P38507 (SPA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Immunoglobulin G-binding protein A
Short name=IgG-binding protein A

Alternative name(s):
Staphylococcal protein A

Gene names

Name:

spa

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted › cell wall; Peptidoglycan-anchor Potential.
Miscellaneous	Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.
Sequence similarities	Contains 1 LysM repeat.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Ligand	IgG-binding protein
PTM	Peptidoglycan-anchor
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular component	cell surface Inferred from electronic annotation. Source: InterPro cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: InterPro
Molecular function	IgG binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 36

36

Potential

Chain

37 – 477

441

Immunoglobulin G-binding protein A

PRO_0000005655

Propeptide

478 – 508

31

Removed by sortase Potential

PRO_0000005656

Regions

Repeat

35 – 88

54

B 1

Repeat

96 – 149

54

B 2

Repeat

154 – 207

54

B 3

Repeat

212 – 265

54

B 4

Repeat

266 – 323

58

B 5

Repeat

333 – 340

8

2-1

Repeat

341 – 348

8

2-2

Repeat

349 – 356

8

2-3

Repeat

357 – 364

8

2-4

Repeat

365 – 372

8

2-5

Repeat

373 – 380

8

2-6

Repeat

381 – 388

8

2-7

Repeat

389 – 396

8

2-8

Repeat

397 – 405

9

2-9

Repeat

406 – 413

8

2-10

Repeat

414 – 458

45

LysM

Repeat

414 – 421

8

2-11

Repeat

422 – 429

8

2-12

Region

333 – 408

76

12 X 8 AA approximate tandem repeats

Motif

474 – 478

5

LPXTG sorting signal Potential

Amino acid modifications

Modified residue

477

1

Pentaglycyl murein peptidoglycan amidated threonine Potential

Experimental info

Sequence conflict

273

1

K → D AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

508

Helix Strand Turn

Details...

Helix

41 – 51

11

Helix

58 – 70

13

Helix

72 – 74

3

Helix

75 – 88

14

Helix

217 – 227

11

Helix

235 – 247

13

Helix

252 – 265

14

Sequences

	Sequence		Length	Mass (Da)
	P38507 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: E78C538D4B5E88F5	FASTA	508	55,439
	10 20 30 40 50 60 MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV LNMPNLNADQ 70 80 90 100 110 120 RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNKF NKDQQSAFYE ILNMPNLNEE 130 140 150 160 170 180 QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ APKADNNFNK EQQNAFYEIL NMPNLNEEQR 190 200 210 220 230 240 NGFIQSLKDD PSQSANLLAE AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG 250 260 270 280 290 300 FIQSLKDDPS QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI 310 320 330 340 350 360 QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDGN KPGKEDGNKP GKEDNKKPGK 370 380 390 400 410 420 EDGNKPGKED NKKPGKEDGN KPGKEDGNKP GKEDGNKPGK EDGNKPGKED GNGVHVVKPG 430 440 450 460 470 480 DTVNDIAKAN GTTADKIAAD NKLADKNMIK PGQELVVDKK QPANHADANK AQALPETGEE 490 500 NPFIGTTVFG GLSLALGAAL LAGRRREL

References

[1]	"Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli." Shuttleworth H.L., Duggleby C.J., Jones S.A., Atkinson T., Minton N.P. Gene 58:283-295(1987) [PubMed: 2828190] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
[2]	"Structural studies on the four repetitive Fc-binding regions in protein A from Staphylococcus aureus." Sjoedahl J. Eur. J. Biochem. 78:471-490(1977) [PubMed: 913410] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
[3]	"Three-dimensional solution structure of the B domain of staphylococcal protein A: comparisons of the solution and crystal structures." Gouda H., Torigoe H., Saito A., Sato M., Arata Y., Shimada I. Biochemistry 31:9665-9672(1992) [PubMed: 1390743] [Abstract] Cited for: STRUCTURE BY NMR OF 211-270.
[4]	"Solution structure of the E-domain of staphylococcal protein A." Starovasnik M.A., Skelton N.J., O'Connell M.P., Kelley R.F., Reilly D., Fairbrother W.J. Biochemistry 35:15558-15569(1996) [PubMed: 8952510] [Abstract] Cited for: STRUCTURE BY NMR OF 37-92.
[5]	"High-resolution solution NMR structure of the Z domain of staphylococcal protein A." Tashiro M., Tejero R., Zimmerman D.E., Celda B., Nilsson B., Montelione G.T. J. Mol. Biol. 272:573-590(1997) [PubMed: 9325113] [Abstract] Cited for: STRUCTURE BY NMR OF 212-269.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M18264 Genomic DNA. Translation: AAA26677.1.

PIR

A29605.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BDC	NMR	-	A	212-270	[»]
1BDD	NMR	-	A	212-270	[»]
1EDI	NMR	-	A	37-92	[»]
1EDJ	NMR	-	A	37-92	[»]
1EDK	NMR	-	A	37-92	[»]
1EDL	NMR	-	A	37-92	[»]
1FC2	X-ray	2.80	C	212-269	[»]
1H0T	NMR	-	A	213-269	[»]
1LP1	X-ray	2.30	B	213-269	[»]
1Q2N	NMR	-	A	213-269	[»]
1SS1	NMR	-	A	212-270	[»]
2JWD	NMR	-	A	213-269	[»]
2SPZ	NMR	-	A	213-269	[»]
3MZW	X-ray	2.90	B	213-269	[»]

ProteinModelPortal

P38507.

SMR

P38507. Positions 37-92, 100-327.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR005877. Gpos_YSIRK.
IPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR019931. LPXTG-motif_cell_wall_anchor.
IPR005038. Octapeptide.
IPR018392. Peptidoglycan-bd_lysin.
IPR002482. Peptidoglycan-bd_Lysin_subgr.
IPR003132. Protein_A_Ig-bd.
IPR001899. Surface_protein_Gram_pos_cocci.
[Graphical view]

Pfam

PF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 11 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]

SMART

SM00257. LysM. 1 hit.
[Graphical view]

SUPFAM

SSF46997. Bac_Ig/alb_bind. 5 hits.

TIGRFAMs

TIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.

PROSITE

PS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

ProtoNet

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Entry information

Entry name

SPA_STAAU

Accession

Primary (citable) accession number: P38507

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	September 21, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program