P38355 (YB8B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized transporter YBR287W

Gene names

Ordered Locus Names:	YBR287W
ORF Names:	YBR2034

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 6440 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the auxin efflux carrier (TC 2.A.69) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 14562095. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Uncharacterized transporter YBR287W		PRO_0000123805
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	43 – 63	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	253 – 273	21	Helical; Potential
	Transmembrane	288 – 308	21	Helical; Potential
	Transmembrane	327 – 347	21	Helical; Potential
	Transmembrane	358 – 378	21	Helical; Potential
	Transmembrane	397 – 417	21	Helical; Potential
Amino acid modifications
	Modified residue	199	1	Phosphothreonine Ref.6
	Modified residue	228	1	Phosphoserine Ref.6
	Modified residue	231	1	Phosphoserine Ref.6
	Modified residue	233	1	Phosphoserine Ref.6
	Modified residue	234	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	425	1	N → K in AAT92609. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P38355 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: A2F947D0EA90E41C	FASTA	427	47,506
	10 20 30 40 50 60 MVETFSFAHL AYLVFESVLQ VVIIALAGFW SASSGLLPKQ SQKIISLLNV DLFTPCLIFS 70 80 90 100 110 120 KLAKSLSMAK IFEIAIIPIF FGLTTGISFI SGKIMSRILD LDKDETNFVV ANSVFGNSNS 130 140 150 160 170 180 LPVSLTLSLA YTLPNLTWDQ IPNDNRDNVA SRGILYLLIF QQIGQMLRWS WGYNKLMKWS 190 200 210 220 230 240 GENTQHMPPS QVQSLLERTP NIDNEELVNE EQEEQELLEE ENNRMNSSFL SSSSIGDKIW 250 260 270 280 290 300 QKSCTVFERI RANLNPPLYS MIFAVVVAAI GPLQRELFME DGFINNTFAE AVTQLGSVSI 310 320 330 340 350 360 PLILVVLGSN LYPSAEVFPK TVHHSKLLIG SIIGRMILPS CFLLPIIAIA VKYINVSILD 370 380 390 400 410 420 DPIFLVVGFL LTVSPPAIQL TQITQLNEFF EAEMADILFW GYAVLSLPVS IIVVSGAIYV LQWANPT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Holmstroem K., Brandt T., Kallesoe T. Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-199; SER-228; SER-231; SER-233 AND SER-234, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76053 Genomic DNA. Translation: CAA53650.1. Z36156 Genomic DNA. Translation: CAA85252.1. AY692590 Genomic DNA. Translation: AAT92609.1. BK006936 Genomic DNA. Translation: DAA07402.1.
PIR	S44549.
RefSeq	NP_009846.1. NM_001178635.1.
3D structure databases
ProteinModelPortal	P38355.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6660N.
IntAct	P38355. 5 interactions.
MINT	MINT-664487.
STRING	4932.YBR287W.
Proteomic databases
PaxDb	P38355.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR287W; YBR287W; YBR287W.
GeneID	852590.
KEGG	sce:YBR287W.
Organism-specific databases
CYGD	YBR287w.
SGD	S000000491. YBR287W.
Phylogenomic databases
eggNOG	COG0679.
HOGENOM	HOG000203886.
KO	K07088.
OMA	FDVGQEE.
OrthoDB	EOG4SN4XH.
Gene expression databases
Genevestigator	P38355.
GermOnline	YBR287W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004776. Auxin_eff. [Graphical view]
Pfam	PF03547. Mem_trans. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971751.

Entry information

Entry name

YB8B_YEAST

Accession

Primary (citable) accession number: P38355
Secondary accession number(s): D6VQT2, E9P8W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program