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P38507

- SPA_STAAU

UniProt

P38507 - SPA_STAAU

Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    GO - Biological processi

    1. pathogenesis Source: InterPro

    Keywords - Ligandi

    IgG-binding protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Immunoglobulin G-binding protein A
    Short name:
    IgG-binding protein A
    Alternative name(s):
    Staphylococcal protein A
    Gene namesi
    Name:spa
    OrganismiStaphylococcus aureus
    Taxonomic identifieri1280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

    Subcellular locationi

    Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW
    3. membrane Source: InterPro

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3636Sequence AnalysisAdd
    BLAST
    Chaini37 – 477441Immunoglobulin G-binding protein APRO_0000005655Add
    BLAST
    Propeptidei478 – 50831Removed by sortasePROSITE-ProRule annotationPRO_0000005656Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei477 – 4771Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

    Keywords - PTMi

    Peptidoglycan-anchor

    Proteomic databases

    PRIDEiP38507.

    Interactioni

    Protein-protein interaction databases

    IntActiP38507. 1 interaction.
    MINTiMINT-1505339.

    Structurei

    Secondary structure

    1
    508
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi41 – 5111
    Beta strandi54 – 563
    Helixi58 – 7013
    Helixi72 – 743
    Helixi75 – 8814
    Helixi217 – 22711
    Beta strandi231 – 2333
    Helixi235 – 24713
    Helixi249 – 2513
    Helixi252 – 26514

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BDCNMR-A212-270[»]
    1BDDNMR-A212-270[»]
    1EDINMR-A37-92[»]
    1EDJNMR-A37-92[»]
    1EDKNMR-A37-92[»]
    1EDLNMR-A37-92[»]
    1FC2X-ray2.80C212-269[»]
    1H0TNMR-A213-269[»]
    1LP1X-ray2.30B212-269[»]
    1Q2NNMR-A212-269[»]
    1SS1NMR-A212-270[»]
    2JWDNMR-A213-269[»]
    2M5ANMR-A213-269[»]
    2SPZNMR-A212-269[»]
    3MZWX-ray2.90B212-269[»]
    ProteinModelPortaliP38507.
    SMRiP38507. Positions 37-92, 100-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38507.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati35 – 8854B 1Add
    BLAST
    Repeati96 – 14954B 2Add
    BLAST
    Repeati154 – 20754B 3Add
    BLAST
    Repeati212 – 26554B 4Add
    BLAST
    Repeati266 – 32358B 5Add
    BLAST
    Repeati333 – 34082-1
    Repeati341 – 34882-2
    Repeati349 – 35682-3
    Repeati357 – 36482-4
    Repeati365 – 37282-5
    Repeati373 – 38082-6
    Repeati381 – 38882-7
    Repeati389 – 39682-8
    Repeati397 – 40592-9
    Repeati406 – 41382-10
    Repeati414 – 45845LysMAdd
    BLAST
    Repeati414 – 42182-11
    Repeati422 – 42982-12

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni333 – 4087612 X 8 AA approximate tandem repeatsAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi474 – 4785LPXTG sorting signalPROSITE-ProRule annotation

    Sequence similaritiesi

    Contains 1 LysM repeat.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.

