Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38507 (SPA_STAAU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Immunoglobulin G-binding protein A

Short name=IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Gene names
Name:spa
OrganismStaphylococcus aureus
Taxonomic identifier1280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secretedcell wall; Peptidoglycan-anchor Potential.

Miscellaneous

Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.

Sequence similarities

Contains 1 LysM repeat.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3636 Potential
Chain37 – 477441Immunoglobulin G-binding protein A
PRO_0000005655
Propeptide478 – 50831Removed by sortase Potential
PRO_0000005656

Regions

Repeat35 – 8854B 1
Repeat96 – 14954B 2
Repeat154 – 20754B 3
Repeat212 – 26554B 4
Repeat266 – 32358B 5
Repeat333 – 34082-1
Repeat341 – 34882-2
Repeat349 – 35682-3
Repeat357 – 36482-4
Repeat365 – 37282-5
Repeat373 – 38082-6
Repeat381 – 38882-7
Repeat389 – 39682-8
Repeat397 – 40592-9
Repeat406 – 41382-10
Repeat414 – 45845LysM
Repeat414 – 42182-11
Repeat422 – 42982-12
Region333 – 4087612 X 8 AA approximate tandem repeats
Motif474 – 4785LPXTG sorting signal Potential

Amino acid modifications

Modified residue4771Pentaglycyl murein peptidoglycan amidated threonine Potential

Experimental info

Sequence conflict2731K → D AA sequence Ref.2

Secondary structure

................... 508
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38507 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: E78C538D4B5E88F5

FASTA50855,439
        10         20         30         40         50         60 
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV LNMPNLNADQ 

        70         80         90        100        110        120 
RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNKF NKDQQSAFYE ILNMPNLNEE 

       130        140        150        160        170        180 
QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ APKADNNFNK EQQNAFYEIL NMPNLNEEQR 

       190        200        210        220        230        240 
NGFIQSLKDD PSQSANLLAE AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG 

       250        260        270        280        290        300 
FIQSLKDDPS QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI 

       310        320        330        340        350        360 
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDGN KPGKEDGNKP GKEDNKKPGK 

       370        380        390        400        410        420 
EDGNKPGKED NKKPGKEDGN KPGKEDGNKP GKEDGNKPGK EDGNKPGKED GNGVHVVKPG 

       430        440        450        460        470        480 
DTVNDIAKAN GTTADKIAAD NKLADKNMIK PGQELVVDKK QPANHADANK AQALPETGEE 

       490        500 
NPFIGTTVFG GLSLALGAAL LAGRRREL 

« Hide

References

[1]"Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli."
Shuttleworth H.L., Duggleby C.J., Jones S.A., Atkinson T., Minton N.P.
Gene 58:283-295(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
[2]"Structural studies on the four repetitive Fc-binding regions in protein A from Staphylococcus aureus."
Sjoedahl J.
Eur. J. Biochem. 78:471-490(1977) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 12598 / Cowan 1 / DSM 20372 / NCIMB 11787 / NCTC 8530.
[3]"Three-dimensional solution structure of the B domain of staphylococcal protein A: comparisons of the solution and crystal structures."
Gouda H., Torigoe H., Saito A., Sato M., Arata Y., Shimada I.
Biochemistry 31:9665-9672(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 211-270.
[4]"Solution structure of the E-domain of staphylococcal protein A."
Starovasnik M.A., Skelton N.J., O'Connell M.P., Kelley R.F., Reilly D., Fairbrother W.J.
Biochemistry 35:15558-15569(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 37-92.
[5]"High-resolution solution NMR structure of the Z domain of staphylococcal protein A."
Tashiro M., Tejero R., Zimmerman D.E., Celda B., Nilsson B., Montelione G.T.
J. Mol. Biol. 272:573-590(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 212-269.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M18264 Genomic DNA. Translation: AAA26677.1.
PIRA29605.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BDCNMR-A212-270[»]
1BDDNMR-A212-270[»]
1EDINMR-A37-92[»]
1EDJNMR-A37-92[»]
1EDKNMR-A37-92[»]
1EDLNMR-A37-92[»]
1FC2X-ray2.80C212-269[»]
1H0TNMR-A213-269[»]
1LP1X-ray2.30B212-269[»]
1Q2NNMR-A212-269[»]
1SS1NMR-A212-270[»]
2JWDNMR-A213-269[»]
2M5ANMR-A213-269[»]
2SPZNMR-A212-269[»]
3MZWX-ray2.90B212-269[»]
ProteinModelPortalP38507.
SMRP38507. Positions 37-92, 100-327.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP38507. 1 interaction.
MINTMINT-1505339.

Proteomic databases

PRIDEP38507.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGNOG12793.

Family and domain databases

Gene3D3.10.350.10. 1 hit.
InterProIPR005877. Gpos_YSIRK.
IPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR019931. LPXTG_anchor.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
[Graphical view]
PfamPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 11 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEPS50847. GRAM_POS_ANCHORING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38507.
PROP38507.

Entry information

Entry nameSPA_STAAU
AccessionPrimary (citable) accession number: P38507
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references