Reviewed, UniProtKB/Swiss-Prot P38576 (IPYR_THET8)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	TTHA1965

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	175 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product. HAMAP MF_00209
Subunit structure	Homohexamer. HAMAP MF_00209
Subcellular location	Cytoplasm. HAMAP MF_00209
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed HAMAP MF_00209

Chain

2 – 175

174

Inorganic pyrophosphatase HAMAP MF_00209

PRO_0000137535

Sites

Metal binding

66

1

Magnesium 1 HAMAP MF_00209

Metal binding

71

1

Magnesium 1 HAMAP MF_00209

Metal binding

71

1

Magnesium 2 HAMAP MF_00209

Metal binding

103

1

Magnesium 1 HAMAP MF_00209

Secondary structure

1

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175

Helix Strand Turn

Details...

Helix

4 – 6

3

Turn

11 – 15

5

Beta strand

16 – 23

8

Beta strand

29 – 34

6

Turn

35 – 38

4

Beta strand

39 – 45

7

Beta strand

47 – 50

4

Beta strand

53 – 58

6

Beta strand

71 – 75

5

Beta strand

85 – 100

16

Beta strand

102 – 110

9

Helix

114 – 116

3

Helix

122 – 124

3

Helix

127 – 139

13

Helix

142 – 147

6

Beta strand

151 – 157

7

Helix

159 – 174

16

Sequences

	Sequence		Length	Mass (Da)
	P38576-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 01306D30B31DB061	FASTA	175	19,216
	10 20 30 40 50 60 MANLKSLPVG DKAPEVVHMV IEVPRGSGNK YEYDPDLGAI KLDRVLPGAQ FYPGDYGFIP 70 80 90 100 110 120 STLAEDGDPL DGLVLSTYPL LPGVVVEVRV VGLLLMEDEK GGDAKVIGVV AEDQRLDHIQ 130 140 150 160 170 DIGDVPEGVK QEIQHFFETY KALEAKKGKW VKVTGWRDRK AALEEVRACI ARYKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, expression, and site-directed mutagenesis of inorganic pyrophosphatase from Thermus thermophilus HB8." Satoh T., Samejima T., Watanabe M., Nogi S., Takahashi Y., Kaji H., Teplyakov A., Obmolova G., Kuranova I., Ishii K. J. Biochem. 124:79-88(1998) [PubMed: 9644249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Crystal structure of inorganic pyrophosphatase from Thermus thermophilus." Teplyakov A., Obmolova G., Wilson K.S., Ishii K., Kaji H., Samejima T., Kuranova I. Protein Sci. 3:1098-1107(1994) [PubMed: 7920256] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

AB010580 Genomic DNA. Translation: BAA24521.1.
AP008226 Genomic DNA. Translation: BAD71788.1.

RefSeq

YP_145231.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PRD	X-ray	2.00	A	2-175	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P38576.

Genome annotation databases

GeneID

3169724.

GenomeReviews

Gene locus TTHA1965 in contig AP008226_GR.

KEGG

ttj:TTHA1965.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P38576.

OMA

EIDKDTG.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA1965-MON.

Family and domain databases

HAMAP

MF_00209.
[Tree]

InterPro

IPR008162. Pyrophosphatase.
[Graphical view]

Gene3D

G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.

PANTHER

PTHR10286. Pyrophosphatase. 1 hit.

Pfam

PF00719. Pyrophosphatase. 1 hit.
[Graphical view]

ProDom

PD002014. Inorg_pphsph. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00387. PPASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IPYR_THET8

Accession

Primary (citable) accession number: P38576
Secondary accession number(s): Q5SGW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)