Reviewed, UniProtKB/Swiss-Prot P38432 (P80C_HUMAN)

Names and origin

Protein names

Recommended name:
    Coilin
Alternative name(s):
    p80

Gene names

Name:	COIL
Synonyms:	CLN80

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.
Subunit structure	Interacts with ANKS1B. Ref.7
Subcellular location	Nucleus. Note: Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
Tissue specificity	Found in all the cell types examined.
Sequence similarities	To Xenopus sphere organelles protein SPH-1.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	Cajal body Inferred from direct assay. Source: UniProtKB nucleolus Inferred from direct assay. Source: UniProtKB
Molecular function	protein C-terminus binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATXN1	P54253-1	5	EBI-945751,EBI-930975
LNX1	Q8TBB1	1	EBI-945751,EBI-739832

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Coilin		PRO_0000058146
Regions
	Repeat	223 – 226	4	1-1
	Repeat	268 – 271	4	1-2
	Repeat	386 – 389	4	2-1
	Repeat	413 – 414	2	3-1
	Repeat	415 – 416	2	3-2
	Repeat	417 – 418	2	3-3
	Repeat	419 – 420	2	3-4
	Repeat	517 – 520	4	2-2
	Region	223 – 271	49	2 X 4 AA repeats of A-R-N-S
	Region	386 – 520	135	2 X 4 AA repeats of S-L-P-A
	Region	413 – 420	8	4 X 2 AA tandem repeats of R-G
	Compositional bias	181 – 201	21	Lys-rich (basic)
	Compositional bias	243 – 252	10	Ser/Thr-rich
	Compositional bias	310 – 320	11	Ser/Thr-rich
Amino acid modifications
	Modified residue	122	1	Phosphothreonine Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15
	Modified residue	271	1	Phosphoserine Ref.13 Ref.15
	Modified residue	290	1	Phosphothreonine Ref.13
	Modified residue	301	1	Phosphoserine Ref.13
	Modified residue	303	1	Phosphothreonine Ref.13 Ref.15 Ref.8
	Modified residue	456	1	Phosphothreonine Ref.15
	Modified residue	487	1	Phosphoserine Ref.15
	Modified residue	489	1	Phosphoserine Ref.10 Ref.13 Ref.15
	Modified residue	566	1	Phosphoserine Ref.15
	Modified residue	568	1	Phosphoserine Ref.15

Sequences

	Sequence		Length	Mass (Da)
	P38432-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 879702B590F16849	FASTA	576	62,608
	10 20 30 40 50 60 MAASETVRLR LQFDYPPPAT PHCTAFWLLV DLNRCRVVTD LISLIRQRFG FSSGAFLGLY 70 80 90 100 110 120 LEGGLLPPAE SARLVRDNDC LRVKLEERGV AENSVVISNG DINLSLRKAK KRAFQLEEGE 130 140 150 160 170 180 ETEPDCKYSK KHWKSRENNN NNEKVLDLEP KAVTDQTVSK KNKRKNKATC GTVGDDNEEA 190 200 210 220 230 240 KRKSPKKKEK CEYKKKAKNP KSPKVQAVKD WANQRCSSPK GSARNSLVKA KRKGSVSVCS 250 260 270 280 290 300 KESPSSSSES ESCDESISDG PSKVTLEARN SSEKLPTELS KEEPSTKNTT ADKLAIKLGF 310 320 330 340 350 360 SLTPSKGKTS GTTSSSSDSS AESDDQCLMS SSTPECAAGF LKTVGLFAGR GRPGPGLSSQ 370 380 390 400 410 420 TAGAAGWRRS GSNGGGQAPG ASPSVSLPAS LGRGWGREEN LFSWKGAKGR GMRGRGRGRG 430 440 450 460 470 480 HPVSCVVNRS TDNQRQQQLN DVVKNSSTII QNPVETPKKD YSLLPLLAAA PQVGEKIAFK 490 500 510 520 530 540 LLELTSSYSP DVSDYKEGRI LSHNPETQQV DIEILSSLPA LREPGKFDLV YHNENGAEVV 550 560 570 EYAVTQESKI TVFWKELIDP RLIIESPSNT SSTEPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure, expression and chromosomal localization of human p80-coilin gene." Chan E.K.L., Takano S., Andrade L.E.C., Hamel J.C., Matera A.G. Nucleic Acids Res. 22:4462-4469(1994) [PubMed: 7971277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin." Andrade L.E.C., Chan E.K.L., Raska I., Peebles C.L., Roos G., Tan E.M. J. Exp. Med. 173:1407-1419(1991) [PubMed: 2033369] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 172-576. Tissue: Liver.
[6]	"Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis -- evidence that the coiled body is a kinetic nuclear structure." Carmo-Fonseca M., Ferreira J., Lamond A.I. J. Cell Biol. 120:841-852(1993) [PubMed: 7679389] [Abstract] Cited for: CHARACTERIZATION.
[7]	"A novel EB-1/AIDA-1 isoform, AIDA-1c, interacts with the Cajal body protein coilin." Xu H., Hebert M.D. BMC Cell Biol. 6:23-23(2005) [PubMed: 15862129] [Abstract] Cited for: INTERACTION WITH ANKS1B.
[8]	"Large-scale characterization of HeLa cell nuclear phosphoproteins." Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004) [PubMed: 15302935] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-303, MASS SPECTROMETRY. Tissue: Epithelium.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122 AND SER-489, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"Phosphoproteome analysis of the human mitotic spindle." Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R. Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006) [PubMed: 16565220] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122, MASS SPECTROMETRY. Tissue: Epithelium.
[12]	"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122, MASS SPECTROMETRY.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122; SER-271; THR-290; SER-301; THR-303 AND SER-489, MASS SPECTROMETRY.
[14]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[15]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-122; SER-271; THR-303; THR-456; SER-487; SER-489; SER-566 AND SER-568, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58411 mRNA. Translation: AAA36412.1. AK313616 mRNA. Translation: BAG36378.1. CH471109 Genomic DNA. Translation: EAW94522.1. BC010385 mRNA. Translation: AAH10385.1. U06632 mRNA. Translation: AAB81550.1.
IPI	IPI00006442.
PIR	S50113.
RefSeq	NP_004636.1.
UniGene	Hs.532795
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P38432. 49 interactions.
STRING	P38432.
PTM databases
PhosphoSite	P38432.
Proteomic databases
PeptideAtlas	P38432.
PRIDE	P38432.
Genome annotation databases
Ensembl	ENST00000240316; ENSP00000240316; ENSG00000121058; Homo sapiens. [Genome view]
GeneID	8161.
KEGG	hsa:8161.
UCSC	uc002iuu.1. human.
Organism-specific databases
CTD	8161.
GeneCards	GC17M052370.
H-InvDB	HIX0014015.
HGNC	HGNC:2184. COIL.
HPA	CAB017524.
MIM	600272. gene.
PharmGKB	PA26700.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18140.
HOGENOM	HBG278407.
HOVERGEN	P38432.
InParanoid	P38432.
OMA	RQRFGFS.
OrthoDB	EOG9HB162.
PhylomeDB	P38432.
Gene expression databases
ArrayExpress	P38432.
Bgee	P38432.
CleanEx	HS_COIL.
Genevestigator	P38432.
GermOnline	ENSG00000121058. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	30832.
SOURCE	Search...

Entry information

Entry name

P80C_HUMAN

Accession

Primary (citable) accession number: P38432
Secondary accession number(s): B2R931

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 19, 2010
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.