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Protein

Phosphate permease PHO89

Gene

PHO89

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-phosphate symporter. Active in early growth phase.1 Publication

Kineticsi

  1. KM=0.5 µM for phosphate

    pH dependencei

    Optimum pH is 9.5.

    GO - Molecular functioni

    • identical protein binding Source: IntAct
    • inorganic phosphate transmembrane transporter activity Source: InterPro
    • sodium:inorganic phosphate symporter activity Source: SGD

    GO - Biological processi

    • phosphate ion transmembrane transport Source: SGD
    • phosphate ion transport Source: SGD
    • sodium ion transmembrane transport Source: GOC
    • transmembrane transport Source: SGD
    Complete GO annotation...

    Keywords - Biological processi

    Symport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29214-MONOMER.
    ReactomeiREACT_285302. Sodium-coupled phosphate cotransporters.

    Protein family/group databases

    TCDBi2.A.20.2.2. the inorganic phosphate transporter (pit) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphate permease PHO89
    Alternative name(s):
    Na(+)/Pi cotransporter PHO89
    Gene namesi
    Name:PHO89
    Synonyms:ITN1
    Ordered Locus Names:YBR296C
    ORF Names:YBR2113
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311 Componenti: Chromosome II

    Organism-specific databases

    CYGDiYBR296c.
    EuPathDBiFungiDB:YBR296C.
    SGDiS000000500. PHO89.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei44 – 6421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei85 – 10521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei185 – 20521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei223 – 24321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei355 – 37521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei399 – 41921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei448 – 46821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei504 – 52421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei542 – 56221HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Phosphate permease PHO89PRO_0000080781Add
    BLAST

    Proteomic databases

    MaxQBiP38361.

    Expressioni

    Inductioni

    The gene for PHO89 is only transcribed under conditions of phosphate limitation.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-21068,EBI-21068

    Protein-protein interaction databases

    BioGridi32989. 24 interactions.
    DIPiDIP-4966N.
    MINTiMINT-482607.
    STRINGi4932.YBR296C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38361.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0306.
    GeneTreeiENSGT00390000014879.
    HOGENOMiHOG000231892.
    InParanoidiP38361.
    KOiK14640.
    OMAiCAGSENA.
    OrthoDBiEOG7MPRPM.

    Family and domain databases

    InterProiIPR001204. Phos_transporter.
    [Graphical view]
    PANTHERiPTHR11101. PTHR11101. 1 hit.
    PfamiPF01384. PHO4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38361-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MALHQFDYIF AIAMLFAFLD AFNIGANDVA NSFASSISSR SLKYWQAMVL
    60 70 80 90 100
    AGLCEFLGAV LAGARVSGTI KNNIIDSSIF TNDPAVLMLT MTSALIGSSC
    110 120 130 140 150
    WLTFATAIGM PVSTTHSIVG GTIGAGIAAG GANGVVWGWS GVSQIIASWF
    160 170 180 190 200
    IAPILAGAIA AIVFSISRFS VLEVKSLERS IKNALLLVGV LVFATFSILT
    210 220 230 240 250
    MLIVWKGSPN LHLDDLSETE TAVSIVLTGA IASIVYFIFF YPFYRRKVLD
    260 270 280 290 300
    QDWTLKLIDI FRGPSFYFKS TDDIPPMPEG HQLTIDYYEG RRNLGTTVSV
    310 320 330 340 350
    EDEENKAASN SNDSVKNKED IQEVDLVRTE TEPETKLSTK QYWWSLLKQG
    360 370 380 390 400
    PKKWPLLFWL VISHGWTQDV IHAQVNDRDM LSGDLKGMYE RSKFYDNRVE
    410 420 430 440 450
    YIYSVLQAIT AATMSFAHGA NDVANATGPL SAVYVIWKTN TIGAKSEVPV
    460 470 480 490 500
    WVLAYGGVAL VIGCWTYGYN IIKNLGNKMI LQSPSRGFSI ELAVAITTVM
    510 520 530 540 550
    ATQLGIPTST TQIAVGGIVA VGLCNKDLKS VNWRMVAWCY SGWFLTLPIA
    560 570
    GLIAGIINGI ILNAPRFGVE YQMT
    Length:574
    Mass (Da):62,654
    Last modified:October 1, 1994 - v1
    Checksum:i9794C1D9D75014D5
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z36165 Genomic DNA. Translation: CAA85261.1.
    BK006936 Genomic DNA. Translation: DAA07410.1.
    PIRiS46178.
    RefSeqiNP_009855.1. NM_001178644.1.

    Genome annotation databases

    EnsemblFungiiYBR296C; YBR296C; YBR296C.
    GeneIDi852599.
    KEGGisce:YBR296C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z36165 Genomic DNA. Translation: CAA85261.1.
    BK006936 Genomic DNA. Translation: DAA07410.1.
    PIRiS46178.
    RefSeqiNP_009855.1. NM_001178644.1.

    3D structure databases

    ProteinModelPortaliP38361.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi32989. 24 interactions.
    DIPiDIP-4966N.
    MINTiMINT-482607.
    STRINGi4932.YBR296C.

    Protein family/group databases

    TCDBi2.A.20.2.2. the inorganic phosphate transporter (pit) family.

    Proteomic databases

    MaxQBiP38361.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYBR296C; YBR296C; YBR296C.
    GeneIDi852599.
    KEGGisce:YBR296C.

    Organism-specific databases

    CYGDiYBR296c.
    EuPathDBiFungiDB:YBR296C.
    SGDiS000000500. PHO89.

    Phylogenomic databases

    eggNOGiCOG0306.
    GeneTreeiENSGT00390000014879.
    HOGENOMiHOG000231892.
    InParanoidiP38361.
    KOiK14640.
    OMAiCAGSENA.
    OrthoDBiEOG7MPRPM.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29214-MONOMER.
    ReactomeiREACT_285302. Sodium-coupled phosphate cotransporters.

    Miscellaneous databases

    NextBioi971775.
    PROiP38361.

    Family and domain databases

    InterProiIPR001204. Phos_transporter.
    [Graphical view]
    PANTHERiPTHR11101. PTHR11101. 1 hit.
    PfamiPF01384. PHO4. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Identification, cloning and characterization of a derepressible Na+-coupled phosphate transporter in Saccharomyces cerevisiae."
      Martinez P., Persson B.L.
      Mol. Gen. Genet. 258:628-638(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CHARACTERIZATION.
    4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPHO89_YEAST
    AccessioniPrimary (citable) accession number: P38361
    Secondary accession number(s): D6VQU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: June 24, 2015
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.