Reviewed, UniProtKB/Swiss-Prot P38568 (HYALP_MACFA)

Names and origin

Protein names

Recommended name:
    Hyaluronidase PH-20
      Short name=Hyal-PH20
    EC=3.2.1.35
Alternative name(s):
    Hyaluronoglucosaminidase PH-20
    Sperm surface protein PH-20
    Sperm adhesion molecule 1

Gene names

Name:	SPAM1
Synonyms:	PH20

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Testis.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW fusion of sperm to egg plasma membrane Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	By similarity
	Chain	36 – 491	456	Hyaluronidase PH-20		PRO_0000012091
	Propeptide	492 – 510	19	Removed in mature form Potential		PRO_0000012092
Sites
	Active site	148	1	Proton donor By similarity
Amino acid modifications
	Lipidation	491	1	GPI-anchor amidated serine Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Glycosylation	235	1	N-linked (GlcNAc...) Potential
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	368	1	N-linked (GlcNAc...) Potential
	Glycosylation	393	1	N-linked (GlcNAc...) Potential
	Glycosylation	440	1	N-linked (GlcNAc...) Potential
	Glycosylation	484	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 351		By similarity
	Disulfide bond	224 ↔ 238		By similarity
	Disulfide bond	376 ↔ 387		By similarity
	Disulfide bond	381 ↔ 435		By similarity
	Disulfide bond	437 ↔ 464		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38568-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: D50EE36C67CF1BBF	FASTA	510	57,935
	10 20 30 40 50 60 MGVLKFKHIF FRSFVKSSGV SQIVFTFLLI PCCLTLNFRA PPIIPNVPFL WAWNAPSEFC 70 80 90 100 110 120 LGKFNEPLDM SLFTLMGSPR INVTGQGVTI FYVDRLGYYP YIDLTTGVTV HGGIPQKVSL 130 140 150 160 170 180 QDHLDKSKQD ILFYMPVDNL GMAVIDWEEW RPTWARNWKP KDVYKNRSIE LVQQQNVQLS 190 200 210 220 230 240 LPQATDKAKQ EFEKAGKDFM LETIKLGRSL RPNHLWGYYL FPDCYNHHYR KPGYNGSCFD 250 260 270 280 290 300 VEIKRNDDLS WLWNESTALY PSIYLNTQQS VVVATLYVRN RVREAIRVSK IPDAKNPLPV 310 320 330 340 350 360 FVYARLVFTD QVLKFLSREE LVSTLGETVA LGASGIVIWG SLSITRSMKS CLLLDTYMET 370 380 390 400 410 420 ILNPYIINVT LAAKMCSQVL CQEQGVCIRK DWNSSDYLHL NPDNFDIRLE KGGKFTVHGK 430 440 450 460 470 480 PTVEDLEEFS EKFYCSCYTN LSCKEKADVK DTDAVDVCIA DGVCIDASLK PPVETEGSPP 490 500 510 IFYNTSSSTV STTMFIVNIL FLIISSVASL

References

[1]	"Molecular cloning of the human and monkey sperm surface protein PH-20." Lin Y., Kimmel L.H., Myles D.G., Primakoff P. Proc. Natl. Acad. Sci. U.S.A. 90:10071-10075(1993) [PubMed: 8234258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L13780 mRNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1FCQ based on UniProtKB Q08169.
ModBase	Search...
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Phylogenomic databases
HOVERGEN	P38568.
Enzyme and pathway databases
BRENDA	3.2.1.35. 3438.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR017430. Glyco_hydro_56_Hyaluronidase. IPR001439. Glyco_hydro_56_PH20. IPR001968. Glycoside_hydrolase_family_56. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit. PTHR11769:SF4. Glyco_hydro_56_PH20. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56. PR00848. SPERMPH20.
ProDom	PD003549. Glyco_hydro_56. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

HYALP_MACFA

Accession

Primary (citable) accession number: P38568

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)