    Family and domain databases

    Gene3Di3.10.350.10. 1 hit.
    InterProiIPR019948. Gram-positive_anchor.
    IPR009063. Ig/albumin-bd.
    IPR019931. LPXTG_anchor.
    IPR018392. LysM_dom.
    IPR005038. Octapeptide.
    IPR003132. Protein_A_Ig-bd.
    IPR005877. YSIRK_signal_dom.
    [Graphical view]
    PfamiPF02216. B. 5 hits.
    PF00746. Gram_pos_anchor. 1 hit.
    PF01476. LysM. 1 hit.
    PF03373. Octapeptide. 11 hits.
    PF04650. YSIRK_signal. 1 hit.
    [Graphical view]
    SMARTiSM00257. LysM. 1 hit.
    [Graphical view]
    SUPFAMiSSF46997. SSF46997. 5 hits.
    SSF54106. SSF54106. 1 hit.
    TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
    TIGR01168. YSIRK_signal. 1 hit.
    PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38507-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV    50
    LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNKF 100
    NKDQQSAFYE ILNMPNLNEE QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ 150
    APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLAE 200
    AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS 250
    QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI 300
    QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDGN KPGKEDGNKP 350
    GKEDNKKPGK EDGNKPGKED NKKPGKEDGN KPGKEDGNKP GKEDGNKPGK 400
    EDGNKPGKED GNGVHVVKPG DTVNDIAKAN GTTADKIAAD NKLADKNMIK 450
    PGQELVVDKK QPANHADANK AQALPETGEE NPFIGTTVFG GLSLALGAAL 500
    LAGRRREL 508
    Length:508
    Mass (Da):55,439
    Last modified:October 1, 1994 - v1
    Checksum:iE78C538D4B5E88F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti273 – 2731K → D AA sequence (PubMed:913410)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18264 Genomic DNA. Translation: AAA26677.1.
    PIRiA29605.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18264 Genomic DNA. Translation: AAA26677.1 .
    PIRi A29605.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BDC NMR - A 212-270 [» ]
    1BDD NMR - A 212-270 [» ]
    1EDI NMR - A 37-92 [» ]
    1EDJ NMR - A 37-92 [» ]
    1EDK NMR - A 37-92 [» ]
    1EDL NMR - A 37-92 [» ]
    1FC2 X-ray 2.80 C 212-269 [» ]
    1H0T NMR - A 213-269 [» ]
    1LP1 X-ray 2.30 B 212-269 [» ]
    1Q2N NMR - A 212-269 [» ]
    1SS1 NMR - A 212-270 [» ]
    2JWD NMR - A 213-269 [» ]
    2M5A NMR - A 213-269 [» ]
    2SPZ NMR - A 212-269 [» ]
    3MZW X-ray 2.90 B 212-269 [» ]
    ProteinModelPortali P38507.
    SMRi P38507. Positions 37-92, 100-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P38507. 1 interaction.
    MINTi MINT-1505339.

    Proteomic databases

    PRIDEi P38507.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi NOG12793.

    Miscellaneous databases

    EvolutionaryTracei P38507.
    PROi P38507.

    Family and domain databases

    Gene3Di 3.10.350.10. 1 hit.
    InterProi IPR019948. Gram-positive_anchor.
    IPR009063. Ig/albumin-bd.
    IPR019931. LPXTG_anchor.
    IPR018392. LysM_dom.
    IPR005038. Octapeptide.
    IPR003132. Protein_A_Ig-bd.
    IPR005877. YSIRK_signal_dom.
    [Graphical view ]
    Pfami PF02216. B. 5 hits.
    PF00746. Gram_pos_anchor. 1 hit.
    PF01476. LysM. 1 hit.
    PF03373. Octapeptide. 11 hits.
    PF04650. YSIRK_signal. 1 hit.
    [Graphical view ]
    SMARTi SM00257. LysM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46997. SSF46997. 5 hits.
    SSF54106. SSF54106. 1 hit.
    TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
    TIGR01168. YSIRK_signal. 1 hit.
    PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli."
      Shuttleworth H.L., Duggleby C.J., Jones S.A., Atkinson T., Minton N.P.
      Gene 58:283-295(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
    2. "Structural studies on the four repetitive Fc-binding regions in protein A from Staphylococcus aureus."
      Sjoedahl J.
      Eur. J. Biochem. 78:471-490(1977) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
    3. "Three-dimensional solution structure of the B domain of staphylococcal protein A: comparisons of the solution and crystal structures."
      Gouda H., Torigoe H., Saito A., Sato M., Arata Y., Shimada I.
      Biochemistry 31:9665-9672(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 211-270.
    4. Cited for: STRUCTURE BY NMR OF 37-92.
    5. "High-resolution solution NMR structure of the Z domain of staphylococcal protein A."
      Tashiro M., Tejero R., Zimmerman D.E., Celda B., Nilsson B., Montelione G.T.
      J. Mol. Biol. 272:573-590(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 212-269.

    Entry informationi

    Entry nameiSPA_STAAU
    AccessioniPrimary (citable) accession number: P38507
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3