Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P38353 (SSH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sec sixty-one protein homolog
Alternative name(s):
Ssh1 complex subunit SSH1
Ssh1 complex subunit alpha
Gene names
Name:SSH1
Ordered Locus Names:YBR283C
ORF Names:YBR2020
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import. Ref.7

Subunit structure

Component of the heterotrimeric Ssh1 complex, which is composed of SSH1, SBH2 and SSS1.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Miscellaneous

Present with 704 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SecY/SEC61-alpha family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Sec sixty-one protein homolog
PRO_0000131811

Regions

Topological domain1 – 3232Cytoplasmic Potential
Transmembrane33 – 5321Helical; Potential
Topological domain54 – 12168Lumenal Potential
Transmembrane122 – 14221Helical; Potential
Topological domain143 – 1464Cytoplasmic Potential
Transmembrane147 – 16721Helical; Potential
Topological domain168 – 1747Lumenal Potential
Transmembrane175 – 19521Helical; Potential
Topological domain196 – 24247Cytoplasmic Potential
Transmembrane243 – 26321Helical; Potential
Topological domain264 – 29330Lumenal Potential
Transmembrane294 – 31421Helical; Potential
Topological domain315 – 33925Cytoplasmic Potential
Transmembrane340 – 36021Helical; Potential
Topological domain3611Lumenal Potential
Transmembrane362 – 38221Helical; Potential
Topological domain383 – 42139Cytoplasmic Potential
Transmembrane422 – 44221Helical; Potential
Topological domain443 – 4497Lumenal Potential
Transmembrane450 – 47021Helical; Potential
Topological domain471 – 49020Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P38353 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 48267EEA3E0191BD

FASTA49053,312
        10         20         30         40         50         60 
MSGFRLIDIV KPILPILPEV ELPFEKLPFD DKIVYTIFAG LIYLFAQFPL VGLPKATTPN 

        70         80         90        100        110        120 
VNDPIYFLRG VFGCEPRTLL EFGLFPNISS GLILQLLAGL KVIKVNFKIQ SDRELFQSLT 

       130        140        150        160        170        180 
KVFAIVQYVI LTNIFIFAGY FGDDLSVVQI GLINFQLVGA GIFTTLLAEV IDKGFGFSSG 

       190        200        210        220        230        240 
AMIINTVVIA TNLVADTFGV SQIKVGEDDQ TEAQGALINL IQGLRSKHKT FIGGIISAFN 

       250        260        270        280        290        300 
RDYLPNLTTT IIVLAIAIIV CYLQSVRVEL PIRSTRARGT NNVYPIKLLY TGCLSVLFSY 

       310        320        330        340        350        360 
TILFYIHIFA FVLIQLVAKN EPTHIICKIM GHYENANNLL AVPTFPLSLL APPTSFFKGV 

       370        380        390        400        410        420 
TQQPLTFITY SAFILVTGIW FADKWQAISG SSARDVALEF KDQGITLMGR REQNVAKELN 

       430        440        450        460        470        480 
KVIPIAAVTG ASVLSLITVI GESLGLKGKA AGIVVGIAGG FSLLEVITIE YQQSGGQSAL 

       490 
NQVLGVPGAM 

« Hide

References

« Hide 'large scale' references
[1]"A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae."
Finke K., Plath K., Panzner S., Prehn S., Rapoport T.A., Hartmann E., Sommer T.
EMBO J. 15:1482-1494(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
Holmstroem K., Brandt T., Kallesoe T.
Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast."
Graack H.-R., Grohmann L., Kitakawa M.
Biochimie 73:837-844(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-490.
[7]"Ssh1p determines the translocation and dislocation capacities of the yeast endoplasmic reticulum."
Wilkinson B.M., Tyson J.R., Stirling C.J.
Dev. Cell 1:401-409(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA."
Prinz A., Behrens C., Rapoport T.A., Hartmann E., Kalies K.-U.
EMBO J. 19:1900-1906(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION OF THE SSH1 COMPLEX WITH RIBOSOMES.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U05336 Genomic DNA. Translation: AAB40986.1.
X76053 Genomic DNA. Translation: CAA53646.1.
Z36152 Genomic DNA. Translation: CAA85247.1.
AY692997 Genomic DNA. Translation: AAT93016.1.
S77888 Genomic DNA. Translation: AAB21097.2. Sequence problems.
BK006936 Genomic DNA. Translation: DAA07398.1.
PIRS44545.
RefSeqNP_009842.1. NM_001178631.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WW9electron microscopy8.60A1-490[»]
2WWAelectron microscopy8.90A1-490[»]
ProteinModelPortalP38353.
SMRP38353. Positions 1-490.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32977. 143 interactions.
DIPDIP-4962N.
IntActP38353. 36 interactions.
MINTMINT-570624.
STRING4932.YBR283C.

Proteomic databases

MaxQBP38353.
PaxDbP38353.
PeptideAtlasP38353.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR283C; YBR283C; YBR283C.
GeneID852586.
KEGGsce:YBR283C.

Organism-specific databases

CYGDYBR283c.
SGDS000000487. SSH1.

Phylogenomic databases

eggNOGCOG0201.
HOGENOMHOG000231249.
KOK10956.
OMASTKVRGM.
OrthoDBEOG70CRGV.

Enzyme and pathway databases

BioCycYEAST:G3O-29203-MONOMER.

Gene expression databases

GenevestigatorP38353.

Family and domain databases

Gene3D1.10.3370.10. 1 hit.
InterProIPR002208. SecY/SEC61-alpha.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]
PANTHERPTHR10906. PTHR10906. 1 hit.
PfamPF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]
PIRSFPIRSF004557. SecY. 1 hit.
SUPFAMSSF103491. SSF103491. 2 hits.
PROSITEPS00755. SECY_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38353.
NextBio971739.

Entry information

Entry nameSSH1_YEAST
AccessionPrimary (citable) accession number: P38353
Secondary accession number(s): D6VQS8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references