Transcriptional regulatory protein SAP30 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38429 (SAP30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein SAP30

Gene names

Name:	SAP30
Ordered Locus Names:	YMR263W
ORF Names:	YM8156.05

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Ref.5 Ref.6
Subunit structure	Component of the RPD3C(L) complex composed of at least ASH1, CTI6, DEP1, PHO23, RPD3, RXT2, RXT3, SAP30, SDS3, SIN3, UME1 and UME6.
Subcellular location	Nucleus Ref.8.
Miscellaneous	Present with 704 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the SAP30 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Chromatin regulator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to heat Inferred from mutant phenotype. Source: SGD negative regulation of chromatin silencing at rDNA Inferred from mutant phenotype Ref.6 Ref.11. Source: SGD negative regulation of chromatin silencing at silent mating-type cassette Inferred from mutant phenotype Ref.6 Ref.11. Source: SGD negative regulation of chromatin silencing at telomere Inferred from mutant phenotype Ref.6 Ref.11. Source: SGD negative regulation of transcription from RNA polymerase I promoter Inferred from mutant phenotype. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Rpd3L complex Inferred from direct assay Ref.11 Ref.10. Source: SGD Rpd3L-Expanded complex Inferred from direct assay. Source: SGD
Molecular function	histone deacetylase activity Inferred from physical interaction Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 201

201

Transcriptional regulatory protein SAP30

PRO_0000203345

Regions

Compositional bias

26 – 58

Asn-rich

Experimental info

Sequence conflict

C → S in AAA35222. Ref.1

Sequence conflict

N → S in AAA35222. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P38429 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 6C0D2734C50DD72E
Show »

FASTA

201

23,026

        10         20         30         40         50         60 
MARPVNTNAE TESRGRPTQG GGYASNNNGS CNNNNGSNNN NNNNNNNNNN SNNSNNNNGP 

        70         80         90        100        110        120 
TSSGRTNGKQ RLTAAQQQYI KNLIETHITD NHPDLRPKSH PMDFEEYTDA FLRRYKDHFQ 

       130        140        150        160        170        180 
LDVPDNLTLQ GYLLGSKLGA KTYSYKRNTQ GQHDKRIHKR DLANVVRRHF DEHSIKETDC 

       190        200 
IPQFIYKVKN QKKKFKMEFR G

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A large open reading frame in the yeast genome containing homology to the cif1 gene." Manning A.M., Rosenbloom C.L., Beaudet A.L. Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"SAP30, a novel protein conserved between human and yeast, is a component of a histone deacetylase complex." Zhang Y., Sun Z.-W., Iratni R., Erdjument-Bromage H., Tempst P., Hampsey M., Reinberg D. Mol. Cell 1:1021-1031(1998) [PubMed: 9651585] [Abstract] Cited for: FUNCTION.
[6]	"A general requirement for the Sin3-Rpd3 histone deacetylase complex in regulating silencing in Saccharomyces cerevisiae." Sun Z.-W., Hampsey M. Genetics 152:921-932(1999) [PubMed: 10388812] [Abstract] Cited for: FUNCTION.
[7]	"Opposite role of yeast ING family members in p53-dependent transcriptional activation." Nourani A., Howe L., Pray-Grant M.G., Workman J.L., Grant P.A., Cote J. J. Biol. Chem. 278:19171-19175(2003) [PubMed: 12672825] [Abstract] Cited for: IDENTIFICATION IN THE RPD3 COMPLEX, MASS SPECTROMETRY.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex." Carrozza M.J., Florens L., Swanson S.K., Shia W.-J., Anderson S., Yates J., Washburn M.P., Workman J.L. Biochim. Biophys. Acta 1731:77-87(2005) [PubMed: 16314178] [Abstract] Cited for: IDENTIFICATION IN THE RPD3C(L) COMPLEX, MASS SPECTROMETRY.
[11]	"Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex." Keogh M.-C., Kurdistani S.K., Morris S.A., Ahn S.H., Podolny V., Collins S.R., Schuldiner M., Chin K., Punna T., Thompson N.J., Boone C., Emili A., Weissman J.S., Hughes T.R., Strahl B.D., Grunstein M., Greenblatt J.F., Buratowski S., Krogan N.J. Cell 123:593-605(2005) [PubMed: 16286008] [Abstract] Cited for: IDENTIFICATION IN THE RPD3C(L) COMPLEX, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88172 Genomic DNA. Translation: AAA35222.1. Z49260 Genomic DNA. Translation: CAA89246.1. AY557972 Genomic DNA. Translation: AAS56298.1. BK006946 Genomic DNA. Translation: DAA10163.1.
PIR	S54475.
RefSeq	NP_013990.1. NM_001182770.1.
3D structure databases
ProteinModelPortal	P38429.
SMR	P38429. Positions 101-198.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3846N.
IntAct	P38429. 16 interactions.
MINT	MINT-520750.
STRING	P38429.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR263W; YMR263W; YMR263W.
GeneID	855305.
KEGG	sce:YMR263W.
NMPDR	fig\|4932.3.peg.5046.
Organism-specific databases
CYGD	YMR263w.
SGD	S000004876. SAP30.
Phylogenomic databases
eggNOG	fuNOG09973.
GeneTree	EFGT00050000000132.
HOGENOM	HBG397393.
OMA	HINSNGP.
OrthoDB	EOG4G7G81.
Gene expression databases
ArrayExpress	P38429.
Genevestigator	P38429.
GermOnline	YMR263W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	978979.

Entry information

Entry name

SAP30_YEAST

Accession

Primary (citable) accession number: P38429
Secondary accession number(s): D6W089, Q03501

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents