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P38561

- GLNA3_MAIZE

UniProt

P38561 - GLNA3_MAIZE

Protein

Glutamine synthetase root isozyme 3

Gene

GLN4

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Plays a role in the flow of nitrogen into nitrogenous organic compounds.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. copper ion binding Source: EnsemblPlants/Gramene
    3. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. leaf senescence Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase root isozyme 3 (EC:6.3.1.2)
    Alternative name(s):
    GS112
    Glutamate--ammonia ligase
    Gene namesi
    Name:GLN4
    Synonyms:GS1-3
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiP38561.
    MaizeGDBi17151.

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall Source: EnsemblPlants/Gramene
    2. cytosolic ribosome Source: EnsemblPlants/Gramene
    3. plasma membrane Source: EnsemblPlants/Gramene
    4. plasmodesma Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Glutamine synthetase root isozyme 3PRO_0000153180Add
    BLAST

    Proteomic databases

    PRIDEiP38561.

    Expressioni

    Tissue specificityi

    Found in all the tissues examined with higher expression found in tissues of the root.

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    Secondary structure

    1
    356
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 85
    Helixi12 – 143
    Beta strandi19 – 268
    Beta strandi33 – 4210
    Helixi47 – 493
    Beta strandi53 – 564
    Turni57 – 615
    Beta strandi65 – 673
    Beta strandi69 – 7911
    Turni81 – 833
    Beta strandi88 – 958
    Helixi107 – 1159
    Helixi117 – 1226
    Beta strandi125 – 13612
    Turni137 – 1393
    Beta strandi154 – 1563
    Turni163 – 1653
    Helixi169 – 18214
    Beta strandi186 – 1916
    Beta strandi197 – 20610
    Helixi208 – 22821
    Beta strandi230 – 2334
    Beta strandi236 – 2394
    Beta strandi241 – 2433
    Beta strandi247 – 2537
    Turni255 – 2573
    Helixi262 – 27514
    Helixi277 – 2815
    Helixi288 – 2914
    Beta strandi293 – 2975
    Beta strandi306 – 3094
    Beta strandi313 – 3175
    Helixi319 – 3235
    Beta strandi329 – 3313
    Helixi340 – 35112

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2D3AX-ray2.63A/B/C/D/E/F/G/H/I/J1-356[»]
    2D3BX-ray3.50A/B/C/D/E/F/G/H/I/J1-356[»]
    2D3CX-ray3.81A/B/C/D/E/F/G/H/I/J1-356[»]
    ProteinModelPortaliP38561.
    SMRiP38561. Positions 3-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38561.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    HOGENOMiHOG000061500.
    KOiK01915.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38561-1 [UniParc]FASTAAdd to Basket

    « Hide

    MACLTDLVNL NLSDTTEKII AEYIWIGGSG MDLRSKARTL SGPVTDPSKL    50
    PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDCYTPAGE 100
    PIPTNKRYNA AKIFSSPEVA AEEPWYGIEQ EYTLLQKDTN WPLGWPIGGF 150
    PGPQGPYYCG IGAEKSFGRD IVDAHYKACL YAGINISGIN GEVMPGQWEF 200
    QVGPSVGISS GDQVWVARYI LERITEIAGV VVTFDPKPIP GDWNGAGAHT 250
    NYSTESMRKE GGYEVIKAAI EKLKLRHREH IAAYGEGNDG RLTGRHETAD 300
    INTFSWGVAN RGASVRVGRE TEQNGKGYFE DRRPASNMDP YVVTSMIAET 350
    TIIWKP 356
    Length:356
    Mass (Da):39,169
    Last modified:October 1, 1994 - v1
    Checksum:i0112DD32D5FB474C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151T → N in BAA03431. 1 PublicationCurated
    Sequence conflicti289 – 2902DG → ER in BAA03431. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65928 mRNA. Translation: CAA46721.1.
    D14577 mRNA. Translation: BAA03431.1.
    PIRiS39479.
    RefSeqiNP_001105296.1. NM_001111826.1.
    UniGeneiZm.66285.

    Genome annotation databases

    GeneIDi542214.
    KEGGizma:542214.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65928 mRNA. Translation: CAA46721.1 .
    D14577 mRNA. Translation: BAA03431.1 .
    PIRi S39479.
    RefSeqi NP_001105296.1. NM_001111826.1.
    UniGenei Zm.66285.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2D3A X-ray 2.63 A/B/C/D/E/F/G/H/I/J 1-356 [» ]
    2D3B X-ray 3.50 A/B/C/D/E/F/G/H/I/J 1-356 [» ]
    2D3C X-ray 3.81 A/B/C/D/E/F/G/H/I/J 1-356 [» ]
    ProteinModelPortali P38561.
    SMRi P38561. Positions 3-355.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P38561.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542214.
    KEGGi zma:542214.

    Organism-specific databases

    Gramenei P38561.
    MaizeGDBi 17151.

    Phylogenomic databases

    HOGENOMi HOG000061500.
    KOi K01915.

    Miscellaneous databases

    EvolutionaryTracei P38561.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression of six glutamine synthetase genes in Zea mays."
      Li M.-G., Villemur R., Hussey P.J., Silflow C.D., Gantt J.S., Snustad D.P.
      Plant Mol. Biol. 23:401-407(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. A188.
      Tissue: Seedling.
    2. "Molecular cloning of the family of glutamine synthetase genes from maize: expression of genes for glutamine synthetase and ferredoxin-dependent glutamate synthase in photosynthetic and non-photosynthetic tissues."
      Sakakibara H., Kawabata S., Takahashi H., Hase T., Sugiyama T.
      Plant Cell Physiol. 33:49-58(1992)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Golden cross Bantam T51.
      Tissue: Leaf.

    Entry informationi

    Entry nameiGLNA3_MAIZE
    AccessioniPrimary (citable) accession number: P38561
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3