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P38561 (GLNA3_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase root isozyme 3

EC=6.3.1.2
Alternative name(s):
GS112
Glutamate--ammonia ligase
Gene names
Name:GLN4
Synonyms:GS1-3
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in the flow of nitrogen into nitrogenous organic compounds.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Tissue specificity

Found in all the tissues examined with higher expression found in tissues of the root.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

leaf senescence

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentcell wall

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytosolic ribosome

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

plasma membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

plasmodesma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

copper ion binding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Glutamine synthetase root isozyme 3
PRO_0000153180

Experimental info

Sequence conflict151T → N in BAA03431. Ref.2
Sequence conflict289 – 2902DG → ER in BAA03431. Ref.2

Secondary structure

..................................................................... 356
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38561 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 0112DD32D5FB474C

FASTA35639,169
        10         20         30         40         50         60 
MACLTDLVNL NLSDTTEKII AEYIWIGGSG MDLRSKARTL SGPVTDPSKL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGEDSEV ILYPQAIFKD PFRRGNNILV MCDCYTPAGE PIPTNKRYNA AKIFSSPEVA 

       130        140        150        160        170        180 
AEEPWYGIEQ EYTLLQKDTN WPLGWPIGGF PGPQGPYYCG IGAEKSFGRD IVDAHYKACL 

       190        200        210        220        230        240 
YAGINISGIN GEVMPGQWEF QVGPSVGISS GDQVWVARYI LERITEIAGV VVTFDPKPIP 

       250        260        270        280        290        300 
GDWNGAGAHT NYSTESMRKE GGYEVIKAAI EKLKLRHREH IAAYGEGNDG RLTGRHETAD 

       310        320        330        340        350 
INTFSWGVAN RGASVRVGRE TEQNGKGYFE DRRPASNMDP YVVTSMIAET TIIWKP 

« Hide

References

[1]"Differential expression of six glutamine synthetase genes in Zea mays."
Li M.-G., Villemur R., Hussey P.J., Silflow C.D., Gantt J.S., Snustad D.P.
Plant Mol. Biol. 23:401-407(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. A188.
Tissue: Seedling.
[2]"Molecular cloning of the family of glutamine synthetase genes from maize: expression of genes for glutamine synthetase and ferredoxin-dependent glutamate synthase in photosynthetic and non-photosynthetic tissues."
Sakakibara H., Kawabata S., Takahashi H., Hase T., Sugiyama T.
Plant Cell Physiol. 33:49-58(1992)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Golden cross Bantam T51.
Tissue: Leaf.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65928 mRNA. Translation: CAA46721.1.
D14577 mRNA. Translation: BAA03431.1.
PIRS39479.
RefSeqNP_001105296.1. NM_001111826.1.
UniGeneZm.66285.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2D3AX-ray2.63A/B/C/D/E/F/G/H/I/J1-356[»]
2D3BX-ray3.50A/B/C/D/E/F/G/H/I/J1-356[»]
2D3CX-ray3.81A/B/C/D/E/F/G/H/I/J1-356[»]
ProteinModelPortalP38561.
SMRP38561. Positions 3-355.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP38561.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID542214.
KEGGzma:542214.

Organism-specific databases

GrameneP38561.
MaizeGDB17151.

Phylogenomic databases

HOGENOMHOG000061500.
KOK01915.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38561.

Entry information

Entry nameGLNA3_MAIZE
AccessionPrimary (citable) accession number: P38561
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references