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P38555 (YPT31_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP-binding protein YPT31/YPT8
Alternative name(s):
Rab GTPase YPT31
Gene names
Name:YPT31
Synonyms:YPT8
Ordered Locus Names:YER031C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for protein transport in the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Plays a role in autophagy. Ref.2 Ref.6 Ref.11 Ref.15

Enzyme regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3. Ref.8 Ref.9

Subunit structure

Interacts with YIF1, YIP1, YIP3, YIP4 and YIP5. Interacts with TRS130. Ref.7 Ref.10 Ref.11 Ref.12

Subcellular location

Golgi apparatus membrane; Lipid-anchor Ref.6.

Miscellaneous

Present with 5955 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 223222GTP-binding protein YPT31/YPT8
PRO_0000121323

Regions

Nucleotide binding20 – 278GTP By similarity
Nucleotide binding68 – 725GTP By similarity
Nucleotide binding126 – 1294GTP By similarity
Motif42 – 509Effector region Probable

Amino acid modifications

Modified residue21N-acetylserine Ref.5
Lipidation2221S-geranylgeranyl cysteine By similarity
Lipidation2231S-geranylgeranyl cysteine By similarity

Secondary structure

............................ 223
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38555 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: EC274E5873138D78

FASTA22324,469
        10         20         30         40         50         60 
MSSEDYGYDY DLLFKIVLIG DSGVGKSNLL SRFTKNEFNM DSKSTIGVEF ATRTLEIDGK 

        70         80         90        100        110        120 
RIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS YENCNHWLSE LRENADDNVA 

       130        140        150        160        170        180 
VGLIGNKSDL AHLRAVPTEE SKTFAQENQL LFTETSALNS ENVDKAFEEL INTIYQKVSK 

       190        200        210        220 
HQMDLGDSSA NGNANGASAP NGPTISLTPT PNENKKANGN NCC 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a gene encoding a new Ypt/Rab-like monomeric G-protein in Saccharomyces cerevisiae."
Lai M.H., Bard M., Kirsch D.R.
Yeast 10:399-402(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast."
Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D.
EMBO J. 15:6460-6475(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]Bienvenut W.V., Peters C.
Submitted (MAY-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-32; 44-61; 77-134 AND 143-177, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment."
Jedd G., Mulholland J., Segev N.
J. Cell Biol. 137:563-580(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p."
Yang X., Matern H.T., Gallwitz D.
EMBO J. 17:4954-4963(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIP1.
Strain: HLR3.
[8]"Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition."
Albert S., Gallwitz D.
J. Biol. Chem. 274:33186-33189(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[9]"The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32."
Jones S., Newman C., Liu F., Segev N.
Mol. Biol. Cell 11:4403-4411(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: ENZYME REGULATION.
[10]"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
Calero M., Collins R.N.
Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIP3.
[11]"Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
Yamamoto K., Jigami Y.
Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TRS130.
[12]"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
Calero M., Winand N.J., Collins R.N.
FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[13]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L17070 Genomic DNA. Translation: AAA83385.1.
X72833 Genomic DNA. Translation: CAA51354.1.
U18778 Genomic DNA. Translation: AAB64564.1.
BK006939 Genomic DNA. Translation: DAA07684.1.
PIRS42679.
RefSeqNP_010948.1. NM_001178922.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3CPJX-ray2.35B1-223[»]
ProteinModelPortalP38555.
SMRP38555. Positions 10-178.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36766. 235 interactions.
DIPDIP-2022N.
IntActP38555. 17 interactions.
MINTMINT-386034.
STRING4932.YER031C.

Proteomic databases

MaxQBP38555.
PaxDbP38555.
PeptideAtlasP38555.
PRIDEP38555.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER031C; YER031C; YER031C.
GeneID856753.
KEGGsce:YER031C.

Organism-specific databases

SGDS000000833. YPT31.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00710000106372.
HOGENOMHOG000233968.
KOK07976.
OMAHDSRATI.
OrthoDBEOG7BGHXR.

Enzyme and pathway databases

BioCycYEAST:G3O-30212-MONOMER.

Gene expression databases

GenevestigatorP38555.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38555.
NextBio982908.

Entry information

Entry nameYPT31_YEAST
AccessionPrimary (citable) accession number: P38555
Secondary accession number(s): D3DLT0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references