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Protein

Oxygen-insensitive NAD(P)H nitroreductase

Gene

nfsB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species.1 Publication

Catalytic activityi

A 5,6,7,8-tetrahydropteridine + NAD(P)+ = a 6,7-dihydropteridine + NAD(P)H.

Cofactori

Enzyme regulationi

Subject to competitive inhibition by dicoumarol, nicotinic acid and acetate with respect to NADH. Subject to uncompetitive inhibition by dicoumarol, nicotinic acid and acetate with respect to nitrofurazone and nitrofurantoin.1 Publication

Kineticsi

  1. KM=350 µM for NADH1 Publication
  2. KM=1850 µM for nitrofurazone1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei41NAD or NADP; via amide nitrogen1
    Binding sitei71FMN3 Publications1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi10 – 14FMN3 Publications5
    Nucleotide bindingi153 – 158NAD or NADPBy similarity6
    Nucleotide bindingi165 – 166FMN3 Publications2
    Nucleotide bindingi205 – 207FMN3 Publications3

    GO - Molecular functioni

    • 6,7-dihydropteridine reductase activity Source: EcoCyc
    • FMN binding Source: EcoCyc
    • NAD(P)H nitroreductase activity Source: EcoCyc

    GO - Biological processi

    • 2,4,6-trinitrotoluene catabolic process Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Flavoprotein, FMN, NAD, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:DIHYDROPTERIREDUCT-MONOMER.
    ECOL316407:JW0567-MONOMER.
    MetaCyc:DIHYDROPTERIREDUCT-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oxygen-insensitive NAD(P)H nitroreductase (EC:1.-.-.-)
    Alternative name(s):
    Dihydropteridine reductase (EC:1.5.1.34)
    FMN-dependent nitroreductase
    Gene namesi
    Name:nfsB1 Publication
    Synonyms:dprA, nfnB1 Publication, nfsI, ntr
    Ordered Locus Names:b0578, JW0567
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG20151. nfsB.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: UniProtKB
    • membrane Source: UniProtKB
    Complete GO annotation...

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL1075080.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000727111 – 217Oxygen-insensitive NAD(P)H nitroreductaseAdd BLAST217

    Proteomic databases

    EPDiP38489.
    PaxDbiP38489.
    PRIDEiP38489.

    2D gel databases

    SWISS-2DPAGEP38489.

    Interactioni

    Subunit structurei

    Homodimer.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-909296,EBI-909296

    Protein-protein interaction databases

    BioGridi4262913. 3 interactors.
    DIPiDIP-10330N.
    IntActiP38489. 12 interactors.
    STRINGi511145.b0578.

    Structurei

    Secondary structure

    1217
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 9Combined sources7
    Beta strandi14 – 16Combined sources3
    Helixi24 – 36Combined sources13
    Helixi40 – 42Combined sources3
    Beta strandi46 – 51Combined sources6
    Helixi54 – 61Combined sources8
    Helixi62 – 64Combined sources3
    Helixi66 – 71Combined sources6
    Helixi72 – 77Combined sources6
    Beta strandi78 – 89Combined sources12
    Helixi92 – 104Combined sources13
    Helixi111 – 130Combined sources20
    Helixi135 – 156Combined sources22
    Helixi169 – 176Combined sources8
    Helixi178 – 181Combined sources4
    Beta strandi183 – 192Combined sources10
    Helixi199 – 201Combined sources3
    Helixi210 – 213Combined sources4
    Beta strandi214 – 217Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1DS7X-ray2.06A/B1-217[»]
    1ICRX-ray1.70A/B1-217[»]
    1ICUX-ray1.80A/B/C/D1-217[»]
    1ICVX-ray2.40A/B/C/D1-217[»]
    1IDTX-ray2.00A/B1-217[»]
    1OO5X-ray2.50A/B1-217[»]
    1OO6X-ray2.00A/B1-217[»]
    1OONX-ray2.49A/B1-217[»]
    1OOQX-ray2.00A/B1-217[»]
    1YKIX-ray1.70A/B/C/D1-217[»]
    1YLRX-ray1.70A/B1-217[»]
    1YLUX-ray2.00A/B1-217[»]
    3X21X-ray3.00A/B/C/D/E/F/G/H/I/J1-217[»]
    3X22X-ray2.00A/B1-217[»]
    ProteinModelPortaliP38489.
    SMRiP38489.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38489.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the nitroreductase family.Curated

    Phylogenomic databases

    eggNOGiENOG4108RCM. Bacteria.
    COG0778. LUCA.
    HOGENOMiHOG000146740.
    InParanoidiP38489.
    KOiK10679.
    OMAiCLAEEGL.
    PhylomeDBiP38489.

