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Protein

Coupling of ubiquitin conjugation to ER degradation protein 1

Gene

CUE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Recruits the soluble ubiquitin-conjugating enzyme UBC7 to the cytoplasmic face of the endoplasmic reticulum membrane where it functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Targets the E2 conjugating enzyme UBC7 to the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains, and to the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). Has also a role in cold adaptation, perhaps through effects on sterol biosynthesis.11 Publications

GO - Molecular functioni

  • ubiquitin binding Source: SGD
  • ubiquitin-protein transferase activator activity Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • establishment of protein localization to endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-32938-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Coupling of ubiquitin conjugation to ER degradation protein 1
Alternative name(s):
Kinetochore-defect suppressor 4
Gene namesi
Name:CUE1
Synonyms:KIS4
Ordered Locus Names:YMR264W
ORF Names:YM8156.06
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR264W.
SGDiS000004877. CUE1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6Lumenal1 Publication6
Transmembranei7 – 23HelicalSequence analysisAdd BLAST17
Topological domaini24 – 203Cytoplasmic1 PublicationAdd BLAST180

GO - Cellular componenti

  • Doa10p ubiquitin ligase complex Source: SGD
  • Hrd1p ubiquitin ligase ERAD-L complex Source: SGD
  • integral component of endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002033461 – 203Coupling of ubiquitin conjugation to ER degradation protein 1Add BLAST203

Proteomic databases

MaxQBiP38428.
PRIDEiP38428.

Interactioni

Subunit structurei

Forms a heterodimer with UBC7. Interacts with SSM4/DOA10 and UBX2/SEL1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SSM4P403182EBI-27580,EBI-18208

GO - Molecular functioni

  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi35442. 113 interactors.
DIPiDIP-4399N.
IntActiP38428. 8 interactors.
MINTiMINT-531548.

Structurei

Secondary structure

1203
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi67 – 76Combined sources10
Helixi82 – 92Combined sources11
Helixi95 – 103Combined sources9
Turni112 – 114Combined sources3
Helixi151 – 154Combined sources4
Helixi168 – 170Combined sources3
Helixi173 – 192Combined sources20
Helixi196 – 202Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MYXNMR-A45-115[»]
4JQUX-ray1.81B151-203[»]
ProteinModelPortaliP38428.
SMRiP38428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 107CUEPROSITE-ProRule annotationAdd BLAST43

Sequence similaritiesi

Belongs to the CUE1 family.Curated
Contains 1 CUE domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000248336.
InParanoidiP38428.
KOiK14022.
OMAiHPEQIRY.
OrthoDBiEOG092C5I2D.

Family and domain databases

InterProiIPR003892. CUE.
[Graphical view]
PfamiPF02845. CUE. 1 hit.
[Graphical view]
SMARTiSM00546. CUE. 1 hit.
[Graphical view]
PROSITEiPS51140. CUE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSRLLITL ILVFGVIFLK KFFQSNQHPS AQRLSATGVN AHGRPQGSTQ
60 70 80 90 100
NALRRTGRVN GGHPVTTQMV ETVQNLAPNL HPEQIRYSLE NTGSVEETVE
110 120 130 140 150
RYLRGDEFSF PPGFEPSRAP MGANAAVDNN AAGGGEFNDP RKKNMICAEN
160 170 180 190 200
LLDKFHVDLN EDMSNLSFKD LDIEERKRLL VWQARKNLET KLQSDKDLQS

LLT
Length:203
Mass (Da):22,763
Last modified:November 1, 1997 - v2
Checksum:i3F795BD78DAC3D5B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48S → P in AAA35221 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49260 Genomic DNA. Translation: CAA89247.1.
AY557973 Genomic DNA. Translation: AAS56299.1.
M88172 Genomic DNA. Translation: AAA35221.1.
BK006946 Genomic DNA. Translation: DAA10164.1.
PIRiS54476.
RefSeqiNP_013991.1. NM_001182771.1.

Genome annotation databases

EnsemblFungiiYMR264W; YMR264W; YMR264W.
GeneIDi855306.
KEGGisce:YMR264W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49260 Genomic DNA. Translation: CAA89247.1.
AY557973 Genomic DNA. Translation: AAS56299.1.
M88172 Genomic DNA. Translation: AAA35221.1.
BK006946 Genomic DNA. Translation: DAA10164.1.
PIRiS54476.
RefSeqiNP_013991.1. NM_001182771.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MYXNMR-A45-115[»]
4JQUX-ray1.81B151-203[»]
ProteinModelPortaliP38428.
SMRiP38428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35442. 113 interactors.
DIPiDIP-4399N.
IntActiP38428. 8 interactors.
MINTiMINT-531548.

Proteomic databases

MaxQBiP38428.
PRIDEiP38428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR264W; YMR264W; YMR264W.
GeneIDi855306.
KEGGisce:YMR264W.

Organism-specific databases

EuPathDBiFungiDB:YMR264W.
SGDiS000004877. CUE1.

Phylogenomic databases

HOGENOMiHOG000248336.
InParanoidiP38428.
KOiK14022.
OMAiHPEQIRY.
OrthoDBiEOG092C5I2D.

Enzyme and pathway databases

BioCyciYEAST:G3O-32938-MONOMER.

Miscellaneous databases

PROiP38428.

Family and domain databases

InterProiIPR003892. CUE.
[Graphical view]
PfamiPF02845. CUE. 1 hit.
[Graphical view]
SMARTiSM00546. CUE. 1 hit.
[Graphical view]
PROSITEiPS51140. CUE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCUE1_YEAST
AccessioniPrimary (citable) accession number: P38428
Secondary accession number(s): D6W090, Q03507
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 589 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.