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Protein

Lipoxygenase 2, chloroplastic

Gene

LOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

13S-lipoxygenase that can use linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Required for the wound-induced synthesis of jasmonic acid (JA) in leaves.2 Publications

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi554 – 5541Iron; catalyticPROSITE-ProRule annotation
Metal bindingi559 – 5591Iron; catalyticPROSITE-ProRule annotation
Metal bindingi746 – 7461Iron; catalyticPROSITE-ProRule annotation
Metal bindingi750 – 7501Iron; catalyticPROSITE-ProRule annotation
Metal bindingi896 – 8961Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

  • linoleate 13S-lipoxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • jasmonic acid biosynthetic process Source: TAIR
  • lipid oxidation Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • response to bacterium Source: UniProtKB
  • response to fungus Source: TAIR
  • response to herbivore Source: UniProtKB
  • response to jasmonic acid Source: TAIR
  • response to wounding Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT3G45140-MONOMER.
BRENDAi1.13.11.12. 399.
ReactomeiR-ATH-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-ATH-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-ATH-2142700. Synthesis of Lipoxins (LX).
R-ATH-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-ATH-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 2, chloroplastic (EC:1.13.11.12)
Short name:
AtLOX2
Gene namesi
Name:LOX2
Ordered Locus Names:At3g45140
ORF Names:T14D3.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G45140.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: UniProtKB
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5656ChloroplastSequence analysisAdd
BLAST
Chaini57 – 896840Lipoxygenase 2, chloroplasticPRO_0000018326Add
BLAST

Proteomic databases

PaxDbiP38418.
PRIDEiP38418.

2D gel databases

SWISS-2DPAGEP38418.

PTM databases

iPTMnetiP38418.

Expressioni

Tissue specificityi

In leaves and inflorescences but not abundant in seeds, roots and stems.1 Publication

Developmental stagei

Expression is sharply reduced in leaves during leaf senescence.1 Publication

Inductioni

By methyl jasmonate (MeJA) and wounding, probably through nitric oxide-mediated (NO) induction. Slightly locally induced upon herbivors infestation such as aphids (Myzus persicae and Brevicoryne brassicae), or caterpillar (Spodoptera exigua). Induced by leaf-volatiles generated by herbivors-mediated wounding such as (E)-2-hexenal, (Z)-3-hexenal, (Z)-3-hexenol or allo-ocimene (2,6-dimethyl-2,4,6-octatriene). Increased levels by bacterial pathogens (e.g. P.viridiflava and P.syringae pv. tomato). Repressed by WRKY62.9 Publications

Gene expression databases

GenevisibleiP38418. AT.

Interactioni

Subunit structurei

Interacts with EIF4E2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EIF(ISO)4EO046637EBI-1770437,EBI-1770425

Protein-protein interaction databases

BioGridi8970. 2 interactions.
IntActiP38418. 4 interactions.
STRINGi3702.AT3G45140.1.

Structurei

3D structure databases

ProteinModelPortaliP38418.
SMRiP38418. Positions 132-896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini79 – 199121PLATPROSITE-ProRule annotationAdd
BLAST
Domaini202 – 896695LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni175 – 23258EIF4E2 bindingAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIW1. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiP38418.
KOiK00454.
OMAiPANIQSR.
OrthoDBiEOG093601O5.
PhylomeDBiP38418.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYCRESLSSL QTLNVAKSLS SLFPKQSALI NPISAGRRNN LPRPNLRRRC
60 70 80 90 100
KVTASRANIE QEGNTVKEPI QNIKVKGYIT AQEEFLEGIT WSRGLDDIAD
110 120 130 140 150
IRGRSLLVEL ISAKTDQRIT VEDYAQRVWA EAPDEKYECE FEMPEDFGPV
160 170 180 190 200
GAIKIQNQYH RQLFLKGVEL KLPGGSITFT CESWVAPKSV DPTKRIFFSD
210 220 230 240 250
KSYLPSQTPE PLKKYRKEEL ETLQGKNREE VGEFTKFERI YDYDVYNDVG
260 270 280 290 300
DPDNDPELAR PVIGGLTHPY PRRCKTGRKP CETDPSSEQR YGGEFYVPRD
310 320 330 340 350
EEFSTAKGTS FTGKAVLAAL PSIFPQIESV LLSPQEPFPH FKAIQNLFEE
360 370 380 390 400
GIQLPKDAGL LPLLPRIIKA LGEAQDDILQ FDAPVLINRD RFSWLRDDEF
410 420 430 440 450
ARQTLAGLNP YSIQLVEEWP LISKLDPAVY GDPTSLITWE IVEREVKGNM
460 470 480 490 500
TVDEALKNKR LFVLDYHDLL LPYVNKVREL NNTTLYASRT LFFLSDDSTL
510 520 530 540 550
RPVAIELTCP PNINKPQWKQ VFTPGYDATS CWLWNLAKTH AISHDAGYHQ
560 570 580 590 600
LISHWLRTHA CTEPYIIAAN RQLSAMHPIY RLLHPHFRYT MEINARARQS
610 620 630 640 650
LVNGGGIIET CFWPGKYALE LSSAVYGKLW RFDQEGLPAD LIKRGLAEED
660 670 680 690 700
KTAEHGVRLT IPDYPFANDG LILWDAIKEW VTDYVKHYYP DEELITSDEE
710 720 730 740 750
LQGWWSEVRN IGHGDKKDEP WWPVLKTQDD LIGVVTTIAW VTSGHHAAVN
760 770 780 790 800
FGQYGYGGYF PNRPTTTRIR MPTEDPTDEA LKEFYESPEK VLLKTYPSQK
810 820 830 840 850
QATLVMVTLD LLSTHSPDEE YIGEQQEASW ANEPVINAAF ERFKGKLQYL
860 870 880 890
EGVIDERNVN ITLKNRAGAG VVKYELLKPT SEHGVTGMGV PYSISI
Length:896
Mass (Da):102,046
Last modified:October 1, 1994 - v1
Checksum:iF32822205C8F9F22
GO

