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Protein

GTP-binding protein YPT31/YPT8

Gene

YPT31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for protein transport in the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Plays a role in autophagy.4 Publications

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi20 – 27GTPBy similarity8
Nucleotide bindingi68 – 72GTPBy similarity5
Nucleotide bindingi126 – 129GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • early endosome to Golgi transport Source: SGD
  • exocytosis Source: SGD
  • protein transport Source: UniProtKB-KW
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30212-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein YPT31/YPT8
Alternative name(s):
Rab GTPase YPT31
Gene namesi
Name:YPT31
Synonyms:YPT8
Ordered Locus Names:YER031C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER031C.
SGDiS000000833. YPT31.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GOC
  • endosome Source: SGD
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: UniProtKB-SubCell
  • recycling endosome Source: GO_Central
  • trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001213232 – 223GTP-binding protein YPT31/YPT8Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Lipidationi222S-geranylgeranyl cysteineBy similarity1
Lipidationi223S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Prenylation

Proteomic databases

MaxQBiP38555.
PRIDEiP38555.

PTM databases

iPTMnetiP38555.

Interactioni

Subunit structurei

Interacts with YIF1, YIP1, YIP3, YIP4 and YIP5. Interacts with TRS130.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GDI1P399586EBI-29379,EBI-7517
YIF1P538453EBI-29379,EBI-28230
YIP3P536333EBI-29379,EBI-25301
YIP4P530932EBI-29379,EBI-24124

Protein-protein interaction databases

BioGridi36766. 250 interactors.
DIPiDIP-2022N.
IntActiP38555. 17 interactors.
MINTiMINT-386034.

Structurei

Secondary structure

1223
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 20Combined sources9
Helixi26 – 35Combined sources10
Beta strandi49 – 57Combined sources9
Beta strandi60 – 67Combined sources8
Turni70 – 76Combined sources7
Helixi80 – 82Combined sources3
Turni83 – 85Combined sources3
Beta strandi88 – 93Combined sources6
Helixi98 – 114Combined sources17
Beta strandi120 – 125Combined sources6
Helixi128 – 133Combined sources6
Helixi138 – 147Combined sources10
Beta strandi151 – 154Combined sources4
Helixi163 – 174Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CPJX-ray2.35B1-223[»]
ProteinModelPortaliP38555.
SMRiP38555.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38555.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 50Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiP38555.
KOiK07905.
OMAiQFASENQ.
OrthoDBiEOG092C5MDH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38555-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEDYGYDY DLLFKIVLIG DSGVGKSNLL SRFTKNEFNM DSKSTIGVEF
60 70 80 90 100
ATRTLEIDGK RIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS
110 120 130 140 150
YENCNHWLSE LRENADDNVA VGLIGNKSDL AHLRAVPTEE SKTFAQENQL
160 170 180 190 200
LFTETSALNS ENVDKAFEEL INTIYQKVSK HQMDLGDSSA NGNANGASAP
210 220
NGPTISLTPT PNENKKANGN NCC
Length:223
Mass (Da):24,469
Last modified:January 23, 2007 - v3
Checksum:iEC274E5873138D78
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17070 Genomic DNA. Translation: AAA83385.1.
X72833 Genomic DNA. Translation: CAA51354.1.
U18778 Genomic DNA. Translation: AAB64564.1.
BK006939 Genomic DNA. Translation: DAA07684.1.
PIRiS42679.
RefSeqiNP_010948.1. NM_001178922.1.

Genome annotation databases

EnsemblFungiiYER031C; YER031C; YER031C.
GeneIDi856753.
KEGGisce:YER031C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L17070 Genomic DNA. Translation: AAA83385.1.
X72833 Genomic DNA. Translation: CAA51354.1.
U18778 Genomic DNA. Translation: AAB64564.1.
BK006939 Genomic DNA. Translation: DAA07684.1.
PIRiS42679.
RefSeqiNP_010948.1. NM_001178922.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CPJX-ray2.35B1-223[»]
ProteinModelPortaliP38555.
SMRiP38555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36766. 250 interactors.
DIPiDIP-2022N.
IntActiP38555. 17 interactors.
MINTiMINT-386034.

PTM databases

iPTMnetiP38555.

Proteomic databases

MaxQBiP38555.
PRIDEiP38555.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYER031C; YER031C; YER031C.
GeneIDi856753.
KEGGisce:YER031C.

Organism-specific databases

EuPathDBiFungiDB:YER031C.
SGDiS000000833. YPT31.

Phylogenomic databases

GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiP38555.
KOiK07905.
OMAiQFASENQ.
OrthoDBiEOG092C5MDH.

Enzyme and pathway databases

BioCyciYEAST:G3O-30212-MONOMER.
ReactomeiR-SCE-1445148. Translocation of GLUT4 to the plasma membrane.
R-SCE-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiP38555.
PROiP38555.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYPT31_YEAST
AccessioniPrimary (citable) accession number: P38555
Secondary accession number(s): D3DLT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5955 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.