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Protein

Cycloartenol synthase

Gene

CAS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts oxidosqualene to cycloartenol and 1% parkeol. Involved in plastid biogenesis. Essential for the male gametophyte function.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = cycloartenol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei257Proton acceptorSequence analysis1
Active sitei483Proton donorSequence analysis1

GO - Molecular functioni

  • cycloartenol synthase activity Source: TAIR

GO - Biological processi

  • pentacyclic triterpenoid biosynthetic process Source: TAIR
  • pollen development Source: TAIR
  • thylakoid membrane organization Source: TAIR

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BioCyciARA:AT2G07050-MONOMER
MetaCyc:AT2G07050-MONOMER
BRENDAi5.4.99.8 399
ReactomeiR-ATH-191273 Cholesterol biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloartenol synthase (EC:5.4.99.8)
Short name:
AtCYC
Alternative name(s):
2,3-epoxysqualene--cycloartenol cyclase
Gene namesi
Name:CAS1
Synonyms:CYC
Ordered Locus Names:At2g07050
ORF Names:T4E14.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G07050
TAIRilocus:2060121 AT2G07050

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Albino phenotype leading to lethality.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi410Y → C: Produces lanosterol instead of cycloartenol. 3 Publications1
Mutagenesisi410Y → T: Produces 65% lanosterol, 2% parkeol and 33% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol, 0.6% parkeol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with V-481. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with N-477 or Q-477 and V-481. 3 Publications1
Mutagenesisi469A → V: Produces lanosterol and achilleol A instead of cycloartenol. 1 Publication1
Mutagenesisi477H → N: Produces 88% lanosterol and 12% parkeol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481. 3 Publications1
Mutagenesisi477H → Q: Produces 22% lanosterol, 73% parkeol and 5% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481. 3 Publications1
Mutagenesisi477H → Y: Produces lanosterol instead of cycloartenol. 3 Publications1
Mutagenesisi481I → T: Produces lanosterol and achilleol A instead of cycloartenol. 3 Publications1
Mutagenesisi481I → V: Produces 24% lanosterol, 20% parkeol and 56% cycloartenol. Produces 75% lanosterol, 0.6% parkeol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410. 3 Publications1
Mutagenesisi532Y → H: Produces lanosterol and achilleol A instead of cycloartenol. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5356

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000726621 – 759Cycloartenol synthaseAdd BLAST759

Proteomic databases

PaxDbiP38605
PRIDEiP38605

PTM databases

iPTMnetiP38605

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, inflorescences and siliques.1 Publication

Gene expression databases

ExpressionAtlasiP38605 baseline and differential
GenevisibleiP38605 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G07050.1

Chemistry databases

BindingDBiP38605

Structurei

3D structure databases

ProteinModelPortaliP38605
SMRiP38605
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati147 – 188PFTB 1Add BLAST42
Repeati512 – 557PFTB 2Add BLAST46
Repeati589 – 629PFTB 3Add BLAST41
Repeati638 – 679PFTB 4Add BLAST42
Repeati700 – 741PFTB 5Add BLAST42

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0497 Eukaryota
COG1657 LUCA
HOGENOMiHOG000234317
InParanoidiP38605
KOiK01853
OMAiGRQTWTY
OrthoDBiEOG0936031Q
PhylomeDBiP38605

Family and domain databases

CDDicd02892 SQCY_1, 1 hit
InterProiView protein in InterPro
IPR032696 SQ_cyclase_C
IPR032697 SQ_cyclase_N
IPR018333 Squalene_cyclase
IPR002365 Terpene_synthase_CS
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF13243 SQHop_cyclase_C, 1 hit
PF13249 SQHop_cyclase_N, 1 hit
SUPFAMiSSF48239 SSF48239, 2 hits
TIGRFAMsiTIGR01787 squalene_cyclas, 1 hit
PROSITEiView protein in PROSITE
PS01074 TERPENE_SYNTHASES, 1 hit

Sequencei

Sequence statusi: Complete.

P38605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKLKIAEGG SPWLRTTNNH VGRQFWEFDP NLGTPEDLAA VEEARKSFSD
60 70 80 90 100
NRFVQKHSAD LLMRLQFSRE NLISPVLPQV KIEDTDDVTE EMVETTLKRG
110 120 130 140 150
LDFYSTIQAH DGHWPGDYGG PMFLLPGLII TLSITGALNT VLSEQHKQEM
160 170 180 190 200
RRYLYNHQNE DGGWGLHIEG PSTMFGSVLN YVTLRLLGEG PNDGDGDMEK
210 220 230 240 250
GRDWILNHGG ATNITSWGKM WLSVLGAFEW SGNNPLPPEI WLLPYFLPIH
260 270 280 290 300
PGRMWCHCRM VYLPMSYLYG KRFVGPITST VLSLRKELFT VPYHEVNWNE
310 320 330 340 350
ARNLCAKEDL YYPHPLVQDI LWASLHKIVE PVLMRWPGAN LREKAIRTAI
360 370 380 390 400
EHIHYEDENT RYICIGPVNK VLNMLCCWVE DPNSEAFKLH LPRIHDFLWL
410 420 430 440 450
AEDGMKMQGY NGSQLWDTGF AIQAILATNL VEEYGPVLEK AHSFVKNSQV
460 470 480 490 500
LEDCPGDLNY WYRHISKGAW PFSTADHGWP ISDCTAEGLK AALLLSKVPK
510 520 530 540 550
AIVGEPIDAK RLYEAVNVII SLQNADGGLA TYELTRSYPW LELINPAETF
560 570 580 590 600
GDIVIDYPYV ECTSAAIQAL ISFRKLYPGH RKKEVDECIE KAVKFIESIQ
610 620 630 640 650
AADGSWYGSW AVCFTYGTWF GVKGLVAVGK TLKNSPHVAK ACEFLLSKQQ
660 670 680 690 700
PSGGWGESYL SCQDKVYSNL DGNRSHVVNT AWAMLALIGA GQAEVDRKPL
710 720 730 740 750
HRAARYLINA QMENGDFPQQ EIMGVFNRNC MITYAAYRNI FPIWALGEYR

CQVLLQQGE
Length:759
Mass (Da):86,033
Last modified:June 20, 2002 - v2
Checksum:iDBA75CC57BB1F74D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti501A → E in AAC04931 (PubMed:7505443).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02555 mRNA Translation: AAC04931.1
AC005171 Genomic DNA Translation: AAM15015.1
CP002685 Genomic DNA Translation: AEC06032.1
AY094394 mRNA Translation: AAM19773.1
BT001118 mRNA Translation: AAN64509.1
PIRiA49398
H84481
RefSeqiNP_178722.1, NM_126681.3
UniGeneiAt.10550

Genome annotation databases

EnsemblPlantsiAT2G07050.1; AT2G07050.1; AT2G07050
GeneIDi815275
GrameneiAT2G07050.1; AT2G07050.1; AT2G07050
KEGGiath:AT2G07050

Similar proteinsi

Entry informationi

Entry nameiCAS1_ARATH
AccessioniPrimary (citable) accession number: P38605
Secondary accession number(s): P92967
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: June 20, 2002
Last modified: April 25, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health