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Protein

Guanine nucleotide-binding protein G(olf) subunit alpha

Gene

Gnal

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561MagnesiumBy similarity
Metal bindingi191 – 1911MagnesiumBy similarity
Binding sitei353 – 3531GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 568GTPBy similarity
Nucleotide bindingi185 – 1917GTPBy similarity
Nucleotide bindingi210 – 2145GTPBy similarity
Nucleotide bindingi279 – 2824GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • signal transducer activity Source: UniProtKB-KW
  • toxin receptor binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(olf) subunit alpha
Alternative name(s):
Adenylate cyclase-stimulating G alpha protein, olfactory type
Gene namesi
Name:Gnal
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2715. Gnal.

Subcellular locationi

GO - Cellular componenti

  • heterotrimeric G-protein complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Guanine nucleotide-binding protein G(olf) subunit alphaPRO_0000203734Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-palmitoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity
Modified residuei178 – 1781PhosphothreonineBy similarity
Cross-linki287 – 287Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP38406.
PRIDEiP38406.

PTM databases

PhosphoSiteiP38406.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

GO - Molecular functioni

  • toxin receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025172.

Structurei

3D structure databases

ProteinModelPortaliP38406.
SMRiP38406. Positions 39-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(s) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0099. Eukaryota.
ENOG410XPC4. LUCA.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiP38406.
KOiK04633.
PhylomeDBiP38406.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P38406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCLGNSSKT AEDQGVDEKE RREANKKIEK QLQKERLAYK ATHRLLLLGA
60 70 80 90 100
GESGKSTIVK QMRILHVNGS NPEEKKQKIL DIRKNVKDAI VTIVSAMSTI
110 120 130 140 150
IPPVPLANPE NQFRSDYIKS IAPITDFEYS QEFFDHVKKL WDDEGVKACF
160 170 180 190 200
ERSNEYQLID CAQYFLERID SVSLVDYTPT DQDLLRCRVL TSGIFETRFQ
210 220 230 240 250
VDKVNFHMFD VGGQRDERRK WIQCFNDVTA IIYVAACSSY NMVIREDNNT
260 270 280 290 300
NRLRESLDLF ESIWNNRWLR TISIILFLNK QDMLAEKVLA GKSKIEDYFP
310 320 330 340 350
EYANYTVPED ATPDAGEDPK VTRAKFFIRD LFLRISTATG DGKHYCYPHF
360 370 380
TCAVDTENIR RVFNDCRDII QRMHLKQYEL L
Length:381
Mass (Da):44,248
Last modified:July 15, 1998 - v2
Checksum:i1206E3659457994C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701S → F (PubMed:2499043).Curated
Sequence conflicti90 – 901I → L (PubMed:2499043).Curated
Sequence conflicti94 – 941V → I (PubMed:2499043).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26718 Unassigned DNA. No translation available.
S80376 mRNA. Translation: AAP32223.1.
S80330 mRNA. No translation available.
RefSeqiNP_001178765.1. NM_001191836.2.
UniGeneiRn.203753.
Rn.215691.

Genome annotation databases

GeneIDi24611.
KEGGirno:24611.
UCSCiRGD:2715. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26718 Unassigned DNA. No translation available.
S80376 mRNA. Translation: AAP32223.1.
S80330 mRNA. No translation available.
RefSeqiNP_001178765.1. NM_001191836.2.
UniGeneiRn.203753.
Rn.215691.

3D structure databases

ProteinModelPortaliP38406.
SMRiP38406. Positions 39-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025172.

PTM databases

PhosphoSiteiP38406.

Proteomic databases

PaxDbiP38406.
PRIDEiP38406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24611.
KEGGirno:24611.
UCSCiRGD:2715. rat.

Organism-specific databases

CTDi2774.
RGDi2715. Gnal.

Phylogenomic databases

eggNOGiKOG0099. Eukaryota.
ENOG410XPC4. LUCA.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiP38406.
KOiK04633.
PhylomeDBiP38406.

Miscellaneous databases

PROiP38406.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000367. Gprotein_alpha_S.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00443. GPROTEINAS.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Golf: an olfactory neuron specific-G protein involved in odorant signal transduction."
    Jones D.T., Reed R.R.
    Science 244:790-795(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Molecular analysis of the multiple Golf alpha subunit mRNAs in the rat brain."
    Herve D., Rogard M., Levi-Strauss M.
    Brain Res. Mol. Brain Res. 32:125-134(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiGNAL_RAT
AccessioniPrimary (citable) accession number: P38406
Secondary accession number(s): Q64711
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 15, 1998
Last modified: July 6, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.