Sec sixty-one protein homolog - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38353 (SSH1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sec sixty-one protein homolog

Alternative name(s):
Ssh1 complex subunit SSH1
Ssh1 complex subunit alpha

Gene names

Name:	SSH1
Ordered Locus Names:	YBR283C
ORF Names:	YBR2020

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the Ssh1 complex, which probably is the major component of a channel-forming translocon complex that may function exclusively in the cotranslational pathway of protein endoplasmic reticulum (ER) import. Ref.7
Subunit structure	Component of the heterotrimeric Ssh1 complex, which is composed of SSH1, SBH2 and SSS1.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Miscellaneous	Present with 704 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SecY/SEC61-alpha family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	SRP-dependent cotranslational protein targeting to membrane Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	Ssh1 translocon complex Inferred from direct assay Ref.1. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	protein transmembrane transporter activity Inferred from sequence alignment Ref.1. Source: SGD signal sequence binding Inferred from direct assay PubMed 12134063. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSS1	P35179	8	EBI-18175,EBI-16406

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 490

490

Sec sixty-one protein homolog

PRO_0000131811

Regions

Topological domain

1 – 32

Cytoplasmic Potential

Transmembrane

33 – 53

Helical; Potential

Topological domain

54 – 121

Lumenal Potential

Transmembrane

122 – 142

Helical; Potential

Topological domain

143 – 146

Cytoplasmic Potential

Transmembrane

147 – 167

Helical; Potential

Topological domain

168 – 174

Lumenal Potential

Transmembrane

175 – 195

Helical; Potential

Topological domain

196 – 242

Cytoplasmic Potential

Transmembrane

243 – 263

Helical; Potential

Topological domain

264 – 293

Lumenal Potential

Transmembrane

294 – 314

Helical; Potential

Topological domain

315 – 339

Cytoplasmic Potential

Transmembrane

340 – 360

Helical; Potential

Topological domain

361

Lumenal Potential

Transmembrane

362 – 382

Helical; Potential

Topological domain

383 – 421

Cytoplasmic Potential

Transmembrane

422 – 442

Helical; Potential

Topological domain

443 – 449

Lumenal Potential

Transmembrane

450 – 470

Helical; Potential

Topological domain

471 – 490

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P38353 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 48267EEA3E0191BD
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FASTA

490

53,312

        10         20         30         40         50         60 
MSGFRLIDIV KPILPILPEV ELPFEKLPFD DKIVYTIFAG LIYLFAQFPL VGLPKATTPN 

        70         80         90        100        110        120 
VNDPIYFLRG VFGCEPRTLL EFGLFPNISS GLILQLLAGL KVIKVNFKIQ SDRELFQSLT 

       130        140        150        160        170        180 
KVFAIVQYVI LTNIFIFAGY FGDDLSVVQI GLINFQLVGA GIFTTLLAEV IDKGFGFSSG 

       190        200        210        220        230        240 
AMIINTVVIA TNLVADTFGV SQIKVGEDDQ TEAQGALINL IQGLRSKHKT FIGGIISAFN 

       250        260        270        280        290        300 
RDYLPNLTTT IIVLAIAIIV CYLQSVRVEL PIRSTRARGT NNVYPIKLLY TGCLSVLFSY 

       310        320        330        340        350        360 
TILFYIHIFA FVLIQLVAKN EPTHIICKIM GHYENANNLL AVPTFPLSLL APPTSFFKGV 

       370        380        390        400        410        420 
TQQPLTFITY SAFILVTGIW FADKWQAISG SSARDVALEF KDQGITLMGR REQNVAKELN 

       430        440        450        460        470        480 
KVIPIAAVTG ASVLSLITVI GESLGLKGKA AGIVVGIAGG FSLLEVITIE YQQSGGQSAL 

       490 
NQVLGVPGAM

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae." Finke K., Plath K., Panzner S., Prehn S., Rapoport T.A., Hartmann E., Sommer T. EMBO J. 15:1482-1494(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Holmstroem K., Brandt T., Kallesoe T. Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The nuclear coded mitoribosomal proteins YmL27 and YmL31 are both essential for mitochondrial function in yeast." Graack H.-R., Grohmann L., Kitakawa M. Biochimie 73:837-844(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-490.
[7]	"Ssh1p determines the translocation and dislocation capacities of the yeast endoplasmic reticulum." Wilkinson B.M., Tyson J.R., Stirling C.J. Dev. Cell 1:401-409(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA." Prinz A., Behrens C., Rapoport T.A., Hartmann E., Kalies K.-U. EMBO J. 19:1900-1906(2000) [PubMed] [Europe PMC] [Abstract] Cited for: ASSOCIATION OF THE SSH1 COMPLEX WITH RIBOSOMES.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U05336 Genomic DNA. Translation: AAB40986.1.
X76053 Genomic DNA. Translation: CAA53646.1.
Z36152 Genomic DNA. Translation: CAA85247.1.
AY692997 Genomic DNA. Translation: AAT93016.1.
S77888 Genomic DNA. Translation: AAB21097.2. Sequence problems.
BK006936 Genomic DNA. Translation: DAA07398.1.

PIR

S44545.

RefSeq

NP_009842.1. NM_001178631.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WW9	electron microscopy	8.60	A	1-490	[»]
2WWA	electron microscopy	8.90	A	1-490	[»]

ProteinModelPortal

P38353.

SMR

P38353. Positions 1-490.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-4962N.

IntAct

P38353. 18 interactions.

MINT

MINT-570624.

STRING

4932.YBR283C.

Proteomic databases

PaxDb

P38353.

PeptideAtlas

P38353.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR283C; YBR283C; YBR283C.

GeneID

852586.

KEGG

sce:YBR283C.

Organism-specific databases

CYGD

YBR283c.

SGD

S000000487. SSH1.

Phylogenomic databases

eggNOG

COG0201.

HOGENOM

HOG000231249.

K10956.

OMA

PIRLLYT.

OrthoDB

EOG4V46HD.

Gene expression databases

Genevestigator

P38353.

GermOnline

YBR283C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.10.3370.10. 1 hit.

InterPro

IPR002208. SecY/SEC61-alpha.
IPR023201. SecY_su_dom.
IPR019561. Translocon_Sec61/SecY_plug_dom.
[Graphical view]

PANTHER

PTHR10906. PTHR10906. 1 hit.

Pfam

PF10559. Plug_translocon. 1 hit.
PF00344. SecY. 1 hit.
[Graphical view]

PIRSF

PIRSF004557. SecY. 1 hit.

SUPFAM

SSF103491. SecY. 1 hit.

PROSITE

PS00755. SECY_1. 1 hit.
PS00756. SECY_2. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P38353.

NextBio

971739.

Entry information

Entry name

SSH1_YEAST

Accession

Primary (citable) accession number: P38353
Secondary accession number(s): D6VQS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	April 3, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents