Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P38507

- SPA_STAAU

UniProt

P38507 - SPA_STAAU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Ligandi

IgG-binding protein

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin G-binding protein A
Short name:
IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Gene namesi
Name:spa
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

GO - Cellular componenti

  1. cell wall Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3636Sequence AnalysisAdd
BLAST
Chaini37 – 477441Immunoglobulin G-binding protein APRO_0000005655Add
BLAST
Propeptidei478 – 50831Removed by sortasePROSITE-ProRule annotationPRO_0000005656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei477 – 4771Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PRIDEiP38507.

Interactioni

Protein-protein interaction databases

IntActiP38507. 1 interaction.
MINTiMINT-1505339.

Structurei

Secondary structure

1
508
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi41 – 5111
Beta strandi54 – 563
Helixi58 – 7013
Helixi72 – 743
Helixi75 – 8814
Helixi217 – 22711
Beta strandi231 – 2333
Helixi235 – 24713
Helixi249 – 2513
Helixi252 – 26514

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BDCNMR-A212-270[»]
1BDDNMR-A212-270[»]
1EDINMR-A37-92[»]
1EDJNMR-A37-92[»]
1EDKNMR-A37-92[»]
1EDLNMR-A37-92[»]
1FC2X-ray2.80C212-269[»]
1H0TNMR-A213-269[»]
1LP1X-ray2.30B212-269[»]
1Q2NNMR-A212-269[»]
1SS1NMR-A212-270[»]
2JWDNMR-A213-269[»]
2M5ANMR-A213-269[»]
2SPZNMR-A212-269[»]
3MZWX-ray2.90B212-269[»]
ProteinModelPortaliP38507.
SMRiP38507. Positions 37-92, 100-327.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38507.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati35 – 8854B 1Add
BLAST
Repeati96 – 14954B 2Add
BLAST
Repeati154 – 20754B 3Add
BLAST
Repeati212 – 26554B 4Add
BLAST
Repeati266 – 32358B 5Add
BLAST
Repeati333 – 34082-1
Repeati341 – 34882-2
Repeati349 – 35682-3
Repeati357 – 36482-4
Repeati365 – 37282-5
Repeati373 – 38082-6
Repeati381 – 38882-7
Repeati389 – 39682-8
Repeati397 – 40592-9
Repeati406 – 41382-10
Repeati414 – 45845LysMAdd
BLAST
Repeati414 – 42182-11
Repeati422 – 42982-12

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni333 – 4087612 X 8 AA approximate tandem repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi474 – 4785LPXTG sorting signalPROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 LysM repeat.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.

Family and domain databases

Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR019931. LPXTG_anchor.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 11 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38507-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV
60 70 80 90 100
LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNKF
110 120 130 140 150
NKDQQSAFYE ILNMPNLNEE QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ
160 170 180 190 200
APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLAE
210 220 230 240 250
AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS
260 270 280 290 300
QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI
310 320 330 340 350
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDGN KPGKEDGNKP
360 370 380 390 400
GKEDNKKPGK EDGNKPGKED NKKPGKEDGN KPGKEDGNKP GKEDGNKPGK
410 420 430 440 450
EDGNKPGKED GNGVHVVKPG DTVNDIAKAN GTTADKIAAD NKLADKNMIK
460 470 480 490 500
PGQELVVDKK QPANHADANK AQALPETGEE NPFIGTTVFG GLSLALGAAL

LAGRRREL
Length:508
Mass (Da):55,439
Last modified:October 1, 1994 - v1
Checksum:iE78C538D4B5E88F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti273 – 2731K → D AA sequence (PubMed:913410)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M18264 Genomic DNA. Translation: AAA26677.1.
PIRiA29605.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M18264 Genomic DNA. Translation: AAA26677.1 .
PIRi A29605.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BDC NMR - A 212-270 [» ]
1BDD NMR - A 212-270 [» ]
1EDI NMR - A 37-92 [» ]
1EDJ NMR - A 37-92 [» ]
1EDK NMR - A 37-92 [» ]
1EDL NMR - A 37-92 [» ]
1FC2 X-ray 2.80 C 212-269 [» ]
1H0T NMR - A 213-269 [» ]
1LP1 X-ray 2.30 B 212-269 [» ]
1Q2N NMR - A 212-269 [» ]
1SS1 NMR - A 212-270 [» ]
2JWD NMR - A 213-269 [» ]
2M5A NMR - A 213-269 [» ]
2SPZ NMR - A 212-269 [» ]
3MZW X-ray 2.90 B 212-269 [» ]
ProteinModelPortali P38507.
SMRi P38507. Positions 37-92, 100-327.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P38507. 1 interaction.
MINTi MINT-1505339.

Proteomic databases

PRIDEi P38507.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi NOG12793.

Miscellaneous databases

EvolutionaryTracei P38507.
PROi P38507.

Family and domain databases

Gene3Di 3.10.350.10. 1 hit.
InterProi IPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR019931. LPXTG_anchor.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view ]
Pfami PF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 11 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view ]
SMARTi SM00257. LysM. 1 hit.
[Graphical view ]
SUPFAMi SSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli."
    Shuttleworth H.L., Duggleby C.J., Jones S.A., Atkinson T., Minton N.P.
    Gene 58:283-295(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
  2. "Structural studies on the four repetitive Fc-binding regions in protein A from Staphylococcus aureus."
    Sjoedahl J.
    Eur. J. Biochem. 78:471-490(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
  3. "Three-dimensional solution structure of the B domain of staphylococcal protein A: comparisons of the solution and crystal structures."
    Gouda H., Torigoe H., Saito A., Sato M., Arata Y., Shimada I.
    Biochemistry 31:9665-9672(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 211-270.
  4. Cited for: STRUCTURE BY NMR OF 37-92.
  5. "High-resolution solution NMR structure of the Z domain of staphylococcal protein A."
    Tashiro M., Tejero R., Zimmerman D.E., Celda B., Nilsson B., Montelione G.T.
    J. Mol. Biol. 272:573-590(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 212-269.

Entry informationi

Entry nameiSPA_STAAU
AccessioniPrimary (citable) accession number: P38507
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3