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P38533

- HSF2_MOUSE

UniProt

P38533 - HSF2_MOUSE

Protein

Heat shock factor protein 2

Gene

Hsf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (30 Aug 2002)
      Previous versions | rss
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    Functioni

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi7 – 112106By similarityAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein homodimerization activity Source: MGI
    3. RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Source: Ensembl
    4. sequence-specific DNA binding Source: MGI
    5. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    2. response to stress Source: UniProtKB-KW
    3. spermatogenesis Source: MGI
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Stress response, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat shock factor protein 2
    Short name:
    HSF 2
    Alternative name(s):
    Heat shock transcription factor 2
    Short name:
    HSTF 2
    Gene namesi
    Name:Hsf2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:96239. Hsf2.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Cytoplasmic during normal growth and moves to the nucleus upon activation.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 535535Heat shock factor protein 2PRO_0000124570Add
    BLAST

    Proteomic databases

    PRIDEiP38533.

    PTM databases

    PhosphoSiteiP38533.

    Expressioni

    Tissue specificityi

    Isoform alpha is expressed predominantly in testis while isoform beta is expressed predominantly in heart and brain.1 Publication

    Developmental stagei

    In the 7-day-old testis, isoform beta is expressed at significantly higher levels than isoform alpha. As development proceeds, the levels of isoform alpha gradually increase so that it is the predominant isoform in mature testes.1 Publication

    Gene expression databases

    BgeeiP38533.
    CleanExiMM_HSF2.
    GenevestigatoriP38533.

    Interactioni

    Subunit structurei

    DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP38533.
    SMRiP38533. Positions 1-113.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni119 – 19274Hydrophobic repeat HR-A/BAdd
    BLAST
    Regioni359 – 38426Hydrophobic repeat HR-CAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HSF family.Curated

    Phylogenomic databases

    eggNOGiCOG5169.
    GeneTreeiENSGT00390000001182.
    HOGENOMiHOG000253917.
    HOVERGENiHBG005999.
    InParanoidiP38533.
    KOiK09415.
    OMAiPKTDKQL.
    OrthoDBiEOG7353WM.
    PhylomeDBiP38533.
    TreeFamiTF330401.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR027712. HSF2.
    IPR000232. HSF_DNA-bd.
    IPR027725. HSF_fam.
    IPR010542. Vert_HSTF_C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10015. PTHR10015. 1 hit.
    PTHR10015:SF144. PTHR10015:SF144. 1 hit.
    PfamiPF00447. HSF_DNA-bind. 1 hit.
    PF06546. Vert_HS_TF. 1 hit.
    [Graphical view]
    PRINTSiPR00056. HSFDOMAIN.
    SMARTiSM00415. HSF. 1 hit.
    [Graphical view]
    PROSITEiPS00434. HSF_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Alpha (identifier: P38533-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKQSSNVPAF LSKLWTLVEE THTNEFITWS QNGQSFLVLD EQRFAKEILP    50
    KYFKHNNMAS FVRQLNMYGF RKVVHIESGI IKQERDGPVE FQHPYFKQGQ 100
    DDLLENIKRK VSSSKPEENK IRQEDLTKII SSAQKVQIKQ ETIESRLSEL 150
    KSENESLWKE VSELRAKHAQ QQQVIRKIVQ FIVTLVQNNQ LVSLKRKRPL 200
    LLNTNGAPKK NLYQHIVKEP TDNHHHKVPH SRTEGLKSRE RISDDIIIYD 250
    VTDDNVDEEN IPVIPETNED VVVDSSNQYP DIVIVEDDNE DEYAPVIQSG 300
    EQSEPAREPL RVGSAGSSSP LMSSAVQLNG SSSLTSEDPV TMMDSILNDN 350
    INLLGKVELL DYLDSIDCSL EDFQAMLSGR QFSIDPDLLV DLFTSSVQMN 400
    PTDNIKYTKS ENKGLEATKS SVVQHVSEEG RKSKSKPDKQ LIQYTAFPLL 450
    AFLDGNSASA IEQGSTTASS EVVPSVDKPI EVDELLDSSL DPEPTQSKLV 500
    RLEPLTEAEA SEATLFYLCE LAPAPLDSDM PLLDS 535
    Length:535
    Mass (Da):60,225
    Last modified:August 30, 2002 - v2
    Checksum:i47972520402ABB13
    GO
    Isoform Beta (identifier: P38533-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         392-409: Missing.

