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Protein

Acetylcholinesterase 1

Gene

ace-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Rapidly hydrolyzes choline released into the synapse. It can hydrolyze butyrylthiocholine.

Catalytic activityi

Acetylcholine + H2O = choline + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei216 – 2161Acyl-ester intermediatePROSITE-ProRule annotation
Active sitei346 – 3461Charge relay systemBy similarity
Active sitei468 – 4681Charge relay systemBy similarity

GO - Molecular functioni

  1. acetylcholinesterase activity Source: WormBase
  2. identical protein binding Source: WormBase

GO - Biological processi

  1. acetylcholine catabolic process Source: WormBase
  2. acetylcholine catabolic process in synaptic cleft Source: WormBase
  3. regulation of locomotion Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Neurotransmitter degradation

Enzyme and pathway databases

ReactomeiREACT_183754. Synthesis of PC.
REACT_259914. Neurotransmitter Clearance In The Synaptic Cleft.

Protein family/group databases

MEROPSiS09.979.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholinesterase 1 (EC:3.1.1.7)
Short name:
AChE 1
Gene namesi
Name:ace-1
ORF Names:W09B12.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome X

Organism-specific databases

WormBaseiW09B12.1; CE07569; WBGene00000035; ace-1.

Subcellular locationi

Cell junctionsynapse By similarity. Secreted By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity
Note: May be secreted or membrane associated via a non-catalytic subunit.

GO - Cellular componenti

  1. cell Source: WormBase
  2. cell junction Source: UniProtKB-KW
  3. extracellular region Source: WormBase
  4. extracellular space Source: GO_Central
  5. membrane Source: WormBase
  6. plasma membrane Source: UniProtKB-SubCell
  7. synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Secreted, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 620589Acetylcholinesterase 1PRO_0000008610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi74 – 741N-linked (GlcNAc...)1 Publication
Disulfide bondi82 ↔ 109By similarity
Disulfide bondi270 ↔ 286By similarity
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi430 ↔ 558By similarity
Glycosylationi486 – 4861N-linked (GlcNAc...)1 Publication
Glycosylationi536 – 5361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi618 – 618InterchainBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP38433.
PRIDEiP38433.

Expressioni

Developmental stagei

Detected at all stages. Found to be more abundant in larval stages than in embryos or adults.

Interactioni

Subunit structurei

Oligomer composed of disulfide-linked homodimers.By similarity

Protein-protein interaction databases

STRINGi6239.W09B12.1.1.

Structurei

3D structure databases

ProteinModelPortaliP38433.
SMRiP38433. Positions 35-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
InParanoidiP38433.
KOiK01049.
OMAiWEAFADI.
PhylomeDBiP38433.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38433-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRNSLLFFIF LPSTILAVDL IHLHDGSPLF GEEVLSQTGK PLTRFQGIPF
60 70 80 90 100
AEPPVGNLRF KKPKPKQPWR IPLNATTPPN SCIQSEDTYF GDFYGSTMWN
110 120 130 140 150
ANTKLSEDCL YLNVYVPGKV DPNKKLAVMV WVYGGGFWSG TATLDVYDGR
160 170 180 190 200
ILTVEENVIL VAMNYRVSIF GFLYMNRPEA PGNMGMWDQL LAMKWVHKNI
210 220 230 240 250
DLFGGDLSRI TLFGESAGAA SVSIHMLSPK SAPYFHRAII QSGSATSPWA
260 270 280 290 300
IEPRDVALAR AVILYNAMKC GNMSLINPDY DRILDCFQRA DADALRENEW
310 320 330 340 350
APVREFGDFP WVPVVDGDFL LENAQTSLKQ GNFKKTQLLA GSNRDESIYF
360 370 380 390 400
LTYQLPDIFP VADFFTKTDF IKDRQLWIKG VKDLLPRQIL KCQLTLAAVL
410 420 430 440 450
HEYEPQDLPV TPRDWINAMD KMLGDYHFTC SVNEMALAHT KHGGDTYYYY
460 470 480 490 500
FTHRASQQTW PEWMGVLHGY EINFIFGEPL NQKRFNYTDE ERELSNRFMR
510 520 530 540 550
YWANFAKTGD PNKNEDGSFT QDVWPKYNSV SMEYMNMTVE SSYPSMKRIG
560 570 580 590 600
HGPRRKECAF WKAYLPNLMA AVADVGDPYL VWKQQMDKWQ NEYITDWQYH
610 620
FEQYKRYQTY RQSDSETCGG
Length:620
Mass (Da):71,433
Last modified:October 1, 1994 - v1
Checksum:i61D78C4899F55C65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75331 mRNA. Translation: CAA53080.1.
FO081067 Genomic DNA. Translation: CCD68912.1.
PIRiA54413.
T29347.
RefSeqiNP_510660.1. NM_078259.6.
UniGeneiCel.19718.

Genome annotation databases

EnsemblMetazoaiW09B12.1.1; W09B12.1.1; WBGene00000035.
W09B12.1.2; W09B12.1.2; WBGene00000035.
GeneIDi181706.
KEGGicel:CELE_W09B12.1.
UCSCiW09B12.1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75331 mRNA. Translation: CAA53080.1.
FO081067 Genomic DNA. Translation: CCD68912.1.
PIRiA54413.
T29347.
RefSeqiNP_510660.1. NM_078259.6.
UniGeneiCel.19718.

3D structure databases

ProteinModelPortaliP38433.
SMRiP38433. Positions 35-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.W09B12.1.1.

Protein family/group databases

MEROPSiS09.979.

Proteomic databases

PaxDbiP38433.
PRIDEiP38433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiW09B12.1.1; W09B12.1.1; WBGene00000035.
W09B12.1.2; W09B12.1.2; WBGene00000035.
GeneIDi181706.
KEGGicel:CELE_W09B12.1.
UCSCiW09B12.1.1. c. elegans.

Organism-specific databases

CTDi181706.
WormBaseiW09B12.1; CE07569; WBGene00000035; ace-1.

Phylogenomic databases

eggNOGiCOG2272.
GeneTreeiENSGT00760000118946.
HOGENOMiHOG000091866.
InParanoidiP38433.
KOiK01049.
OMAiWEAFADI.
PhylomeDBiP38433.

Enzyme and pathway databases

ReactomeiREACT_183754. Synthesis of PC.
REACT_259914. Neurotransmitter Clearance In The Synaptic Cleft.

Miscellaneous databases

NextBioi915022.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR014788. AChE_tetra.
IPR002018. CarbesteraseB.
IPR019826. Carboxylesterase_B_AS.
IPR019819. Carboxylesterase_B_CS.
IPR000997. Cholinesterase.
[Graphical view]
PfamiPF00135. COesterase. 1 hit.
[Graphical view]
PRINTSiPR00878. CHOLNESTRASE.
ProDomiPD415333. AChE_tetra. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00122. CARBOXYLESTERASE_B_1. 1 hit.
PS00941. CARBOXYLESTERASE_B_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence, gene structure, and in vitro expression of ace-1, the gene encoding acetylcholinesterase of class A in the nematode Caenorhabditis elegans."
    Arpagaus M., Fedon Y., Cousin X., Chatonnet A., Berge J.-B., Fournier D., Toutant J.-P.
    J. Biol. Chem. 269:9957-9965(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-74 AND ASN-486, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiACE1_CAEEL
AccessioniPrimary (citable) accession number: P38433
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: February 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.