Reviewed, UniProtKB/Swiss-Prot P38416 (LOXB_SOLLC)

Names and origin

Protein names

Recommended name:
    Lipoxygenase B
    EC=1.13.11.12

Gene names

Name:	LOX1.2
Synonyms:	LOXB

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
Catalytic activity	Linoleate + O2 = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Fruit specific.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	Lipoxygenase B		PRO_0000220706
Regions
	Domain	34 – 158	125	PLAT
	Domain	161 – 859	699	Lipoxygenase
Sites
	Metal binding	521	1	Iron; catalytic By similarity
	Metal binding	526	1	Iron; catalytic By similarity
	Metal binding	711	1	Iron; catalytic By similarity
	Metal binding	859	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38416-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: DAD79B10AE627434	FASTA	859	97,122
	10 20 30 40 50 60 MSLGGIVDAI LGKDDRPKVK GRVILMKKNV LDFINIGASV VDGISDLLGQ KVSIQLISGS 70 80 90 100 110 120 VNYDGLEGKL SNPAYLESWL TDITPITAGE STFSVTFDWD RDEFGVPGAF IIKNLHLNEF 130 140 150 160 170 180 FLKSLTLEDV PNYGKIHFVC NSWVYPAFRY KSDRIFFANQ AYLPSETPQP LRKYRENELV 190 200 210 220 230 240 ALRGDGTGKL EEWDRVYDYA CYNDLGEPDK GEEYARPILG GSSEYPYPRR GRTGREPTKA 250 260 270 280 290 300 DPNCESRNPL PMSLDIYVPR DERFGHVKKS DFLTSSLKSS LQTLLPAFKA LCDNTPNEFN 310 320 330 340 350 360 SFADVLNLYE GGIKLPEGPW LKAITDNISS EILKDILQTD GQGLLKYPTP QVIQGDKTAW 370 380 390 400 410 420 RTDEEFGREM LAGSNPVLIS RLQEFPPKSK LDPTIYGNQN STITTEHVQD KLNGLTVNEA 430 440 450 460 470 480 IKSNRLFILN HHDIVMPLLR KINMSANTKA YASRTLLFLQ DDRTLKPLAI ELSLPHPDGD 490 500 510 520 530 540 QFGTVSKVYT PADQGVEGSI WQFAKAYVAV NDMGIHQLIS HWLNTHAVIE PFVVATNRHL 550 560 570 580 590 600 SVLHPIHKLL HPHFRNTMNI NALARETLTY DGGFETSLFP AKYSMEMSAA AYKDWVFPEQ 610 620 630 640 650 660 ALPADLLKRG VAVEDLSSPH GIRLLILDYP YAVDGLEIWA AIKSWVTEYC KFYYKSDETV 670 680 690 700 710 720 EKDTELQAWW KELREEGHGD KKDEAWWPKL QTRQELRDCC TIIIWIASAL HAALHFGLYS 730 740 750 760 770 780 YAGYLPNRPT LSCNLMPEPG SVEYEELKTN PDKVFLKTFV PQLQSLLEIS IFEVSSRHAS 790 800 810 820 830 840 DEVYLGQRDS IEWTKDKEPL VAFERFGKML SDIENRIMIM NSHKSWKNRS GPVNVPYTLL 850 FPTSEEGLTG KGIPNSVSI

References

[1]

"The cloning of two tomato lipoxygenase genes and their differential expression during fruit ripening." Ferrie B.J., Beaudoin N., Burkhart W., Bowsher C.G., Rothstein S.J. Plant Physiol. 106:109-118(1994) [PubMed: 7972514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Caruso. Tissue: Pericarp.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U09025 mRNA. Translation: AAA53183.1.
PIR	T06339.
3D structure databases
HSSP	HSSP built from PDB template 1NO3 based on UniProtKB P09186.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.12. 281054.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOXB_SOLLC

Accession

Primary (citable) accession number: P38416

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)