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P38523

- GRPE_YEAST

UniProt

P38523 - GRPE_YEAST

Protein

GrpE protein homolog, mitochondrial

Gene

MGE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of SSC1 to substrate proteins and the association of SSC1 with TIM44.1 Publication

    GO - Molecular functioni

    1. adenyl-nucleotide exchange factor activity Source: SGD

    GO - Biological processi

    1. protein import into mitochondrial matrix Source: SGD
    2. protein refolding Source: SGD
    3. regulation of catalytic activity Source: GOC

    Keywords - Molecular functioni

    Chaperone

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33730-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GrpE protein homolog, mitochondrial
    Gene namesi
    Name:MGE1
    Synonyms:GRPE, GRPE1, YGE1
    Ordered Locus Names:YOR232W
    ORF Names:O5099
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR232w.
    SGDiS000005758. MGE1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: Reactome
    2. mitochondrial matrix Source: SGD
    3. presequence translocase-associated import motor Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 228GrpE protein homolog, mitochondrialPRO_0000013046
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    MaxQBiP38523.
    PaxDbiP38523.
    PeptideAtlasiP38523.

    Expressioni

    Gene expression databases

    GenevestigatoriP38523.

    Interactioni

    Subunit structurei

    Component of the PAM complex, at least composed of SSC1 (mtHsp70), MGE1, TIM44, PAM16/TIM16, PAM17 and PAM18/TIM14. Interacts with SSQ1.2 Publications

    Protein-protein interaction databases

    BioGridi34625. 112 interactions.
    DIPiDIP-775N.
    IntActiP38523. 12 interactions.
    MINTiMINT-493356.
    STRINGi4932.YOR232W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38523.
    SMRiP38523. Positions 58-227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GrpE family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0576.
    GeneTreeiENSGT00390000005589.
    HOGENOMiHOG000252083.
    KOiK03687.
    OMAiPMEGKEP.
    OrthoDBiEOG7966TJ.

    Family and domain databases

    Gene3Di2.30.22.10. 1 hit.
    3.90.20.20. 1 hit.
    HAMAPiMF_01151. GrpE.
    InterProiIPR000740. GrpE.
    IPR013805. GrpE_coiled_coil.
    IPR009012. GrpE_head.
    [Graphical view]
    PANTHERiPTHR21237. PTHR21237. 1 hit.
    PfamiPF01025. GrpE. 1 hit.
    [Graphical view]
    PRINTSiPR00773. GRPEPROTEIN.
    SUPFAMiSSF51064. SSF51064. 1 hit.
    PROSITEiPS01071. GRPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38523-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRAFSAATVR ATTRKSFIPM APRTPFVTPS FTKNVGSMRR MRFYSDEAKS    50
    EESKENNEDL TEEQSEIKKL ESQLSAKTKE ASELKDRLLR SVADFRNLQQ 100
    VTKKDIQKAK DFALQKFAKD LLESVDNFGH ALNAFKEEDL QKSKEISDLY 150
    TGVRMTRDVF ENTLRKHGIE KLDPLGEPFD PNKHEATFEL PQPDKEPGTV 200
    FHVQQLGFTL NDRVIRPAKV GIVKGEEN 228
    Length:228
    Mass (Da):26,066
    Last modified:October 1, 1994 - v1
    Checksum:i816A5E26C648E613
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78350 Genomic DNA. Translation: CAA55145.1.
    D26059 Genomic DNA. Translation: BAA05058.1.
    U09565 Unassigned RNA. Translation: AAA19253.1.
    Z75140 Genomic DNA. Translation: CAA99452.1.
    BK006948 Genomic DNA. Translation: DAA11002.1.
    PIRiS41760.
    RefSeqiNP_014875.3. NM_001183651.3.

    Genome annotation databases

    EnsemblFungiiYOR232W; YOR232W; YOR232W.
    GeneIDi854407.
    KEGGisce:YOR232W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78350 Genomic DNA. Translation: CAA55145.1 .
    D26059 Genomic DNA. Translation: BAA05058.1 .
    U09565 Unassigned RNA. Translation: AAA19253.1 .
    Z75140 Genomic DNA. Translation: CAA99452.1 .
    BK006948 Genomic DNA. Translation: DAA11002.1 .
    PIRi S41760.
    RefSeqi NP_014875.3. NM_001183651.3.

