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Protein

Cycloartenol synthase

Gene

CAS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts oxidosqualene to cycloartenol and 1% parkeol. Involved in plastid biogenesis. Essential for the male gametophyte function.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = cycloartenol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei257 – 2571Proton acceptorSequence analysis
Active sitei483 – 4831Proton donorSequence analysis

GO - Molecular functioni

  • cycloartenol synthase activity Source: TAIR

GO - Biological processi

  • pentacyclic triterpenoid biosynthetic process Source: TAIR
  • pollen development Source: TAIR
  • thylakoid membrane organization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciARA:AT2G07050-MONOMER.
MetaCyc:AT2G07050-MONOMER.
BRENDAi5.4.99.8. 399.
ReactomeiR-ATH-191273. Cholesterol biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Cycloartenol synthase (EC:5.4.99.8)
Short name:
AtCYC
Alternative name(s):
2,3-epoxysqualene--cycloartenol cyclase
Gene namesi
Name:CAS1
Synonyms:CYC
Ordered Locus Names:At2g07050
ORF Names:T4E14.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G07050.

Subcellular locationi

GO - Cellular componenti

  • vacuole Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Albino phenotype leading to lethality.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi410 – 4101Y → C: Produces lanosterol instead of cycloartenol. 3 Publications
Mutagenesisi410 – 4101Y → T: Produces 65% lanosterol, 2% parkeol and 33% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol, 0.6% parkeol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with V-481. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with N-477 or Q-477 and V-481. 3 Publications
Mutagenesisi469 – 4691A → V: Produces lanosterol and achilleol A instead of cycloartenol. 1 Publication
Mutagenesisi477 – 4771H → N: Produces 88% lanosterol and 12% parkeol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481. 3 Publications
Mutagenesisi477 – 4771H → Q: Produces 22% lanosterol, 73% parkeol and 5% 9beta-delta7-lanosterol instead of cycloartenol. Produces 75% lanosterol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410 and V-481. 3 Publications
Mutagenesisi477 – 4771H → Y: Produces lanosterol instead of cycloartenol. 3 Publications
Mutagenesisi481 – 4811I → T: Produces lanosterol and achilleol A instead of cycloartenol. 3 Publications
Mutagenesisi481 – 4811I → V: Produces 24% lanosterol, 20% parkeol and 56% cycloartenol. Produces 75% lanosterol, 0.6% parkeol and 24% 9beta-delta7-lanosterol instead of cycloartenol; when associated with T-410. 3 Publications
Mutagenesisi532 – 5321Y → H: Produces lanosterol and achilleol A instead of cycloartenol. 1 Publication

Chemistry

ChEMBLiCHEMBL5356.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759Cycloartenol synthasePRO_0000072662Add
BLAST

Proteomic databases

PaxDbiP38605.
PRIDEiP38605.

PTM databases

iPTMnetiP38605.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, inflorescences and siliques.1 Publication

Gene expression databases

GenevisibleiP38605. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G07050.1.

Chemistry

BindingDBiP38605.

Structurei

3D structure databases

ProteinModelPortaliP38605.
SMRiP38605. Positions 100-749.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati147 – 18842PFTB 1Add
BLAST
Repeati512 – 55746PFTB 2Add
BLAST
Repeati589 – 62941PFTB 3Add
BLAST
Repeati638 – 67942PFTB 4Add
BLAST
Repeati700 – 74142PFTB 5Add
BLAST

