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P38555

- YPT31_YEAST

UniProt

P38555 - YPT31_YEAST

Protein

GTP-binding protein YPT31/YPT8

Gene

YPT31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Required for protein transport in the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Plays a role in autophagy.4 Publications

    Enzyme regulationi

    Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 278GTPBy similarity
    Nucleotide bindingi68 – 725GTPBy similarity
    Nucleotide bindingi126 – 1294GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. early endosome to Golgi transport Source: SGD
    3. exocytosis Source: SGD
    4. GTP catabolic process Source: GOC
    5. protein transport Source: UniProtKB-KW
    6. small GTPase mediated signal transduction Source: InterPro
    7. vesicle-mediated transport Source: SGD

    Keywords - Biological processi

    Autophagy, Protein transport, Transport

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30212-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP-binding protein YPT31/YPT8
    Alternative name(s):
    Rab GTPase YPT31
    Gene namesi
    Name:YPT31
    Synonyms:YPT8
    Ordered Locus Names:YER031C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome V

    Organism-specific databases

    SGDiS000000833. YPT31.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Lipid-anchor 1 Publication

    GO - Cellular componenti

    1. endosome Source: SGD
    2. Golgi apparatus Source: SGD
    3. Golgi membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 223222GTP-binding protein YPT31/YPT8PRO_0000121323Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Lipidationi222 – 2221S-geranylgeranyl cysteineBy similarity
    Lipidationi223 – 2231S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Prenylation

    Proteomic databases

    MaxQBiP38555.
    PaxDbiP38555.
    PeptideAtlasiP38555.
    PRIDEiP38555.

    Expressioni

    Gene expression databases

    GenevestigatoriP38555.

    Interactioni

    Subunit structurei

    Interacts with YIF1, YIP1, YIP3, YIP4 and YIP5. Interacts with TRS130.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GDI1P399586EBI-29379,EBI-7517
    YIF1P538453EBI-29379,EBI-28230
    YIP3P536333EBI-29379,EBI-25301
    YIP4P530932EBI-29379,EBI-24124

    Protein-protein interaction databases

    BioGridi36766. 236 interactions.
    DIPiDIP-2022N.
    IntActiP38555. 17 interactions.
    MINTiMINT-386034.
    STRINGi4932.YER031C.

    Structurei

    Secondary structure

    1
    223
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi12 – 209
    Helixi26 – 3510
    Beta strandi49 – 579
    Beta strandi60 – 678
    Turni70 – 767
    Helixi80 – 823
    Turni83 – 853
    Beta strandi88 – 936
    Helixi98 – 11417
    Beta strandi120 – 1256
    Helixi128 – 1336
    Helixi138 – 14710
    Beta strandi151 – 1544
    Helixi163 – 17412

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3CPJX-ray2.35B1-223[»]
    ProteinModelPortaliP38555.
    SMRiP38555. Positions 10-178.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38555.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 509Effector regionCurated

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00710000106372.
    HOGENOMiHOG000233968.
    KOiK07976.
    OMAiHDSRATI.
    OrthoDBiEOG7BGHXR.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38555-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSEDYGYDY DLLFKIVLIG DSGVGKSNLL SRFTKNEFNM DSKSTIGVEF    50
    ATRTLEIDGK RIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS 100
    YENCNHWLSE LRENADDNVA VGLIGNKSDL AHLRAVPTEE SKTFAQENQL 150
    LFTETSALNS ENVDKAFEEL INTIYQKVSK HQMDLGDSSA NGNANGASAP 200
    NGPTISLTPT PNENKKANGN NCC 223
    Length:223
    Mass (Da):24,469
    Last modified:January 23, 2007 - v3
    Checksum:iEC274E5873138D78
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L17070 Genomic DNA. Translation: AAA83385.1.
    X72833 Genomic DNA. Translation: CAA51354.1.
    U18778 Genomic DNA. Translation: AAB64564.1.
    BK006939 Genomic DNA. Translation: DAA07684.1.
    PIRiS42679.
    RefSeqiNP_010948.1. NM_001178922.1.

    Genome annotation databases

    EnsemblFungiiYER031C; YER031C; YER031C.
    GeneIDi856753.
    KEGGisce:YER031C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L17070 Genomic DNA. Translation: AAA83385.1 .
    X72833 Genomic DNA. Translation: CAA51354.1 .
    U18778 Genomic DNA. Translation: AAB64564.1 .
    BK006939 Genomic DNA. Translation: DAA07684.1 .
    PIRi S42679.
    RefSeqi NP_010948.1. NM_001178922.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3CPJ X-ray 2.35 B 1-223 [» ]
    ProteinModelPortali P38555.
    SMRi P38555. Positions 10-178.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36766. 236 interactions.
    DIPi DIP-2022N.
    IntActi P38555. 17 interactions.
    MINTi MINT-386034.
    STRINGi 4932.YER031C.

    Proteomic databases

    MaxQBi P38555.
    PaxDbi P38555.
    PeptideAtlasi P38555.
    PRIDEi P38555.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YER031C ; YER031C ; YER031C .
    GeneIDi 856753.
    KEGGi sce:YER031C.

    Organism-specific databases

    SGDi S000000833. YPT31.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00710000106372.
    HOGENOMi HOG000233968.
    KOi K07976.
    OMAi HDSRATI.
    OrthoDBi EOG7BGHXR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30212-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38555.
    NextBioi 982908.

    Gene expression databases

    Genevestigatori P38555.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a gene encoding a new Ypt/Rab-like monomeric G-protein in Saccharomyces cerevisiae."
      Lai M.H., Bard M., Kirsch D.R.
      Yeast 10:399-402(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast."
      Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D.
      EMBO J. 15:6460-6475(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Bienvenut W.V., Peters C.
      Submitted (MAY-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-32; 44-61; 77-134 AND 143-177, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment."
      Jedd G., Mulholland J., Segev N.
      J. Cell Biol. 137:563-580(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p."
      Yang X., Matern H.T., Gallwitz D.
      EMBO J. 17:4954-4963(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIP1.
      Strain: HLR3.
    8. "Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition."
      Albert S., Gallwitz D.
      J. Biol. Chem. 274:33186-33189(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    9. "The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32."
      Jones S., Newman C., Liu F., Segev N.
      Mol. Biol. Cell 11:4403-4411(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
    10. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
      Calero M., Collins R.N.
      Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIP3.
    11. "Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
      Yamamoto K., Jigami Y.
      Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH TRS130.
    12. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
      Calero M., Winand N.J., Collins R.N.
      FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
    13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
      Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
      Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiYPT31_YEAST
    AccessioniPrimary (citable) accession number: P38555
    Secondary accession number(s): D3DLT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 142 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5955 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome V
      Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

    External Data

    Dasty 3