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Protein

Hyaluronidase PH-20

Gene

SPAM1

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei148Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell adhesion

Protein family/group databases

CAZyiGH56 Glycoside Hydrolase Family 56

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase PH-20 (EC:3.2.1.35)
Short name:
Hyal-PH20
Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface protein PH-20
Gene namesi
Name:SPAM1
Synonyms:PH20
ORF Names:EGM_12920
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
Proteomesi
  • UP000009130 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35By similarityAdd BLAST35
ChainiPRO_000001209136 – 491Hyaluronidase PH-20Add BLAST456
PropeptideiPRO_0000012092492 – 510Removed in mature formSequence analysisAdd BLAST19

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 351By similarity
Glycosylationi82N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi224 ↔ 238By similarity
Glycosylationi235N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi254N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi368N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi376 ↔ 387By similarity
Disulfide bondi381 ↔ 435By similarity
Glycosylationi393N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi437 ↔ 464By similarity
Glycosylationi440N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi484N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi491GPI-anchor amidated serineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiP38568

Expressioni

Tissue specificityi

Testis.

Structurei

3D structure databases

ProteinModelPortaliP38568
SMRiP38568
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052053

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR018155 Hyaluronidase
IPR001439 Hyaluronidase_PH20/Hyal5
PANTHERiPTHR11769 PTHR11769, 1 hit
PfamiView protein in Pfam
PF01630 Glyco_hydro_56, 1 hit
PIRSFiPIRSF038193 Hyaluronidase, 1 hit
PIRSF500773 Hyaluronidase_PH20_Hyal5, 1 hit
PRINTSiPR00846 GLHYDRLASE56
SUPFAMiSSF51445 SSF51445, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P38568-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVLKFKHIF FRSFVKSSGV SQIVFTFLLI PCCLTLNFRA PPIIPNVPFL
60 70 80 90 100
WAWNAPSEFC LGKFNEPLDM SLFTLMGSPR INVTGQGVTI FYVDRLGYYP
110 120 130 140 150
YIDLTTGVTV HGGIPQKVSL QDHLDKSKQD ILFYMPVDNL GMAVIDWEEW
160 170 180 190 200
RPTWARNWKP KDVYKNRSIE LVQQQNVQLS LPQATDKAKQ EFEKAGKDFM
210 220 230 240 250
LETIKLGRSL RPNHLWGYYL FPDCYNHHYR KPGYNGSCFD VEIKRNDDLS
260 270 280 290 300
WLWNESTALY PSIYLNTQQS VVVATLYVRN RVREAIRVSK IPDAKNPLPV
310 320 330 340 350
FVYARLVFTD QVLKFLSREE LVSTLGETVA LGASGIVIWG SLSITRSMKS
360 370 380 390 400
CLLLDTYMET ILNPYIINVT LAAKMCSQVL CQEQGVCIRK DWNSSDYLHL
410 420 430 440 450
NPDNFDIRLE KGGKFTVHGK PTVEDLEEFS EKFYCSCYTN LSCKEKADVK
460 470 480 490 500
DTDAVDVCIA DGVCIDASLK PPVETEGSPP IFYNTSSSTV STTMFIVNIL
510
FLIISSVASL
Length:510
Mass (Da):57,935
Last modified:October 1, 1994 - v1
Checksum:iD50EE36C67CF1BBF
GO
Isoform 2 (identifier: P38568-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     497-510: VNILFLIISSVASL → WRLEVWDQGISRIGFF

Note: No experimental confirmation available.
Show »
Length:512
Mass (Da):58,455
Checksum:iBC638EE841D2485F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_046430497 – 510VNILF…SVASL → WRLEVWDQGISRIGFF in isoform 2. CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13780 mRNA No translation available.
CM001278 Genomic DNA Translation: EHH52472.1
UniGeneiMfa.358

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHYALP_MACFA
AccessioniPrimary (citable) accession number: P38568
Secondary accession number(s): G7P0K2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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