Reviewed, UniProtKB/Swiss-Prot P38500 (NIR_BETVE)

Names and origin

Protein names

Recommended name:
    Ferredoxin--nitrite reductase, chloroplastic
    EC=1.7.7.1

Gene names

Name:

NIR1

Organism

Betula verrucosa (White birch) (Betula pendula)

Taxonomic identifier

3505 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fagales › Betulaceae › Betula

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	NH3 + 2 H2O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H+.
Cofactor	Binds 1 siroheme per subunit. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast By similarity.
Induction	By nitrate.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ferredoxin-nitrite reductase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 22	22	Chloroplast Potential
	Chain	23 – 583	561	Ferredoxin--nitrite reductase, chloroplastic		PRO_0000019704
Sites
	Metal binding	461	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	467	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	502	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	506	1	Iron (siroheme axial ligand) By similarity
	Metal binding	506	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38500-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 482111EF7E66991F	FASTA	583	65,229
	10 20 30 40 50 60 MSSLSVRFLS PPLFSSTPAW PRTGLAATQA VPPVVAEVDA GRLEPRVEER EGYWVLKEKF 70 80 90 100 110 120 REGINPQEKL KLEREPMKLF MEGGIEDLAK MSLEEIDKDK ISKSDIDVRL KWLGLFHRRK 130 140 150 160 170 180 HHYGRFMMRL KLPNGVTTSA QTRYLASVIR KYGKDGCADV TTRQNWQIRG VVLSDVPEIL 190 200 210 220 230 240 KGLDEVGLTS LQSGMDNVRN PVGNPLAGID IHEIVATRPY NNLLSQFITA NSRGNLAFTN 250 260 270 280 290 300 LPRKWNVCVV GSHDLFEHPH INDLAYMPAI KDGRFGFNLL VGGFFSPRRC AEAVPLDAWV 310 320 330 340 350 360 SADDIILVCK AILEAYRDLG TRGNRQKTRM MWLIDELGIE GFRSEVVKRM PNQELERAAP 370 380 390 400 410 420 EDLIEKQWER RELIGVHPQK QEGLSYVGLH IPVGRVQADD MDELARLADT YGCGELRLTV 430 440 450 460 470 480 EQNIIIPNIE NSKLEALLGE PLLKDRFSPE PPILMKGLVA CTGNQFCGQA IIETKARALK 490 500 510 520 530 540 VTEEVQRQVA VTRPVRMHWT GCPNSCGQVQ VADIGFMGCM ARDENGKPCE GAAVFLGGRI 550 560 570 580 GSDSHLGNLY KKGVPCKNLV PLVVDILVKH FGAVPREREE SED

References

[1]	"Sequence of a cDNA encoding nitrite reductase from the tree Betula pendula and identification of conserved protein regions." Friemann A., Brinkmann K., Hachtel W. Mol. Gen. Genet. 231:411-416(1992) [PubMed: 1347145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60093 mRNA. Translation: CAA42690.1.
PIR	S20495.
3D structure databases
SMR	P38500. Positions 42-576.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.7.1. 228863.
Family and domain databases
InterPro	IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIR_BETVE

Accession

Primary (citable) accession number: P38500

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)