Reviewed, UniProtKB/Swiss-Prot P38360 (ATU1_YEAST)

Names and origin

Protein names

Recommended name:
    Probable copper-transporting ATPase
    EC=3.6.3.4
Alternative name(s):
    Cu(2+)-ATPase

Gene names

Name:	PCA1
Ordered Locus Names:	YBR295W
ORF Names:	YBR2112

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1216 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probably involved in copper transport and in the regulation of cellular copper level.
Catalytic activity	ATP + H(2)O + Cu(2+)(In) = ADP + phosphate + Cu(2+)(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IB subfamily. Contains 1 HMA domain.

Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Copper Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cadmium ion transport Inferred from genetic interaction. Source: SGD cellular iron ion homeostasis Inferred from expression pattern. Source: SGD cellular metal ion homeostasis Inferred from mutant phenotype. Source: SGD copper ion transport Inferred from electronic annotation. Source: UniProtKB-KW metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro cadmium-exporting ATPase activity Inferred from mutant phenotype. Source: SGD copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW copper-exporting ATPase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1216	1216	Probable copper-transporting ATPase		PRO_0000046317
Regions
	Topological domain	1 – 556	556	Cytoplasmic Potential
	Transmembrane	557 – 578	22	Potential
	Topological domain	579 – 592	14	Extracellular Potential
	Transmembrane	593 – 612	20	Potential
	Topological domain	613 – 620	8	Cytoplasmic Potential
	Transmembrane	621 – 641	21	Potential
	Topological domain	642 – 659	18	Extracellular Potential
	Transmembrane	660 – 680	21	Potential
	Topological domain	681 – 808	128	Cytoplasmic Potential
	Transmembrane	809 – 831	23	Potential
	Topological domain	832 – 847	16	Extracellular Potential
	Transmembrane	848 – 865	18	Potential
	Topological domain	866 – 1161	296	Cytoplasmic Potential
	Transmembrane	1162 – 1181	20	Potential
	Topological domain	1182 – 1190	9	Extracellular Potential
	Transmembrane	1191 – 1209	19	Potential
	Topological domain	1210 – 1216	7	Cytoplasmic Potential
	Domain	411 – 474	64	HMA
Sites
	Active site	903	1	4-aspartylphosphate intermediate By similarity
	Metal binding	421	1	Copper Potential
	Metal binding	424	1	Copper Potential
	Metal binding	1107	1	Magnesium By similarity
	Metal binding	1111	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38360-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: B0BA4D606D75F9EA	FASTA	1,216	131,839
	10 20 30 40 50 60 MKPEKLFSGL GTSDGEYGVV NSENISIDAM QDNRGECHRR SIEMHANDNL GLVSQRDCTN 70 80 90 100 110 120 RPKITPQECL SETEQICHHG ENRTKAGLDV DDAETGGDHT NESRVDECCA EKVNDTETGL 130 140 150 160 170 180 DVDSCCGDAQ TGGDHTNESC VDGCCVRDSS VMVEEVTGSC EAVSSKEQLL TSFEVVPSKS 190 200 210 220 230 240 EGLQSIHDIR ETTRCNTNSN QHTGKGRLCI ESSDSTLKKR SCKVSRQKIE VSSKPECCNI 250 260 270 280 290 300 SCVERIASRS CEKRTFKGST NVGISGSSST DSLSEKFFSE QYSRMYNRYS SILKNLGCIC 310 320 330 340 350 360 NYLRTLGKES CCLPKVRFCS GEGASKKTKY SYRNSSGCLT KKKTHGDKER LSNDNGHADF 370 380 390 400 410 420 VCSKSCCTKM KDCAVTSTIS GTSSSEISRI VSMEPIENHL NLEAGSTGTE HIVLSVSGMS 430 440 450 460 470 480 CTGCESKLKK SFGALKCVHG LKTSLILSQA EFNLDLAQGS VKDVIKHLSK TTEFKYEQIS 490 500 510 520 530 540 NHGSTIDVVV PYAAKDFINE EWPQGVTELK IVERNIIRIY FDPKVIGARD LVNEGWSVPV 550 560 570 580 590 600 SIAPFSCHPT IEVGRKHLVR VGCTTALSII LTIPILVMAW APQLREKIST ISASMVLATI 610 620 630 640 650 660 IQFVIAGPFY LNALKSLIFS RLIEMDLLIV LSTSAAYIFS IVSFGYFVVG RPLSTEQFFE 670 680 690 700 710 720 TSSLLVTLIM VGRFVSELAR HRAVKSISVR SLQASSAILV DKTGKETEIN IRLLQYGDIF 730 740 750 760 770 780 KVLPDSRIPT DGTVISGSSE VDEALITGES MPVPKKCQSI VVAGSVNGTG TLFVKLSKLP 790 800 810 820 830 840 GNNTISTIAT MVDEAKLTKP KIQNIADKIA SYFVPTIIGI TVVTFCVWIA VGIRVEKQSR 850 860 870 880 890 900 SDAVIQAIIY AITVLIVSCP CVIGLAVPIV FVIASGVAAK RGVIFKSAES IEVAHNTSHV 910 920 930 940 950 960 VFDKTGTLTE GKLTVVHETV RGDRHNSQSL LLGLTEGIKH PVSMAIASYL KEKGVSAQNV 970 980 990 1000 1010 1020 SNTKAVTGKR VEGTSYSGLK LQGGNCRWLG HNNDPDVRKA LEQGYSVFCF SVNGSVTAVY 1030 1040 1050 1060 1070 1080 ALEDSLRADA VSTINLLRQR GISLHILSGD DDGAVRSMAA RLGIESSNIR SHATPAEKSE 1090 1100 1110 1120 1130 1140 YIKDIVEGRN CDSSSQSKRP VVVFCGDGTN DAIGLTQATI GVHINEGSEV AKLAADVVML 1150 1160 1170 1180 1190 1200 KPKLNNILTM ITVSQKAMFR VKLNFLWSFT YNLFAILLAA GAFVDFHIPP EYAGLGELVS 1210 ILPVIFVAIL LRYAKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A putative P-type Cu(2+)-transporting ATPase gene on chromosome II of Saccharomyces cerevisiae." Rad M.R., Kirchrath L., Hollenberg C. Yeast 10:1217-1225(1994) [PubMed: 7754711] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z29332 Genomic DNA. Translation: CAA82529.1. Z36164 Genomic DNA. Translation: CAA85260.1.
PIR	S46177.
RefSeq	NP_009854.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6641N.
Genome annotation databases
Ensembl	YBR295W. Saccharomyces cerevisiae. [Contig view]
GeneID	852598.
GenomeReviews	Gene locus YBR295W in contig Y13134_GR.
KEGG	sce:YBR295W.
NMPDR	fig|4932.3.peg.570.
Organism-specific databases
CYGD	YBR295w.
SGD	S000000499. PCA1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38360.
Gene expression databases
ArrayExpress	P38360.
GermOnline	YBR295W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006416. ATPase-IB_hvy. IPR001757. ATPase_P. IPR006403. ATPase_P_cat/Cu. IPR005834. Dehalogen-like_hydro. IPR008250. E1-E2_ATPase_reg. IPR006121. HeavyMe_transpt. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. False negative. PS50846. HMA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P38360.
NextBio	971772.

Entry information

Entry name

ATU1_YEAST

Accession

Primary (citable) accession number: P38360

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 25, 2008
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)