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Protein

Glutamine synthetase root isozyme 3

Gene

GLN4

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the flow of nitrogen into nitrogenous organic compounds.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 6752.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase root isozyme 3 (EC:6.3.1.2)
Alternative name(s):
GS112
Glutamate--ammonia ligase
Gene namesi
Name:GLN4
Synonyms:GS1-3
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305 Componenti: Unplaced

Organism-specific databases

GrameneiP38561.
MaizeGDBi17151.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Glutamine synthetase root isozyme 3PRO_0000153180Add
BLAST

Proteomic databases

PRIDEiP38561.

Expressioni

Tissue specificityi

Found in all the tissues examined with higher expression found in tissues of the root.

Interactioni

Subunit structurei

Homooctamer.

Structurei

Secondary structure

1
356
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 85Combined sources
Helixi12 – 143Combined sources
Beta strandi19 – 268Combined sources
Beta strandi33 – 4210Combined sources
Helixi47 – 493Combined sources
Beta strandi53 – 564Combined sources
Turni57 – 615Combined sources
Beta strandi65 – 673Combined sources
Beta strandi69 – 7911Combined sources
Turni81 – 833Combined sources
Beta strandi88 – 958Combined sources
Helixi107 – 1159Combined sources
Helixi117 – 1226Combined sources
Beta strandi125 – 13612Combined sources
Turni137 – 1393Combined sources
Beta strandi154 – 1563Combined sources
Turni163 – 1653Combined sources
Helixi169 – 18214Combined sources
Beta strandi186 – 1916Combined sources
Beta strandi197 – 20610Combined sources
Helixi208 – 22821Combined sources
Beta strandi230 – 2334Combined sources
Beta strandi236 – 2394Combined sources
Beta strandi241 – 2433Combined sources
Beta strandi247 – 2537Combined sources
Turni255 – 2573Combined sources
Helixi262 – 27514Combined sources
Helixi277 – 2815Combined sources
Helixi288 – 2914Combined sources
Beta strandi293 – 2975Combined sources
Beta strandi306 – 3094Combined sources
Beta strandi313 – 3175Combined sources
Helixi319 – 3235Combined sources
Beta strandi329 – 3313Combined sources
Helixi340 – 35112Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D3AX-ray2.63A/B/C/D/E/F/G/H/I/J1-356[»]
2D3BX-ray3.50A/B/C/D/E/F/G/H/I/J1-356[»]
2D3CX-ray3.81A/B/C/D/E/F/G/H/I/J1-356[»]
ProteinModelPortaliP38561.
SMRiP38561. Positions 3-355.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38561.

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

HOGENOMiHOG000061500.
KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MACLTDLVNL NLSDTTEKII AEYIWIGGSG MDLRSKARTL SGPVTDPSKL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDCYTPAGE
110 120 130 140 150
PIPTNKRYNA AKIFSSPEVA AEEPWYGIEQ EYTLLQKDTN WPLGWPIGGF
160 170 180 190 200
PGPQGPYYCG IGAEKSFGRD IVDAHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISS GDQVWVARYI LERITEIAGV VVTFDPKPIP GDWNGAGAHT
260 270 280 290 300
NYSTESMRKE GGYEVIKAAI EKLKLRHREH IAAYGEGNDG RLTGRHETAD
310 320 330 340 350
INTFSWGVAN RGASVRVGRE TEQNGKGYFE DRRPASNMDP YVVTSMIAET

TIIWKP
Length:356
Mass (Da):39,169
Last modified:October 1, 1994 - v1
Checksum:i0112DD32D5FB474C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151T → N in BAA03431 (Ref. 2) Curated
Sequence conflicti289 – 2902DG → ER in BAA03431 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65928 mRNA. Translation: CAA46721.1.
D14577 mRNA. Translation: BAA03431.1.
PIRiS39479.
RefSeqiNP_001105296.1. NM_001111826.1.
UniGeneiZm.66285.

Genome annotation databases

GeneIDi542214.
KEGGizma:542214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65928 mRNA. Translation: CAA46721.1.
D14577 mRNA. Translation: BAA03431.1.
PIRiS39479.
RefSeqiNP_001105296.1. NM_001111826.1.
UniGeneiZm.66285.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D3AX-ray2.63A/B/C/D/E/F/G/H/I/J1-356[»]
2D3BX-ray3.50A/B/C/D/E/F/G/H/I/J1-356[»]
2D3CX-ray3.81A/B/C/D/E/F/G/H/I/J1-356[»]
ProteinModelPortaliP38561.
SMRiP38561. Positions 3-355.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP38561.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi542214.
KEGGizma:542214.

Organism-specific databases

GrameneiP38561.
MaizeGDBi17151.

Phylogenomic databases

HOGENOMiHOG000061500.
KOiK01915.

Enzyme and pathway databases

BRENDAi6.3.1.2. 6752.

Miscellaneous databases

EvolutionaryTraceiP38561.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Differential expression of six glutamine synthetase genes in Zea mays."
    Li M.-G., Villemur R., Hussey P.J., Silflow C.D., Gantt J.S., Snustad D.P.
    Plant Mol. Biol. 23:401-407(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. A188.
    Tissue: Seedling.
  2. "Molecular cloning of the family of glutamine synthetase genes from maize: expression of genes for glutamine synthetase and ferredoxin-dependent glutamate synthase in photosynthetic and non-photosynthetic tissues."
    Sakakibara H., Kawabata S., Takahashi H., Hase T., Sugiyama T.
    Plant Cell Physiol. 33:49-58(1992)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Golden cross Bantam T51.
    Tissue: Leaf.

Entry informationi

Entry nameiGLNA3_MAIZE
AccessioniPrimary (citable) accession number: P38561
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 27, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.