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P38590

- MSG5_YEAST

UniProt

P38590 - MSG5_YEAST

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Protein
Tyrosine-protein phosphatase MSG5
Gene
MSG5, YNL053W, N2480, YNL2480W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Dual specificity phosphatase that dephosphorylates MAP kinase FUS3 on both a Tyr and a Ser or Thr. Has a role in adaptation to pheromone.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei319 – 3191Phosphocysteine intermediate

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC
  2. protein tyrosine/serine/threonine phosphatase activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
  2. cell cycle Source: UniProtKB-KW
  3. inactivation of MAPK activity involved in cell wall organization or biogenesis Source: SGD
  4. inactivation of MAPK activity involved in conjugation with cellular fusion Source: SGD
  5. protein dephosphorylation Source: SGD
  6. regulation of MAPK export from nucleus Source: SGD
  7. regulation of fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

BioCyciYEAST:G3O-33085-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase MSG5 (EC:3.1.3.48)
Gene namesi
Name:MSG5
Ordered Locus Names:YNL053W
ORF Names:N2480, YNL2480W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL053w.
SGDiS000004998. MSG5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi319 – 3191C → A: Loss of activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Tyrosine-protein phosphatase MSG5
PRO_0000094916Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221Phosphoserine1 Publication
Modified residuei98 – 981Phosphoserine1 Publication
Modified residuei151 – 1511Phosphoserine1 Publication
Modified residuei178 – 1781Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP38590.

Expressioni

Inductioni

By pheromone.

Gene expression databases

GenevestigatoriP38590.

Interactioni

Protein-protein interaction databases

BioGridi35771. 65 interactions.
DIPiDIP-5538N.
IntActiP38590. 9 interactions.
MINTiMINT-543826.
STRINGi4932.YNL053W.

Structurei

3D structure databases

ProteinModelPortaliP38590.
SMRiP38590. Positions 229-374.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00680000100889.
HOGENOMiHOG000066070.
KOiK11240.
OMAiVDNESIS.
OrthoDBiEOG7HF1WG.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38590-1 [UniParc]FASTAAdd to Basket

« Hide

MQFHSDKQHL DSKTDIDFKP NSPRSLQNRN TKNLSLDIAA LHPLMEFSSP    50
SQDVPGSVKF PSPTPLNLFM KPKPIVLEKC PPKVSPRPTP PSLSMRRSEA 100
SIYTLPTSLK NRTVSPSVYT KSSTVSSISK LSSSSPLSSF SEKPHLNRVH 150
SLSVKTKDLK LKGIRGRSQT ISGLETSTPI SSTREGTLDS TDVNRFSNQK 200
NMQTTLIFPE EDSDLNIDMV HAEIYQRTVY LDGPLLVLPP NLYLYSEPKL 250
EDILSFDLVI NVAKEIPNLE FLIPPEMAHK IKYYHIEWTH TSKIVKDLSR 300
LTRIIHTAHS QGKKILVHCQ CGVSRSASLI VAYIMRYYGL SLNDAYNKLK 350
GVAKDISPNM GLIFQLMEWG TMLSKNSPGE EGETVHMPEE DDIGNNEVSS 400
TTKSYSSASF RSFPMVTNLS SSPNDSSVNS SEVTPRTPAT LTGARTALAT 450
ERGEDDEHCK SLSQPADSLE ASVDNESIST APEQMMFLP 489
Length:489
Mass (Da):54,217
Last modified:October 1, 1996 - v2
Checksum:i0D02380B62EA48DC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti347 – 3482NK → DE in BAA04485. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17548 Genomic DNA. Translation: BAA04485.1.
U12141 Genomic DNA. Translation: AAA99659.1.
Z71329 Genomic DNA. Translation: CAA95922.1.
X02561 Genomic DNA. Translation: CAA26402.1.
BK006947 Genomic DNA. Translation: DAA10492.1.
PIRiS58725.
RefSeqiNP_014345.3. NM_001182892.3.

Genome annotation databases

EnsemblFungiiYNL053W; YNL053W; YNL053W.
GeneIDi855674.
KEGGisce:YNL053W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17548 Genomic DNA. Translation: BAA04485.1 .
U12141 Genomic DNA. Translation: AAA99659.1 .
Z71329 Genomic DNA. Translation: CAA95922.1 .
X02561 Genomic DNA. Translation: CAA26402.1 .
BK006947 Genomic DNA. Translation: DAA10492.1 .
PIRi S58725.
RefSeqi NP_014345.3. NM_001182892.3.

3D structure databases

ProteinModelPortali P38590.
SMRi P38590. Positions 229-374.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35771. 65 interactions.
DIPi DIP-5538N.
IntActi P38590. 9 interactions.
MINTi MINT-543826.
STRINGi 4932.YNL053W.

Proteomic databases

PaxDbi P38590.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL053W ; YNL053W ; YNL053W .
GeneIDi 855674.
KEGGi sce:YNL053W.

Organism-specific databases

CYGDi YNL053w.
SGDi S000004998. MSG5.

Phylogenomic databases

eggNOGi COG2453.
GeneTreei ENSGT00680000100889.
HOGENOMi HOG000066070.
KOi K11240.
OMAi VDNESIS.
OrthoDBi EOG7HF1WG.

Enzyme and pathway databases

BioCyci YEAST:G3O-33085-MONOMER.

Miscellaneous databases

NextBioi 979960.

Gene expression databases

Genevestigatori P38590.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view ]
PANTHERi PTHR10159. PTHR10159. 1 hit.
Pfami PF00782. DSPc. 1 hit.
[Graphical view ]
SMARTi SM00195. DSPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae."
    Doi K., Gartner A., Ammerer G., Errede B., Shinkawa H., Sugimoto K., Matsumoto K.
    EMBO J. 13:61-70(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Bergez P., Doignon F., Crouzet M.
    Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Primary structure of a gene for subunit V of the cytochrome c oxidase from Saccharomyces cerevisiae."
    Seraphin B., Simon M., Faye G.
    Curr. Genet. 9:435-439(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 415-489.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22; SER-151 AND THR-178, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-98, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMSG5_YEAST
AccessioniPrimary (citable) accession number: P38590
Secondary accession number(s): D6W1C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 538 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi