Reviewed, UniProtKB/Swiss-Prot P38570 (ITAE_HUMAN)

Names and origin

Protein names

Recommended name:
    Integrin alpha-E
Alternative name(s):
    Mucosal lymphocyte 1 antigen
    HML-1 antigen
    Integrin alpha-IEL
    CD_antigen=CD103
Cleaved into the following 2 chains:
    1- Recommended name:
            Integrin alpha-E light chain
    2- Recommended name:
            Integrin alpha-E heavy chain

Gene names

Name:

ITGAE

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
Subunit structure	Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chains linked by a disulfide bond. Alpha-E associates with beta-7.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
Induction	Integrin alpha-E/beta-7 is induced by TGFB1. Ref.6
Domain	The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Sequence similarities	Belongs to the integrin alpha chain family. Contains 5 FG-GAP repeats. Contains 1 VWFA domain.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Signal Transmembrane
Ligand	Calcium Magnesium
Molecular function	Integrin Receptor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW integrin-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integrin complex Ref.1 Traceable author statement. Source: ProtInc
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: UniProtKB-KW receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.1
	Chain	19 – 1179	1161	Integrin alpha-E		PRO_0000016283
	Chain	19 – 177	159	Integrin alpha-E light chain		PRO_0000016284
	Chain	179 – 1177	999	Integrin alpha-E heavy chain		PRO_0000016285
Regions
	Topological domain	19 – 1124	1106	Extracellular Potential
	Transmembrane	1125 – 1147	23	Potential
	Topological domain	1148 – 1179	32	Cytoplasmic Potential
	Domain	200 – 391	192	VWFA
	Repeat	401 – 456	56	FG-GAP 1
	Repeat	457 – 506	50	FG-GAP 2
	Repeat	510 – 571	62	FG-GAP 3
	Repeat	573 – 638	66	FG-GAP 4
	Repeat	641 – 693	53	FG-GAP 5
	Calcium binding	522 – 530	9	Potential
	Calcium binding	586 – 594	9	Potential
	Calcium binding	654 – 662	9	Potential
	Region	145 – 199	55	X-domain (extra domain)
	Motif	1150 – 1154	5	GFFKR motif
	Compositional bias	181 – 198	18	Glu-rich (acidic)
Amino acid modifications
	Glycosylation	49	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	321	1	N-linked (GlcNAc...) Potential
	Glycosylation	444	1	N-linked (GlcNAc...) Potential
	Glycosylation	726	1	N-linked (GlcNAc...) Potential
	Glycosylation	782	1	N-linked (GlcNAc...) Potential
	Glycosylation	857	1	N-linked (GlcNAc...) Potential
	Glycosylation	934	1	N-linked (GlcNAc...) Potential
	Glycosylation	954	1	N-linked (GlcNAc...) Potential
	Glycosylation	1065	1	N-linked (GlcNAc...) Potential
	Glycosylation	1096	1	N-linked (GlcNAc...) Potential
	Disulfide bond	70 ↔ 79		By similarity
	Disulfide bond	126 ↔ 159		By similarity
	Disulfide bond	706 ↔ 762		By similarity
	Disulfide bond	823 ↔ 829		By similarity
	Disulfide bond	893 ↔ 907		By similarity
	Disulfide bond	1008 ↔ 1033		By similarity
	Disulfide bond	1041 ↔ 1057		By similarity
Natural variations
	Natural variant	360	1	D → E		VAR_008884
	Natural variant	477	1	I → V: dbSNP rs220479. Ref.1 Ref.4		VAR_054889
	Natural variant	482	1	R → Q: dbSNP rs2272606. Ref.1 Ref.4		VAR_054890
	Natural variant	892	1	Q → H: dbSNP rs3744679.		VAR_020037
	Natural variant	950	1	R → W: dbSNP rs1716. Ref.5		VAR_034025
	Natural variant	1019	1	V → A: dbSNP rs2976230. Ref.1 Ref.4		VAR_054891
	Natural variant	1041	1	C → S		VAR_008885
Experimental info
	Mutagenesis	208	1	D → A: Loss of E-cadherin binding. Ref.7
	Mutagenesis	316	1	F → A: Loss of E-cadherin binding. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P38570-1 [UniParc]. Last modified April 14, 2009. Version 3. Checksum: 6645F8FFF9A0F045	FASTA	1,179	130,159
	10 20 30 40 50 60 MWLFHTLLCI ASLALLAAFN VDVARPWLTP KGGAPFVLSS LLHQDPSTNQ TWLLVTSPRT 70 80 90 100 110 120 KRTPGPLHRC SLVQDEILCH PVEHVPIPKG RHRGVTVVRS HHGVLICIQV LVRRPHSLSS 130 140 150 160 170 180 ELTGTCSLLG PDLRPQAQAN FFDLENLLDP DARVDTGDCY SNKEGGGEDD VNTARQRRAL 190 200 210 220 230 240 EKEEEEDKEE EEDEEEEEAG TEIAIILDGS GSIDPPDFQR AKDFISNMMR NFYEKCFECN 250 260 270 280 290 300 FALVQYGGVI QTEFDLRDSQ DVMASLARVQ NITQVGSVTK TASAMQHVLD SIFTSSHGSR 310 320 330 340 350 360 RKASKVMVVL TDGGIFEDPL NLTTVINSPK MQGVERFAIG VGEEFKSART ARELNLIASD 370 380 390 400 410 420 PDETHAFKVT NYMALDGLLS KLRYNIISME GTVGDALHYQ LAQIGFSAQI LDERQVLLGA 430 440 450 460 470 480 VGAFDWSGGA LLYDTRSRRG RFLNQTAAAA ADAEAAQYSY LGYAVAVLHK TCSLSYIAGA 490 500 510 520 530 540 PRYKHHGAVF ELQKEGREAS FLPVLEGEQM GSYFGSELCP VDIDMDGSTD FLLVAAPFYH 550 560 570 580 590 600 VHGEEGRVYV YRLSEQDGSF SLARILSGHP GFTNARFGFA MAAMGDLSQD KLTDVAIGAP 610 620 630 640 650 660 LEGFGADDGA SFGSVYIYNG HWDGLSASPS QRIRASTVAP GLQYFGMSMA GGFDISGDGL 670 680 690 700 710 720 ADITVGTLGQ AVVFRSRPVV RLKVSMAFTP SALPIGFNGV VNVRLCFEIS SVTTASESGL 730 740 750 760 770 780 REALLNFTLD VDVGKQRRRL QCSDVRSCLG CLREWSSGSQ LCEDLLLMPT EGELCEEDCF 790 800 810 820 830 840 SNASVKVSYQ LQTPEGQTDH PQPILDRYTE PFAIFQLPYE KACKNKLFCV AELQLATTVS 850 860 870 880 890 900 QQELVVGLTK ELTLNINLTN SGEDSYMTSM ALNYPRNLQL KRMQKPPSPN IQCDDPQPVA 910 920 930 940 950 960 SVLIMNCRIG HPVLKRSSAH VSVVWQLEEN AFPNRTADIT VTVTNSNERR SLANETHTLQ 970 980 990 1000 1010 1020 FRHGFVAVLS KPSIMYVNTG QGLSHHKEFL FHVHGENLFG AEYQLQICVP TKLRGLQVVA 1030 1040 1050 1060 1070 1080 VKKLTRTQAS TVCTWSQERA CAYSSVQHVE EWHSVSCVIA SDKENVTVAA EISWDHSEEL 1090 1100 1110 1120 1130 1140 LKDVTELQIL GEISFNKSLY EGLNAENHRT KITVVFLKDE KYHSLPIIIK GSVGGLLVLI 1150 1160 1170 VILVILFKCG FFKRKYQQLN LESIRKAQLK SENLLEEEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the human mucosal lymphocyte integrin alpha E subunit. Unusual structure and restricted RNA distribution." Shaw S.K., Cepek K.L., Murphy E.A., Russell G.J., Brenner M.B., Parker C.M. J. Biol. Chem. 269:6016-6025(1994) [PubMed: 8119947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-38 AND 179-188, VARIANTS VAL-477; GLN-482 AND ALA-1019. Tissue: Leukemia and Lymphocyte.
[2]	Parker C.M. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 88-114.
[3]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed: 16625196] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS VAL-477; GLN-482 AND ALA-1019.
[5]	"The genomic region encompassing the nephropathic cystinosis gene (CTNS): complete sequencing of a 200-kb segment and discovery of a novel gene within the common cystinosis-causing deletion." Touchman J.W., Anikster Y., Dietrich N.L., Maduro V.V.B., McDowell G., Shotelersuk V., Bouffard G.G., Beckstrom-Sternberg S.M., Gahl W.A., Green E.D. Genome Res. 10:165-173(2000) [PubMed: 10673275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 53-1179, VARIANT TRP-950. Tissue: Fetal kidney.
[6]	"A family of beta 7 integrins on human mucosal lymphocytes." Parker C.M., Cepek K.L., Russell G.J., Shaw S.K., Posnett D.N., Schwarting R., Brenner M.B. Proc. Natl. Acad. Sci. U.S.A. 89:1924-1928(1992) [PubMed: 1542691] [Abstract] Cited for: INTERACTION WITH INTEGRIN BETA-7, INDUCTION.
[7]	"The role of alpha and beta chains in ligand recognition by beta 7 integrins." Higgins J.M.G., Cernadas M., Tan K., Irie A., Wang J.-H., Takada Y., Brenner M.B. J. Biol. Chem. 275:25652-25664(2000) [PubMed: 10837471] [Abstract] Cited for: MUTAGENESIS OF ASP-208 AND PHE-316.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L25851 mRNA. Translation: AAB59359.2. AC116914 Genomic DNA. No translation available. BC113436 mRNA. Translation: AAI13437.1. BC117207 mRNA. Translation: AAI17208.1. AF168787 Genomic DNA. Translation: AAF43107.1.
IPI	IPI00007205.
PIR	A53213.
RefSeq	NP_002199.3.
UniGene	Hs.513867
3D structure databases
HSSP	HSSP built from PDB template 1BHQ based on UniProtKB P11215.
ModBase	Search...
Protein-protein interaction databases
IntAct	P38570. 1 interaction.
Proteomic databases
PRIDE	P38570.
Genome annotation databases
Ensembl	ENSG00000083457. Homo sapiens. [Contig view]
GeneID	3682.
KEGG	hsa:3682.
Organism-specific databases
GeneCards	GC17M003564.
H-InvDB	HIX0013432.
HGNC	HGNC:6147. ITGAE.
MIM	604682. gene.
PharmGKB	PA29947.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P38570.
HOVERGEN	P38570.
OMA	P38570. ELTGTCS.
Enzyme and pathway databases
Reactome	REACT_13552. Integrin cell surface interactions.
Gene expression databases
ArrayExpress	P38570.
Bgee	P38570.
CleanEx	HS_ITGAE.
GermOnline	ENSG00000083457. Homo sapiens.
Family and domain databases
InterPro	IPR013517. FG-GAP. IPR013519. Int_alpha_beta-p. IPR000413. Integrin_alpha. IPR013649. Integrin_alpha-2. IPR013513. Integrin_alpha_C. IPR018184. Integrin_alpha_C_CS. IPR002035. VWF_A. [Graphical view]
Pfam	PF01839. FG-GAP. 2 hits. PF00357. Integrin_alpha. 1 hit. PF08441. Integrin_alpha2. 1 hit. PF00092. VWA. 1 hit. [Graphical view]
PRINTS	PR01185. INTEGRINA.
SMART	SM00191. Int_alpha. 4 hits. SM00327. VWA. 1 hit. [Graphical view]
PROSITE	PS00242. INTEGRIN_ALPHA. 1 hit. PS50234. VWFA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	14411.
SOURCE	Search...

Entry information

Entry name

ITAE_HUMAN

Accession

Primary (citable) accession number: P38570
Secondary accession number(s): Q17RS6, Q9NZU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	April 14, 2009
Last modified:	June 16, 2009
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)