    Show »
    Length:517
    Mass (Da):58,155
    Checksum:i098965BBCDF4E1B0
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei392 – 40918Missing in isoform Beta. 3 PublicationsVSP_002417Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61754 mRNA. Translation: CAA43893.1.
    AF045627
    , AF045615, AF045616, AF045617, AF045618, AF045619, AF045620, AF045621, AF045622, AF045623, AF045624, AF045625, AF045626 Genomic DNA. Translation: AAD02417.1.
    S79629 mRNA. Translation: AAB35307.1.
    BC018414 mRNA. Translation: AAH18414.1.
    CCDSiCCDS23852.1. [P38533-2]
    PIRiB40583.
    RefSeqiNP_032323.3. NM_008297.3. [P38533-2]
    UniGeneiMm.261395.
    Mm.447362.

    Genome annotation databases

    EnsembliENSMUST00000079833; ENSMUSP00000078761; ENSMUSG00000019878. [P38533-2]
    GeneIDi15500.
    KEGGimmu:15500.
    UCSCiuc007fck.2. mouse. [P38533-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61754 mRNA. Translation: CAA43893.1 .
    AF045627
    , AF045615 , AF045616 , AF045617 , AF045618 , AF045619 , AF045620 , AF045621 , AF045622 , AF045623 , AF045624 , AF045625 , AF045626 Genomic DNA. Translation: AAD02417.1 .
    S79629 mRNA. Translation: AAB35307.1 .
    BC018414 mRNA. Translation: AAH18414.1 .
    CCDSi CCDS23852.1. [P38533-2 ]
    PIRi B40583.
    RefSeqi NP_032323.3. NM_008297.3. [P38533-2 ]
    UniGenei Mm.261395.
    Mm.447362.

    3D structure databases

    ProteinModelPortali P38533.
    SMRi P38533. Positions 1-113.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P38533.

    Proteomic databases

    PRIDEi P38533.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000079833 ; ENSMUSP00000078761 ; ENSMUSG00000019878 . [P38533-2 ]
    GeneIDi 15500.
    KEGGi mmu:15500.
    UCSCi uc007fck.2. mouse. [P38533-2 ]

    Organism-specific databases

    CTDi 3298.
    MGIi MGI:96239. Hsf2.

    Phylogenomic databases

    eggNOGi COG5169.
    GeneTreei ENSGT00390000001182.
    HOGENOMi HOG000253917.
    HOVERGENi HBG005999.
    InParanoidi P38533.
    KOi K09415.
    OMAi PKTDKQL.
    OrthoDBi EOG7353WM.
    PhylomeDBi P38533.
    TreeFami TF330401.

    Miscellaneous databases

    NextBioi 288394.
    PROi P38533.
    SOURCEi Search...

    Gene expression databases

    Bgeei P38533.
    CleanExi MM_HSF2.
    Genevestigatori P38533.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR027712. HSF2.
    IPR000232. HSF_DNA-bd.
    IPR027725. HSF_fam.
    IPR010542. Vert_HSTF_C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10015. PTHR10015. 1 hit.
    PTHR10015:SF144. PTHR10015:SF144. 1 hit.
    Pfami PF00447. HSF_DNA-bind. 1 hit.
    PF06546. Vert_HS_TF. 1 hit.
    [Graphical view ]
    PRINTSi PR00056. HSFDOMAIN.
    SMARTi SM00415. HSF. 1 hit.
    [Graphical view ]
    PROSITEi PS00434. HSF_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA-binding ability."
      Sarge K.D., Zimarino V., Holm K., Wu C., Morimoto R.I.
      Genes Dev. 5:1902-1911(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA).
      Strain: BALB/c.
      Tissue: Liver.
    2. "Genomic structure and chromosomal localization of the mouse Hsf2 gene and promoter sequences."
      Manuel M., Sage J., Mattei M.-G., Morange M., Mezger V.
      Gene 232:115-124(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM ALPHA).
      Strain: 129.
    3. "Tissue-dependent expression of heat shock factor 2 isoforms with distinct transcriptional activities."
      Goodson M.L., Park-Sarge O.-K., Sarge K.D.
      Mol. Cell. Biol. 15:5288-5293(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: CBA/J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).

    Entry informationi

    Entry nameiHSF2_MOUSE
    AccessioniPrimary (citable) accession number: P38533
    Secondary accession number(s): Q64157, Q8VEJ0, Q9Z2L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: August 30, 2002
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3