Sequence cautioni

The sequence CAB72152 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti119 – 1257ITVEDYA → EYTFFFL in BAC43666 (PubMed:11910074).Curated
Sequence conflicti613 – 6131W → C in AAL32689 (PubMed:14593172).Curated
Sequence conflicti646 – 6461L → V in BAF01939 (Ref. 6) Curated
Sequence conflicti731 – 7311L → F in BAF01939 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23968 mRNA. Translation: AAA32749.1.
AL138649 Genomic DNA. Translation: CAB72152.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE77997.1.
AY062611 mRNA. Translation: AAL32689.1.
AK119093 mRNA. Translation: BAC43666.1.
AK222075 mRNA. Translation: BAD94917.1.
AK230124 mRNA. Translation: BAF01939.1.
PIRiJQ2391.
T47454.
RefSeqiNP_566875.1. NM_114383.2.
UniGeneiAt.22079.
At.75027.

Genome annotation databases

EnsemblPlantsiAT3G45140.1; AT3G45140.1; AT3G45140.
GeneIDi823650.
GrameneiAT3G45140.1; AT3G45140.1; AT3G45140.
KEGGiath:AT3G45140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23968 mRNA. Translation: AAA32749.1.
AL138649 Genomic DNA. Translation: CAB72152.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE77997.1.
AY062611 mRNA. Translation: AAL32689.1.
AK119093 mRNA. Translation: BAC43666.1.
AK222075 mRNA. Translation: BAD94917.1.
AK230124 mRNA. Translation: BAF01939.1.
PIRiJQ2391.
T47454.
RefSeqiNP_566875.1. NM_114383.2.
UniGeneiAt.22079.
At.75027.

3D structure databases

ProteinModelPortaliP38418.
SMRiP38418. Positions 132-896.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi8970. 2 interactions.
IntActiP38418. 4 interactions.
STRINGi3702.AT3G45140.1.

PTM databases

iPTMnetiP38418.

2D gel databases

SWISS-2DPAGEP38418.

Proteomic databases

PaxDbiP38418.
PRIDEiP38418.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G45140.1; AT3G45140.1; AT3G45140.
GeneIDi823650.
GrameneiAT3G45140.1; AT3G45140.1; AT3G45140.
KEGGiath:AT3G45140.

Organism-specific databases

TAIRiAT3G45140.

Phylogenomic databases

eggNOGiENOG410IIW1. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiP38418.
KOiK00454.
OMAiPANIQSR.
OrthoDBiEOG093601O5.
PhylomeDBiP38418.

Enzyme and pathway databases

UniPathwayiUPA00382.
BioCyciMetaCyc:AT3G45140-MONOMER.
BRENDAi1.13.11.12. 399.
ReactomeiR-ATH-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-ATH-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-ATH-2142700. Synthesis of Lipoxins (LX).
R-ATH-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-ATH-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.

Miscellaneous databases

PROiP38418.

Gene expression databases

GenevisibleiP38418. AT.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOX2_ARATH
AccessioniPrimary (citable) accession number: P38418
Secondary accession number(s): Q0WLR8
, Q56WG6, Q8GW45, Q8W4E4, Q9M1U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 7, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.