GTP-binding protein YPT31/YPT8 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38555 (YPT31_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 130. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
GTP-binding protein YPT31/YPT8

Alternative name(s):
Rab GTPase YPT31

Gene names

Name:	YPT31
Synonyms:	YPT8
Ordered Locus Names:	YER031C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for protein transport in the secretory pathway. Probably involved in regulation of secretory vesicle formation at the trans-Golgi compartment. Ref.2 Ref.6
Enzyme regulation	Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) TRAPP complex, and inactivated by GTPase-activating protein (GAP) GYP3. Ref.8 Ref.9
Subunit structure	Interacts with YIF1, YIP1, YIP3, YIP4 and YIP5. Ref.7 Ref.10 Ref.11
Subcellular location	Golgi apparatus membrane; Lipid-anchor Ref.6.
Miscellaneous	Present with 5955 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	early endosome to Golgi transport Inferred from genetic interaction PubMed 15537705. Source: SGD exocytosis Inferred from mutant phenotype Ref.6. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 15537705. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from direct assay PubMed 15537705. Source: SGD mitochondrial outer membrane Inferred from direct assay PubMed 16407407. Source: SGD
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay PubMed 11210571. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
GDI1	P39958	8	EBI-29379,EBI-7517
YIF1	P53845	3	EBI-29379,EBI-28230
YIP3	P53633	3	EBI-29379,EBI-25301
YIP4	P53093	2	EBI-29379,EBI-24124

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5

Chain

2 – 223

222

GTP-binding protein YPT31/YPT8

PRO_0000121323

Regions

Nucleotide binding

20 – 27

GTP By similarity

Nucleotide binding

68 – 72

GTP By similarity

Nucleotide binding

126 – 129

GTP By similarity

Motif

42 – 50

Effector region Probable

Amino acid modifications

Modified residue

N-acetylserine Ref.5

Modified residue

Phosphoserine Ref.13

Modified residue

Phosphothreonine Ref.13

Lipidation

222

S-geranylgeranyl cysteine By similarity

Lipidation

223

S-geranylgeranyl cysteine By similarity

Secondary structure

223

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P38555 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: EC274E5873138D78
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FASTA

223

24,469

        10         20         30         40         50         60 
MSSEDYGYDY DLLFKIVLIG DSGVGKSNLL SRFTKNEFNM DSKSTIGVEF ATRTLEIDGK 

        70         80         90        100        110        120 
RIKAQIWDTA GQERYRAITS AYYRGAVGAL IVYDISKSSS YENCNHWLSE LRENADDNVA 

       130        140        150        160        170        180 
VGLIGNKSDL AHLRAVPTEE SKTFAQENQL LFTETSALNS ENVDKAFEEL INTIYQKVSK 

       190        200        210        220 
HQMDLGDSSA NGNANGASAP NGPTISLTPT PNENKKANGN NCC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a gene encoding a new Ypt/Rab-like monomeric G-protein in Saccharomyces cerevisiae." Lai M.H., Bard M., Kirsch D.R. Yeast 10:399-402(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast." Benli M., Doering F., Robinson D.G., Yang X., Gallwitz D. EMBO J. 15:6460-6475(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	Bienvenut W.V., Peters C. Submitted (MAY-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-32; 44-61; 77-134 AND 143-177, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT SER-2, MASS SPECTROMETRY.
[6]	"Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment." Jedd G., Mulholland J., Segev N. J. Cell Biol. 137:563-580(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p." Yang X., Matern H.T., Gallwitz D. EMBO J. 17:4954-4963(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH YIP1. Strain: HLR3.
[8]	"Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition." Albert S., Gallwitz D. J. Biol. Chem. 274:33186-33189(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION.
[9]	"The TRAPP complex is a nucleotide exchanger for Ypt1 and Ypt31/32." Jones S., Newman C., Liu F., Segev N. Mol. Biol. Cell 11:4403-4411(2000) [PubMed] [Europe PMC] [Abstract] Cited for: ENZYME REGULATION.
[10]	"Saccharomyces cerevisiae Pra1p/Yip3p interacts with Yip1p and Rab proteins." Calero M., Collins R.N. Biochem. Biophys. Res. Commun. 290:676-681(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH YIP3.
[11]	"Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors." Calero M., Winand N.J., Collins R.N. FEBS Lett. 515:89-98(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH YIF1; YIP4 AND YIP5.
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND THR-45, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L17070 Genomic DNA. Translation: AAA83385.1.
X72833 Genomic DNA. Translation: CAA51354.1.
U18778 Genomic DNA. Translation: AAB64564.1.
BK006939 Genomic DNA. Translation: DAA07684.1.

PIR

S42679.

RefSeq

NP_010948.1. NM_001178922.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CPJ	X-ray	2.35	B	1-223	[»]

ProteinModelPortal

P38555.

SMR

P38555. Positions 10-178.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2022N.

IntAct

P38555. 16 interactions.

MINT

MINT-386034.

STRING

4932.YER031C.

Proteomic databases

PaxDb

P38555.

PeptideAtlas

P38555.

PRIDE

P38555.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YER031C; YER031C; YER031C.

GeneID

856753.

KEGG

sce:YER031C.

Organism-specific databases

SGD

S000000833. YPT31.

Phylogenomic databases

eggNOG

COG1100.

GeneTree

ENSGT00690000101664.

HOGENOM

HOG000233968.

K07976.

OMA

ERSAHDE.

OrthoDB

EOG41K2ND.

Gene expression databases

Genevestigator

P38555.

GermOnline

YER031C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P38555.

NextBio

982908.

Entry information

Entry name

YPT31_YEAST

Accession

Primary (citable) accession number: P38555
Secondary accession number(s): D3DLT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 130 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents