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P38361 (PHO89_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphate permease PHO89
Alternative name(s):
Na(+)/Pi cotransporter PHO89
Gene names
Name:PHO89
Synonyms:ITN1
Ordered Locus Names:YBR296C
ORF Names:YBR2113
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-phosphate symporter. Active in early growth phase. Ref.3

Subcellular location

Membrane; Multi-pass membrane protein.

Induction

The gene for PHO89 is only transcribed under conditions of phosphate limitation.

Sequence similarities

Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=0.5 µM for phosphate

pH dependence:

Optimum pH is 9.5.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself4EBI-21068,EBI-21068

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 574574Phosphate permease PHO89
PRO_0000080781

Regions

Transmembrane6 – 2621Helical; Potential
Transmembrane44 – 6421Helical; Potential
Transmembrane85 – 10521Helical; Potential
Transmembrane118 – 13821Helical; Potential
Transmembrane146 – 16621Helical; Potential
Transmembrane185 – 20521Helical; Potential
Transmembrane223 – 24321Helical; Potential
Transmembrane355 – 37521Helical; Potential
Transmembrane399 – 41921Helical; Potential
Transmembrane448 – 46821Helical; Potential
Transmembrane504 – 52421Helical; Potential
Transmembrane542 – 56221Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
P38361 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 9794C1D9D75014D5

FASTA57462,654
        10         20         30         40         50         60 
MALHQFDYIF AIAMLFAFLD AFNIGANDVA NSFASSISSR SLKYWQAMVL AGLCEFLGAV 

        70         80         90        100        110        120 
LAGARVSGTI KNNIIDSSIF TNDPAVLMLT MTSALIGSSC WLTFATAIGM PVSTTHSIVG 

       130        140        150        160        170        180 
GTIGAGIAAG GANGVVWGWS GVSQIIASWF IAPILAGAIA AIVFSISRFS VLEVKSLERS 

       190        200        210        220        230        240 
IKNALLLVGV LVFATFSILT MLIVWKGSPN LHLDDLSETE TAVSIVLTGA IASIVYFIFF 

       250        260        270        280        290        300 
YPFYRRKVLD QDWTLKLIDI FRGPSFYFKS TDDIPPMPEG HQLTIDYYEG RRNLGTTVSV 

       310        320        330        340        350        360 
EDEENKAASN SNDSVKNKED IQEVDLVRTE TEPETKLSTK QYWWSLLKQG PKKWPLLFWL 

       370        380        390        400        410        420 
VISHGWTQDV IHAQVNDRDM LSGDLKGMYE RSKFYDNRVE YIYSVLQAIT AATMSFAHGA 

       430        440        450        460        470        480 
NDVANATGPL SAVYVIWKTN TIGAKSEVPV WVLAYGGVAL VIGCWTYGYN IIKNLGNKMI 

       490        500        510        520        530        540 
LQSPSRGFSI ELAVAITTVM ATQLGIPTST TQIAVGGIVA VGLCNKDLKS VNWRMVAWCY 

       550        560        570 
SGWFLTLPIA GLIAGIINGI ILNAPRFGVE YQMT 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Identification, cloning and characterization of a derepressible Na+-coupled phosphate transporter in Saccharomyces cerevisiae."
Martinez P., Persson B.L.
Mol. Gen. Genet. 258:628-638(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CHARACTERIZATION.
[4]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[5]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z36165 Genomic DNA. Translation: CAA85261.1.
BK006936 Genomic DNA. Translation: DAA07410.1.
PIRS46178.
RefSeqNP_009855.1. NM_001178644.1.

3D structure databases

ProteinModelPortalP38361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32989. 24 interactions.
DIPDIP-4966N.
MINTMINT-482607.
STRING4932.YBR296C.

Protein family/group databases

TCDB2.A.20.2.2. the inorganic phosphate transporter (pit) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR296C; YBR296C; YBR296C.
GeneID852599.
KEGGsce:YBR296C.

Organism-specific databases

CYGDYBR296c.
SGDS000000500. PHO89.

Phylogenomic databases

eggNOGCOG0306.
GeneTreeENSGT00390000014879.
HOGENOMHOG000231892.
KOK14640.
OMAICTMLIV.
OrthoDBEOG7MPRPM.

Enzyme and pathway databases

BioCycYEAST:G3O-29214-MONOMER.

Gene expression databases

GenevestigatorP38361.

Family and domain databases

InterProIPR001204. Phos_transporter.
[Graphical view]
PANTHERPTHR11101. PTHR11101. 1 hit.
PfamPF01384. PHO4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971775.

Entry information

Entry namePHO89_YEAST
AccessionPrimary (citable) accession number: P38361
Secondary accession number(s): D6VQU0
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families