BioCyciMetaCyc:YMR217W-MONOMER.
YEAST:YMR217W-MONOMER.
ReactomeiREACT_301060. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi978843.
PROiP38625.

Gene expression databases

GenevestigatoriP38625.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_N.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the GMP synthetase-encoding gene of Saccharomyces cerevisiae."
    Dujardin G., Kermorgant M., Slonimski P.P., Boucherie H.
    Gene 139:127-132(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 44827 / SKQ2N.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Two-dimensional protein map of Saccharomyces cerevisiae: construction of a gene-protein index."
    Boucherie H., Dujardin G., Kermorgant M., Monribot C., Slonimski P.P., Perrot M.
    Yeast 11:601-613(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20.
    Strain: ATCC 204508 / S288c.
  5. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGUAA_YEAST
AccessioniPrimary (citable) accession number: P38625
Secondary accession number(s): D6W042
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 139 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.