P38566 (HYALP_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronidase PH-20
Short name=Hyal-PH20
EC=3.2.1.35

Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface protein PH-20

Gene names

Name:	SPAM1
Synonyms:	PH20

Organism

Oryctolagus cuniculus (Rabbit) [Complete proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor.
Tissue specificity	Testis.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW fusion of sperm to egg plasma membrane Inferred from electronic annotation. Source: InterPro
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	By similarity
	Chain	36 – ?		Hyaluronidase PH-20		PRO_0000012095
	Propeptide	? – 545		Removed in mature form Potential		PRO_0000012096
Sites
	Active site	148	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
	Glycosylation	372	1	N-linked (GlcNAc...) Potential
	Disulfide bond	60 ↔ 355		By similarity
	Disulfide bond	224 ↔ 239		By similarity
	Disulfide bond	380 ↔ 391		By similarity
	Disulfide bond	385 ↔ 439		By similarity
	Disulfide bond	441 ↔ 468		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38566 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 125AF314FE05AEB4	FASTA	545	62,412
	10 20 30 40 50 60 MGVLKFKHIF FGSAVELSGV FQIVFIFLLI PCCLTANFRA PPVIPNVPFL WAWNAPTEFC 70 80 90 100 110 120 LGKSGEPLDM SLFSLFGSPR KNKTGQGITI FYVDRLGYYP YIDPHTGAIV HGRIPQLGPL 130 140 150 160 170 180 QQHLTKLRQE ILYYMPKDNV GLAVIDWEEW LPTWLRNWKP KDIYRIKSIE LVKSQHPQYN 190 200 210 220 230 240 HSYATEKAKR DFEKAGKDFM EETLKLGRLL RPNHLWGYYL FPDCYNHHYD KPNLYKGSCF 250 260 270 280 290 300 DIEKKRNDDL SWLWKESTAL FPSVYLTSRA RSATALSKLY VVRNRVHEAI RVSKIPDDKS 310 320 330 340 350 360 PLPNFVYTRL VFTDQIFQFL SHHDLVYTIG EIVALGASGI VVWGSQSLAR SMKSCLHLDN 370 380 390 400 410 420 YMKTILNPYL INVTLAAKMC NQVLCQEQGV CTRKNWNPND YLHLNPGNFA IQLGSNGTYK 430 440 450 460 470 480 VDGKPTLTDL EQFSKNFQCS CYTNLNCKER TDMNNVRTVN VCAVENVCID TNVGPQAVTY 490 500 510 520 530 540 APKEKKDVAH ILSNTTSINS STTMSLPFPR KHVSGCLLVL CMYSQYLNIC YRLVAIGIQH GYYLK

References

[1]	"The nucleic acid sequence of rabbit PH-20 cDNA." Andrews J.B., Holland M.K. Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09183 mRNA. Translation: AAA88913.1.
RefSeq	NP_001076141.1. NM_001082672.1.
UniGene	Ocu.2048.
3D structure databases
ProteinModelPortal	P38566.
ModBase	Search...
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009391.
Organism-specific databases
CTD	6677.
Phylogenomic databases
eggNOG	maNOG04106.
HOVERGEN	HBG052053.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR001439. Glyco_hydro_56_PH20. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit. PTHR11769:SF4. Glyco_hydro_56_PH20. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56. PR00848. SPERMPH20.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HYALP_RABIT

Accession

Primary (citable) accession number: P38566

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1996
Last modified:	October 19, 2011
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program