Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P38361

- PHO89_YEAST

UniProt

P38361 - PHO89_YEAST

Protein

Phosphate permease PHO89

Gene

PHO89

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sodium-phosphate symporter. Active in early growth phase.1 Publication

    Kineticsi

    1. KM=0.5 µM for phosphate

    pH dependencei

    Optimum pH is 9.5.

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. inorganic phosphate transmembrane transporter activity Source: InterPro
    3. sodium:inorganic phosphate symporter activity Source: SGD

    GO - Biological processi

    1. phosphate ion transmembrane transport Source: SGD
    2. phosphate ion transport Source: SGD
    3. sodium ion transmembrane transport Source: GOC
    4. transmembrane transport Source: SGD

    Keywords - Biological processi

    Symport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29214-MONOMER.
    ReactomeiREACT_224271. Sodium-coupled phosphate cotransporters.

    Protein family/group databases

    TCDBi2.A.20.2.2. the inorganic phosphate transporter (pit) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphate permease PHO89
    Alternative name(s):
    Na(+)/Pi cotransporter PHO89
    Gene namesi
    Name:PHO89
    Synonyms:ITN1
    Ordered Locus Names:YBR296C
    ORF Names:YBR2113
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR296c.
    SGDiS000000500. PHO89.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Phosphate permease PHO89PRO_0000080781Add
    BLAST

    Proteomic databases

    MaxQBiP38361.

    Expressioni

    Inductioni

    The gene for PHO89 is only transcribed under conditions of phosphate limitation.

    Gene expression databases

    GenevestigatoriP38361.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-21068,EBI-21068

    Protein-protein interaction databases

    BioGridi32989. 24 interactions.
    DIPiDIP-4966N.
    MINTiMINT-482607.
    STRINGi4932.YBR296C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38361.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei44 – 6421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei85 – 10521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei118 – 13821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei185 – 20521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei223 – 24321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei355 – 37521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei399 – 41921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei448 – 46821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei504 – 52421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei542 – 56221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0306.
    GeneTreeiENSGT00390000014879.
    HOGENOMiHOG000231892.
    KOiK14640.
    OMAiICTMLIV.
    OrthoDBiEOG7MPRPM.

    Family and domain databases

    InterProiIPR001204. Phos_transporter.
    [Graphical view]
    PANTHERiPTHR11101. PTHR11101. 1 hit.
    PfamiPF01384. PHO4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38361-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALHQFDYIF AIAMLFAFLD AFNIGANDVA NSFASSISSR SLKYWQAMVL    50
    AGLCEFLGAV LAGARVSGTI KNNIIDSSIF TNDPAVLMLT MTSALIGSSC 100
    WLTFATAIGM PVSTTHSIVG GTIGAGIAAG GANGVVWGWS GVSQIIASWF 150
    IAPILAGAIA AIVFSISRFS VLEVKSLERS IKNALLLVGV LVFATFSILT 200
    MLIVWKGSPN LHLDDLSETE TAVSIVLTGA IASIVYFIFF YPFYRRKVLD 250
    QDWTLKLIDI FRGPSFYFKS TDDIPPMPEG HQLTIDYYEG RRNLGTTVSV 300
    EDEENKAASN SNDSVKNKED IQEVDLVRTE TEPETKLSTK QYWWSLLKQG 350
    PKKWPLLFWL VISHGWTQDV IHAQVNDRDM LSGDLKGMYE RSKFYDNRVE 400
    YIYSVLQAIT AATMSFAHGA NDVANATGPL SAVYVIWKTN TIGAKSEVPV 450
    WVLAYGGVAL VIGCWTYGYN IIKNLGNKMI LQSPSRGFSI ELAVAITTVM 500
    ATQLGIPTST TQIAVGGIVA VGLCNKDLKS VNWRMVAWCY SGWFLTLPIA 550
    GLIAGIINGI ILNAPRFGVE YQMT 574
    Length:574
    Mass (Da):62,654
    Last modified:October 1, 1994 - v1
    Checksum:i9794C1D9D75014D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36165 Genomic DNA. Translation: CAA85261.1.
    BK006936 Genomic DNA. Translation: DAA07410.1.
    PIRiS46178.
    RefSeqiNP_009855.1. NM_001178644.1.

    Genome annotation databases

    EnsemblFungiiYBR296C; YBR296C; YBR296C.
    GeneIDi852599.
    KEGGisce:YBR296C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36165 Genomic DNA. Translation: CAA85261.1 .
    BK006936 Genomic DNA. Translation: DAA07410.1 .
    PIRi S46178.
    RefSeqi NP_009855.1. NM_001178644.1.

    3D structure databases

    ProteinModelPortali P38361.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32989. 24 interactions.
    DIPi DIP-4966N.
    MINTi MINT-482607.
    STRINGi 4932.YBR296C.

    Protein family/group databases

    TCDBi 2.A.20.2.2. the inorganic phosphate transporter (pit) family.

    Proteomic databases

    MaxQBi P38361.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR296C ; YBR296C ; YBR296C .
    GeneIDi 852599.
    KEGGi sce:YBR296C.

    Organism-specific databases

    CYGDi YBR296c.
    SGDi S000000500. PHO89.

    Phylogenomic databases

    eggNOGi COG0306.
    GeneTreei ENSGT00390000014879.
    HOGENOMi HOG000231892.
    KOi K14640.
    OMAi ICTMLIV.
    OrthoDBi EOG7MPRPM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29214-MONOMER.
    Reactomei REACT_224271. Sodium-coupled phosphate cotransporters.

    Miscellaneous databases

    NextBioi 971775.

    Gene expression databases

    Genevestigatori P38361.

    Family and domain databases

    InterProi IPR001204. Phos_transporter.
    [Graphical view ]
    PANTHERi PTHR11101. PTHR11101. 1 hit.
    Pfami PF01384. PHO4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Identification, cloning and characterization of a derepressible Na+-coupled phosphate transporter in Saccharomyces cerevisiae."
      Martinez P., Persson B.L.
      Mol. Gen. Genet. 258:628-638(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CHARACTERIZATION.
    4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    5. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPHO89_YEAST
    AccessioniPrimary (citable) accession number: P38361
    Secondary accession number(s): D6VQU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3