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Protein

Vacuolar basic amino acid transporter 2

Gene

VBA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter required for vacuolar uptake of histidine, arginine and lysine and to a lesser extent tyrosine.1 Publication

GO - Molecular functioni

  • basic amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • basic amino acid transport Source: SGD
  • transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29211-MONOMER.

Protein family/group databases

TCDBi2.A.1.48.2. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar basic amino acid transporter 2
Gene namesi
Name:VBA2
Ordered Locus Names:YBR293W
ORF Names:YBR2109
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR293W.
SGDiS000000497. VBA2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33CytoplasmicSequence analysisAdd BLAST33
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 62VacuolarSequence analysis8
Transmembranei63 – 85HelicalSequence analysisAdd BLAST23
Topological domaini86 – 97CytoplasmicSequence analysisAdd BLAST12
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 121VacuolarSequence analysis3
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 167CytoplasmicSequence analysisAdd BLAST25
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Topological domaini189 – 196VacuolarSequence analysis8
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Topological domaini218 – 238CytoplasmicSequence analysisAdd BLAST21
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 273VacuolarSequence analysisAdd BLAST14
Transmembranei274 – 294HelicalSequence analysisAdd BLAST21
Topological domaini295 – 303CytoplasmicSequence analysis9
Transmembranei304 – 324HelicalSequence analysisAdd BLAST21
Topological domaini325 – 331VacuolarSequence analysis7
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
Topological domaini353 – 375CytoplasmicSequence analysisAdd BLAST23
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21
Topological domaini397 – 447VacuolarSequence analysisAdd BLAST51
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 474CytoplasmicSequence analysis6

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001734231 – 474Vacuolar basic amino acid transporter 2Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi420N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP38358.

Interactioni

Protein-protein interaction databases

BioGridi32986. 21 interactors.
DIPiDIP-5142N.
IntActiP38358. 4 interactors.
MINTiMINT-503565.

Structurei

3D structure databases

ProteinModelPortaliP38358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000165564.
InParanoidiP38358.
OMAiGVAFNPM.
OrthoDBiEOG092C21WF.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISNWITTA YLITSTSFQP LYGSFSDALG RRNCLFFANG AFTIGCLACG
60 70 80 90 100
FSKNIYMLSF MRALTGIGGG GLITLSTIVN SDVIPSSKRG IFQAFQNLLL
110 120 130 140 150
GFGAICGASF GGTIASSIGW RWCFLIQVPI SVISSILMNY YVPNQKEYNR
160 170 180 190 200
QNSSIFQNPG KILRDIDVMG SILIITGLTL QLLYLSLGCS TSKLSWTSPS
210 220 230 240 250
VLLLLVGSVI ILLLFILHER KTSARAIIPM ELVNSSYSVV VLSISILVGF
260 270 280 290 300
ASYAYLFTLP LFFQIVLGDS TAKAGLRLTI PSLFTPVGSL ITGFSMSKYN
310 320 330 340 350
CLRLLLYIGI SLMFLGNFLF LFIEKTSPNW LIGLFLIPAN LGQGITFPTT
360 370 380 390 400
LFTFIFMFSK SDQATATSTL YLFRSIGSVW GVAISAGVIQ LSFAGLLRSN
410 420 430 440 450
LKGLLDENKI KKLIVQLSAN SSYIGSLHGE VKNTVIKSFD EATKRAHLMS
460 470
TLLSSLALIL CILKDNLAKP KTRR
Length:474
Mass (Da):51,677
Last modified:October 1, 1994 - v1
Checksum:i1F3FDC877795ACFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36162 Genomic DNA. Translation: CAA85258.1.
AY692630 Genomic DNA. Translation: AAT92649.1.
BK006936 Genomic DNA. Translation: DAA07407.1.
PIRiS46175.
RefSeqiNP_009852.3. NM_001178641.3.

Genome annotation databases

EnsemblFungiiYBR293W; YBR293W; YBR293W.
GeneIDi852596.
KEGGisce:YBR293W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36162 Genomic DNA. Translation: CAA85258.1.
AY692630 Genomic DNA. Translation: AAT92649.1.
BK006936 Genomic DNA. Translation: DAA07407.1.
PIRiS46175.
RefSeqiNP_009852.3. NM_001178641.3.

3D structure databases

ProteinModelPortaliP38358.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32986. 21 interactors.
DIPiDIP-5142N.
IntActiP38358. 4 interactors.
MINTiMINT-503565.

Protein family/group databases

TCDBi2.A.1.48.2. the major facilitator superfamily (mfs).

Proteomic databases

PRIDEiP38358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR293W; YBR293W; YBR293W.
GeneIDi852596.
KEGGisce:YBR293W.

Organism-specific databases

EuPathDBiFungiDB:YBR293W.
SGDiS000000497. VBA2.

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000165564.
InParanoidiP38358.
OMAiGVAFNPM.
OrthoDBiEOG092C21WF.

Enzyme and pathway databases

BioCyciYEAST:G3O-29211-MONOMER.

Miscellaneous databases

PROiP38358.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVBA2_YEAST
AccessioniPrimary (citable) accession number: P38358
Secondary accession number(s): D6VQT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.