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Protein

Heat shock factor protein 2

Gene

Hsf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi7 – 112106By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein 2
Short name:
HSF 2
Alternative name(s):
Heat shock transcription factor 2
Short name:
HSTF 2
Gene namesi
Name:Hsf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:96239. Hsf2.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Cytoplasmic during normal growth and moves to the nucleus upon activation.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Heat shock factor protein 2PRO_0000124570Add
BLAST

Proteomic databases

MaxQBiP38533.
PRIDEiP38533.

PTM databases

PhosphoSiteiP38533.

Expressioni

Tissue specificityi

Isoform alpha is expressed predominantly in testis while isoform beta is expressed predominantly in heart and brain.1 Publication

Developmental stagei

In the 7-day-old testis, isoform beta is expressed at significantly higher levels than isoform alpha. As development proceeds, the levels of isoform alpha gradually increase so that it is the predominant isoform in mature testes.1 Publication

Gene expression databases

BgeeiP38533.
CleanExiMM_HSF2.
GenevisibleiP38533. MM.

Interactioni

Subunit structurei

DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078761.

Structurei

3D structure databases

ProteinModelPortaliP38533.
SMRiP38533. Positions 1-113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 19274Hydrophobic repeat HR-A/BAdd
BLAST
Regioni359 – 38426Hydrophobic repeat HR-CAdd
BLAST

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

eggNOGiCOG5169.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiP38533.
KOiK09415.
OMAiISDPSNC.
OrthoDBiEOG7353WM.
PhylomeDBiP38533.
TreeFamiTF330401.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027712. HSF2.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF144. PTHR10015:SF144. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: P38533-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP
60 70 80 90 100
KYFKHNNMAS FVRQLNMYGF RKVVHIESGI IKQERDGPVE FQHPYFKQGQ
110 120 130 140 150
DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ ETIESRLSEL
160 170 180 190 200
KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL
210 220 230 240 250
LLNTNGAPKK NLYQHIVKEP TDNHHHKVPH SRTEGLKSRE RISDDIIIYD
260 270 280 290 300
VTDDNVDEEN IPVIPETNED VVVDSSNQYP DIVIVEDDNE DEYAPVIQSG
310 320 330 340 350
EQSEPAREPL RVGSAGSSSP LMSSAVQLNG SSSLTSEDPV TMMDSILNDN
360 370 380 390 400
INLLGKVELL DYLDSIDCSL EDFQAMLSGR QFSIDPDLLV DLFTSSVQMN
410 420 430 440 450
PTDNIKYTKS ENKGLEATKS SVVQHVSEEG RKSKSKPDKQ LIQYTAFPLL
460 470 480 490 500
AFLDGNSASA IEQGSTTASS EVVPSVDKPI EVDELLDSSL DPEPTQSKLV
510 520 530
RLEPLTEAEA SEATLFYLCE LAPAPLDSDM PLLDS
Length:535
Mass (Da):60,225
Last modified:August 30, 2002 - v2
Checksum:i47972520402ABB13
GO
Isoform Beta (identifier: P38533-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     392-409: Missing.

Show »
Length:517
Mass (Da):58,155
Checksum:i098965BBCDF4E1B0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei392 – 40918Missing in isoform Beta. 3 PublicationsVSP_002417Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61754 mRNA. Translation: CAA43893.1.
AF045627
, AF045615, AF045616, AF045617, AF045618, AF045619, AF045620, AF045621, AF045622, AF045623, AF045624, AF045625, AF045626 Genomic DNA. Translation: AAD02417.1.
S79629 mRNA. Translation: AAB35307.1.
BC018414 mRNA. Translation: AAH18414.1.
CCDSiCCDS23852.1. [P38533-2]
PIRiB40583.
RefSeqiNP_032323.3. NM_008297.3. [P38533-2]
UniGeneiMm.261395.
Mm.447362.

Genome annotation databases

EnsembliENSMUST00000079833; ENSMUSP00000078761; ENSMUSG00000019878. [P38533-2]
GeneIDi15500.
KEGGimmu:15500.
UCSCiuc007fck.2. mouse. [P38533-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61754 mRNA. Translation: CAA43893.1.
AF045627
, AF045615, AF045616, AF045617, AF045618, AF045619, AF045620, AF045621, AF045622, AF045623, AF045624, AF045625, AF045626 Genomic DNA. Translation: AAD02417.1.
S79629 mRNA. Translation: AAB35307.1.
BC018414 mRNA. Translation: AAH18414.1.
CCDSiCCDS23852.1. [P38533-2]
PIRiB40583.
RefSeqiNP_032323.3. NM_008297.3. [P38533-2]
UniGeneiMm.261395.
Mm.447362.

3D structure databases

ProteinModelPortaliP38533.
SMRiP38533. Positions 1-113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078761.

PTM databases

PhosphoSiteiP38533.

Proteomic databases

MaxQBiP38533.
PRIDEiP38533.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079833; ENSMUSP00000078761; ENSMUSG00000019878. [P38533-2]
GeneIDi15500.
KEGGimmu:15500.
UCSCiuc007fck.2. mouse. [P38533-2]

Organism-specific databases

CTDi3298.
MGIiMGI:96239. Hsf2.

Phylogenomic databases

eggNOGiCOG5169.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiP38533.
KOiK09415.
OMAiISDPSNC.
OrthoDBiEOG7353WM.
PhylomeDBiP38533.
TreeFamiTF330401.

Miscellaneous databases

NextBioi288394.
PROiP38533.
SOURCEiSearch...

Gene expression databases

BgeeiP38533.
CleanExiMM_HSF2.
GenevisibleiP38533. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027712. HSF2.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PTHR10015:SF144. PTHR10015:SF144. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability."
    Sarge K.D., Zimarino V., Holm K., Wu C., Morimoto R.I.
    Genes Dev. 5:1902-1911(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
    Strain: BALB/c.
    Tissue: Liver.
  2. "Genomic structure and chromosomal localization of the mouse Hsf2 gene and promoter sequences."
    Manuel M., Sage J., Mattei M.-G., Morange M., Mezger V.
    Gene 232:115-124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM ALPHA).
    Strain: 129.
  3. "Tissue-dependent expression of heat shock factor 2 isoforms with distinct transcriptional activities."
    Goodson M.L., Park-Sarge O.-K., Sarge K.D.
    Mol. Cell. Biol. 15:5288-5293(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: CBA/J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).

Entry informationi

Entry nameiHSF2_MOUSE
AccessioniPrimary (citable) accession number: P38533
Secondary accession number(s): Q64157, Q8VEJ0, Q9Z2L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 30, 2002
Last modified: July 22, 2015
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.