    Family and domain databases

    CDDicd02149. NfsB_like_nitroreductase. 1 hit.
    Gene3Di3.40.109.10. 1 hit.
    InterProiIPR033878. NfsB-like.
    IPR029479. Nitroreductase.
    IPR000415. Nitroreductase-like.
    [Graphical view]
    PfamiPF00881. Nitroreductase. 1 hit.
    [Graphical view]
    SUPFAMiSSF55469. SSF55469. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38489-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV
    60 70 80 90 100
    ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD
    110 120 130 140 150
    QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF
    160 170 180 190 200
    LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN
    210
    ATLPKSRLPQ NITLTEV
    Length:217
    Mass (Da):23,905
    Last modified:October 1, 1994 - v1
    Checksum:iA516CEFC3D46AEAC
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti5S → C AA sequence (PubMed:3060113).Curated1
    Sequence conflicti10 – 12RHS → CIV AA sequence (PubMed:3060113).Curated3
    Sequence conflicti19S → M AA sequence (PubMed:3060113).Curated1
    Sequence conflicti21Missing AA sequence (PubMed:1472094).Curated1
    Sequence conflicti28E → D AA sequence (PubMed:1472094).Curated1
    Sequence conflicti180E → I AA sequence (PubMed:1472094).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D25414 Genomic DNA. Translation: BAA05004.1.
    U07860 Genomic DNA. Translation: AAC43263.1.
    U82598 Genomic DNA. Translation: AAB40776.1.
    U00096 Genomic DNA. Translation: AAC73679.1.
    AP009048 Genomic DNA. Translation: BAA35218.1.
    PIRiI67685.
    S01818.
    RefSeqiNP_415110.1. NC_000913.3.
    WP_000351487.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73679; AAC73679; b0578.
    BAA35218; BAA35218; BAA35218.
    GeneIDi945778.
    KEGGiecj:JW0567.
    eco:b0578.
    PATRICi32116324. VBIEscCol129921_0602.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D25414 Genomic DNA. Translation: BAA05004.1.
    U07860 Genomic DNA. Translation: AAC43263.1.
    U82598 Genomic DNA. Translation: AAB40776.1.
    U00096 Genomic DNA. Translation: AAC73679.1.
    AP009048 Genomic DNA. Translation: BAA35218.1.
    PIRiI67685.
    S01818.
    RefSeqiNP_415110.1. NC_000913.3.
    WP_000351487.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1DS7X-ray2.06A/B1-217[»]
    1ICRX-ray1.70A/B1-217[»]
    1ICUX-ray1.80A/B/C/D1-217[»]
    1ICVX-ray2.40A/B/C/D1-217[»]
    1IDTX-ray2.00A/B1-217[»]
    1OO5X-ray2.50A/B1-217[»]
    1OO6X-ray2.00A/B1-217[»]
    1OONX-ray2.49A/B1-217[»]
    1OOQX-ray2.00A/B1-217[»]
    1YKIX-ray1.70A/B/C/D1-217[»]
    1YLRX-ray1.70A/B1-217[»]
    1YLUX-ray2.00A/B1-217[»]
    3X21X-ray3.00A/B/C/D/E/F/G/H/I/J1-217[»]
    3X22X-ray2.00A/B1-217[»]
    ProteinModelPortaliP38489.
    SMRiP38489.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262913. 3 interactors.
    DIPiDIP-10330N.
    IntActiP38489. 12 interactors.
    STRINGi511145.b0578.

    Chemistry databases

    ChEMBLiCHEMBL1075080.

    2D gel databases

    SWISS-2DPAGEP38489.

    Proteomic databases

    EPDiP38489.
    PaxDbiP38489.
    PRIDEiP38489.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73679; AAC73679; b0578.
    BAA35218; BAA35218; BAA35218.
    GeneIDi945778.
    KEGGiecj:JW0567.
    eco:b0578.
    PATRICi32116324. VBIEscCol129921_0602.

    Organism-specific databases

    EchoBASEiEB4146.
    EcoGeneiEG20151. nfsB.

    Phylogenomic databases

    eggNOGiENOG4108RCM. Bacteria.
    COG0778. LUCA.
    HOGENOMiHOG000146740.
    InParanoidiP38489.
    KOiK10679.
    OMAiCLAEEGL.
    PhylomeDBiP38489.

    Enzyme and pathway databases

    BioCyciEcoCyc:DIHYDROPTERIREDUCT-MONOMER.
    ECOL316407:JW0567-MONOMER.
    MetaCyc:DIHYDROPTERIREDUCT-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP38489.
    PROiP38489.

    Family and domain databases

    CDDicd02149. NfsB_like_nitroreductase. 1 hit.
    Gene3Di3.40.109.10. 1 hit.
    InterProiIPR033878. NfsB-like.
    IPR029479. Nitroreductase.
    IPR000415. Nitroreductase-like.
    [Graphical view]
    PfamiPF00881. Nitroreductase. 1 hit.
    [Graphical view]
    SUPFAMiSSF55469. SSF55469. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiNFSB_ECOLI
    AccessioniPrimary (citable) accession number: P38489
    Secondary accession number(s): P19575
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: November 30, 2016
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.