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Protein

Ribose-phosphate pyrophosphokinase 2

Gene

PRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).2 Publications

Miscellaneous

Present with 8120 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.2 Publications

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase 2 (PRS2), Ribose-phosphate pyrophosphokinase 4 (PRS4), Ribose-phosphate pyrophosphokinase 1 (PRS1), Ribose-phosphate pyrophosphokinase 5 (PRS5), Ribose-phosphate pyrophosphokinase 3 (PRS3)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi132MagnesiumSequence analysis1
Metal bindingi134MagnesiumSequence analysis1
Metal bindingi143MagnesiumSequence analysis1
Metal bindingi147MagnesiumSequence analysis1

GO - Molecular functioni

GO - Biological processi

  • 5-phosphoribose 1-diphosphate biosynthetic process Source: SGD
  • fungal-type cell wall organization Source: SGD
  • nucleoside metabolic process Source: InterPro
  • nucleotide biosynthetic process Source: UniProtKB-KW
  • ribonucleoside monophosphate biosynthetic process Source: InterPro

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YER099C-MONOMER
ReactomeiR-SCE-73843 5-Phosphoribose 1-diphosphate biosynthesis
UniPathwayiUPA00087; UER00172

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinase 2 (EC:2.7.6.1)
Alternative name(s):
Phosphoribosyl pyrophosphate synthase 2
Gene namesi
Name:PRS2
Synonyms:PRPS2, PRS
Ordered Locus Names:YER099C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER099C
SGDiS000000901 PRS2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410871 – 318Ribose-phosphate pyrophosphokinase 2Add BLAST318

Proteomic databases

MaxQBiP38620
PaxDbiP38620
PRIDEiP38620

PTM databases

iPTMnetiP38620

Interactioni

Protein-protein interaction databases

BioGridi36845, 77 interactors
DIPiDIP-1482N
IntActiP38620, 7 interactors
MINTiP38620
STRINGi4932.YER099C

Structurei

3D structure databases

ProteinModelPortaliP38620
SMRiP38620
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074583
HOGENOMiHOG000210451
InParanoidiP38620
KOiK00948
OMAiFGWARQD
OrthoDBiEOG092C2PL8

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
InterProiView protein in InterPro
IPR000842 PRib_PP_synth_CS
IPR029099 Pribosyltran_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005946 Rib-P_diPkinase
PfamiView protein in Pfam
PF14572 Pribosyl_synth, 1 hit
PF13793 Pribosyltran_N, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01251 ribP_PPkin, 1 hit
PROSITEiView protein in PROSITE
PS00114 PRPP_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P38620-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNSIKLLA GNSHPGLAEL ISQRLGVPLS KVGVYQYSNK ETSVTIGESI
60 70 80 90 100
RDEDVYIIQT GYGEHEINDF LMELLILIHA CKTASVRRIT AVIPNFPYAR
110 120 130 140 150
QDKKDKSRAP ITAKLIANLL ETAGCDHVIT MDLHASQIQG FFHIPVDNLY
160 170 180 190 200
GEPSVLNYIR TKTDFNNAIL VSPDAGGAKR VASLADKLDM NFALIHKERQ
210 220 230 240 250
KANEVSRMLL VGDVAGKSCL LIDDMADTCG TLVKACDTLM DHGAKEVIAI
260 270 280 290 300
VTHGIFSGSA REKLINSRLS RIVCTNTVPV DLDLDIVDQV DISPTIAEAI
310
RRLHNGESVS YLFTHAPV
Length:318
Mass (Da):34,765
Last modified:October 1, 1994 - v1
Checksum:i8B970E98084F5D71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74414 Genomic DNA Translation: CAA52436.1
X75075 Genomic DNA Translation: CAA52969.1
U18839 Genomic DNA Translation: AAB64654.1
AY692977 Genomic DNA Translation: AAT92996.1
BK006939 Genomic DNA Translation: DAA07760.1
PIRiS37225
RefSeqiNP_011025.3, NM_001178990.3

Genome annotation databases

EnsemblFungiiYER099C; YER099C; YER099C
GeneIDi856836
KEGGisce:YER099C

Similar proteinsi

Entry informationi

Entry nameiKPR2_YEAST
AccessioniPrimary (citable) accession number: P38620
Secondary accession number(s): D3DM06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health