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Protein

Vacuolar basic amino acid transporter 2

Gene

VBA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter required for vacuolar uptake of histidine, arginine and lysine and to a lesser extent tyrosine.1 Publication

GO - Molecular functioni

  • basic amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • basic amino acid transport Source: SGD
  • transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29211-MONOMER.

Protein family/group databases

TCDBi2.A.1.48.2. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar basic amino acid transporter 2
Gene namesi
Name:VBA2
Ordered Locus Names:YBR293W
ORF Names:YBR2109
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR293W.
SGDiS000000497. VBA2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence analysisAdd
BLAST
Transmembranei34 – 5421HelicalSequence analysisAdd
BLAST
Topological domaini55 – 628VacuolarSequence analysis
Transmembranei63 – 8523HelicalSequence analysisAdd
BLAST
Topological domaini86 – 9712CytoplasmicSequence analysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST
Topological domaini119 – 1213VacuolarSequence analysis
Transmembranei122 – 14221HelicalSequence analysisAdd
BLAST
Topological domaini143 – 16725CytoplasmicSequence analysisAdd
BLAST
Transmembranei168 – 18821HelicalSequence analysisAdd
BLAST
Topological domaini189 – 1968VacuolarSequence analysis
Transmembranei197 – 21721HelicalSequence analysisAdd
BLAST
Topological domaini218 – 23821CytoplasmicSequence analysisAdd
BLAST
Transmembranei239 – 25921HelicalSequence analysisAdd
BLAST
Topological domaini260 – 27314VacuolarSequence analysisAdd
BLAST
Transmembranei274 – 29421HelicalSequence analysisAdd
BLAST
Topological domaini295 – 3039CytoplasmicSequence analysis
Transmembranei304 – 32421HelicalSequence analysisAdd
BLAST
Topological domaini325 – 3317VacuolarSequence analysis
Transmembranei332 – 35221HelicalSequence analysisAdd
BLAST
Topological domaini353 – 37523CytoplasmicSequence analysisAdd
BLAST
Transmembranei376 – 39621HelicalSequence analysisAdd
BLAST
Topological domaini397 – 44751VacuolarSequence analysisAdd
BLAST
Transmembranei448 – 46821HelicalSequence analysisAdd
BLAST
Topological domaini469 – 4746CytoplasmicSequence analysis

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Vacuolar basic amino acid transporter 2PRO_0000173423Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi420 – 4201N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Interactioni

Protein-protein interaction databases

BioGridi32986. 21 interactions.
DIPiDIP-5142N.
IntActiP38358. 4 interactions.
MINTiMINT-503565.

Structurei

3D structure databases

ProteinModelPortaliP38358.
SMRiP38358. Positions 23-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000165564.
InParanoidiP38358.
OMAiWIETALW.
OrthoDBiEOG74FF92.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISNWITTA YLITSTSFQP LYGSFSDALG RRNCLFFANG AFTIGCLACG
60 70 80 90 100
FSKNIYMLSF MRALTGIGGG GLITLSTIVN SDVIPSSKRG IFQAFQNLLL
110 120 130 140 150
GFGAICGASF GGTIASSIGW RWCFLIQVPI SVISSILMNY YVPNQKEYNR
160 170 180 190 200
QNSSIFQNPG KILRDIDVMG SILIITGLTL QLLYLSLGCS TSKLSWTSPS
210 220 230 240 250
VLLLLVGSVI ILLLFILHER KTSARAIIPM ELVNSSYSVV VLSISILVGF
260 270 280 290 300
ASYAYLFTLP LFFQIVLGDS TAKAGLRLTI PSLFTPVGSL ITGFSMSKYN
310 320 330 340 350
CLRLLLYIGI SLMFLGNFLF LFIEKTSPNW LIGLFLIPAN LGQGITFPTT
360 370 380 390 400
LFTFIFMFSK SDQATATSTL YLFRSIGSVW GVAISAGVIQ LSFAGLLRSN
410 420 430 440 450
LKGLLDENKI KKLIVQLSAN SSYIGSLHGE VKNTVIKSFD EATKRAHLMS
460 470
TLLSSLALIL CILKDNLAKP KTRR
Length:474
Mass (Da):51,677
Last modified:October 1, 1994 - v1
Checksum:i1F3FDC877795ACFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36162 Genomic DNA. Translation: CAA85258.1.
AY692630 Genomic DNA. Translation: AAT92649.1.
BK006936 Genomic DNA. Translation: DAA07407.1.
PIRiS46175.
RefSeqiNP_009852.3. NM_001178641.3.

Genome annotation databases

EnsemblFungiiYBR293W; YBR293W; YBR293W.
GeneIDi852596.
KEGGisce:YBR293W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36162 Genomic DNA. Translation: CAA85258.1.
AY692630 Genomic DNA. Translation: AAT92649.1.
BK006936 Genomic DNA. Translation: DAA07407.1.
PIRiS46175.
RefSeqiNP_009852.3. NM_001178641.3.

3D structure databases

ProteinModelPortaliP38358.
SMRiP38358. Positions 23-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32986. 21 interactions.
DIPiDIP-5142N.
IntActiP38358. 4 interactions.
MINTiMINT-503565.

Protein family/group databases

TCDBi2.A.1.48.2. the major facilitator superfamily (mfs).

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR293W; YBR293W; YBR293W.
GeneIDi852596.
KEGGisce:YBR293W.

Organism-specific databases

EuPathDBiFungiDB:YBR293W.
SGDiS000000497. VBA2.

Phylogenomic databases

GeneTreeiENSGT00390000006559.
HOGENOMiHOG000165564.
InParanoidiP38358.
OMAiWIETALW.
OrthoDBiEOG74FF92.

Enzyme and pathway databases

BioCyciYEAST:G3O-29211-MONOMER.

Miscellaneous databases

PROiP38358.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "A family of basic amino acid transporters of the vacuolar membrane from Saccharomyces cerevisiae."
    Shimazu M., Sekito T., Akiyama K., Ohsumi Y., Kakinuma Y.
    J. Biol. Chem. 280:4851-4857(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiVBA2_YEAST
AccessioniPrimary (citable) accession number: P38358
Secondary accession number(s): D6VQT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 6, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.