Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

IgG-binding protein

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin G-binding protein A
Short name:
IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Gene namesi
Name:spa
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
ChainiPRO_000000565537 – 477Immunoglobulin G-binding protein AAdd BLAST441
PropeptideiPRO_0000005656478 – 508Removed by sortasePROSITE-ProRule annotationAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei477Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation1

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PRIDEiP38507.

Interactioni

Protein-protein interaction databases

IntActiP38507. 1 interactor.
MINTiMINT-1505339.
STRINGi93062.SACOL0095.

Structurei

Secondary structure

1508
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi41 – 51Combined sources11
Beta strandi54 – 56Combined sources3
Helixi58 – 70Combined sources13
Helixi72 – 74Combined sources3
Helixi75 – 88Combined sources14
Helixi101 – 112Combined sources12
Helixi119 – 131Combined sources13
Helixi133 – 135Combined sources3
Helixi136 – 147Combined sources12
Helixi160 – 171Combined sources12
Helixi177 – 189Combined sources13
Helixi191 – 193Combined sources3
Helixi194 – 207Combined sources14
Helixi218 – 229Combined sources12
Beta strandi231 – 233Combined sources3
Helixi235 – 247Combined sources13
Helixi249 – 251Combined sources3
Helixi252 – 265Combined sources14
Helixi276 – 287Combined sources12
Beta strandi289 – 291Combined sources3
Helixi293 – 305Combined sources13
Helixi307 – 309Combined sources3
Helixi310 – 323Combined sources14
Beta strandi335 – 337Combined sources3
Helixi339 – 348Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BDCNMR-A212-270[»]
1BDDNMR-A212-270[»]
1EDINMR-A37-92[»]
1EDJNMR-A37-92[»]
1EDKNMR-A37-92[»]
1EDLNMR-A37-92[»]
1FC2X-ray2.80C212-269[»]
1H0TNMR-A213-269[»]
1LP1X-ray2.30B212-269[»]
1Q2NNMR-A212-269[»]
1SS1NMR-A212-270[»]
2JWDNMR-A213-269[»]
2M5ANMR-A213-269[»]
2SPZNMR-A212-269[»]
3MZWX-ray2.90B212-269[»]
4NPDX-ray0.90A270-327[»]
4NPEX-ray1.42A270-327[»]
4NPFX-ray1.49X/Y154-269[»]
4WWIX-ray2.31A/B/C270-327[»]
4Y4YX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d158-211[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d219-459[»]
4Y5ZX-ray2.950/1/2/3/4/5/6/7/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V158-211[»]
0/1/2/3/4/5/6/7/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V219-459[»]
4ZMDX-ray1.87A/B270-327[»]
4ZNCX-ray2.28A/B/C270-327[»]
5CBNX-ray2.30A217-269[»]
5CBOX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L101-153[»]
5COCX-ray2.67A213-267[»]
5EWXX-ray2.60A/B212-266[»]
ProteinModelPortaliP38507.
SMRiP38507.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38507.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati35 – 88B 1Add BLAST54
Repeati96 – 149B 2Add BLAST54
Repeati154 – 207B 3Add BLAST54
Repeati212 – 265B 4Add BLAST54
Repeati266 – 323B 5Add BLAST58
Repeati333 – 3402-18
Repeati341 – 3482-28
Repeati349 – 3562-38
Repeati357 – 3642-48
Repeati365 – 3722-58
Repeati373 – 3802-68
Repeati381 – 3882-78
Repeati389 – 3962-88
Repeati397 – 4052-99
Repeati406 – 4132-108
Domaini413 – 457LysMPROSITE-ProRule annotationAdd BLAST45
Repeati414 – 4212-118
Repeati422 – 4292-128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni333 – 40812 X 8 AA approximate tandem repeatsAdd BLAST76

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi474 – 478LPXTG sorting signalPROSITE-ProRule annotation5

Sequence similaritiesi

Contains 1 LysM domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4106I9N. Bacteria.
ENOG410XXBQ. LUCA.

Family and domain databases

CDDicd00118. LysM. 1 hit.
Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 11 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51782. LYSM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV
60 70 80 90 100
LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNKF
110 120 130 140 150
NKDQQSAFYE ILNMPNLNEE QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ
160 170 180 190 200
APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLAE
210 220 230 240 250
AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS
260 270 280 290 300
QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI
310 320 330 340 350
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDGN KPGKEDGNKP
360 370 380 390 400
GKEDNKKPGK EDGNKPGKED NKKPGKEDGN KPGKEDGNKP GKEDGNKPGK
410 420 430 440 450
EDGNKPGKED GNGVHVVKPG DTVNDIAKAN GTTADKIAAD NKLADKNMIK
460 470 480 490 500
PGQELVVDKK QPANHADANK AQALPETGEE NPFIGTTVFG GLSLALGAAL

LAGRRREL
Length:508
Mass (Da):55,439
Last modified:October 1, 1994 - v1
Checksum:iE78C538D4B5E88F5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti273K → D AA sequence (PubMed:913410).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18264 Genomic DNA. Translation: AAA26677.1.
PIRiA29605.
RefSeqiWP_047211818.1. NZ_JYAJ01000001.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18264 Genomic DNA. Translation: AAA26677.1.
PIRiA29605.
RefSeqiWP_047211818.1. NZ_JYAJ01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BDCNMR-A212-270[»]
1BDDNMR-A212-270[»]
1EDINMR-A37-92[»]
1EDJNMR-A37-92[»]
1EDKNMR-A37-92[»]
1EDLNMR-A37-92[»]
1FC2X-ray2.80C212-269[»]
1H0TNMR-A213-269[»]
1LP1X-ray2.30B212-269[»]
1Q2NNMR-A212-269[»]
1SS1NMR-A212-270[»]
2JWDNMR-A213-269[»]
2M5ANMR-A213-269[»]
2SPZNMR-A212-269[»]
3MZWX-ray2.90B212-269[»]
4NPDX-ray0.90A270-327[»]
4NPEX-ray1.42A270-327[»]
4NPFX-ray1.49X/Y154-269[»]
4WWIX-ray2.31A/B/C270-327[»]
4Y4YX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d158-211[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d219-459[»]
4Y5ZX-ray2.950/1/2/3/4/5/6/7/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V158-211[»]
0/1/2/3/4/5/6/7/A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V219-459[»]
4ZMDX-ray1.87A/B270-327[»]
4ZNCX-ray2.28A/B/C270-327[»]
5CBNX-ray2.30A217-269[»]
5CBOX-ray2.80A/B/C/D/E/F/G/H/I/J/K/L101-153[»]
5COCX-ray2.67A213-267[»]
5EWXX-ray2.60A/B212-266[»]
ProteinModelPortaliP38507.
SMRiP38507.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP38507. 1 interactor.
MINTiMINT-1505339.
STRINGi93062.SACOL0095.

Proteomic databases

PRIDEiP38507.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4106I9N. Bacteria.
ENOG410XXBQ. LUCA.

Miscellaneous databases

EvolutionaryTraceiP38507.
PROiP38507.

Family and domain databases

CDDicd00118. LysM. 1 hit.
Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 11 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51782. LYSM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPA_STAAU
AccessioniPrimary (citable) accession number: P38507
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.