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Protein

Tyrosine-protein phosphatase MSG5

Gene

MSG5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dual specificity phosphatase that dephosphorylates MAP kinase FUS3 on both a Tyr and a Ser or Thr. Has a role in adaptation to pheromone.

Miscellaneous

Present with 538 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei319Phosphocysteine intermediate1

GO - Molecular functioni

GO - Biological processi

  • adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
  • cell cycle Source: UniProtKB-KW
  • inactivation of MAPK activity Source: SGD
  • inactivation of MAPK activity involved in cell wall organization or biogenesis Source: SGD
  • protein dephosphorylation Source: SGD
  • regulation of fungal-type cell wall organization Source: SGD
  • regulation of MAPK export from nucleus Source: SGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle

Enzyme and pathway databases

BioCyciYEAST:G3O-33085-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase MSG5 (EC:3.1.3.48)
Gene namesi
Name:MSG5
Ordered Locus Names:YNL053W
ORF Names:N2480, YNL2480W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL053W
SGDiS000004998 MSG5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi319C → A: Loss of activity. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000949161 – 489Tyrosine-protein phosphatase MSG5Add BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22PhosphoserineCombined sources1
Modified residuei98PhosphoserineCombined sources1
Modified residuei151PhosphoserineCombined sources1
Modified residuei178PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP38590
PRIDEiP38590

PTM databases

iPTMnetiP38590

Expressioni

Inductioni

By pheromone.

Interactioni

Protein-protein interaction databases

BioGridi35771151 interactors.
DIPiDIP-5538N
ELMiP38590
IntActiP38590 20 interactors.
MINTiP38590
STRINGi4932.YNL053W

Structurei

3D structure databases

ProteinModelPortaliP38590
SMRiP38590
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00680000100889
HOGENOMiHOG000066070
InParanoidiP38590
KOiK11240
OrthoDBiEOG092C1CH8

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.101 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequencei

Sequence statusi: Complete.

P38590-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQFHSDKQHL DSKTDIDFKP NSPRSLQNRN TKNLSLDIAA LHPLMEFSSP
60 70 80 90 100
SQDVPGSVKF PSPTPLNLFM KPKPIVLEKC PPKVSPRPTP PSLSMRRSEA
110 120 130 140 150
SIYTLPTSLK NRTVSPSVYT KSSTVSSISK LSSSSPLSSF SEKPHLNRVH
160 170 180 190 200
SLSVKTKDLK LKGIRGRSQT ISGLETSTPI SSTREGTLDS TDVNRFSNQK
210 220 230 240 250
NMQTTLIFPE EDSDLNIDMV HAEIYQRTVY LDGPLLVLPP NLYLYSEPKL
260 270 280 290 300
EDILSFDLVI NVAKEIPNLE FLIPPEMAHK IKYYHIEWTH TSKIVKDLSR
310 320 330 340 350
LTRIIHTAHS QGKKILVHCQ CGVSRSASLI VAYIMRYYGL SLNDAYNKLK
360 370 380 390 400
GVAKDISPNM GLIFQLMEWG TMLSKNSPGE EGETVHMPEE DDIGNNEVSS
410 420 430 440 450
TTKSYSSASF RSFPMVTNLS SSPNDSSVNS SEVTPRTPAT LTGARTALAT
460 470 480
ERGEDDEHCK SLSQPADSLE ASVDNESIST APEQMMFLP
Length:489
Mass (Da):54,217
Last modified:October 1, 1996 - v2
Checksum:i0D02380B62EA48DC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti347 – 348NK → DE in BAA04485 (PubMed:8306972).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17548 Genomic DNA Translation: BAA04485.1
U12141 Genomic DNA Translation: AAA99659.1
Z71329 Genomic DNA Translation: CAA95922.1
X02561 Genomic DNA Translation: CAA26402.1
BK006947 Genomic DNA Translation: DAA10492.1
PIRiS58725
RefSeqiNP_014345.3, NM_001182892.3

Genome annotation databases

EnsemblFungiiYNL053W; YNL053W; YNL053W
GeneIDi855674
KEGGisce:YNL053W

Similar proteinsi

Entry informationi

Entry nameiMSG5_YEAST
AccessioniPrimary (citable) accession number: P38590
Secondary accession number(s): D6W1C6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome