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Protein

Lipoxygenase 2, chloroplastic

Gene

LOX2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

13S-lipoxygenase that can use linolenic acid as substrates. Plant lipoxygenases may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. Required for the wound-induced synthesis of jasmonic acid (JA) in leaves.2 Publications

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi554Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi559Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi746Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi750Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi896Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • linoleate 13S-lipoxygenase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • green leaf volatile biosynthetic process Source: TAIR
  • jasmonic acid biosynthetic process Source: TAIR
  • lipid oxidation Source: TAIR
  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • response to bacterium Source: UniProtKB
  • response to fungus Source: TAIR
  • response to herbivore Source: UniProtKB
  • response to jasmonic acid Source: TAIR
  • response to wounding Source: UniProtKB

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G45140-MONOMER
MetaCyc:AT3G45140-MONOMER
BRENDAi1.13.11.12 399
ReactomeiR-ATH-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-ATH-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
R-ATH-2142700 Synthesis of Lipoxins (LX)
R-ATH-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-ATH-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-ATH-6798695 Neutrophil degranulation
UniPathwayiUPA00382

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 2, chloroplastic (EC:1.13.11.12)
Short name:
AtLOX2
Gene namesi
Name:LOX2
Ordered Locus Names:At3g45140
ORF Names:T14D3.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G45140
TAIRilocus:2096915 AT3G45140

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 56ChloroplastSequence analysisAdd BLAST56
ChainiPRO_000001832657 – 896Lipoxygenase 2, chloroplasticAdd BLAST840

Proteomic databases

PaxDbiP38418
PRIDEiP38418

2D gel databases

SWISS-2DPAGEiP38418

PTM databases

iPTMnetiP38418

Expressioni

Tissue specificityi

In leaves and inflorescences but not abundant in seeds, roots and stems.1 Publication

Developmental stagei

Expression is sharply reduced in leaves during leaf senescence.1 Publication

Inductioni

By methyl jasmonate (MeJA) and wounding, probably through nitric oxide-mediated (NO) induction. Slightly locally induced upon herbivors infestation such as aphids (Myzus persicae and Brevicoryne brassicae), or caterpillar (Spodoptera exigua). Induced by leaf-volatiles generated by herbivors-mediated wounding such as (E)-2-hexenal, (Z)-3-hexenal, (Z)-3-hexenol or allo-ocimene (2,6-dimethyl-2,4,6-octatriene). Increased levels by bacterial pathogens (e.g. P.viridiflava and P.syringae pv. tomato). Repressed by WRKY62.9 Publications

Gene expression databases

ExpressionAtlasiP38418 baseline and differential
GenevisibleiP38418 AT

Interactioni

Subunit structurei

Interacts with EIF4E2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EIF(ISO)4EO046637EBI-1770437,EBI-1770425

Protein-protein interaction databases

BioGridi8970, 2 interactors
IntActiP38418, 4 interactors
STRINGi3702.AT3G45140.1

Structurei

3D structure databases

ProteinModelPortaliP38418
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 199PLATPROSITE-ProRule annotationAdd BLAST121
Domaini202 – 896LipoxygenasePROSITE-ProRule annotationAdd BLAST695

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni175 – 232EIF4E2 bindingAdd BLAST58

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIW1 Eukaryota
ENOG410YN4N LUCA
HOGENOMiHOG000230469
InParanoidiP38418
KOiK00454
OMAiWITESVK
OrthoDBiEOG093601O5
PhylomeDBiP38418

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38418-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYCRESLSSL QTLNVAKSLS SLFPKQSALI NPISAGRRNN LPRPNLRRRC
60 70 80 90 100
KVTASRANIE QEGNTVKEPI QNIKVKGYIT AQEEFLEGIT WSRGLDDIAD
110 120 130 140 150
IRGRSLLVEL ISAKTDQRIT VEDYAQRVWA EAPDEKYECE FEMPEDFGPV
160 170 180 190 200
GAIKIQNQYH RQLFLKGVEL KLPGGSITFT CESWVAPKSV DPTKRIFFSD
210 220 230 240 250
KSYLPSQTPE PLKKYRKEEL ETLQGKNREE VGEFTKFERI YDYDVYNDVG
260 270 280 290 300
DPDNDPELAR PVIGGLTHPY PRRCKTGRKP CETDPSSEQR YGGEFYVPRD
310 320 330 340 350
EEFSTAKGTS FTGKAVLAAL PSIFPQIESV LLSPQEPFPH FKAIQNLFEE
360 370 380 390 400
GIQLPKDAGL LPLLPRIIKA LGEAQDDILQ FDAPVLINRD RFSWLRDDEF
410 420 430 440 450
ARQTLAGLNP YSIQLVEEWP LISKLDPAVY GDPTSLITWE IVEREVKGNM
460 470 480 490 500
TVDEALKNKR LFVLDYHDLL LPYVNKVREL NNTTLYASRT LFFLSDDSTL
510 520 530 540 550
RPVAIELTCP PNINKPQWKQ VFTPGYDATS CWLWNLAKTH AISHDAGYHQ
560 570 580 590 600
LISHWLRTHA CTEPYIIAAN RQLSAMHPIY RLLHPHFRYT MEINARARQS
610 620 630 640 650
LVNGGGIIET CFWPGKYALE LSSAVYGKLW RFDQEGLPAD LIKRGLAEED
660 670 680 690 700
KTAEHGVRLT IPDYPFANDG LILWDAIKEW VTDYVKHYYP DEELITSDEE
710 720 730 740 750
LQGWWSEVRN IGHGDKKDEP WWPVLKTQDD LIGVVTTIAW VTSGHHAAVN
760 770 780 790 800
FGQYGYGGYF PNRPTTTRIR MPTEDPTDEA LKEFYESPEK VLLKTYPSQK
810 820 830 840 850
QATLVMVTLD LLSTHSPDEE YIGEQQEASW ANEPVINAAF ERFKGKLQYL
860 870 880 890
EGVIDERNVN ITLKNRAGAG VVKYELLKPT SEHGVTGMGV PYSISI
Length:896
Mass (Da):102,046
Last modified:October 1, 1994 - v1
Checksum:iF32822205C8F9F22
GO

Sequence cautioni

The sequence CAB72152 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119 – 125ITVEDYA → EYTFFFL in BAC43666 (PubMed:11910074).Curated7
Sequence conflicti613W → C in AAL32689 (PubMed:14593172).Curated1
Sequence conflicti646L → V in BAF01939 (Ref. 6) Curated1
Sequence conflicti731L → F in BAF01939 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23968 mRNA Translation: AAA32749.1
AL138649 Genomic DNA Translation: CAB72152.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE77997.1
AY062611 mRNA Translation: AAL32689.1
AK119093 mRNA Translation: BAC43666.1
AK222075 mRNA Translation: BAD94917.1
AK230124 mRNA Translation: BAF01939.1
PIRiJQ2391
T47454
RefSeqiNP_566875.1, NM_114383.3
UniGeneiAt.22079
At.75027

Genome annotation databases

EnsemblPlantsiAT3G45140.1; AT3G45140.1; AT3G45140
GeneIDi823650
GrameneiAT3G45140.1; AT3G45140.1; AT3G45140
KEGGiath:AT3G45140

Similar proteinsi

Entry informationi

Entry nameiLOX2_ARATH
AccessioniPrimary (citable) accession number: P38418
Secondary accession number(s): Q0WLR8
, Q56WG6, Q8GW45, Q8W4E4, Q9M1U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 25, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health