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P38433

- ACE1_CAEEL

UniProt

P38433 - ACE1_CAEEL

Protein

Acetylcholinesterase 1

Gene

ace-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Rapidly hydrolyzes choline released into the synapse. It can hydrolyze butyrylthiocholine.

    Catalytic activityi

    Acetylcholine + H2O = choline + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei216 – 2161Acyl-ester intermediatePROSITE-ProRule annotation
    Active sitei346 – 3461Charge relay systemBy similarity
    Active sitei468 – 4681Charge relay systemBy similarity

    GO - Molecular functioni

    1. acetylcholinesterase activity Source: WormBase
    2. cholinesterase activity Source: RefGenome
    3. identical protein binding Source: WormBase

    GO - Biological processi

    1. acetylcholine catabolic process Source: WormBase
    2. acetylcholine catabolic process in synaptic cleft Source: WormBase
    3. choline metabolic process Source: RefGenome
    4. regulation of locomotion Source: WormBase
    5. synaptic transmission, cholinergic Source: RefGenome

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Keywords - Biological processi

    Neurotransmitter degradation

    Enzyme and pathway databases

    ReactomeiREACT_183754. Synthesis of PC.

    Protein family/group databases

    MEROPSiS09.979.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetylcholinesterase 1 (EC:3.1.1.7)
    Short name:
    AChE 1
    Gene namesi
    Name:ace-1
    ORF Names:W09B12.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome X

    Organism-specific databases

    WormBaseiW09B12.1; CE07569; WBGene00000035; ace-1.

    Subcellular locationi

    Cell junctionsynapse By similarity. Secreted By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity
    Note: May be secreted or membrane associated via a non-catalytic subunit.

    GO - Cellular componenti

    1. cell Source: WormBase
    2. cell junction Source: UniProtKB-KW
    3. extracellular region Source: WormBase
    4. membrane Source: WormBase
    5. plasma membrane Source: UniProtKB-SubCell
    6. synapse Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Secreted, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 620589Acetylcholinesterase 1PRO_0000008610Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi74 – 741N-linked (GlcNAc...)1 Publication
    Disulfide bondi82 ↔ 109By similarity
    Disulfide bondi270 ↔ 286By similarity
    Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi430 ↔ 558By similarity
    Glycosylationi486 – 4861N-linked (GlcNAc...)1 Publication
    Glycosylationi536 – 5361N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi618 – 618InterchainBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP38433.
    PRIDEiP38433.

    Expressioni

    Developmental stagei

    Detected at all stages. Found to be more abundant in larval stages than in embryos or adults.

    Interactioni

    Subunit structurei

    Oligomer composed of disulfide-linked homodimers.By similarity

    Protein-protein interaction databases

    STRINGi6239.W09B12.1.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP38433.
    SMRiP38433. Positions 19-571.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2272.
    GeneTreeiENSGT00740000115241.
    HOGENOMiHOG000091866.
    InParanoidiP38433.
    KOiK01049.
    OMAiWEAFADI.
    PhylomeDBiP38433.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view]
    PfamiPF00135. COesterase. 1 hit.
    [Graphical view]
    PRINTSiPR00878. CHOLNESTRASE.
    ProDomiPD415333. AChE_tetra. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38433-1 [UniParc]FASTAAdd to Basket

