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P38353

- SSH1_YEAST

UniProt

P38353 - SSH1_YEAST

Protein

Sec sixty-one protein homolog

Gene

SSH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein transmembrane transporter activity Source: SGD
    3. signal sequence binding Source: SGD

    GO - Biological processi

    1. protein transmembrane transport Source: GOC
    2. SRP-dependent cotranslational protein targeting to membrane Source: SGD

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29203-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sec sixty-one protein homolog
    Alternative name(s):
    Ssh1 complex subunit SSH1
    Ssh1 complex subunit alpha
    Gene namesi
    Name:SSH1
    Ordered Locus Names:YBR283C
    ORF Names:YBR2020
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR283c.
    SGDiS000000487. SSH1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. Ssh1 translocon complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Sec sixty-one protein homologPRO_0000131811Add
    BLAST

    Proteomic databases

    MaxQBiP38353.
    PaxDbiP38353.
    PeptideAtlasiP38353.

    Expressioni

    Gene expression databases

    GenevestigatoriP38353.

    Interactioni

    Subunit structurei

    Component of the heterotrimeric Ssh1 complex, which is composed of SSH1, SBH2 and SSS1.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ERV29P533373EBI-18175,EBI-23662
    PHO88P382643EBI-18175,EBI-13350
    SSS1P351798EBI-18175,EBI-16406

    Protein-protein interaction databases

    BioGridi32977. 143 interactions.
    DIPiDIP-4962N.
    IntActiP38353. 36 interactions.
    MINTiMINT-570624.
    STRINGi4932.YBR283C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2WW9electron microscopy8.60A1-490[»]
    2WWAelectron microscopy8.90A1-490[»]
    ProteinModelPortaliP38353.
    SMRiP38353. Positions 1-490.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38353.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3232CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini54 – 12168LumenalSequence AnalysisAdd
    BLAST
    Topological domaini143 – 1464CytoplasmicSequence Analysis
    Topological domaini168 – 1747LumenalSequence Analysis
    Topological domaini196 – 24247CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini264 – 29330LumenalSequence AnalysisAdd
    BLAST
    Topological domaini315 – 33925CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini361 – 3611LumenalSequence Analysis
    Topological domaini383 – 42139CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini443 – 4497LumenalSequence Analysis
    Topological domaini471 – 49020CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei33 – 5321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei122 – 14221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei147 – 16721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei294 – 31421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei340 – 36021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei362 – 38221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei422 – 44221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei450 – 47021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SecY/SEC61-alpha family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0201.
    HOGENOMiHOG000231249.
    KOiK10956.
    OMAiSTKVRGM.
    OrthoDBiEOG70CRGV.

    Family and domain databases

    Gene3Di1.10.3370.10. 1 hit.
    InterProiIPR002208. SecY/SEC61-alpha.
    IPR023201. SecY_su_dom.
    IPR019561. Translocon_Sec61/SecY_plug_dom.
    [Graphical view]
    PANTHERiPTHR10906. PTHR10906. 1 hit.
    PfamiPF10559. Plug_translocon. 1 hit.
    PF00344. SecY. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004557. SecY. 1 hit.
    SUPFAMiSSF103491. SSF103491. 2 hits.
    PROSITEiPS00755. SECY_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38353-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGFRLIDIV KPILPILPEV ELPFEKLPFD DKIVYTIFAG LIYLFAQFPL    50
    VGLPKATTPN VNDPIYFLRG VFGCEPRTLL EFGLFPNISS GLILQLLAGL 100
    KVIKVNFKIQ SDRELFQSLT KVFAIVQYVI LTNIFIFAGY FGDDLSVVQI 150
    GLINFQLVGA GIFTTLLAEV IDKGFGFSSG AMIINTVVIA TNLVADTFGV 200
    SQIKVGEDDQ TEAQGALINL IQGLRSKHKT FIGGIISAFN RDYLPNLTTT 250
    IIVLAIAIIV CYLQSVRVEL PIRSTRARGT NNVYPIKLLY TGCLSVLFSY 300
    TILFYIHIFA FVLIQLVAKN EPTHIICKIM GHYENANNLL AVPTFPLSLL 350
    APPTSFFKGV TQQPLTFITY SAFILVTGIW FADKWQAISG SSARDVALEF 400
    KDQGITLMGR REQNVAKELN KVIPIAAVTG ASVLSLITVI GESLGLKGKA 450
    AGIVVGIAGG FSLLEVITIE YQQSGGQSAL NQVLGVPGAM 490
    Length:490
    Mass (Da):53,312
    Last modified:October 1, 1994 - v1
    Checksum:i48267EEA3E0191BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05336 Genomic DNA. Translation: AAB40986.1.
    X76053 Genomic DNA. Translation: CAA53646.1.
    Z36152 Genomic DNA. Translation: CAA85247.1.
    AY692997 Genomic DNA. Translation: AAT93016.1.
    S77888 Genomic DNA. Translation: AAB21097.2. Sequence problems.
    BK006936 Genomic DNA. Translation: DAA07398.1.
    PIRiS44545.
    RefSeqiNP_009842.1. NM_001178631.1.

    Genome annotation databases

    EnsemblFungiiYBR283C; YBR283C; YBR283C.
    GeneIDi852586.
    KEGGisce:YBR283C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05336 Genomic DNA. Translation: AAB40986.1 .
    X76053 Genomic DNA. Translation: CAA53646.1 .
    Z36152 Genomic DNA. Translation: CAA85247.1 .
    AY692997 Genomic DNA. Translation: AAT93016.1 .
    S77888 Genomic DNA. Translation: AAB21097.2 . Sequence problems.
    BK006936 Genomic DNA. Translation: DAA07398.1 .
    PIRi S44545.
    RefSeqi NP_009842.1. NM_001178631.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2WW9 electron microscopy 8.60 A 1-490 [» ]
    2WWA electron microscopy 8.90 A 1-490 [» ]
    ProteinModelPortali P38353.
    SMRi P38353. Positions 1-490.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32977. 143 interactions.
    DIPi DIP-4962N.
    IntActi P38353. 36 interactions.
    MINTi MINT-570624.
    STRINGi 4932.YBR283C.

    Proteomic databases

    MaxQBi P38353.
    PaxDbi P38353.
    PeptideAtlasi P38353.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR283C ; YBR283C ; YBR283C .
    GeneIDi 852586.
    KEGGi sce:YBR283C.

    Organism-specific databases

    CYGDi YBR283c.
    SGDi S000000487. SSH1.

    Phylogenomic databases

    eggNOGi COG0201.
    HOGENOMi HOG000231249.
    KOi K10956.
    OMAi STKVRGM.
    OrthoDBi EOG70CRGV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29203-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38353.
    NextBioi 971739.

    Gene expression databases

    Genevestigatori P38353.

    Family and domain databases

    Gene3Di 1.10.3370.10. 1 hit.
    InterProi IPR002208. SecY/SEC61-alpha.
    IPR023201. SecY_su_dom.
    IPR019561. Translocon_Sec61/SecY_plug_dom.
    [Graphical view ]
    PANTHERi PTHR10906. PTHR10906. 1 hit.
    Pfami PF10559. Plug_translocon. 1 hit.
    PF00344. SecY. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004557. SecY. 1 hit.
    SUPFAMi SSF103491. SSF103491. 2 hits.
    PROSITEi PS00755. SECY_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae."
      Finke K., Plath K., Panzner S., Prehn S., Rapoport T.A., Hartmann E., Sommer T.
      EMBO J. 15:1482-1494(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
      Holmstroem K., Brandt T., Kallesoe T.
      Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast."
      Graack H.-R., Grohmann L., Kitakawa M.
      Biochimie 73:837-844(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-490.
    7. "Ssh1p determines the translocation and dislocation capacities of the yeast endoplasmic reticulum."
      Wilkinson B.M., Tyson J.R., Stirling C.J.
      Dev. Cell 1:401-409(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA."
      Prinz A., Behrens C., Rapoport T.A., Hartmann E., Kalies K.-U.
      EMBO J. 19:1900-1906(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION OF THE SSH1 COMPLEX WITH RIBOSOMES.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiSSH1_YEAST
    AccessioniPrimary (citable) accession number: P38353
    Secondary accession number(s): D6VQS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 704 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3