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Protein

Glutamine synthetase root isozyme 5

Gene

GS1-5

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in the flow of nitrogen into nitrogenous organic compounds.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase root isozyme 5 (EC:6.3.1.2)
Alternative name(s):
GS117
Glutamate--ammonia ligase
Gene namesi
Name:GS1-5
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305 Componenti: Unplaced

Organism-specific databases

GrameneiP38563.
MaizeGDBi17151.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Glutamine synthetase root isozyme 5PRO_0000153182Add
BLAST

Proteomic databases

PRIDEiP38563.

Expressioni

Tissue specificityi

Found mainly in the cortical tissues of seedling roots, stem and seedling shoot.

Gene expression databases

ExpressionAtlasiP38563. baseline and differential.

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP38563.
SMRiP38563. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLTDLVNL DLSDCTDKII AEYIWVGGSG IDLRSKARTV KGPITDPSQL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRKGNNILV MCDCYTPQGE
110 120 130 140 150
PIPSNKRYKA ATVFSHPDVA AEVPWYGIEQ EYTLLQKDLS WPLGWPVGGY
160 170 180 190 200
PGPQGPYYCA AGADKAFGRD VVDAHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISA GDEIWVARYI LERITEMAGI VLSLDPKPIK GDWNGAGAHT
260 270 280 290 300
NYSTKSMREA GGYEVIKEAI EKLGRRHREH IAAYGEGNER RLTGRHETAD
310 320 330 340 350
INTFKWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTGMIADT

TILWKGN
Length:357
Mass (Da):39,259
Last modified:October 31, 1995 - v2
Checksum:i183F9C15F2FF33A6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti139 – 1391L → V in CAA46723 (PubMed:8106013).Curated
Sequence conflicti275 – 2751R → K in CAA46723 (PubMed:8106013).Curated
Sequence conflicti285 – 2862GE → DG in CAA46723 (PubMed:8106013).Curated
Sequence conflicti313 – 3131A → G in CAA46723 (PubMed:8106013).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14578 mRNA. Translation: BAA03432.1. Sequence problems.
X65930 mRNA. Translation: CAA46723.1.
PIRiS39481.
RefSeqiNP_001105297.1. NM_001111827.1.
UniGeneiZm.15859.

Genome annotation databases

GeneIDi542215.
KEGGizma:542215.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14578 mRNA. Translation: BAA03432.1. Sequence problems.
X65930 mRNA. Translation: CAA46723.1.
PIRiS39481.
RefSeqiNP_001105297.1. NM_001111827.1.
UniGeneiZm.15859.

3D structure databases

ProteinModelPortaliP38563.
SMRiP38563. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP38563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi542215.
KEGGizma:542215.

Organism-specific databases

GrameneiP38563.
MaizeGDBi17151.

Phylogenomic databases

KOiK01915.

Gene expression databases

ExpressionAtlasiP38563. baseline and differential.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of the family of glutamine synthetase genes from maize: expression of genes for glutamine synthetase and ferredoxin-dependent glutamate synthase in photosynthetic and non-photosynthetic tissues."
    Sakakibara H., Kawabata S., Takahashi H., Hase T., Sugiyama T.
    Plant Cell Physiol. 33:49-58(1991)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Golden cross Bantam T51.
    Tissue: Leaf.
  2. "Differential expression of six glutamine synthetase genes in Zea mays."
    Li M.-G., Villemur R., Hussey P.J., Silflow C.D., Gantt J.S., Snustad D.P.
    Plant Mol. Biol. 23:401-407(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 113-357.
    Strain: cv. A188.
    Tissue: Seedling.

Entry informationi

Entry nameiGLNA5_MAIZE
AccessioniPrimary (citable) accession number: P38563
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1994
Last sequence update: October 31, 1995
Last modified: March 3, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.