Sec sixty-one protein homolog - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P38353 (SSH1_YEAST)

Last modified February 9, 2010. Version 87. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Sec sixty-one protein homolog
Alternative name(s):
    Ssh1 complex subunit SSH1
    Ssh1 complex subunit alpha

Gene names

Name:	SSH1
Ordered Locus Names:	YBR283C
ORF Names:	YBR2020

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import. Ref.6
Subunit structure	Component of the heterotrimeric Ssh1 complex, which is composed of SSH1, SEB2 and SSS1.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Miscellaneous	Present with 704 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the secY/SEC61-alpha family.

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Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	SRP-dependent cotranslational protein targeting to membrane Ref.6 Inferred from genetic interaction. Source: SGD transmembrane transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Ssh1 translocon complex Ref.1 Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro signal sequence binding Inferred from direct assay. Source: SGD
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct
AGP1	P25376	1	EBI-18175,EBI-2357
CHO1	P08456	1	EBI-18175,EBI-14055
ERG11	P10614	1	EBI-18175,EBI-5127
ERG25	P53045	1	EBI-18175,EBI-6506
ERP1	Q05359	1	EBI-18175,EBI-6581
ERP2	P39704	1	EBI-18175,EBI-6587
ERP4	Q12450	1	EBI-18175,EBI-6598
ERV25	P54837	1	EBI-18175,EBI-6642
ERV29	P53337	1	EBI-18175,EBI-23662
GAP1	P19145	1	EBI-18175,EBI-7314
GOT1	Q03554	1	EBI-18175,EBI-2057821
ORM2	Q06144	1	EBI-18175,EBI-34916
OST1	P41543	1	EBI-18175,EBI-12651
PHO84	P25297	1	EBI-18175,EBI-13320
PHO88	P38264	1	EBI-18175,EBI-13350
PIS1	P06197	1	EBI-18175,EBI-13458
PMP2	P40975	1	EBI-18175,EBI-2043041
PMP3	P87284	1	EBI-18175,EBI-13555
RTN1	Q04947	1	EBI-18175,EBI-38020
SAC1	P32368	1	EBI-18175,EBI-16210
SCS7	Q03529	1	EBI-18175,EBI-16747
SPF1	P39986	1	EBI-18175,EBI-3128
SWP1	Q02795	1	EBI-18175,EBI-12666
VHS3	Q08438	1	EBI-18175,EBI-30482
YER053C-A	Q3E7B0	1	EBI-18175,EBI-2047907

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 490

490

Sec sixty-one protein homolog

PRO_0000131811

Regions

Topological domain

1 – 32

Cytoplasmic Potential

Transmembrane

33 – 53

Potential

Topological domain

54 – 121

Lumenal Potential

Transmembrane

122 – 142

Potential

Topological domain

143 – 146

Cytoplasmic Potential

Transmembrane

147 – 167

Potential

Topological domain

168 – 174

Lumenal Potential

Transmembrane

175 – 195

Potential

Topological domain

196 – 242

Cytoplasmic Potential

Transmembrane

243 – 263

Potential

Topological domain

264 – 293

Lumenal Potential

Transmembrane

294 – 314

Potential

Topological domain

315 – 339

Cytoplasmic Potential

Transmembrane

340 – 360

Potential

Topological domain

361

Lumenal Potential

Transmembrane

362 – 382

Potential

Topological domain

383 – 421

Cytoplasmic Potential

Transmembrane

422 – 442

Potential

Topological domain

443 – 449

Lumenal Potential

Transmembrane

450 – 470

Potential

Topological domain

471 – 490

Cytoplasmic Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

P38353-1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 48267EEA3E0191BD
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FASTA

490

53,312

        10         20         30         40         50         60 
MSGFRLIDIV KPILPILPEV ELPFEKLPFD DKIVYTIFAG LIYLFAQFPL VGLPKATTPN 

