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P38566

- HYALP_RABIT

UniProt

P38566 - HYALP_RABIT

Protein

Hyaluronidase PH-20

Gene

SPAM1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei148 – 1481Proton donorBy similarity

    GO - Molecular functioni

    1. hyalurononglucosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cell adhesion Source: UniProtKB-KW
    3. fusion of sperm to egg plasma membrane Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell adhesion

    Protein family/group databases

    CAZyiGH56. Glycoside Hydrolase Family 56.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronidase PH-20 (EC:3.2.1.35)
    Short name:
    Hyal-PH20
    Alternative name(s):
    Hyaluronoglucosaminidase PH-20
    Sperm adhesion molecule 1
    Sperm surface protein PH-20
    Gene namesi
    Name:SPAM1
    Synonyms:PH20
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei? – 545Removed in mature formSequence AnalysisPRO_0000012096
    Signal peptidei1 – 3535By similarityAdd
    BLAST
    Chaini36 – ?Hyaluronidase PH-20PRO_0000012095

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi60 ↔ 355By similarity
    Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi180 – 1801N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi224 ↔ 239By similarity
    Glycosylationi372 – 3721N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi380 ↔ 391By similarity
    Disulfide bondi385 ↔ 439By similarity
    Disulfide bondi441 ↔ 468By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Expressioni

    Tissue specificityi

    Testis.

    Structurei

    3D structure databases

    ProteinModelPortaliP38566.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 56 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG052053.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    IPR001439. Hyaluronidase_PH20.
    [Graphical view]
    PANTHERiPTHR11769. PTHR11769. 1 hit.
    PfamiPF01630. Glyco_hydro_56. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
    PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
    PRINTSiPR00846. GLHYDRLASE56.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVLKFKHIF FGSAVELSGV FQIVFIFLLI PCCLTANFRA PPVIPNVPFL    50
    WAWNAPTEFC LGKSGEPLDM SLFSLFGSPR KNKTGQGITI FYVDRLGYYP 100
    YIDPHTGAIV HGRIPQLGPL QQHLTKLRQE ILYYMPKDNV GLAVIDWEEW 150
    LPTWLRNWKP KDIYRIKSIE LVKSQHPQYN HSYATEKAKR DFEKAGKDFM 200
    EETLKLGRLL RPNHLWGYYL FPDCYNHHYD KPNLYKGSCF DIEKKRNDDL 250
    SWLWKESTAL FPSVYLTSRA RSATALSKLY VVRNRVHEAI RVSKIPDDKS 300
    PLPNFVYTRL VFTDQIFQFL SHHDLVYTIG EIVALGASGI VVWGSQSLAR 350
    SMKSCLHLDN YMKTILNPYL INVTLAAKMC NQVLCQEQGV CTRKNWNPND 400
    YLHLNPGNFA IQLGSNGTYK VDGKPTLTDL EQFSKNFQCS CYTNLNCKER 450
    TDMNNVRTVN VCAVENVCID TNVGPQAVTY APKEKKDVAH ILSNTTSINS 500
    STTMSLPFPR KHVSGCLLVL CMYSQYLNIC YRLVAIGIQH GYYLK 545
    Length:545
    Mass (Da):62,412
    Last modified:October 1, 1996 - v2
    Checksum:i125AF314FE05AEB4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09183 mRNA. Translation: AAA88913.1.
    RefSeqiNP_001076141.1. NM_001082672.1.
    UniGeneiOcu.2048.

    Genome annotation databases

    GeneIDi100009391.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09183 mRNA. Translation: AAA88913.1 .
    RefSeqi NP_001076141.1. NM_001082672.1.
    UniGenei Ocu.2048.

    3D structure databases

    ProteinModelPortali P38566.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH56. Glycoside Hydrolase Family 56.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009391.

    Organism-specific databases

    CTDi 6677.

    Phylogenomic databases

    HOVERGENi HBG052053.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    IPR001439. Hyaluronidase_PH20.
    [Graphical view ]
    PANTHERi PTHR11769. PTHR11769. 1 hit.
    Pfami PF01630. Glyco_hydro_56. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
    PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
    PRINTSi PR00846. GLHYDRLASE56.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleic acid sequence of rabbit PH-20 cDNA."
      Andrews J.B., Holland M.K.
      Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiHYALP_RABIT
    AccessioniPrimary (citable) accession number: P38566
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3