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Protein

Lipoxygenase 7, chloroplastic

Gene

CM-LOX1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This lipoxygenase introduces molecular oxygen exclusively into the C-13 position of linoleic and linolenic acids.1 Publication

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi581Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi586Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi773Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi777Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi924Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-KW
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • response to other organism Source: Gramene
  • response to wounding Source: Gramene
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16718.
BRENDAi1.13.11.12. 4460.
ReactomeiR-OSA-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-OSA-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-OSA-2142700. Synthesis of Lipoxins (LX).
R-OSA-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-OSA-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
R-OSA-6798695. Neutrophil degranulation.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 7, chloroplastic (EC:1.13.11.12)
Gene namesi
Name:CM-LOX1
Synonyms:LOX2.1
Ordered Locus Names:Os08g0508800, LOC_Os08g39840
ORF Names:B1168A08.24
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 61ChloroplastSequence analysisAdd BLAST61
ChainiPRO_000001832762 – 924Lipoxygenase 7, chloroplasticAdd BLAST863

Proteomic databases

PaxDbiP38419.
PRIDEiP38419.

Expressioni

Inductioni

By fungal infection.

Gene expression databases

ExpressionAtlasiP38419. baseline and differential.
GenevisibleiP38419. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os08g39840.1.

Structurei

3D structure databases

ProteinModelPortaliP38419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 218PLATPROSITE-ProRule annotationAdd BLAST131
Domaini225 – 924LipoxygenasePROSITE-ProRule annotationAdd BLAST700

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIW1. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiP38419.
KOiK00454.
OMAiPANIQSR.
OrthoDBiEOG093601O5.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38419-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRPQLNPSS HTTTTSSSSS TQLFASSSCI ASLRRPSSSS SSVVAAARRT
60 70 80 90 100
RGQGSSRVVV VCASSSATAS RGDSSSDMAA AAAVRVKAVA TIKVTVGELI
110 120 130 140 150
NRSIDIRDLI GRSLSLELVS SELDAKTGKE KATVRSYAHN VDDDDHSVVT
160 170 180 190 200
YEADFDVPSG FGPIGAIIVT NELRQEMFLE DINLTASDGA GNSTVLPIRC
210 220 230 240 250
NSWVQPKSVG DEGTPSKRIF FANKTYLPGQ TPAGLRSYRK NDLQQKRGDG
260 270 280 290 300
TGEREADDRV YDYDVYNDLG NPDSNGDLAR PVLGGNKQFP YPRRCRTGRP
310 320 330 340 350
PSKKDPKSET RKGNVYVPRD EEFSPEKEDY FLRKTVGSVL QAAVPAAQSL
360 370 380 390 400
LLDKLKWNLP FPSFFVIDKL FEDGVELPGV DKLNFLESVV PRLLEHLRDT
410 420 430 440 450
PAEKILRFET PANIQKDKFA WLRDEEFARE TLAGINPYAI ELVREFPLKS
460 470 480 490 500
KLDPAVYGPA ESAITADLLE EQMRRVMTVE EAISQKRLFM LDFHDLFLPY
510 520 530 540 550
VHKIRSLDHT TMYGSRTVFF LTDDGTLQLL AIELTRPASP SQPQWRQVFT
560 570 580 590 600
PSTDATMSWL WRMAKAHVRA HDAGHHELIT HWLRTHCAVE PYIIAANRQL
610 620 630 640 650
SEMHPIYQLL RPHFRYTMRI NARARSALIS AGGIIERSFS PQKYSMELSS
660 670 680 690 700
VAYDKLWRFD TEALPADLVR RGMAEEDPTA EHGLKLAIED YPFANDGLLI
710 720 730 740 750
WDAIKTWVQA YVARFYPDAD SVAGDEELQA FWTEVRTKGH GDKKDAPWWP
760 770 780 790 800
KLDSPESLAH TLTTIVWVAA AHHAAVNFGQ YDFGGYFPNR PSIARTVMPV
810 820 830 840 850
EEPVDGAAME RFLDNPDQAL RECFPSQVQA TVVMAVLDVL SSHSTDEEYL
860 870 880 890 900
GGEQTRPWNS DAAVQAAYDG FAARLKEIEG VIDGRNKDRK LKNRCGAGIL
910 920
PYQLMKPFSD SGVTGMGIPN STSI
Length:924
Mass (Da):102,819
Last modified:August 30, 2005 - v2
Checksum:iE2142F9775751FEB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143D → E in AAD39093 (PubMed:7508918).Curated1
Sequence conflicti342 – 343AA → P in AAD39093 (PubMed:7508918).Curated2
Sequence conflicti350 – 352LLL → CSS in BAA03102 (PubMed:7508918).Curated3
Sequence conflicti450S → T in AAD39093 (PubMed:7508918).Curated1
Sequence conflicti484S → N in BAA03102 (PubMed:7508918).Curated1
Sequence conflicti540P → L in BAA03102 (PubMed:7508918).Curated1
Sequence conflicti625 – 628RSAL → ARV in BAA03102 (PubMed:7508918).Curated4
Sequence conflicti682H → Q in BAA03102 (PubMed:7508918).Curated1
Sequence conflicti764T → N in BAA03102 (PubMed:7508918).Curated1
Sequence conflicti819A → R in AAD39093 (PubMed:7508918).Curated1
Sequence conflicti858 – 864WNSDAAV → GTATRRL in BAA03102 (PubMed:7508918).Curated7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14000 mRNA. Translation: BAA03102.1.
AF095895 mRNA. Translation: AAD39093.1.
AP005816 Genomic DNA. Translation: BAD10665.1.
AP014964 Genomic DNA. Translation: BAT06178.1.
RefSeqiXP_015650717.1. XM_015795231.1.
UniGeneiOs.4416.

Genome annotation databases

EnsemblPlantsiOS08T0508800-01; OS08T0508800-01; OS08G0508800.
GeneIDi4345993.
GrameneiOS08T0508800-01; OS08T0508800-01; OS08G0508800.
KEGGiosa:4345993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14000 mRNA. Translation: BAA03102.1.
AF095895 mRNA. Translation: AAD39093.1.
AP005816 Genomic DNA. Translation: BAD10665.1.
AP014964 Genomic DNA. Translation: BAT06178.1.
RefSeqiXP_015650717.1. XM_015795231.1.
UniGeneiOs.4416.

3D structure databases

ProteinModelPortaliP38419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os08g39840.1.

Proteomic databases

PaxDbiP38419.
PRIDEiP38419.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0508800-01; OS08T0508800-01; OS08G0508800.
GeneIDi4345993.
GrameneiOS08T0508800-01; OS08T0508800-01; OS08G0508800.
KEGGiosa:4345993.

Phylogenomic databases

eggNOGiENOG410IIW1. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiP38419.
KOiK00454.
OMAiPANIQSR.
OrthoDBiEOG093601O5.

Enzyme and pathway databases

UniPathwayiUPA00382.
BioCyciMetaCyc:MONOMER-16718.
BRENDAi1.13.11.12. 4460.
ReactomeiR-OSA-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-OSA-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-OSA-2142700. Synthesis of Lipoxins (LX).
R-OSA-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-OSA-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
R-OSA-6798695. Neutrophil degranulation.

Gene expression databases

ExpressionAtlasiP38419. baseline and differential.
GenevisibleiP38419. OS.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOXC1_ORYSJ
AccessioniPrimary (citable) accession number: P38419
Secondary accession number(s): Q6YVT0, Q9XHM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: August 30, 2005
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.