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Protein

Coupling of ubiquitin conjugation to ER degradation protein 1

Gene

CUE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Recruits the soluble ubiquitin-conjugating enzyme UBC7 to the cytoplasmic face of the endoplasmic reticulum membrane where it functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Targets the E2 conjugating enzyme UBC7 to the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains, and to the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). Has also a role in cold adaptation, perhaps through effects on sterol biosynthesis.11 Publications

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ubiquitin binding Source: SGD
  • ubiquitin-protein transferase activator activity Source: SGD

GO - Biological processi

  • establishment of protein localization to endoplasmic reticulum membrane Source: SGD
  • ubiquitin-dependent ERAD pathway Source: SGD

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32938-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Coupling of ubiquitin conjugation to ER degradation protein 1
Alternative name(s):
Kinetochore-defect suppressor 4
Gene namesi
Name:CUE1
Synonyms:KIS4
Ordered Locus Names:YMR264W
ORF Names:YM8156.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR264W
SGDiS000004877 CUE1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6Lumenal1 Publication6
Transmembranei7 – 23HelicalSequence analysisAdd BLAST17
Topological domaini24 – 203Cytoplasmic1 PublicationAdd BLAST180

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002033461 – 203Coupling of ubiquitin conjugation to ER degradation protein 1Add BLAST203

Proteomic databases

MaxQBiP38428
PaxDbiP38428
PRIDEiP38428

Interactioni

Subunit structurei

Forms a heterodimer with UBC7. Interacts with SSM4/DOA10 and UBX2/SEL1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SSM4P403182EBI-27580,EBI-18208

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi35442, 173 interactors
ComplexPortaliCPX-2948 CUE1-UBC7 ubiquitin-conjugating enzyme complex
DIPiDIP-4399N
IntActiP38428, 8 interactors
MINTiP38428
STRINGi4932.YMR264W

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi67 – 76Combined sources10
Helixi82 – 92Combined sources11
Helixi95 – 103Combined sources9
Turni112 – 114Combined sources3
Helixi151 – 154Combined sources4
Helixi168 – 170Combined sources3
Helixi173 – 192Combined sources20
Helixi196 – 202Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MYXNMR-A45-115[»]
4JQUX-ray1.81B151-203[»]
ProteinModelPortaliP38428
SMRiP38428
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 107CUEPROSITE-ProRule annotationAdd BLAST43

Sequence similaritiesi

Belongs to the CUE1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000248336
InParanoidiP38428
KOiK14022
OMAiWLIAPIP
OrthoDBiEOG092C5I2D

Family and domain databases

InterProiView protein in InterPro
IPR003892 CUE
PfamiView protein in Pfam
PF02845 CUE, 1 hit
SMARTiView protein in SMART
SM00546 CUE, 1 hit
PROSITEiView protein in PROSITE
PS51140 CUE, 1 hit

Sequencei

Sequence statusi: Complete.

P38428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSRLLITL ILVFGVIFLK KFFQSNQHPS AQRLSATGVN AHGRPQGSTQ
60 70 80 90 100
NALRRTGRVN GGHPVTTQMV ETVQNLAPNL HPEQIRYSLE NTGSVEETVE
110 120 130 140 150
RYLRGDEFSF PPGFEPSRAP MGANAAVDNN AAGGGEFNDP RKKNMICAEN
160 170 180 190 200
LLDKFHVDLN EDMSNLSFKD LDIEERKRLL VWQARKNLET KLQSDKDLQS

LLT
Length:203
Mass (Da):22,763
Last modified:November 1, 1997 - v2
Checksum:i3F795BD78DAC3D5B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48S → P in AAA35221 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49260 Genomic DNA Translation: CAA89247.1
AY557973 Genomic DNA Translation: AAS56299.1
M88172 Genomic DNA Translation: AAA35221.1
BK006946 Genomic DNA Translation: DAA10164.1
PIRiS54476
RefSeqiNP_013991.1, NM_001182771.1

Genome annotation databases

EnsemblFungiiYMR264W; YMR264W; YMR264W
GeneIDi855306
KEGGisce:YMR264W

Similar proteinsi

Entry informationi

Entry nameiCUE1_YEAST
AccessioniPrimary (citable) accession number: P38428
Secondary accession number(s): D6W090, Q03507
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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