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Protein

Inorganic pyrophosphatase

Gene

ppa

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Diphosphate + H2O = 2 phosphate.

Cofactori

Mg2+Note: Binds 4 Mg2+ ions per subunit. Other metal ions can support activity, but at a lower rate. Two Mg2+ ions are required for the activation of the enzyme and are present before substrate binds, two additional Mg2+ ions form complexes with substrate and product.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Magnesium 1
Metal bindingi71 – 711Magnesium 1
Metal bindingi71 – 711Magnesium 2
Metal bindingi103 – 1031Magnesium 1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-2015-MONOMER.
BRENDAi3.6.1.1. 2305.

Names & Taxonomyi

Protein namesi
Recommended name:
Inorganic pyrophosphatase (EC:3.6.1.1)
Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name:
PPase
Gene namesi
Name:ppa
Ordered Locus Names:TTHA1965
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 175174Inorganic pyrophosphatasePRO_0000137535Add
BLAST

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi300852.TTHA1965.

Structurei

Secondary structure

1
175
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Turni11 – 155Combined sources
Beta strandi16 – 238Combined sources
Beta strandi29 – 346Combined sources
Turni35 – 384Combined sources
Beta strandi39 – 457Combined sources
Beta strandi47 – 493Combined sources
Beta strandi53 – 586Combined sources
Beta strandi71 – 755Combined sources
Beta strandi85 – 10016Combined sources
Beta strandi102 – 1109Combined sources
Helixi114 – 1163Combined sources
Helixi122 – 1243Combined sources
Helixi127 – 13913Combined sources
Helixi142 – 1476Combined sources
Beta strandi151 – 1577Combined sources
Helixi159 – 17416Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PRDX-ray2.00A2-175[»]
ProteinModelPortaliP38576.
SMRiP38576. Positions 2-175.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38576.

Family & Domainsi

Sequence similaritiesi

Belongs to the PPase family.Curated

Phylogenomic databases

eggNOGiENOG4105F0N. Bacteria.
COG0221. LUCA.
HOGENOMiHOG000236472.
KOiK01507.
OMAiDGVANYK.
PhylomeDBiP38576.

Family and domain databases

CDDicd00412. pyrophosphatase. 1 hit.
Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38576-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLKSLPVG DKAPEVVHMV IEVPRGSGNK YEYDPDLGAI KLDRVLPGAQ
60 70 80 90 100
FYPGDYGFIP STLAEDGDPL DGLVLSTYPL LPGVVVEVRV VGLLLMEDEK
110 120 130 140 150
GGDAKVIGVV AEDQRLDHIQ DIGDVPEGVK QEIQHFFETY KALEAKKGKW
160 170
VKVTGWRDRK AALEEVRACI ARYKG
Length:175
Mass (Da):19,216
Last modified:January 23, 2007 - v2
Checksum:i01306D30B31DB061
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010580 Genomic DNA. Translation: BAA24521.1.
AP008226 Genomic DNA. Translation: BAD71788.1.
RefSeqiWP_011173971.1. NC_006461.1.
YP_145231.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71788; BAD71788; BAD71788.
GeneIDi3169724.
KEGGittj:TTHA1965.
PATRICi23958909. VBITheThe93045_1936.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010580 Genomic DNA. Translation: BAA24521.1.
AP008226 Genomic DNA. Translation: BAD71788.1.
RefSeqiWP_011173971.1. NC_006461.1.
YP_145231.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PRDX-ray2.00A2-175[»]
ProteinModelPortaliP38576.
SMRiP38576. Positions 2-175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1965.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71788; BAD71788; BAD71788.
GeneIDi3169724.
KEGGittj:TTHA1965.
PATRICi23958909. VBITheThe93045_1936.

Phylogenomic databases

eggNOGiENOG4105F0N. Bacteria.
COG0221. LUCA.
HOGENOMiHOG000236472.
KOiK01507.
OMAiDGVANYK.
PhylomeDBiP38576.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-2015-MONOMER.
BRENDAi3.6.1.1. 2305.

Miscellaneous databases

EvolutionaryTraceiP38576.

Family and domain databases

CDDicd00412. pyrophosphatase. 1 hit.
Gene3Di3.90.80.10. 1 hit.
HAMAPiMF_00209. Inorganic_PPase. 1 hit.
InterProiIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERiPTHR10286. PTHR10286. 1 hit.
PfamiPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMiSSF50324. SSF50324. 1 hit.
PROSITEiPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPYR_THET8
AccessioniPrimary (citable) accession number: P38576
Secondary accession number(s): Q5SGW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.