Reviewed, UniProtKB/Swiss-Prot P38414 (LOX1_LENCU)

Names and origin

Protein names

Recommended name:
    Lipoxygenase
    EC=1.13.11.12

Gene names

Name:

LOX1.1

Organism

Lens culinaris (Lentil) (Cicer lens)

Taxonomic identifier

3864 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Lens

Protein attributes

Sequence length	866 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
Catalytic activity	Linoleate + O(2) = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit. Iron is tightly bound By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 866	866	Lipoxygenase		PRO_0000220704
Regions
	Domain	42 – 171	130	PLAT
	Domain	174 – 866	693	Lipoxygenase
Sites
	Metal binding	526	1	Iron; catalytic By similarity
	Metal binding	531	1	Iron; catalytic By similarity
	Metal binding	717	1	Iron; catalytic By similarity
	Metal binding	721	1	Iron; catalytic By similarity
	Metal binding	866	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38414-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: E3E5D00E84E3C89C	FASTA	866	96,639
	10 20 30 40 50 60 MASLLFGRGQ KLKGTVILMQ KNVLDINALT AAQSPSGIIG GAFGVVGSIA GSIIDTATAF 70 80 90 100 110 120 LGRSVRLRLI SATVADASGK GKVSKEAFLE GLLTSIPTLG DKQSAFSVHF EWDSNMGTPG 130 140 150 160 170 180 AFYIENFMQG GEFFLVSLTL DDVPNVGSIK FACNSWIYND KKYQSDRIFF ANKTYLPSAT 190 200 210 220 230 240 PAPLVSYRQE ELKTLRGDGT GERQEWDRIY DYDVYNDLGA PDQKATLGRP VLGGSSTLPY 250 260 270 280 290 300 PRRGRTGRKK TVKEPQSESR SDTVYLPRDE AFGHVKSSDF LVYILKSASQ NIVPQLRSVV 310 320 330 340 350 360 TLQLNNPEFN TFEDVRSLYD GGIKLPTDVL SKISPIPLFS ELFRSDGEAA LKFPPPKVIQ 370 380 390 400 410 420 VDHSAWMTDE EFAREMIAGV NPHIIKEVLS FPIKSKLDSQ LYGDNTSKIT KEHLEPNLGG 430 440 450 460 470 480 VTVEGAIQTN RLFTPDHHDA LFPYLRKINA TATKAYATRT VLFLQDNGTL KPLAIELSTP 490 500 510 520 530 540 HPDGDSFGPV SKVYLPASEG VEASIWLLAK AFVVVNDSCY HQLVSHWLNT HAVVEPFIIA 550 560 570 580 590 600 TNRHLSVVHP IHKLLLPHYR DTMNINALAR NVLVNAEGII ESTFLWGNYA MEMSAVVYKD 610 620 630 640 650 660 WVFPDQGLPN DLIKRGVAVK DPSSPHGVRL LIEDYPYASD GLEIWAAIKS WVEEYVNFYY 670 680 690 700 710 720 KSDAAIAQDA ELQAFWKELV EVGHGDLKSA TWWFKMQNRK ELIEACSILI WIASALHAAV 730 740 750 760 770 780 NFGQYPYGGY ILNRPTKSRK FMPEKGTPEY DDLAKNYEKA YLRTITPKND TLTDLTIIEV 790 800 810 820 830 840 LSRHASDEQY LGERIEGDDW TTDSVPKEAF KRFGKKLAEI EEKLTQRNND ESLRNRYGPV 850 860 KMPYTLLYPS SEEGLTCRGI PNSISI

References

[1]

"The primary structure of a lipoxygenase from the shoots of etiolated lentil seedlings derived from its cDNA." Hilbers M.P., Rossi A., Finazzi-Agro A., Veldink G.A., Vliegenthart J.F.G. Biochim. Biophys. Acta 1211:239-242(1994) [PubMed: 8117753] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seedling shoot.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X71344 mRNA. Translation: CAA50483.1.
3D structure databases
HSSP	HSSP built from PDB template 2SBL based on UniProtKB P08170.
ModBase	Search...
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX1_LENCU

Accession

Primary (citable) accession number: P38414

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)