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Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

IgG-binding protein

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin G-binding protein A
Short name:
IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Gene namesi
Name:spa
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus

Subcellular locationi

  • Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3636Sequence AnalysisAdd
BLAST
Chaini37 – 477441Immunoglobulin G-binding protein APRO_0000005655Add
BLAST
Propeptidei478 – 50831Removed by sortasePROSITE-ProRule annotationPRO_0000005656Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei477 – 4771Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PRIDEiP38507.

Interactioni

Protein-protein interaction databases

IntActiP38507. 1 interaction.
MINTiMINT-1505339.

Structurei

Secondary structure

1
508
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi41 – 5111Combined sources
Beta strandi54 – 563Combined sources
Helixi58 – 7013Combined sources
Helixi72 – 743Combined sources
Helixi75 – 8814Combined sources
Helixi160 – 17112Combined sources
Helixi177 – 18913Combined sources
Helixi191 – 1933Combined sources
Helixi194 – 20714Combined sources
Helixi218 – 22912Combined sources
Beta strandi231 – 2333Combined sources
Helixi235 – 24713Combined sources
Helixi249 – 2513Combined sources
Helixi252 – 26514Combined sources
Helixi276 – 28712Combined sources
Helixi293 – 30513Combined sources
Helixi307 – 3093Combined sources
Helixi310 – 32314Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BDCNMR-A212-270[»]
1BDDNMR-A212-270[»]
1EDINMR-A37-92[»]
1EDJNMR-A37-92[»]
1EDKNMR-A37-92[»]
1EDLNMR-A37-92[»]
1FC2X-ray2.80C212-269[»]
1H0TNMR-A213-269[»]
1LP1X-ray2.30B212-269[»]
1Q2NNMR-A212-269[»]
1SS1NMR-A212-270[»]
2JWDNMR-A213-269[»]
2M5ANMR-A213-269[»]
2SPZNMR-A212-269[»]
3MZWX-ray2.90B212-269[»]
4NPDX-ray0.90A270-327[»]
4NPEX-ray1.42A270-327[»]
4NPFX-ray1.49X/Y154-269[»]
ProteinModelPortaliP38507.
SMRiP38507. Positions 37-92, 100-327.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38507.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati35 – 8854B 1Add
BLAST
Repeati96 – 14954B 2Add
BLAST
Repeati154 – 20754B 3Add
BLAST
Repeati212 – 26554B 4Add
BLAST
Repeati266 – 32358B 5Add
BLAST
Repeati333 – 34082-1
Repeati341 – 34882-2
Repeati349 – 35682-3
Repeati357 – 36482-4
Repeati365 – 37282-5
Repeati373 – 38082-6
Repeati381 – 38882-7
Repeati389 – 39682-8
Repeati397 – 40592-9
Repeati406 – 41382-10
Repeati414 – 45845LysMAdd
BLAST
Repeati414 – 42182-11
Repeati422 – 42982-12

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni333 – 4087612 X 8 AA approximate tandem repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi474 – 4785LPXTG sorting signalPROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 LysM repeat.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.

Family and domain databases

Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR019931. LPXTG_anchor.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 11 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV
60 70 80 90 100
LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNKF
110 120 130 140 150
NKDQQSAFYE ILNMPNLNEE QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ
160 170 180 190 200
APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLAE
210 220 230 240 250
AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS
260 270 280 290 300
QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI
310 320 330 340 350
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDGN KPGKEDGNKP
360 370 380 390 400
GKEDNKKPGK EDGNKPGKED NKKPGKEDGN KPGKEDGNKP GKEDGNKPGK
410 420 430 440 450
EDGNKPGKED GNGVHVVKPG DTVNDIAKAN GTTADKIAAD NKLADKNMIK
460 470 480 490 500
PGQELVVDKK QPANHADANK AQALPETGEE NPFIGTTVFG GLSLALGAAL

LAGRRREL
Length:508
Mass (Da):55,439
Last modified:October 1, 1994 - v1
Checksum:iE78C538D4B5E88F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti273 – 2731K → D AA sequence (PubMed:913410).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18264 Genomic DNA. Translation: AAA26677.1.
PIRiA29605.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18264 Genomic DNA. Translation: AAA26677.1.
PIRiA29605.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BDCNMR-A212-270[»]
1BDDNMR-A212-270[»]
1EDINMR-A37-92[»]
1EDJNMR-A37-92[»]
1EDKNMR-A37-92[»]
1EDLNMR-A37-92[»]
1FC2X-ray2.80C212-269[»]
1H0TNMR-A213-269[»]
1LP1X-ray2.30B212-269[»]
1Q2NNMR-A212-269[»]
1SS1NMR-A212-270[»]
2JWDNMR-A213-269[»]
2M5ANMR-A213-269[»]
2SPZNMR-A212-269[»]
3MZWX-ray2.90B212-269[»]
4NPDX-ray0.90A270-327[»]
4NPEX-ray1.42A270-327[»]
4NPFX-ray1.49X/Y154-269[»]
ProteinModelPortaliP38507.
SMRiP38507. Positions 37-92, 100-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP38507. 1 interaction.
MINTiMINT-1505339.

Proteomic databases

PRIDEiP38507.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiNOG12793.

Miscellaneous databases

EvolutionaryTraceiP38507.
PROiP38507.

Family and domain databases

Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR019931. LPXTG_anchor.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 11 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli."
    Shuttleworth H.L., Duggleby C.J., Jones S.A., Atkinson T., Minton N.P.
    Gene 58:283-295(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
  2. "Structural studies on the four repetitive Fc-binding regions in protein A from Staphylococcus aureus."
    Sjoedahl J.
    Eur. J. Biochem. 78:471-490(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
  3. "Three-dimensional solution structure of the B domain of staphylococcal protein A: comparisons of the solution and crystal structures."
    Gouda H., Torigoe H., Saito A., Sato M., Arata Y., Shimada I.
    Biochemistry 31:9665-9672(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 211-270.
  4. Cited for: STRUCTURE BY NMR OF 37-92.
  5. "High-resolution solution NMR structure of the Z domain of staphylococcal protein A."
    Tashiro M., Tejero R., Zimmerman D.E., Celda B., Nilsson B., Montelione G.T.
    J. Mol. Biol. 272:573-590(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 212-269.

Entry informationi

Entry nameiSPA_STAAU
AccessioniPrimary (citable) accession number: P38507
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 7, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.