        70         80         90        100        110        120 
VNDPIYFLRG VFGCEPRTLL EFGLFPNISS GLILQLLAGL KVIKVNFKIQ SDRELFQSLT 

       130        140        150        160        170        180 
KVFAIVQYVI LTNIFIFAGY FGDDLSVVQI GLINFQLVGA GIFTTLLAEV IDKGFGFSSG 

       190        200        210        220        230        240 
AMIINTVVIA TNLVADTFGV SQIKVGEDDQ TEAQGALINL IQGLRSKHKT FIGGIISAFN 

       250        260        270        280        290        300 
RDYLPNLTTT IIVLAIAIIV CYLQSVRVEL PIRSTRARGT NNVYPIKLLY TGCLSVLFSY 

       310        320        330        340        350        360 
TILFYIHIFA FVLIQLVAKN EPTHIICKIM GHYENANNLL AVPTFPLSLL APPTSFFKGV 

       370        380        390        400        410        420 
TQQPLTFITY SAFILVTGIW FADKWQAISG SSARDVALEF KDQGITLMGR REQNVAKELN 

       430        440        450        460        470        480 
KVIPIAAVTG ASVLSLITVI GESLGLKGKA AGIVVGIAGG FSLLEVITIE YQQSGGQSAL 

       490 
NQVLGVPGAM

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae." Finke K., Plath K., Panzner S., Prehn S., Rapoport T.A., Hartmann E., Sommer T. EMBO J. 15:1482-1494(1996) [PubMed: 8612571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Holmstroem K., Brandt T., Kallesoe T. Yeast 10:S47-S62(1994) [PubMed: 8091861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast." Graack H.-R., Grohmann L., Kitakawa M. Biochimie 73:837-844(1991) [PubMed: 1764528] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-490.
[6]	"Ssh1p determines the translocation and dislocation capacities of the yeast endoplasmic reticulum." Wilkinson B.M., Tyson J.R., Stirling C.J. Dev. Cell 1:401-409(2001) [PubMed: 11702951] [Abstract] Cited for: FUNCTION.
[7]	"Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA." Prinz A., Behrens C., Rapoport T.A., Hartmann E., Kalies K.-U. EMBO J. 19:1900-1906(2000) [PubMed: 10775273] [Abstract] Cited for: ASSOCIATION OF THE SSH1 COMPLEX WITH RIBOSOMES.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U05336 Genomic DNA. Translation: AAB40986.1.
X76053 Genomic DNA. Translation: CAA53646.1.
Z36152 Genomic DNA. Translation: CAA85247.1.
AY692997 Genomic DNA. Translation: AAT93016.1.
S77888 Genomic DNA. Translation: AAB21097.2. Sequence problems.

PIR

S44545.

RefSeq

NP_009842.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WW9	electron microscopy	8.60	A	1-490	[»]
2WWA	electron microscopy	8.90	A	1-490	[»]

SMR

P38353. Positions 9-467.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4962N.

IntAct

P38353. 35 interactions.

STRING

P38353.

Proteomic databases

PeptideAtlas

P38353.

Genome annotation databases

Ensembl

YBR283C; YBR283C; YBR283C; Saccharomyces cerevisiae. [Genome view]

GeneID

852586.

KEGG

sce:YBR283C.

NMPDR

fig|4932.3.peg.558.

Organism-specific databases

CYGD

YBR283c.

SGD

S000000487. SSH1.

Phylogenomic databases

eggNOG

HOGENOM

OMA

OrthoDB

PhylomeDB

P38353.

Gene expression databases

ArrayExpress

P38353.

Genevestigator

P38353.

GermOnline

YBR283C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR002208. SecY.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]

PANTHER

PTHR10906. SecY. 1 hit.

Pfam

PF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]

PIRSF

PIRSF004557. SecY. 1 hit.

PROSITE

PS00755. SECY_1. 1 hit.
PS00756. SECY_2. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

971739.

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Entry information

Entry name

SSH1_YEAST

Accession

Primary (citable) accession number: P38353

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents