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P38361

- PHO89_YEAST

UniProt

P38361 - PHO89_YEAST

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Protein

Phosphate permease PHO89

Gene

PHO89

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Sodium-phosphate symporter. Active in early growth phase.1 Publication

Kineticsi

  1. KM=0.5 µM for phosphate

pH dependencei

Optimum pH is 9.5.

GO - Molecular functioni

  1. identical protein binding Source: IntAct
  2. inorganic phosphate transmembrane transporter activity Source: InterPro
  3. sodium:inorganic phosphate symporter activity Source: SGD

GO - Biological processi

  1. phosphate ion transmembrane transport Source: SGD
  2. phosphate ion transport Source: SGD
  3. sodium ion transmembrane transport Source: GOC
  4. transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29214-MONOMER.
ReactomeiREACT_224271. Sodium-coupled phosphate cotransporters.

Protein family/group databases

TCDBi2.A.20.2.2. the inorganic phosphate transporter (pit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate permease PHO89
Alternative name(s):
Na(+)/Pi cotransporter PHO89
Gene namesi
Name:PHO89
Synonyms:ITN1
Ordered Locus Names:YBR296C
ORF Names:YBR2113
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR296c.
SGDiS000000500. PHO89.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Phosphate permease PHO89PRO_0000080781Add
BLAST

Proteomic databases

MaxQBiP38361.

Expressioni

Inductioni

The gene for PHO89 is only transcribed under conditions of phosphate limitation.

Gene expression databases

GenevestigatoriP38361.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-21068,EBI-21068

Protein-protein interaction databases

BioGridi32989. 24 interactions.
DIPiDIP-4966N.
MINTiMINT-482607.
STRINGi4932.YBR296C.

Structurei

3D structure databases

ProteinModelPortaliP38361.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence AnalysisAdd
BLAST
Transmembranei44 – 6421HelicalSequence AnalysisAdd
BLAST
Transmembranei85 – 10521HelicalSequence AnalysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence AnalysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence AnalysisAdd
BLAST
Transmembranei185 – 20521HelicalSequence AnalysisAdd
BLAST
Transmembranei223 – 24321HelicalSequence AnalysisAdd
BLAST
Transmembranei355 – 37521HelicalSequence AnalysisAdd
BLAST
Transmembranei399 – 41921HelicalSequence AnalysisAdd
BLAST
Transmembranei448 – 46821HelicalSequence AnalysisAdd
BLAST
Transmembranei504 – 52421HelicalSequence AnalysisAdd
BLAST
Transmembranei542 – 56221HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0306.
GeneTreeiENSGT00390000014879.
HOGENOMiHOG000231892.
InParanoidiP38361.
KOiK14640.
OMAiICTMLIV.
OrthoDBiEOG7MPRPM.

Family and domain databases

InterProiIPR001204. Phos_transporter.
[Graphical view]
PANTHERiPTHR11101. PTHR11101. 1 hit.
PfamiPF01384. PHO4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38361-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALHQFDYIF AIAMLFAFLD AFNIGANDVA NSFASSISSR SLKYWQAMVL
60 70 80 90 100
AGLCEFLGAV LAGARVSGTI KNNIIDSSIF TNDPAVLMLT MTSALIGSSC
110 120 130 140 150
WLTFATAIGM PVSTTHSIVG GTIGAGIAAG GANGVVWGWS GVSQIIASWF
160 170 180 190 200
IAPILAGAIA AIVFSISRFS VLEVKSLERS IKNALLLVGV LVFATFSILT
210 220 230 240 250
MLIVWKGSPN LHLDDLSETE TAVSIVLTGA IASIVYFIFF YPFYRRKVLD
260 270 280 290 300
QDWTLKLIDI FRGPSFYFKS TDDIPPMPEG HQLTIDYYEG RRNLGTTVSV
310 320 330 340 350
EDEENKAASN SNDSVKNKED IQEVDLVRTE TEPETKLSTK QYWWSLLKQG
360 370 380 390 400
PKKWPLLFWL VISHGWTQDV IHAQVNDRDM LSGDLKGMYE RSKFYDNRVE
410 420 430 440 450
YIYSVLQAIT AATMSFAHGA NDVANATGPL SAVYVIWKTN TIGAKSEVPV
460 470 480 490 500
WVLAYGGVAL VIGCWTYGYN IIKNLGNKMI LQSPSRGFSI ELAVAITTVM
510 520 530 540 550
ATQLGIPTST TQIAVGGIVA VGLCNKDLKS VNWRMVAWCY SGWFLTLPIA
560 570
GLIAGIINGI ILNAPRFGVE YQMT
Length:574
Mass (Da):62,654
Last modified:October 1, 1994 - v1
Checksum:i9794C1D9D75014D5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36165 Genomic DNA. Translation: CAA85261.1.
BK006936 Genomic DNA. Translation: DAA07410.1.
PIRiS46178.
RefSeqiNP_009855.1. NM_001178644.1.

Genome annotation databases

EnsemblFungiiYBR296C; YBR296C; YBR296C.
GeneIDi852599.
KEGGisce:YBR296C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36165 Genomic DNA. Translation: CAA85261.1 .
BK006936 Genomic DNA. Translation: DAA07410.1 .
PIRi S46178.
RefSeqi NP_009855.1. NM_001178644.1.

3D structure databases

ProteinModelPortali P38361.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32989. 24 interactions.
DIPi DIP-4966N.
MINTi MINT-482607.
STRINGi 4932.YBR296C.

Protein family/group databases

TCDBi 2.A.20.2.2. the inorganic phosphate transporter (pit) family.

Proteomic databases

MaxQBi P38361.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR296C ; YBR296C ; YBR296C .
GeneIDi 852599.
KEGGi sce:YBR296C.

Organism-specific databases

CYGDi YBR296c.
SGDi S000000500. PHO89.

Phylogenomic databases

eggNOGi COG0306.
GeneTreei ENSGT00390000014879.
HOGENOMi HOG000231892.
InParanoidi P38361.
KOi K14640.
OMAi ICTMLIV.
OrthoDBi EOG7MPRPM.

Enzyme and pathway databases

BioCyci YEAST:G3O-29214-MONOMER.
Reactomei REACT_224271. Sodium-coupled phosphate cotransporters.

Miscellaneous databases

NextBioi 971775.

Gene expression databases

Genevestigatori P38361.

Family and domain databases

InterProi IPR001204. Phos_transporter.
[Graphical view ]
PANTHERi PTHR11101. PTHR11101. 1 hit.
Pfami PF01384. PHO4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification, cloning and characterization of a derepressible Na+-coupled phosphate transporter in Saccharomyces cerevisiae."
    Martinez P., Persson B.L.
    Mol. Gen. Genet. 258:628-638(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CHARACTERIZATION.
  4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPHO89_YEAST
AccessioniPrimary (citable) accession number: P38361
Secondary accession number(s): D6VQU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3