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Protein

Endonuclease G, mitochondrial

Gene

ENDOG

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei143 – 1431Proton acceptorPROSITE-ProRule annotation
Metal bindingi174 – 1741Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease G, mitochondrial (EC:3.1.30.-)
Short name:
Endo G
Gene namesi
Name:ENDOG
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848Mitochondrion1 PublicationAdd
BLAST
Chaini49 – 299251Endonuclease G, mitochondrialPRO_0000019917Add
BLAST

Proteomic databases

PaxDbiP38447.
PRIDEiP38447.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000016564.

Structurei

3D structure databases

ProteinModelPortaliP38447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3721. Eukaryota.
COG1864. LUCA.
GeneTreeiENSGT00530000063772.
HOGENOMiHOG000214172.
HOVERGENiHBG002851.
InParanoidiP38447.
KOiK01173.
OMAiSSCDFHE.
OrthoDBiEOG7VB2FZ.
TreeFamiTF105386.

Family and domain databases

Gene3Di3.40.570.10. 1 hit.
InterProiIPR018524. DNA/RNA_endonuclease_AS.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR020821. Extracellular_endonuc_su_A.
[Graphical view]
PfamiPF01223. Endonuclease_NS. 1 hit.
[Graphical view]
SMARTiSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
[Graphical view]
PROSITEiPS01070. NUCLEASE_NON_SPEC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38447-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLRAGLTL ALGAGLGAAA ESWWRQRADA RATPGLLSRL PVLPVAAAAG
60 70 80 90 100
LPAVPGAPAG GGPGELAKYG LPGVAQLKSR ASYVLCYDPR TRGALWVVEQ
110 120 130 140 150
LRPEGLRGDG NRSSCDFHED DSVHAYHRAT NADYRGSGFD RGHLAAAANH
160 170 180 190 200
RWSQKAMDDT FYLSNVAPQV PHLNQNAWNN LEKYSRSLTR TYQNVYVCTG
210 220 230 240 250
PLFLPRTEAD GKSYVKYQVI GKNHVAVPTH FFKVLILEAA GGQIELRSYV
260 270 280 290
MPNAPVDEAI PLEHFLVPIE SIERASGLLF VPNILARAGS LKAITAGSK
Length:299
Mass (Da):32,262
Last modified:October 1, 1994 - v1
Checksum:iB28FAFF56F04CFC7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti172 – 1721H → S AA sequence (PubMed:7870594).Curated
Sequence conflicti264 – 2641H → S AA sequence (PubMed:7870594).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72802 mRNA. Translation: CAA51320.1.
BC134634 mRNA. Translation: AAI34635.1.
RefSeqiNP_787017.1. NM_175823.3.
UniGeneiBt.103334.

Genome annotation databases

EnsembliENSBTAT00000016564; ENSBTAP00000016564; ENSBTAG00000012484.
GeneIDi327707.
KEGGibta:327707.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72802 mRNA. Translation: CAA51320.1.
BC134634 mRNA. Translation: AAI34635.1.
RefSeqiNP_787017.1. NM_175823.3.
UniGeneiBt.103334.

3D structure databases

ProteinModelPortaliP38447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000016564.

Proteomic databases

PaxDbiP38447.
PRIDEiP38447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000016564; ENSBTAP00000016564; ENSBTAG00000012484.
GeneIDi327707.
KEGGibta:327707.

Organism-specific databases

CTDi2021.

Phylogenomic databases

eggNOGiKOG3721. Eukaryota.
COG1864. LUCA.
GeneTreeiENSGT00530000063772.
HOGENOMiHOG000214172.
HOVERGENiHBG002851.
InParanoidiP38447.
KOiK01173.
OMAiSSCDFHE.
OrthoDBiEOG7VB2FZ.
TreeFamiTF105386.

Miscellaneous databases

NextBioi20810171.

Family and domain databases

Gene3Di3.40.570.10. 1 hit.
InterProiIPR018524. DNA/RNA_endonuclease_AS.
IPR001604. DNA/RNA_non-sp_Endonuclease.
IPR020821. Extracellular_endonuc_su_A.
[Graphical view]
PfamiPF01223. Endonuclease_NS. 1 hit.
[Graphical view]
SMARTiSM00892. Endonuclease_NS. 1 hit.
SM00477. NUC. 1 hit.
[Graphical view]
PROSITEiPS01070. NUCLEASE_NON_SPEC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primers for mitochondrial DNA replication generated by endonuclease G."
    Cote J., Ruiz-Carrillo A.
    Science 261:765-769(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.
  3. "Reproducible high yield sequencing of proteins electrophoretically separated and transferred to an inert support."
    Moos M. Jr., Nguyen N.Y., Liu T.-Y.
    J. Biol. Chem. 263:6005-6008(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 49-75.
    Tissue: Liver and Thymus.
  4. "Endonuclease G from mammalian nuclei is identical to the major endonuclease of mitochondria."
    Gerschenson M., Houmiel K.L., Low R.L.
    Nucleic Acids Res. 23:88-97(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    Tissue: Heart.

Entry informationi

Entry nameiNUCG_BOVIN
AccessioniPrimary (citable) accession number: P38447
Secondary accession number(s): A4IFL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: February 17, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site contains 1 hydrated divalent metal cation that has only 1 direct interaction with the protein; all other interactions are via water molecules.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.