    3D structure databases

    ProteinModelPortali P38523.
    SMRi P38523. Positions 58-227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34625. 112 interactions.
    DIPi DIP-775N.
    IntActi P38523. 12 interactions.
    MINTi MINT-493356.
    STRINGi 4932.YOR232W.

    Protein family/group databases

    TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Proteomic databases

    MaxQBi P38523.
    PaxDbi P38523.
    PeptideAtlasi P38523.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR232W ; YOR232W ; YOR232W .
    GeneIDi 854407.
    KEGGi sce:YOR232W.

    Organism-specific databases

    CYGDi YOR232w.
    SGDi S000005758. MGE1.

    Phylogenomic databases

    eggNOGi COG0576.
    GeneTreei ENSGT00390000005589.
    HOGENOMi HOG000252083.
    KOi K03687.
    OMAi PMEGKEP.
    OrthoDBi EOG7966TJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33730-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 976592.
    PROi P38523.

    Gene expression databases

    Genevestigatori P38523.

    Family and domain databases

    Gene3Di 2.30.22.10. 1 hit.
    3.90.20.20. 1 hit.
    HAMAPi MF_01151. GrpE.
    InterProi IPR000740. GrpE.
    IPR013805. GrpE_coiled_coil.
    IPR009012. GrpE_head.
    [Graphical view ]
    PANTHERi PTHR21237. PTHR21237. 1 hit.
    Pfami PF01025. GrpE. 1 hit.
    [Graphical view ]
    PRINTSi PR00773. GRPEPROTEIN.
    SUPFAMi SSF51064. SSF51064. 1 hit.
    PROSITEi PS01071. GRPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A mitochondrial homolog of bacterial GrpE interacts with mitochondrial hsp70 and is essential for viability."
      Bolliger L., Deloche O., Glick B.S., Georgopoulos C., Jenoe P., Kronidou N., Horst M., Morishima N., Schatz G.
      EMBO J. 13:1998-2006(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 50-66; 91-96 AND 109-116.
    2. "YGE1 is a yeast homologue of Escherichia coli grpE and is required for maintenance of mitochondrial functions."
      Ikeda E., Yoshida S., Mitsuzawa H., Uno I., Toh-e A.
      FEBS Lett. 339:265-268(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26786 / X2180-1A.
    3. Erratum
      Ikeda E., Yoshida S., Mitsuzawa H., Uno I., Toh-e A.
      FEBS Lett. 343:181-181(1994)
    4. "A role for a eukaryotic GrpE-related protein, Mge1p, in protein translocation."
      Laloraya S., Gambill D.B., Craig E.A.
      Proc. Natl. Acad. Sci. U.S.A. 91:6481-6485(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 204508 / S288c.
    5. "Sequence and analysis of a 26.9 kb fragment from chromosome XV of the yeast Saccharomyces cerevisiae."
      Boyer J., Michaux G., Fairhead C., Gaillon L., Dujon B.
      Yeast 12:1575-1586(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    6. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    8. "The role of the GrpE homologue, Mge1p, in mediating protein import and protein folding in mitochondria."
      Westermann B., Prip-Buus C., Neupert W., Schwarz E.
      EMBO J. 14:3452-3460(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The mitochondrial proteins Ssq1 and Jac1 are required for the assembly of iron sulfur clusters in mitochondria."
      Lutz T., Westermann B., Neupert W., Herrmann J.M.
      J. Mol. Biol. 307:815-825(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SSQ1.
    10. "Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor."
      van der Laan M., Chacinska A., Lind M., Perschil I., Sickmann A., Meyer H.E., Guiard B., Meisinger C., Pfanner N., Rehling P.
      Mol. Cell. Biol. 25:7449-7458(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE PAM COMPLEX WITH PAM16; PAM17; PAM18; TIM44 AND SSC1.

    Entry informationi

    Entry nameiGRPE_YEAST
    AccessioniPrimary (citable) accession number: P38523
    Secondary accession number(s): D6W2T6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3