P38448 (ALKH_DICD3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
KHG/KDPG aldolase

Including the following 2 domains:

1. 4-hydroxy-2-oxoglutarate aldolase
   EC=4.1.3.16
   Alternative name(s):
   2-keto-4-hydroxyglutarate aldolase
   Short name=KHG-aldolase
2. 2-dehydro-3-deoxy-phosphogluconate aldolase
   EC=4.1.2.14
   Alternative name(s):
   2-keto-3-deoxy-6-phosphogluconate aldolase
   Short name=KDPG-aldolase
   Phospho-2-dehydro-3-deoxygluconate aldolase
   Phospho-2-keto-3-deoxygluconate aldolase

Gene names

Name:	eda
Synonyms:	kdgA
Ordered Locus Names:	Dda3937_03573

Organism

Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) [Complete proteome] [HAMAP]

Taxonomic identifier

198628 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya › Dickeya dadantii

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate. 2-dehydro-3-deoxy-D-gluconate 6-phosphate = pyruvate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2. Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the KHG/KDPG aldolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2-dehydro-3-deoxy-phosphogluconate aldolase activity Inferred from electronic annotation. Source: EC 4-hydroxy-2-oxoglutarate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	KHG/KDPG aldolase		PRO_0000201040
Sites
	Active site	45	1	By similarity
	Active site	49	1	By similarity
	Active site	133	1	Schiff-base intermediate with KHG or pyruvate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38448 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 641A0D1015CD7413	FASTA	213	22,184
	10 20 30 40 50 60 MKNWKTSAEQ ILTAGPVVPV IVINKLEHAV PMAKALVAGG VRVLELTLRT ECAVEAIRLI 70 80 90 100 110 120 AQEVPDAIVG AGTVTNPQQL AEVTAAGAQF AISPGLTEPL LKAATEGTIP LIPGISTVSE 130 140 150 160 170 180 LMLGMDYGLR EFKFFPAEAN GGVKALQAIA GPFGKIRFCP TGGISLKNYR DYLALKSVLC 190 200 210 VGGSWLVPAD ALESGDYDRI TALAREAVAG ATA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of the Erwinia chrysanthemi region containing the kdgA and zwf genes." Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. Mol. Microbiol. 11:67-75(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 3937.
[2]	"Genome sequence of the plant-pathogenic bacterium Dickeya dadantii 3937." Glasner J.D., Yang C.H., Reverchon S., Hugouvieux-Cotte-Pattat N., Condemine G., Bohin J.P., Van Gijsegem F., Yang S., Franza T., Expert D., Plunkett G. III, San Francisco M.J., Charkowski A.O., Py B., Bell K., Rauscher L., Rodriguez-Palenzuela P., Toussaint A. , Holeva M.C., He S.Y., Douet V., Boccara M., Blanco C., Toth I., Anderson B.D., Biehl B.S., Mau B., Flynn S.M., Barras F., Lindeberg M., Birch P.R., Tsuyumu S., Shi X., Hibbing M., Yap M.N., Carpentier M., Dassa E., Umehara M., Kim J.F., Rusch M., Soni P., Mayhew G.F., Fouts D.E., Gill S.R., Blattner F.R., Keen N.T., Perna N.T. J. Bacteriol. 193:2076-2077(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 3937.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74866 Genomic DNA. Translation: CAA52859.1. CP002038 Genomic DNA. Translation: ADM98359.1.
PIR	S37054.
RefSeq	YP_003882916.1. NC_014500.1.
3D structure databases
ProteinModelPortal	P38448.
SMR	P38448. Positions 1-211.
ModBase	Search...
Proteomic databases
PRIDE	P38448.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADM98359; ADM98359; Dda3937_03573.
GeneID	9733589.
KEGG	ddd:Dda3937_03573.
PATRIC	42316624. VBIDicDad25310_2135.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000233114.
KO	K01625.
OMA	AQFAVSP.
Enzyme and pathway databases
BioCyc	DDAD198628:GHFQ-2203-MONOMER. MetaCyc:MONOMER-15645.
UniPathway	UPA00227. UPA00856; UER00829.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000887. Aldlse_KDPG_KHG. IPR013785. Aldolase_TIM. [Graphical view]
Pfam	PF01081. Aldolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01182. eda. 1 hit.
PROSITE	PS00159. ALDOLASE_KDPG_KHG_1. 1 hit. PS00160. ALDOLASE_KDPG_KHG_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALKH_DICD3

Accession

Primary (citable) accession number: P38448
Secondary accession number(s): E0SB90

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program