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated
Contains 5 PFTB repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiP38605.
KOiK01853.
OMAiSQWYRHI.
OrthoDBiEOG0936031Q.
PhylomeDBiP38605.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKLKIAEGG SPWLRTTNNH VGRQFWEFDP NLGTPEDLAA VEEARKSFSD
60 70 80 90 100
NRFVQKHSAD LLMRLQFSRE NLISPVLPQV KIEDTDDVTE EMVETTLKRG
110 120 130 140 150
LDFYSTIQAH DGHWPGDYGG PMFLLPGLII TLSITGALNT VLSEQHKQEM
160 170 180 190 200
RRYLYNHQNE DGGWGLHIEG PSTMFGSVLN YVTLRLLGEG PNDGDGDMEK
210 220 230 240 250
GRDWILNHGG ATNITSWGKM WLSVLGAFEW SGNNPLPPEI WLLPYFLPIH
260 270 280 290 300
PGRMWCHCRM VYLPMSYLYG KRFVGPITST VLSLRKELFT VPYHEVNWNE
310 320 330 340 350
ARNLCAKEDL YYPHPLVQDI LWASLHKIVE PVLMRWPGAN LREKAIRTAI
360 370 380 390 400
EHIHYEDENT RYICIGPVNK VLNMLCCWVE DPNSEAFKLH LPRIHDFLWL
410 420 430 440 450
AEDGMKMQGY NGSQLWDTGF AIQAILATNL VEEYGPVLEK AHSFVKNSQV
460 470 480 490 500
LEDCPGDLNY WYRHISKGAW PFSTADHGWP ISDCTAEGLK AALLLSKVPK
510 520 530 540 550
AIVGEPIDAK RLYEAVNVII SLQNADGGLA TYELTRSYPW LELINPAETF
560 570 580 590 600
GDIVIDYPYV ECTSAAIQAL ISFRKLYPGH RKKEVDECIE KAVKFIESIQ
610 620 630 640 650
AADGSWYGSW AVCFTYGTWF GVKGLVAVGK TLKNSPHVAK ACEFLLSKQQ
660 670 680 690 700
PSGGWGESYL SCQDKVYSNL DGNRSHVVNT AWAMLALIGA GQAEVDRKPL
710 720 730 740 750
HRAARYLINA QMENGDFPQQ EIMGVFNRNC MITYAAYRNI FPIWALGEYR

CQVLLQQGE
Length:759
Mass (Da):86,033
Last modified:June 20, 2002 - v2
Checksum:iDBA75CC57BB1F74D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti501 – 5011A → E in AAC04931 (PubMed:7505443).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02555 mRNA. Translation: AAC04931.1.
AC005171 Genomic DNA. Translation: AAM15015.1.
CP002685 Genomic DNA. Translation: AEC06032.1.
AY094394 mRNA. Translation: AAM19773.1.
BT001118 mRNA. Translation: AAN64509.1.
PIRiA49398.
H84481.
RefSeqiNP_178722.1. NM_126681.2.
UniGeneiAt.10550.

Genome annotation databases

EnsemblPlantsiAT2G07050.1; AT2G07050.1; AT2G07050.
GeneIDi815275.
GrameneiAT2G07050.1; AT2G07050.1; AT2G07050.
KEGGiath:AT2G07050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02555 mRNA. Translation: AAC04931.1.
AC005171 Genomic DNA. Translation: AAM15015.1.
CP002685 Genomic DNA. Translation: AEC06032.1.
AY094394 mRNA. Translation: AAM19773.1.
BT001118 mRNA. Translation: AAN64509.1.
PIRiA49398.
H84481.
RefSeqiNP_178722.1. NM_126681.2.
UniGeneiAt.10550.

3D structure databases

ProteinModelPortaliP38605.
SMRiP38605. Positions 100-749.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G07050.1.

Chemistry

BindingDBiP38605.
ChEMBLiCHEMBL5356.

PTM databases

iPTMnetiP38605.

Proteomic databases

PaxDbiP38605.
PRIDEiP38605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G07050.1; AT2G07050.1; AT2G07050.
GeneIDi815275.
GrameneiAT2G07050.1; AT2G07050.1; AT2G07050.
KEGGiath:AT2G07050.

Organism-specific databases

TAIRiAT2G07050.

Phylogenomic databases

eggNOGiKOG0497. Eukaryota.
COG1657. LUCA.
HOGENOMiHOG000234317.
InParanoidiP38605.
KOiK01853.
OMAiSQWYRHI.
OrthoDBiEOG0936031Q.
PhylomeDBiP38605.

Enzyme and pathway databases

BioCyciARA:AT2G07050-MONOMER.
MetaCyc:AT2G07050-MONOMER.
BRENDAi5.4.99.8. 399.
ReactomeiR-ATH-191273. Cholesterol biosynthesis.

Miscellaneous databases

PROiP38605.

Gene expression databases

GenevisibleiP38605. AT.

Family and domain databases

Gene3Di1.50.10.20. 2 hits.
InterProiIPR032696. SQ_cyclase_C.
IPR032697. SQ_cyclase_N.
IPR018333. Squalene_cyclase.
IPR002365. Terpene_synthase_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF13243. SQHop_cyclase_C. 1 hit.
PF13249. SQHop_cyclase_N. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
TIGRFAMsiTIGR01787. squalene_cyclas. 1 hit.
PROSITEiPS01074. TERPENE_SYNTHASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAS1_ARATH
AccessioniPrimary (citable) accession number: P38605
Secondary accession number(s): P92967
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: June 20, 2002
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.