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    MRNSLLFFIF LPSTILAVDL IHLHDGSPLF GEEVLSQTGK PLTRFQGIPF    50
    AEPPVGNLRF KKPKPKQPWR IPLNATTPPN SCIQSEDTYF GDFYGSTMWN 100
    ANTKLSEDCL YLNVYVPGKV DPNKKLAVMV WVYGGGFWSG TATLDVYDGR 150
    ILTVEENVIL VAMNYRVSIF GFLYMNRPEA PGNMGMWDQL LAMKWVHKNI 200
    DLFGGDLSRI TLFGESAGAA SVSIHMLSPK SAPYFHRAII QSGSATSPWA 250
    IEPRDVALAR AVILYNAMKC GNMSLINPDY DRILDCFQRA DADALRENEW 300
    APVREFGDFP WVPVVDGDFL LENAQTSLKQ GNFKKTQLLA GSNRDESIYF 350
    LTYQLPDIFP VADFFTKTDF IKDRQLWIKG VKDLLPRQIL KCQLTLAAVL 400
    HEYEPQDLPV TPRDWINAMD KMLGDYHFTC SVNEMALAHT KHGGDTYYYY 450
    FTHRASQQTW PEWMGVLHGY EINFIFGEPL NQKRFNYTDE ERELSNRFMR 500
    YWANFAKTGD PNKNEDGSFT QDVWPKYNSV SMEYMNMTVE SSYPSMKRIG 550
    HGPRRKECAF WKAYLPNLMA AVADVGDPYL VWKQQMDKWQ NEYITDWQYH 600
    FEQYKRYQTY RQSDSETCGG 620
    Length:620
    Mass (Da):71,433
    Last modified:October 1, 1994 - v1
    Checksum:i61D78C4899F55C65
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75331 mRNA. Translation: CAA53080.1.
    FO081067 Genomic DNA. Translation: CCD68912.1.
    PIRiA54413.
    T29347.
    RefSeqiNP_510660.1. NM_078259.6.
    UniGeneiCel.19718.

    Genome annotation databases

    EnsemblMetazoaiW09B12.1.1; W09B12.1.1; WBGene00000035.
    W09B12.1.2; W09B12.1.2; WBGene00000035.
    GeneIDi181706.
    KEGGicel:CELE_W09B12.1.
    UCSCiW09B12.1.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75331 mRNA. Translation: CAA53080.1 .
    FO081067 Genomic DNA. Translation: CCD68912.1 .
    PIRi A54413.
    T29347.
    RefSeqi NP_510660.1. NM_078259.6.
    UniGenei Cel.19718.

    3D structure databases

    ProteinModelPortali P38433.
    SMRi P38433. Positions 19-571.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.W09B12.1.1.

    Protein family/group databases

    MEROPSi S09.979.

    Proteomic databases

    PaxDbi P38433.
    PRIDEi P38433.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai W09B12.1.1 ; W09B12.1.1 ; WBGene00000035 .
    W09B12.1.2 ; W09B12.1.2 ; WBGene00000035 .
    GeneIDi 181706.
    KEGGi cel:CELE_W09B12.1.
    UCSCi W09B12.1.1. c. elegans.

    Organism-specific databases

    CTDi 181706.
    WormBasei W09B12.1 ; CE07569 ; WBGene00000035 ; ace-1.

    Phylogenomic databases

    eggNOGi COG2272.
    GeneTreei ENSGT00740000115241.
    HOGENOMi HOG000091866.
    InParanoidi P38433.
    KOi K01049.
    OMAi WEAFADI.
    PhylomeDBi P38433.

    Enzyme and pathway databases

    Reactomei REACT_183754. Synthesis of PC.

    Miscellaneous databases

    NextBioi 915022.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR014788. AChE_tetra.
    IPR002018. CarbesteraseB.
    IPR019826. Carboxylesterase_B_AS.
    IPR019819. Carboxylesterase_B_CS.
    IPR000997. Cholinesterase.
    [Graphical view ]
    Pfami PF00135. COesterase. 1 hit.
    [Graphical view ]
    PRINTSi PR00878. CHOLNESTRASE.
    ProDomi PD415333. AChE_tetra. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00122. CARBOXYLESTERASE_B_1. 1 hit.
    PS00941. CARBOXYLESTERASE_B_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA sequence, gene structure, and in vitro expression of ace-1, the gene encoding acetylcholinesterase of class A in the nematode Caenorhabditis elegans."
      Arpagaus M., Fedon Y., Cousin X., Chatonnet A., Berge J.-B., Fournier D., Toutant J.-P.
      J. Biol. Chem. 269:9957-9965(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74 AND ASN-486, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiACE1_CAEEL
    AccessioniPrimary (citable) accession number: P38433
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3