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Protein

Galectin-3

Gene

LGALS3

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIgE-binding protein
Biological processDifferentiation, Immunity, Innate immunity, mRNA processing, mRNA splicing
LigandLectin

Names & Taxonomyi

Protein namesi
Recommended name:
Galectin-3
Short name:
Gal-3
Alternative name(s):
35 kDa lectin
Carbohydrate-binding protein 35
Short name:
CBP 35
Galactose-specific lectin 3
IgE-binding protein
Laminin-binding protein
Lectin L-29
Mac-2 antigen
Gene namesi
Name:LGALS3
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Secreted By similarity

  • Note: Secreted by a non-classical secretory pathway and associates with the cell surface.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000769282 – 296Galectin-3Add BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei6Phosphoserine; by CK11 Publication1
Modified residuei12Phosphoserine; by CK11 Publication1
Disulfide bondi219InterchainBy similarity

Post-translational modificationi

The degree of phosphorylation is higher in the cytoplasmic form than in the nuclear form. In protein isolated from a canine kidney cell line, 90% of the phosphate was on Ser-6 and 10% was on Ser-12.1 Publication

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP38486.
PRIDEiP38486.

PTM databases

iPTMnetiP38486.

Interactioni

Subunit structurei

Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000022105.

Structurei

3D structure databases

ProteinModelPortaliP38486.
SMRiP38486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati36 – 4419
Repeati45 – 5329
Repeati54 – 6239
Repeati63 – 7149
Repeati72 – 8059
Repeati81 – 8969
Repeati90 – 9879
Repeati99 – 10789
Repeati108 – 1159; approximate8
Repeati116 – 124109
Repeati125 – 13411; approximate10
Repeati135 – 14312; approximate9
Domaini164 – 294GalectinPROSITE-ProRule annotationAdd BLAST131

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 14312 X 9 AA tandem repeats of Y-P-G-X(3)-P-G-[GAT]Add BLAST108
Regioni227 – 233Beta-galactoside bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi272 – 287Nuclear export signalBy similarityAdd BLAST16

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3587. Eukaryota.
ENOG4111EA0. LUCA.
HOGENOMiHOG000246423.
HOVERGENiHBG006255.
InParanoidiP38486.

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR015534. Galectin_3.
IPR001079. Galectin_CRD.
PANTHERiPTHR11346:SF145. PTHR11346:SF145. 1 hit.
PfamiView protein in Pfam
PF00337. Gal-bind_lectin. 1 hit.
SMARTiView protein in SMART
SM00908. Gal-bind_lectin. 1 hit.
SM00276. GLECT. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS51304. GALECTIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38486-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSFSLNDA LSGSGNPNPQ GWPGPWGNQP AGAGGYPGAS YPGAYPGQAP
60 70 80 90 100
PGGYPGQAPP GGYPGQAPPG GYPGQAPPGG YPGQAPPGGY PGQAPPGGYP
110 120 130 140 150
GQAPPGTYPG PTAPAYPGPT APGTQPGQPS GPGAYPPPGQ PSAPGAYPAA
160 170 180 190 200
GPFGIPAGPL TVPYDLPLPG GVKPRMLITI LGTVRPSANR LALDFKRGND
210 220 230 240 250
VAFHFNPRFN EDNKRVIVCN TKLDNIWGKE ERQAAFPFES GKPFKIQVLV
260 270 280 290
ESDHFKVAVN DAHLLQYNHR MKNLPEISKL GISGDIDLTS ASYAMI
Length:296
Mass (Da):30,330
Last modified:January 23, 2007 - v3
Checksum:iFB1DD61EF1444AEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23429 mRNA. Translation: AAA16211.1.
PIRiA49688.
UniGeneiCfa.797.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLEG3_CANLF
AccessioniPrimary (citable) accession number: P38486
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: May 10, 2017
This is version 115 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome