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P38568

- HYALP_MACFA

UniProt

P38568 - HYALP_MACFA

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Protein

Hyaluronidase PH-20

Gene

SPAM1

Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei148 – 1481Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell adhesion Source: UniProtKB-KW
  3. fusion of sperm to egg plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell adhesion

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase PH-20 (EC:3.2.1.35)
Short name:
Hyal-PH20
Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface protein PH-20
Gene namesi
Name:SPAM1
Synonyms:PH20
ORF Names:EGM_12920
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
ProteomesiUP000009130: Chromosome 3

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535By similarityAdd
BLAST
Chaini36 – 491456Hyaluronidase PH-20PRO_0000012091Add
BLAST
Propeptidei492 – 51019Removed in mature formSequence AnalysisPRO_0000012092Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi60 ↔ 351By similarity
Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi224 ↔ 238By similarity
Glycosylationi235 – 2351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi376 ↔ 387By similarity
Disulfide bondi381 ↔ 435By similarity
Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi437 ↔ 464By similarity
Glycosylationi440 – 4401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi484 – 4841N-linked (GlcNAc...)Sequence Analysis
Lipidationi491 – 4911GPI-anchor amidated serineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Tissue specificityi

Testis.

Structurei

3D structure databases

ProteinModelPortaliP38568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052053.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P38568-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGVLKFKHIF FRSFVKSSGV SQIVFTFLLI PCCLTLNFRA PPIIPNVPFL
60 70 80 90 100
WAWNAPSEFC LGKFNEPLDM SLFTLMGSPR INVTGQGVTI FYVDRLGYYP
110 120 130 140 150
YIDLTTGVTV HGGIPQKVSL QDHLDKSKQD ILFYMPVDNL GMAVIDWEEW
160 170 180 190 200
RPTWARNWKP KDVYKNRSIE LVQQQNVQLS LPQATDKAKQ EFEKAGKDFM
210 220 230 240 250
LETIKLGRSL RPNHLWGYYL FPDCYNHHYR KPGYNGSCFD VEIKRNDDLS
260 270 280 290 300
WLWNESTALY PSIYLNTQQS VVVATLYVRN RVREAIRVSK IPDAKNPLPV
310 320 330 340 350
FVYARLVFTD QVLKFLSREE LVSTLGETVA LGASGIVIWG SLSITRSMKS
360 370 380 390 400
CLLLDTYMET ILNPYIINVT LAAKMCSQVL CQEQGVCIRK DWNSSDYLHL
410 420 430 440 450
NPDNFDIRLE KGGKFTVHGK PTVEDLEEFS EKFYCSCYTN LSCKEKADVK
460 470 480 490 500
DTDAVDVCIA DGVCIDASLK PPVETEGSPP IFYNTSSSTV STTMFIVNIL
510
FLIISSVASL
Length:510
Mass (Da):57,935
Last modified:October 1, 1994 - v1
Checksum:iD50EE36C67CF1BBF
GO
Isoform 2 (identifier: P38568-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     497-510: VNILFLIISSVASL → WRLEVWDQGISRIGFF

Note: No experimental confirmation available.

Show »
Length:512
Mass (Da):58,455
Checksum:iBC638EE841D2485F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei497 – 51014VNILF…SVASL → WRLEVWDQGISRIGFF in isoform 2. CuratedVSP_046430Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L13780 mRNA. No translation available.
CM001278 Genomic DNA. Translation: EHH52472.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L13780 mRNA. No translation available.
CM001278 Genomic DNA. Translation: EHH52472.1 .

3D structure databases

ProteinModelPortali P38568.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG052053.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view ]
PANTHERi PTHR11769. PTHR11769. 1 hit.
Pfami PF01630. Glyco_hydro_56. 1 hit.
[Graphical view ]
PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSi PR00846. GLHYDRLASE56.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of the human and monkey sperm surface protein PH-20."
    Lin Y., Kimmel L.H., Myles D.G., Primakoff P.
    Proc. Natl. Acad. Sci. U.S.A. 90:10071-10075(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiHYALP_MACFA
AccessioniPrimary (citable) accession number: P38568
Secondary accession number(s): G7P0K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3