Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P38555

- YPT31_YEAST

UniProt

P38555 - YPT31_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

GTP-binding protein YPT31/YPT8

Gene

YPT31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for protein transport in the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Plays a role in autophagy.4 Publications

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 278GTPBy similarity
Nucleotide bindingi68 – 725GTPBy similarity
Nucleotide bindingi126 – 1294GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. early endosome to Golgi transport Source: SGD
  3. exocytosis Source: SGD
  4. GTP catabolic process Source: GOC
  5. protein transport Source: UniProtKB-KW
  6. small GTPase mediated signal transduction Source: InterPro
  7. vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30212-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT31/YPT8
Alternative name(s):
Rab GTPase YPT31
Gene namesi
Name:YPT31
Synonyms:YPT8
Ordered Locus Names:YER031C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000833. YPT31.

Subcellular locationi

Golgi apparatus membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. endosome Source: SGD
  2. Golgi apparatus Source: SGD
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 223222GTP-binding protein YPT31/YPT8PRO_0000121323Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Lipidationi222 – 2221S-geranylgeranyl cysteineBy similarity
Lipidationi223 – 2231S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

MaxQBiP38555.
PaxDbiP38555.
PeptideAtlasiP38555.
PRIDEiP38555.

Expressioni

Gene expression databases

GenevestigatoriP38555.

Interactioni

Subunit structurei

Interacts with YIF1, YIP1, YIP3, YIP4 and YIP5. Interacts with TRS130.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GDI1P399586EBI-29379,EBI-7517
YIF1P538453EBI-29379,EBI-28230
YIP3P536333EBI-29379,EBI-25301
YIP4P530932EBI-29379,EBI-24124

Protein-protein interaction databases

BioGridi36766. 237 interactions.
DIPiDIP-2022N.
IntActiP38555. 17 interactions.
MINTiMINT-386034.
STRINGi4932.YER031C.

Structurei

Secondary structure

1
223
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 209Combined sources
Helixi26 – 3510Combined sources
Beta strandi49 – 579Combined sources
Beta strandi60 – 678Combined sources
Turni70 – 767Combined sources
Helixi80 – 823Combined sources
Turni83 – 853Combined sources
Beta strandi88 – 936Combined sources
Helixi98 – 11417Combined sources
Beta strandi120 – 1256Combined sources
Helixi128 – 1336Combined sources
Helixi138 – 14710Combined sources
Beta strandi151 – 1544Combined sources
Helixi163 – 17412Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CPJX-ray2.35B1-223[»]
ProteinModelPortaliP38555.
SMRiP38555. Positions 10-178.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38555.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 509Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiP38555.
KOiK07976.
OMAiHDSRATI.
OrthoDBiEOG7BGHXR.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38555-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSEDYGYDY DLLFKIVLIG DSGVGKSNLL SRFTKNEFNM DSKSTIGVEF
60 70 80 90 100
ATRTLEIDGK RIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS
110 120 130 140 150
YENCNHWLSE LRENADDNVA VGLIGNKSDL AHLRAVPTEE SKTFAQENQL
160 170 180 190 200
LFTETSALNS ENVDKAFEEL INTIYQKVSK HQMDLGDSSA NGNANGASAP
210 220
NGPTISLTPT PNENKKANGN NCC
Length:223
Mass (Da):24,469
Last modified:January 23, 2007 - v3
Checksum:iEC274E5873138D78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17070 Genomic DNA. Translation: AAA83385.1.
X72833 Genomic DNA. Translation: CAA51354.1.
U18778 Genomic DNA. Translation: AAB64564.1.
BK006939 Genomic DNA. Translation: DAA07684.1.
PIRiS42679.
RefSeqiNP_010948.1. NM_001178922.1.

Genome annotation databases

EnsemblFungiiYER031C; YER031C; YER031C.
GeneIDi856753.
KEGGisce:YER031C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17070 Genomic DNA. Translation: AAA83385.1 .
X72833 Genomic DNA. Translation: CAA51354.1 .
U18778 Genomic DNA. Translation: AAB64564.1 .
BK006939 Genomic DNA. Translation: DAA07684.1 .
PIRi S42679.
RefSeqi NP_010948.1. NM_001178922.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3CPJ X-ray 2.35 B 1-223 [» ]
ProteinModelPortali P38555.
SMRi P38555. Positions 10-178.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36766. 237 interactions.
DIPi DIP-2022N.
IntActi P38555. 17 interactions.
MINTi MINT-386034.
STRINGi 4932.YER031C.

Proteomic databases

MaxQBi P38555.
PaxDbi P38555.
PeptideAtlasi P38555.
PRIDEi P38555.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER031C ; YER031C ; YER031C .
GeneIDi 856753.
KEGGi sce:YER031C.

Organism-specific databases

SGDi S000000833. YPT31.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000118841.
HOGENOMi HOG000233968.
InParanoidi P38555.
KOi K07976.
OMAi HDSRATI.
OrthoDBi EOG7BGHXR.

Enzyme and pathway databases

BioCyci YEAST:G3O-30212-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38555.
NextBioi 982908.

Gene expression databases

Genevestigatori P38555.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a gene encoding a new Ypt/Rab-like monomeric G-protein in Saccharomyces cerevisiae."
    Lai M.H., Bard M., Kirsch D.R.
    Yeast 10:399-402(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast."
    Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D.
    EMBO J. 15:6460-6475(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Bienvenut W.V., Peters C.
    Submitted (MAY-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-32; 44-61; 77-134 AND 143-177, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment."
    Jedd G., Mulholland J., Segev N.
    J. Cell Biol. 137:563-580(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p."
    Yang X., Matern H.T., Gallwitz D.
    EMBO J. 17:4954-4963(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIP1.
    Strain: HLR3.
  8. "Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition."
    Albert S., Gallwitz D.
    J. Biol. Chem. 274:33186-33189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  9. "The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32."
    Jones S., Newman C., Liu F., Segev N.
    Mol. Biol. Cell 11:4403-4411(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: ENZYME REGULATION.
  10. "Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins."
    Calero M., Collins R.N.
    Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIP3.
  11. "Mutation of TRS130, which encodes a component of the TRAPP II complex, activates transcription of OCH1 in Saccharomyces cerevisiae."
    Yamamoto K., Jigami Y.
    Curr. Genet. 42:85-93(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TRS130.
  12. "Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors."
    Calero M., Winand N.J., Collins R.N.
    FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae."
    Zou S., Chen Y., Liu Y., Segev N., Yu S., Liu Y., Min G., Ye M., Zeng Y., Zhu X., Hong B., Bjorn L.O., Liang Y., Li S., Xie Z.
    Traffic 14:233-246(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiYPT31_YEAST
AccessioniPrimary (citable) accession number: P38555
Secondary accession number(s): D3DLT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5955 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3