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Protein

Ribose-phosphate pyrophosphokinase 2

Gene

PRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).2 Publications

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.2 Publications

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase 2 (PRS2), Ribose-phosphate pyrophosphokinase 4 (PRS4), Ribose-phosphate pyrophosphokinase 1 (PRS1), Ribose-phosphate pyrophosphokinase 5 (PRS5), Ribose-phosphate pyrophosphokinase 3 (PRS3)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi132 – 1321MagnesiumSequence analysis
Metal bindingi134 – 1341MagnesiumSequence analysis
Metal bindingi143 – 1431MagnesiumSequence analysis
Metal bindingi147 – 1471MagnesiumSequence analysis

GO - Molecular functioni

GO - Biological processi

  • 5-phosphoribose 1-diphosphate biosynthetic process Source: SGD
  • fungal-type cell wall organization Source: SGD
  • nucleotide biosynthetic process Source: UniProtKB-KW
  • ribonucleoside monophosphate biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YER099C-MONOMER.
ReactomeiR-SCE-73843. 5-Phosphoribose 1-diphosphate biosynthesis.
UniPathwayiUPA00087; UER00172.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinase 2 (EC:2.7.6.1)
Alternative name(s):
Phosphoribosyl pyrophosphate synthase 2
Gene namesi
Name:PRS2
Synonyms:PRPS2, PRS
Ordered Locus Names:YER099C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER099C.
SGDiS000000901. PRS2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • ribose phosphate diphosphokinase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Ribose-phosphate pyrophosphokinase 2PRO_0000141087Add
BLAST

Proteomic databases

MaxQBiP38620.

PTM databases

iPTMnetiP38620.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PRS1P328953EBI-9873,EBI-9869
PRS5Q122654EBI-9873,EBI-9886

Protein-protein interaction databases

BioGridi36845. 15 interactions.
DIPiDIP-1482N.
IntActiP38620. 4 interactions.
MINTiMINT-404277.

Structurei

3D structure databases

ProteinModelPortaliP38620.
SMRiP38620. Positions 5-317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074583.
HOGENOMiHOG000210451.
InParanoidiP38620.
KOiK00948.
OMAiMENGANS.
OrthoDBiEOG78M0B7.

Family and domain databases

Gene3Di3.40.50.2020. 2 hits.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38620-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNSIKLLA GNSHPGLAEL ISQRLGVPLS KVGVYQYSNK ETSVTIGESI
60 70 80 90 100
RDEDVYIIQT GYGEHEINDF LMELLILIHA CKTASVRRIT AVIPNFPYAR
110 120 130 140 150
QDKKDKSRAP ITAKLIANLL ETAGCDHVIT MDLHASQIQG FFHIPVDNLY
160 170 180 190 200
GEPSVLNYIR TKTDFNNAIL VSPDAGGAKR VASLADKLDM NFALIHKERQ
210 220 230 240 250
KANEVSRMLL VGDVAGKSCL LIDDMADTCG TLVKACDTLM DHGAKEVIAI
260 270 280 290 300
VTHGIFSGSA REKLINSRLS RIVCTNTVPV DLDLDIVDQV DISPTIAEAI
310
RRLHNGESVS YLFTHAPV
Length:318
Mass (Da):34,765
Last modified:October 1, 1994 - v1
Checksum:i8B970E98084F5D71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74414 Genomic DNA. Translation: CAA52436.1.
X75075 Genomic DNA. Translation: CAA52969.1.
U18839 Genomic DNA. Translation: AAB64654.1.
AY692977 Genomic DNA. Translation: AAT92996.1.
BK006939 Genomic DNA. Translation: DAA07760.1.
PIRiS37225.
RefSeqiNP_011025.3. NM_001178990.3.

Genome annotation databases

EnsemblFungiiYER099C; YER099C; YER099C.
GeneIDi856836.
KEGGisce:YER099C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74414 Genomic DNA. Translation: CAA52436.1.
X75075 Genomic DNA. Translation: CAA52969.1.
U18839 Genomic DNA. Translation: AAB64654.1.
AY692977 Genomic DNA. Translation: AAT92996.1.
BK006939 Genomic DNA. Translation: DAA07760.1.
PIRiS37225.
RefSeqiNP_011025.3. NM_001178990.3.

3D structure databases

ProteinModelPortaliP38620.
SMRiP38620. Positions 5-317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36845. 15 interactions.
DIPiDIP-1482N.
IntActiP38620. 4 interactions.
MINTiMINT-404277.

PTM databases

iPTMnetiP38620.

Proteomic databases

MaxQBiP38620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER099C; YER099C; YER099C.
GeneIDi856836.
KEGGisce:YER099C.

Organism-specific databases

EuPathDBiFungiDB:YER099C.
SGDiS000000901. PRS2.

Phylogenomic databases

GeneTreeiENSGT00550000074583.
HOGENOMiHOG000210451.
InParanoidiP38620.
KOiK00948.
OMAiMENGANS.
OrthoDBiEOG78M0B7.

Enzyme and pathway databases

UniPathwayiUPA00087; UER00172.
BioCyciYEAST:YER099C-MONOMER.
ReactomeiR-SCE-73843. 5-Phosphoribose 1-diphosphate biosynthesis.

Miscellaneous databases

NextBioi983141.
PROiP38620.

Family and domain databases

Gene3Di3.40.50.2020. 2 hits.
InterProiIPR000842. PRib_PP_synth_CS.
IPR029099. Pribosyltran_N.
IPR029057. PRTase-like.
IPR005946. Rib-P_diPkinase.
[Graphical view]
PfamiPF14572. Pribosyl_synth. 1 hit.
PF13793. Pribosyltran_N. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01251. ribP_PPkin. 1 hit.
PROSITEiPS00114. PRPP_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phosphoribosylpyrophosphate synthetase (PRS): a new gene family in Saccharomyces cerevisiae."
    Carter A.T., Narbad A., Pearson B.M., Beck K.-F., Logghe M., Contreras R., Schweizer M.
    Yeast 10:1031-1044(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 44827 / SKQ2N.
  2. "Heterooligomeric phosphoribosyl diphosphate synthase of Saccharomyces cerevisiae: combinatorial expression of the five PRS genes in Escherichia coli."
    Hove-Jensen B.
    J. Biol. Chem. 279:40345-40350(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME ACTIVITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae."
    Hernando Y., Carter A.T., Parr A., Hove-Jensen B., Schweizer M.
    J. Biol. Chem. 274:12480-12487(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME ACTIVITY.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKPR2_YEAST
AccessioniPrimary (citable) accession number: P38620
Secondary accession number(s): D3DM06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 11, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8120 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.