Reviewed, UniProtKB/Swiss-Prot P38417 (LOX4_SOYBN)

Names and origin

Protein names

Recommended name:
    Lipoxygenase-4
      Short name=L-4
    EC=1.13.11.12
Alternative name(s):
    VSP94

Gene names

Name:	LOX1.5
Synonyms:	LOX4

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
Catalytic activity	Linoleate + O(2) = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Found in maturing and developing seeds. In young seedlings it is found in cotyledons, hypocotyls, roots and primary leaves.
Developmental stage	Developmentally regulated. Expression is high 3 to 5 days after germination and returns to basal level by day 9.
Induction	By pod removal and methyl jasmonate.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 853	853	Lipoxygenase-4		PRO_0000220720
Regions
	Domain	34 – 159	126	PLAT
	Domain	162 – 853	692	Lipoxygenase
Sites
	Metal binding	513	1	Iron; catalytic By similarity
	Metal binding	518	1	Iron; catalytic By similarity
	Metal binding	704	1	Iron; catalytic By similarity
	Metal binding	708	1	Iron; catalytic By similarity
	Metal binding	853	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38417-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 533175EE1CFB34FC	FASTA	853	96,535
	10 20 30 40 50 60 MFPFGQKGQK IKGTMVVMQK NVLDINSITS VGGIVDQGLG FIGSAVDALT FAATKISIQL 70 80 90 100 110 120 ISATKADGGK GKIGKSTNLR GKITLPTLGA GEQAYDVNFE WDSDFGIPGA FYIKNFMQNE 130 140 150 160 170 180 FYLKSLILED IPNHGTIHFV CNSWVYNSKN YKTDRIFFAN NTYLPSETPA PLLKYREEEL 190 200 210 220 230 240 KNVRGDGTGE RKEWDRIYDY DVYNDLGNPD SGDKYARPVL GGSALPYPRR ERTGRGKTRK 250 260 270 280 290 300 DPNSEKPSDF VYLPRDEAFG HLKSSDFLAY GIKSVSQDVL PVLTDAFDGN ILSLEFDNFA 310 320 330 340 350 360 EVHKLYEGGV TLPTNFLSKI APIPVIKEIF RTDGEQFLKY PPPKVMQVDK SAWMTDEEFA 370 380 390 400 410 420 RETIAGLNPN VIKIIEEFPL SSKLDTQAYG DHTCIIAKEH LEPNLGGLTV EQAIQNKKLF 430 440 450 460 470 480 ILDHHDYLIP YLRKINANTT KTYATRTIFF LKDDGTLTPL AIELSKPHPQ GEEYGPVSEV 490 500 510 520 530 540 YVPASEGVEA YIWLLAKAYV VVNDACYHQI ISHWLSTHAI VEPFVIATNR QLSVVHPIYK 550 560 570 580 590 600 LLFPHYRDTM NINSLARKAL VNADGIIEKT FLWGRYSMEM SAVIYKDWVF TDQALPNDLV 610 620 630 640 650 660 KRGVAVKDPS APHGVRLLIE DYPYASDGLE IWDAIKSWVQ EYVSFYYKSD EELQKDPELQ 670 680 690 700 710 720 AWWKELVEVG HGDLKDKPWW QKMQTREELV EASAILIWIA SALHAAVNFG QYPYGGLILN 730 740 750 760 770 780 RPTISRRFMP EKGSPEYDAL AKNPEKEFLK TITGKKETLI DLTVIEILSR HASDEFYLGQ 790 800 810 820 830 840 RDGGDYWTSD AGPLEAFKRF GKKLEEIEKK LIEKNKDETL RNRYGPAKMP YTLLYPSSEE 850 GLTFRGIPNS ISI

References

[1]	"Soybean lipoxygenase L-4, a major component of the 94-kilodalton storage protein in vegetative tissues: expression and accumulation in leaves induced by pod removal and by methyl jasmonate." Kato T., Shirano Y., Iwamoto H., Shibata D. Plant Cell Physiol. 34:1063-1072(1993) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Enrei.
[2]	"Appearance of new lipoxygenases in soybean cotyledons after germination and evidence for expression of a major new lipoxygenase gene." Kato T., Ohta H., Tanaka K., Shibata D. Plant Physiol. 98:324-330(1992) [PubMed: 16668631] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Tissue: Cotyledon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13999 Genomic DNA. Translation: BAA03101.1.
PIR	T07662.
3D structure databases
HSSP	HSSP built from PDB template 1NO3 based on UniProtKB P09186.
ModBase	Search...
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX4_SOYBN

Accession

Primary (citable) accession number: P38417

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)