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P38533

- HSF2_MOUSE

UniProt

P38533 - HSF2_MOUSE

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Protein
Heat shock factor protein 2
Gene
Hsf2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi7 – 112106 By similarity
Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Source: Ensembl
  3. protein homodimerization activity Source: MGI
  4. sequence-specific DNA binding Source: MGI
  5. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
  2. response to stress Source: UniProtKB-KW
  3. spermatogenesis Source: MGI
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein 2
Short name:
HSF 2
Alternative name(s):
Heat shock transcription factor 2
Short name:
HSTF 2
Gene namesi
Name:Hsf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:96239. Hsf2.

Subcellular locationi

Cytoplasm. Nucleus
Note: Cytoplasmic during normal growth and moves to the nucleus upon activation.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Heat shock factor protein 2
PRO_0000124570Add
BLAST

Proteomic databases

PRIDEiP38533.

PTM databases

PhosphoSiteiP38533.

Expressioni

Tissue specificityi

Isoform alpha is expressed predominantly in testis while isoform beta is expressed predominantly in heart and brain.1 Publication

Developmental stagei

In the 7-day-old testis, isoform beta is expressed at significantly higher levels than isoform alpha. As development proceeds, the levels of isoform alpha gradually increase so that it is the predominant isoform in mature testes.1 Publication

Gene expression databases

BgeeiP38533.
CleanExiMM_HSF2.
GenevestigatoriP38533.

Interactioni

Subunit structurei

DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP38533.
SMRiP38533. Positions 1-113.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 19274Hydrophobic repeat HR-A/B
Add
BLAST
Regioni359 – 38426Hydrophobic repeat HR-C
Add
BLAST

Sequence similaritiesi

Belongs to the HSF family.

Phylogenomic databases

eggNOGiCOG5169.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiP38533.
KOiK09415.
OMAiPKTDKQL.
OrthoDBiEOG7353WM.
PhylomeDBiP38533.
TreeFamiTF330401.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027712. HSF2.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF144. PTHR10015:SF144. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Alpha (identifier: P38533-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP    50
KYFKHNNMAS FVRQLNMYGF RKVVHIESGI IKQERDGPVE FQHPYFKQGQ 100
DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ ETIESRLSEL 150
KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL 200
LLNTNGAPKK NLYQHIVKEP TDNHHHKVPH SRTEGLKSRE RISDDIIIYD 250
VTDDNVDEEN IPVIPETNED VVVDSSNQYP DIVIVEDDNE DEYAPVIQSG 300
EQSEPAREPL RVGSAGSSSP LMSSAVQLNG SSSLTSEDPV TMMDSILNDN 350
INLLGKVELL DYLDSIDCSL EDFQAMLSGR QFSIDPDLLV DLFTSSVQMN 400
PTDNIKYTKS ENKGLEATKS SVVQHVSEEG RKSKSKPDKQ LIQYTAFPLL 450
AFLDGNSASA IEQGSTTASS EVVPSVDKPI EVDELLDSSL DPEPTQSKLV 500
RLEPLTEAEA SEATLFYLCE LAPAPLDSDM PLLDS 535
Length:535
Mass (Da):60,225
Last modified:August 30, 2002 - v2
Checksum:i47972520402ABB13
GO
Isoform Beta (identifier: P38533-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     392-409: Missing.

Show »
Length:517
Mass (Da):58,155
Checksum:i098965BBCDF4E1B0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei392 – 40918Missing in isoform Beta.
VSP_002417Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61754 mRNA. Translation: CAA43893.1.
AF045627
, AF045615, AF045616, AF045617, AF045618, AF045619, AF045620, AF045621, AF045622, AF045623, AF045624, AF045625, AF045626 Genomic DNA. Translation: AAD02417.1.
S79629 mRNA. Translation: AAB35307.1.
BC018414 mRNA. Translation: AAH18414.1.
CCDSiCCDS23852.1. [P38533-2]
PIRiB40583.
RefSeqiNP_032323.3. NM_008297.3. [P38533-2]
UniGeneiMm.261395.
Mm.447362.

Genome annotation databases

EnsembliENSMUST00000079833; ENSMUSP00000078761; ENSMUSG00000019878. [P38533-2]
GeneIDi15500.
KEGGimmu:15500.
UCSCiuc007fck.2. mouse. [P38533-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X61754 mRNA. Translation: CAA43893.1 .
AF045627
, AF045615 , AF045616 , AF045617 , AF045618 , AF045619 , AF045620 , AF045621 , AF045622 , AF045623 , AF045624 , AF045625 , AF045626 Genomic DNA. Translation: AAD02417.1 .
S79629 mRNA. Translation: AAB35307.1 .
BC018414 mRNA. Translation: AAH18414.1 .
CCDSi CCDS23852.1. [P38533-2 ]
PIRi B40583.
RefSeqi NP_032323.3. NM_008297.3. [P38533-2 ]
UniGenei Mm.261395.
Mm.447362.

3D structure databases

ProteinModelPortali P38533.
SMRi P38533. Positions 1-113.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P38533.

Proteomic databases

PRIDEi P38533.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000079833 ; ENSMUSP00000078761 ; ENSMUSG00000019878 . [P38533-2 ]
GeneIDi 15500.
KEGGi mmu:15500.
UCSCi uc007fck.2. mouse. [P38533-2 ]

Organism-specific databases

CTDi 3298.
MGIi MGI:96239. Hsf2.

Phylogenomic databases

eggNOGi COG5169.
GeneTreei ENSGT00390000001182.
HOGENOMi HOG000253917.
HOVERGENi HBG005999.
InParanoidi P38533.
KOi K09415.
OMAi PKTDKQL.
OrthoDBi EOG7353WM.
PhylomeDBi P38533.
TreeFami TF330401.

Miscellaneous databases

NextBioi 288394.
PROi P38533.
SOURCEi Search...

Gene expression databases

Bgeei P38533.
CleanExi MM_HSF2.
Genevestigatori P38533.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR027712. HSF2.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR10015. PTHR10015. 1 hit.
PTHR10015:SF144. PTHR10015:SF144. 1 hit.
Pfami PF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view ]
PRINTSi PR00056. HSFDOMAIN.
SMARTi SM00415. HSF. 1 hit.
[Graphical view ]
PROSITEi PS00434. HSF_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability."
    Sarge K.D., Zimarino V., Holm K., Wu C., Morimoto R.I.
    Genes Dev. 5:1902-1911(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
    Strain: BALB/c.
    Tissue: Liver.
  2. "Genomic structure and chromosomal localization of the mouse Hsf2 gene and promoter sequences."
    Manuel M., Sage J., Mattei M.-G., Morange M., Mezger V.
    Gene 232:115-124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM ALPHA).
    Strain: 129.
  3. "Tissue-dependent expression of heat shock factor 2 isoforms with distinct transcriptional activities."
    Goodson M.L., Park-Sarge O.-K., Sarge K.D.
    Mol. Cell. Biol. 15:5288-5293(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: CBA/J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).

Entry informationi

Entry nameiHSF2_MOUSE
AccessioniPrimary (citable) accession number: P38533
Secondary accession number(s): Q64157, Q8VEJ0, Q9Z2L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 30, 2002
Last modified: July 9, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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