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Protein

Lanosterol synthase

Gene

ERG7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus.

Miscellaneous

Present with 2193 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

(3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol.

Enzyme regulationi

ERG27 is required for activity.1 Publication

pH dependencei

Optimum pH is 7.1 Publication

Temperature dependencei

Optimum temperature is 35 degrees Celsius.1 Publication

Pathwayi: lanosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes lanosterol from farnesyl diphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Squalene synthase (ERG9)
  2. Squalene monooxygenase (ERG1)
  3. Lanosterol synthase (ERG7)
This subpathway is part of the pathway lanosterol biosynthesis, which is itself part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lanosterol from farnesyl diphosphate, the pathway lanosterol biosynthesis and in Terpene metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei234Proton acceptorBy similarity1
Active sitei456Proton donorBy similarity1

GO - Molecular functioni

  • lanosterol synthase activity Source: SGD

GO - Biological processi

  • ergosterol biosynthetic process Source: SGD

Keywordsi

Molecular functionIsomerase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YHR072W-MONOMER
YEAST:YHR072W-MONOMER
BRENDAi5.4.99.7 984
ReactomeiR-SCE-191273 Cholesterol biosynthesis
SABIO-RKP38604
UniPathwayiUPA00767; UER00753

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol synthase (EC:5.4.99.7)
Alternative name(s):
2,3-epoxysqualene--lanosterol cyclase
Oxidosqualene--lanosterol cyclase
Short name:
OSC
Gene namesi
Name:ERG7
Ordered Locus Names:YHR072W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR072W
SGDiS000001114 ERG7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi457C → D: Reduces thermal stability and enzyme activity; when associated with A-526 or C-526. No effect; when associated with D-526 or Q-526. 1 Publication1
Mutagenesisi526E → A or C: Reduces thermal stability and enzyme activity; when associated with D-457. 1 Publication1
Mutagenesisi526E → D or Q: No effect; when associated with D-457. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5355

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000726572 – 731Lanosterol synthaseAdd BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP38604
PaxDbiP38604
PRIDEiP38604

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ERG27Q124524EBI-6572,EBI-38132

Protein-protein interaction databases

BioGridi36505, 285 interactors
DIPiDIP-8319N
IntActiP38604, 19 interactors
MINTiP38604
STRINGi4932.YHR072W

Chemistry databases

BindingDBiP38604

Structurei

3D structure databases

ProteinModelPortaliP38604
SMRiP38604
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati125 – 167PFTB 1Add BLAST43
Repeati566 – 603PFTB 2Add BLAST38
Repeati615 – 661PFTB 3Add BLAST47

Sequence similaritiesi

Belongs to the terpene cyclase/mutase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000011570
HOGENOMiHOG000234317
InParanoidiP38604
KOiK01852
OMAiGRQTWTY
OrthoDBiEOG092C0KMX

Family and domain databases

CDDicd02892 SQCY_1, 1 hit
InterProiView protein in InterPro
IPR032696 SQ_cyclase_C
IPR032697 SQ_cyclase_N
IPR018333 Squalene_cyclase
IPR002365 Terpene_synthase_CS
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF13243 SQHop_cyclase_C, 1 hit
PF13249 SQHop_cyclase_N, 1 hit
SUPFAMiSSF48239 SSF48239, 2 hits
TIGRFAMsiTIGR01787 squalene_cyclas, 1 hit
PROSITEiView protein in PROSITE
PS01074 TERPENE_SYNTHASES, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEFYSDTIG LPKTDPRLWR LRTDELGRES WEYLTPQQAA NDPPSTFTQW
60 70 80 90 100
LLQDPKFPQP HPERNKHSPD FSAFDACHNG ASFFKLLQEP DSGIFPCQYK
110 120 130 140 150
GPMFMTIGYV AVNYIAGIEI PEHERIELIR YIVNTAHPVD GGWGLHSVDK
160 170 180 190 200
STVFGTVLNY VILRLLGLPK DHPVCAKARS TLLRLGGAIG SPHWGKIWLS
210 220 230 240 250
ALNLYKWEGV NPAPPETWLL PYSLPMHPGR WWVHTRGVYI PVSYLSLVKF
260 270 280 290 300
SCPMTPLLEE LRNEIYTKPF DKINFSKNRN TVCGVDLYYP HSTTLNIANS
310 320 330 340 350
LVVFYEKYLR NRFIYSLSKK KVYDLIKTEL QNTDSLCIAP VNQAFCALVT
360 370 380 390 400
LIEEGVDSEA FQRLQYRFKD ALFHGPQGMT IMGTNGVQTW DCAFAIQYFF
410 420 430 440 450
VAGLAERPEF YNTIVSAYKF LCHAQFDTEC VPGSYRDKRK GAWGFSTKTQ
460 470 480 490 500
GYTVADCTAE AIKAIIMVKN SPVFSEVHHM ISSERLFEGI DVLLNLQNIG
510 520 530 540 550
SFEYGSFATY EKIKAPLAME TLNPAEVFGN IMVEYPYVEC TDSSVLGLTY
560 570 580 590 600
FHKYFDYRKE EIRTRIRIAI EFIKKSQLPD GSWYGSWGIC FTYAGMFALE
610 620 630 640 650
ALHTVGETYE NSSTVRKGCD FLVSKQMKDG GWGESMKSSE LHSYVDSEKS
660 670 680 690 700
LVVQTAWALI ALLFAEYPNK EVIDRGIDLL KNRQEESGEW KFESVEGVFN
710 720 730
HSCAIEYPSY RFLFPIKALG MYSRAYETHT L
Length:731
Mass (Da):83,460
Last modified:July 27, 2011 - v6
Checksum:i9CE85BE1A7F5F52A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61H → N in AAA16975 (PubMed:8134375).Curated1
Sequence conflicti530N → D in AAA16975 (PubMed:8134375).Curated1
Sequence conflicti530N → D in AAB68891 (PubMed:8091229).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti359 – 731Missing in strain: SGL9; loss of activity. 1 PublicationAdd BLAST373

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04841 Unassigned DNA Translation: AAA16975.1
U23488 Genomic DNA Translation: AAA64377.1
U60996 Genomic DNA Translation: AAB08472.1
U10556 Genomic DNA Translation: AAB68891.1
AY693043 Genomic DNA Translation: AAT93062.1
BK006934 Genomic DNA Translation: DAA06765.2
PIRiS46813
RefSeqiNP_011939.2, NM_001179202.2

Genome annotation databases

EnsemblFungiiYHR072W; YHR072W; YHR072W
GeneIDi856470
KEGGisce:YHR072W

Similar proteinsi

Entry informationi

Entry nameiERG7_YEAST
AccessioniPrimary (citable) accession number: P38604
Secondary accession number(s): D3DL21, Q92327
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 163 of the entry and version 6 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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