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Reviewed, UniProtKB/Swiss-Prot P38352 (YB9W_YEAST)

Last modified June 16, 2009. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    F-box protein YBR280C
Gene names
Ordered Locus Names: YBR280C
ORF Names: YBR2017
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length637 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins By similarity.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with SKP1 and CDC53. Component of the probable SCF(YBR280C) complex containing CDC53, SKP1, RBX1 and YBR280C. Ref.3

Sequence similarities

Contains 1 F-box domain.

Contains 2 RCC1 repeats.

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Ontologies

Keywords
   Biological processUbl conjugation pathway
   DomainRepeat
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processSCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Inferred from mutant phenotype. Source: SGD

   Cellular componentSCF ubiquitin ligase complex

Inferred from mutant phenotype. Source: SGD

   Molecular functionprotein binding Ref.3

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

AAH1P539091EBI-21172,EBI-2197
CDC53Q120181EBI-21172,EBI-4321
SKP1P522861EBI-21172,EBI-4090

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 637637F-box protein YBR280C
PRO_0000206658

Regions

Domain14 – 6350F-box
Repeat109 – 20294RCC1 1
Repeat565 – 63571RCC1 2

Amino acid modifications

Modified residue161Phosphoserine Ref.5 Ref.6 Ref.7
Modified residue281Phosphoserine Ref.7
Modified residue291Phosphoserine Ref.6
Modified residue2661Phosphoserine Ref.7

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Sequences

Sequence LengthMass (Da)Tools
P38352-1 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 358D751C612744BE

FASTA63770,495
        10         20         30         40         50         60 
MSEVESREKE PDAGLSPDIV QATLPFLSSD DIKNLSQTNK YYNTLLDFDH SKILWHELFH 

        70         80         90        100        110        120 
KAFGTLKTND EPFQGRNSAE FKTCTETILR EAFPTLSWQE VYQLRAYDAK FYSWGYLKHG 

       130        140        150        160        170        180 
RLGYTASSNN ELVATSLNGP SPRFKYGVNT PTEVPWFNSR TTSRTSNFTP SEDPLSAIKK 

       190        200        210        220        230        240 
DGDEIIAQVS SGGFSFQILT ESGNLYSSGS TFSGGLKGPG PSGSQHDYNP FREMIHNMER 

       250        260        270        280        290        300 
SYPRITSRSN GSTVNTTGTF SGRRMSGSHP STAYEPGNAT TAQHITIDSG GAPAASPGGS 

       310        320        330        340        350        360 
HSGVPRTTMP SMGPHENIYS QIEMLERSAN KAVPGNNHIR RMFARNSFPL YSGRDENLGS 

       370        380        390        400        410        420 
FNDIQFVAVS SGRSHFLAMD TDNNIYSWDS TESDQGVKIE FANLPSRATN PILKIASGWN 

       430        440        450        460        470        480 
FNCCYIYKVG LVAWKERDAI QKGESFAFAK YEIVPNTNDV NGDSRIVDFA CLQDNCVFFI 

       490        500        510        520        530        540 
NNNGDKLWKY HNGLNQIVDL NIVGKLCKIN ACFASLVLFT DTHCYTLKVT NGDVDKDSLT 

       550        560        570        580        590        600 
ELDINENVIS VASGDYHTVA LTERGHLYSW GIESQDCGCL GLGPSEKIVN ELHIGNWEGQ 

       610        620        630 
RNIRVVKPTK IELPEDYICV SVTAGGWQTG ALIIKKH

« Hide

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References

« Hide 'large scale' references
[1]"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
Holmstroem K., Brandt T., Kallesoe T.
Yeast 10:S47-S62(1994) [PubMed: 8091861] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly."
Seol J.H., Shevchenko A., Shevchenko A., Deshaies R.J.
Nat. Cell Biol. 3:384-391(2001) [PubMed: 11283612] [Abstract]
Cited for: INTERACTION WITH CDC53 AND SKP1, IDENTIFICATION BY MASS SPECTROMETRY, DOMAIN F-BOX.
[4]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed: 12748633] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, MASS SPECTROMETRY.
[6]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-29, MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16; SER-28 AND SER-266, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X76053 Genomic DNA. Translation: CAA53643.1. Different initiation.
Z36149 Genomic DNA. Translation: CAA85244.1. Different initiation.
PIRS44542.
RefSeqNP_009839.2.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6307N.
IntActP38352. 5 interactions.

Proteomic databases

PRIDEP38352.

Genome annotation databases

EnsemblYBR280C. Saccharomyces cerevisiae. [Contig view]
GeneID852583.
GenomeReviewsGene locus YBR280C in contig Y13134_GR.
KEGGsce:YBR280C.
NMPDRfig|4932.3.peg.555.

Organism-specific databases

CYGDYBR280c.
SGDS000000484. SAF1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP38352.
OMAP38352. GFSFQIL.

Gene expression databases

GermOnlineYBR280C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000408. Reg_chr_condens.
IPR009091. Reg_csome_cond/b-lactamase_inh.
[Graphical view]
Gene3DG3DSA:2.130.10.30. Reg_csome_cond/b-lactamase_inh. 1 hit.
PROSITEPS50181. FBOX. False negative.
PS00625. RCC1_1. False negative.
PS00626. RCC1_2. 1 hit.
PS50012. RCC1_3. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio971730.

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Entry information

Entry nameYB9W_YEAST
AccessionPrimary (citable) accession number: P38352
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 16, 2006
Last modified: June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents