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Protein

Eukaryotic translation initiation factor 5

Gene

TIF5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). eIF-5 is essential for cell viability.1 Publication

Miscellaneous

Present with 48300 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTPSequence analysis8

GO - Molecular functioni

  • GDP-dissociation inhibitor activity Source: SGD
  • GTPase activator activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • translation initiation factor activity Source: SGD
  • translation initiation factor binding Source: SGD

GO - Biological processi

  • formation of cytoplasmic translation initiation complex Source: SGD
  • mature ribosome assembly Source: SGD
  • negative regulation of translational initiation Source: SGD
  • regulation of translational initiation Source: SGD

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34197-MONOMER
ReactomeiR-SCE-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5
Short name:
eIF-5
Gene namesi
Name:TIF5
Ordered Locus Names:YPR041W
ORF Names:YP3085.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR041W
SGDiS000006245 TIF5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi343 – 364LPKIL…EIMRF → APKAAVQAANNDAASAAEAA RA in TIF5-12A; Abolishes binding to SUI3 and NIP1. Add BLAST22
Mutagenesisi388 – 398FITWLETAESD → AATAAETAAAA in TIF5-7A; Abolishes binding to SUI3 and NIP1. 1 PublicationAdd BLAST11

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000077911 – 405Eukaryotic translation initiation factor 5Add BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170PhosphoserineCombined sources1
Modified residuei172PhosphoserineCombined sources1
Modified residuei191PhosphothreonineCombined sources1
Modified residuei228PhosphoserineCombined sources1
Modified residuei317PhosphothreonineCombined sources1
Modified residuei397PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP38431
PeptideAtlasiP38431
PRIDEiP38431

PTM databases

iPTMnetiP38431

Interactioni

Subunit structurei

Monomer. Interacts with NIP1 and SUI3. The factors eIF-1, eIF-2, eIF-3, TIF5/eIF-5 and methionyl-tRNAi form a multifactor complex (MFC) that may bind to the 40S ribosome. TIF32, NIP1 and TIF5/eIF-5 comprise a minimal 40S-ribosome-binding unit.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • translation initiation factor binding Source: SGD

Protein-protein interaction databases

BioGridi36218, 98 interactors
DIPiDIP-2303N
IntActiP38431, 40 interactors
MINTiP38431
STRINGi4932.YPR041W

Structurei

Secondary structure

1405
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi244 – 259Combined sources16
Helixi269 – 278Combined sources10
Helixi287 – 294Combined sources8
Helixi300 – 303Combined sources4
Helixi308 – 314Combined sources7
Helixi318 – 333Combined sources16
Helixi337 – 342Combined sources6
Helixi343 – 352Combined sources10
Helixi358 – 366Combined sources9
Beta strandi370 – 373Combined sources4
Helixi375 – 383Combined sources9
Helixi386 – 394Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FULX-ray1.50A/B/C/D/E/F241-405[»]
ProteinModelPortaliP38431
SMRiP38431
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38431

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini241 – 402W2PROSITE-ProRule annotationAdd BLAST162

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi152 – 156Poly-Lys5
Compositional biasi396 – 405Asp/Glu-rich (acidic)10

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000016478
HOGENOMiHOG000214327
InParanoidiP38431
KOiK03262
OMAiDDKFYRY
OrthoDBiEOG092C44YB

Family and domain databases

Gene3Di1.25.40.180, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR016021 MIF4-like_sf
IPR002735 Transl_init_fac_IF2/IF5
IPR016189 Transl_init_fac_IF2/IF5_N
IPR016190 Transl_init_fac_IF2/IF5_Zn-bd
IPR003307 W2_domain
PfamiView protein in Pfam
PF01873 eIF-5_eIF-2B, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00653 eIF2B_5, 1 hit
SM00515 eIF5C, 1 hit
SUPFAMiSSF100966 SSF100966, 1 hit
SSF48371 SSF48371, 1 hit
SSF75689 SSF75689, 1 hit
PROSITEiView protein in PROSITE
PS51363 W2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: P38431-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSINICRDNH DPFYRYKMPP IQAKVEGRGN GIKTAVLNVA DISHALNRPA
60 70 80 90 100
PYIVKYFGFE LGAQTSISVD KDRYLVNGVH EPAKLQDVLD GFINKFVLCG
110 120 130 140 150
SCKNPETEII ITKDNDLVRD CKACGKRTPM DLRHKLSSFI LKNPPDSVSG
160 170 180 190 200
SKKKKKAATA SANVRGGGLS ISDIAQGKSQ NAPSDGTGSS TPQHHDEDED
210 220 230 240 250
ELSRQIKAAA STLEDIEVKD DEWAVDMSEE AIRARAKELE VNSELTQLDE
260 270 280 290 300
YGEWILEQAG EDKENLPSDV ELYKKAAELD VLNDPKIGCV LAQCLFDEDI
310 320 330 340 350
VNEIAEHNAF FTKILVTPEY EKNFMGGIER FLGLEHKDLI PLLPKILVQL
360 370 380 390 400
YNNDIISEEE IMRFGTKSSK KFVPKEVSKK VRRAAKPFIT WLETAESDDD

EEDDE
Length:405
Mass (Da):45,261
Last modified:October 1, 1994 - v1
Checksum:i1A1DA563B4ED1B1F
GO
Isoform Short (identifier: P38431-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Note: Produced by alternative initiation at Met-18 of isoform Long.
Show »
Length:388
Mass (Da):43,107
Checksum:iD988447BA354E2AD
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0187241 – 17Missing in isoform Short. CuratedAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10840 Genomic DNA No translation available.
Z71255 Genomic DNA Translation: CAA94989.1
Z68111 Genomic DNA Translation: CAA92145.1
Z73616 Genomic DNA Translation: CAA97991.1
BK006949 Genomic DNA Translation: DAA11466.1
PIRiA46699
RefSeqiNP_015366.1, NM_001184138.1 [P38431-1]

Genome annotation databases

EnsemblFungiiYPR041W; YPR041W; YPR041W [P38431-1]
GeneIDi856154
KEGGisce:YPR041W

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiIF5_YEAST
AccessioniPrimary (citable) accession number: P38431
Secondary accession number(s): D6W450
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 175 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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