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Protein

Tubulin--tyrosine ligase

Gene

Ttl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.1 Publication

Catalytic activityi

ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate.By similarity

Cofactori

Protein has several cofactor binding sites:

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • tubulin-tyrosine ligase activity Source: MGI

GO - Biological processi

  • C-terminal protein-tyrosinylation Source: MGI
  • microtubule cytoskeleton organization Source: MGI
  • positive regulation of mitotic cell cycle Source: MGI
  • regulation of axon extension Source: MGI
  • regulation of metaphase plate congression Source: MGI

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin--tyrosine ligase (EC:6.3.2.25By similarity)
Short name:
TTL
Gene namesi
Name:Ttl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1916987 Ttl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002124351 – 377Tubulin--tyrosine ligaseAdd BLAST377

Proteomic databases

EPDiP38585
MaxQBiP38585
PaxDbiP38585
PRIDEiP38585

PTM databases

PhosphoSitePlusiP38585

Expressioni

Gene expression databases

BgeeiENSMUSG00000027394
CleanExiMM_TTL
ExpressionAtlasiP38585 baseline and differential
GenevisibleiP38585 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000046883

Structurei

3D structure databases

ProteinModelPortaliP38585
SMRiP38585
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 370TTLPROSITE-ProRule annotationAdd BLAST368

Sequence similaritiesi

Belongs to the tubulin--tyrosine ligase family.Curated

Phylogenomic databases

eggNOGiKOG2157 Eukaryota
ENOG410XQDM LUCA
GeneTreeiENSGT00900000141131
HOGENOMiHOG000231734
HOVERGENiHBG016579
InParanoidiP38585
KOiK06047
OMAiCLMSVEP
OrthoDBiEOG091G08SJ
PhylomeDBiP38585
TreeFamiTF350555

Family and domain databases

InterProiView protein in InterPro
IPR004344 TTL/TTLL_fam
PfamiView protein in Pfam
PF03133 TTL, 1 hit
PROSITEiView protein in PROSITE
PS51221 TTL, 1 hit

Sequencei

Sequence statusi: Complete.

P38585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTFVVRDEN SSVYAEVSRL LLATGYWKRL RRDNPRFNLM LGERNRLPFG
60 70 80 90 100
RLGHEPGLAQ LVNYYRGADK LCRKASLVKL VKTSPELSES CSWFPESYVI
110 120 130 140 150
YPTNLKTPVA PAQNGIQLPV SNSRTDEREF FLASYNRKKE DGEGNVWIAK
160 170 180 190 200
SSAGAKGEGI LISSEASELL DFIDSQGQVH VIQKYLERPL LLEPGHRKFD
210 220 230 240 250
IRSWVLVDHQ YNIYLYREGV LRTASEPYHV DNFQDKTCHL TNHCIQKEYS
260 270 280 290 300
KNYGKYEEGN EMFFEEFNQY LTSALNITLE SSILLQIKHI IRSCLMSVEP
310 320 330 340 350
AISTKHLPYQ SFQLLGFDFM VDEELKVWLI EVNGAPACAQ KLYAELCQGI
360 370
VDIAISSVFP PPDTEQVPQQ PAAFVKL
Length:377
Mass (Da):43,136
Last modified:May 10, 2004 - v2
Checksum:i4D4E75243BB2CEDD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti222R → Q (PubMed:8093886).Curated1
Sequence conflicti230V → M (PubMed:8093886).Curated1
Sequence conflicti239H → Q (PubMed:8093886).Curated1
Sequence conflicti251K → N in AAH24414 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018513 mRNA Translation: AAH18513.1
BC024414 mRNA Translation: AAH24414.1
CCDSiCCDS16719.1
RefSeqiNP_081468.1, NM_027192.2
UniGeneiMm.33909
Mm.479291

Genome annotation databases

EnsembliENSMUST00000035812; ENSMUSP00000046883; ENSMUSG00000027394
GeneIDi69737
KEGGimmu:69737
UCSCiuc008mhe.2 mouse

Similar proteinsi

Entry informationi

Entry nameiTTL_MOUSE
AccessioniPrimary (citable) accession number: P38585
Secondary accession number(s): Q8R1L7, Q8VEG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 10, 2004
Last modified: March 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health